Eng
Material Safety Data Sheet
Conforms to 93/112/EC and ISO 11014-1
Responsible Name Company2
Section 1. Chemical Product and Company Identification
Product name
Phenol : Chloroform Isoamyl Alcohol Kit
Product No. 0017908 0017909
Supplier In USA: In Europe: Manufacturer Pierce Biotechnology
Pierce Perbio Science P.O. Box 117
P.O. Box 117 Industriezone III Rockford, IL 61105
Rockford, IL 61105 Industrielaan 27 USA
USA 9320 Erembodegem-Aalst 815.968.0747 or
815.968.0747 or Belgium 1.800.874.3723
1.800.874.3723 Tel:+32 53 83 44 04
Fax:+32 53 83 76 38
In Case of Emergency CHEMTREC: Print Date 6/1/2005
800.424.9300 Validation Date 6/1/2005
OUTSIDE US:
MSDS# 1355
202.483.7616
Intended Use Refer to the instruction booklet for proper and intended use. Otherwise, contact supplier for specific applications.
Section 2. Composition, Information on Ingredients
Substance/Preparation Preparation
:
Ingredient name CAS number % EC number Classification
Phenol 108-95-2 45 - 65 203-632-7 T; R24/25
C; R34
Chloroform 67-66-3 45 - 65 200-663-8 Carc. Cat. 3; R40
Xn; R22, 48/20/22
Xi; R38
Isoamyl Alcohol 123-51-3 1-3 204-633-5 R10
Xn; R22
Xi; R36/37/38
Section 3. Hazards Identification
United States Review the most current and approved institutional guideline, protocol, standard operating procedure(s) and MSDS(s)
for the proper handling of institutional materials/equipment associated with the use of this product.
Emergency Overview Danger!
MAY BE FATAL IF INHALED, ABSORBED THROUGH SKIN OR SWALLOWED.
CAUSES RESPIRATORY TRACT, EYE AND SKIN BURNS.
CONTAINS MATERIAL WHICH CAUSES DAMAGE TO THE FOLLOWING ORGANS:
KIDNEYS, LUNGS, NERVOUS SYSTEM, LIVER, HEART, SPLEEN, RESPIRATORY
TRACT, SKIN, CENTRAL NERVOUS SYSTEM, EYE, LENS OR CORNEA,
PANCREAS.
SUSPECT CANCER HAZARD
CONTAINS MATERIAL WHICH MAY CAUSE CANCER
Do not ingest. Do not get in eyes, on skin or clothing. Do not breathe vapor or mist. Keep container closed. Use only
with adequate ventilation. Wash thoroughly after handling. Risk of cancer depends on duration and level of exposure.
Target organs Contains material which causes damage to the following organs: kidneys, lungs, the nervous
system, liver, heart, spleen, upper respiratory tract, skin, central nervous system (CNS), eye, lens
or cornea, pancreas.
Routes of Entry Dermal contact. Eye contact. Inhalation. Ingestion.
Potential acute health effects
Eyes Corrosive to eyes.
Skin Very toxic in contact with skin. Corrosive to the skin.
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Phenol : Chloroform Isoamyl Alcohol
Kit
Inhalation Very toxic by inhalation. Corrosive to the respiratory system.
Ingestion Very toxic if swallowed. May cause burns to mouth, throat and stomach.
Potential chronic health effects
Carcinogenic Effects Data CARCINOGENIC EFFECTS: Classified A4 (Not classifiable for human or animal.) by ACGIH, 3 (Not classifiable for
human.) by IARC, D (Not classifiable for human or animal.) by EPA [Phenol]. Classified + (Proven.) by NIOSH
[Chloroform]. Classified A3 (Proven for animal.) by ACGIH, 2B (Possible for human.) by IARC [Chloroform]. Classified
None. by NIOSH [Isoamyl Alcohol].
MUTAGENIC EFFECTS: Not available.
TERATOGENIC EFFECTS: Not available.
Medical Conditions Aggravated Repeated or prolonged contact with spray mist may produce chronic eye irritation and severe skin irritation. Repeated or
by Overexposure: prolonged exposure to spray mist may produce respiratory tract irritation leading to frequent attacks of bronchial infection.
Overexposure /Signs/Symptoms Repeated exposure to a highly toxic material may produce general deterioration of health by an accumulation in one or
many human organs.
Not available.
Europe
Classification Carc. Cat. 3; R40
T; R24/25
Xn; R48/20/22
C; R34
Physical/chemical hazards Not applicable.
Human health hazards Toxic in contact with skin and if swallowed.
Causes burns.
Possible risk of irreversible effects.
Harmful: danger of serious damage to health by prolonged exposure through inhalation and if swallowed.
Environmental hazards Not applicable.
See Toxicological Information (section 11)
Section 4. First Aid Measures
Notice to Reader Get immediate medical attention.
Effects and symptoms
Inhalation Hazardous in case of inhalation (lung corrosive). Inhalation of the spray mist may produce severe irritation of respiratory
tract, characterized by coughing, choking, or shortness of breath. Over-exposure by inhalation may cause respiratory
irritation.
Ingestion May be fatal if swallowed. May cause burns to mouth, throat and stomach.
Skin Contact Sensitization of the product: Not available.
Very hazardous in case of skin contact (irritant, permeator). Hazardous in case of skin contact (corrosive). Skin contact
may produce burns. Severe over-exposure can result in death. Skin inflammation is characterized by itching, scaling,
reddening, or, occasionally, blistering.
Eye Contact Very hazardous in case of eye contact (irritant). Hazardous in case of eye contact (corrosive). Inflammation of the eye is
characterized by redness, watering, and itching.
Aggravating conditions Repeated or prolonged contact with spray mist may produce chronic eye irritation and severe skin irritation. Repeated or
prolonged exposure to spray mist may produce respiratory tract irritation leading to frequent attacks of bronchial infection.
Repeated exposure to a highly toxic material may produce general deterioration of health by an accumulation in one or many
human organs.
First-Aid Measures
Inhalation In case of contact, immediately flush eyes with plenty of water for at least 15 minutes. Get medical attention immediately.
Ingestion Do NOT induce vomiting unless directed to do so by medical personnel. Never give anything by mouth to an unconscious
person. Get medical attention immediately.
Skin Contact In case of contact, immediately flush skin with plenty of water for at least 15 minutes while removing contaminated clothing
and shoes. Wash clothing before reuse. Thoroughly clean shoes before reuse. Get medical attention immediately.
Eye Contact In case of contact, immediately flush eyes with plenty of water for at least 15 minutes. Get medical attention immediately.
Notes to Physician Not available.
Protection of first-aiders Not available.
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Phenol : Chloroform Isoamyl Alcohol
Kit
Section 5. Fire Fighting Measures
Flammability of the Product Flammable.
Flash Points The lowest known value is Closed cup: 41.9癈 (107.4癋). (Isoamyl Alcohol)
Fire Hazards in Presence of Various Not available.
Substances
Fire Fighting Media Use an extinguishing agent suitable for surrounding fires.
and Instructions
Protective Clothing (Fire) Be sure to use an approved/certified respirator or equivalent.
Special Remarks on Fire Hazards Combustible material: may burn but does not ignite readily.
When heated, vapors may form explosive mixtures with air: indoors, outdoors, and sewers explosion hazards.
Some may polymerize (P) explosively when heated or involved in a fire.
Contact with metals may evolve flammable hydrogen gas.
Containers may explode when heated.
Runoff may pollute waterways.
Substance may be transported in a molten form. (Phenol)
Hazardous thermal (de)composition These products are carbon oxides (CO, CO2), halogenated compounds, hydrogen chloride.
products
Section 6. Accidental Release Measures
Personal precautions Face shield. Full suit. Vapor respirator. Be sure to use an approved/certified respirator or equivalent. Gloves. Boots.
Environmental Precautions and Keep away from heat. Keep away from sources of ignition. Stop leak if without risk. Absorb with DRY earth, sand or
Clean-up Methods other non-combustible material. Do not get water inside container. Do not touch spilled material. Use water spray
curtain to divert vapor drift. Use water spray to reduce vapors. Prevent entry into sewers, basements or confined
areas; dike if needed. Call for assistance on disposal. Neutralize the residue with a dilute solution of sodium
carbonate.
Small Spill and Leak Dilute with water and mop up, or absorb with an inert dry material and place in an appropriate waste disposal container.
If necessary: Neutralize the residue with a dilute solution of sodium carbonate.
Section 7. Handling and Storage
Handling Do not ingest. Do not get in eyes, on skin or on clothing. Keep container closed. Use only with adequate ventilation.
Do not breathe vapor or mist. Wash thoroughly after handling.
Storage Keep container tightly closed. Keep container in a cool, well-ventilated area.
Intended Use Refer to the instruction booklet for proper and intended use. Otherwise, contact supplier for specific applications.
Packaging materials
Suitable / Not suitable Use original container.
Czech Republic - Storage II
code
Section 8. Exposure Controls/Personal Protection
Engineering Controls Provide exhaust ventilation or other engineering controls to keep the airborne concentrations of vapors below their
respective occupational exposure limits. Ensure that eyewash stations and safety showers are proximal to the work-
station location.
Exposure Limit Values
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Phenol : Chloroform Isoamyl Alcohol
Kit
Ingredient Name Occupational Exposure Limits
United States
Phenol ACGIH (United States, 0/1996). Skin
TWA: 19 mg/m3
OSHA (United States, 0/1996). Skin
TWA: 5 ppm
NIOSH (United States, 0/1994). Skin
TWA: 5 ppm
CEIL: 15.6 ppm
TWA: 19 mg/m3
CEIL: 60 mg/m3
OSHA (United States, 0/1989). Skin
TWA: 5 ppm
TWA: 19 mg/m3
ACGIH (United States, 0/1994).
Isoamyl Alcohol
TWA: 361 mg/m3
STEL: 452 mg/m3
OSHA (United States, 0/1989).
TWA: 361 mg/m3
STEL: 452 mg/m3
NIOSH REL (United States, 0/1994).
TWA: 100 ppm 8 hour(s).
Sweden
Chloroform AFS (Sweden, 0/1996).
NGV: 2 ppm
KTV: 25 ppm
TGV: 5 ppm
Denmark
Phenol DK-Arbejdstilsynet (Denmark, 0/1999). Skin
GV: 4 mg/m3
Chloroform DK-Arbejdstilsynet (Denmark, 0/1996).
GV: 2 ppm
Isoamyl Alcohol DK-Arbejdstilsynet (Denmark, 0/1996).
GV: 360 mg/m3
Norway
N-Arbeidstilsynet (Norway, 0/1996).
Chloroform
AN: 2 ppm
Isoamyl Alcohol N-Arbeidstilsynet (Norway, 0/1996).
AN: 180 mg/m3
Korttidsnormen: 450 mg/m3
France
Chloroform INRS (France, 0/1999).
VME: 5 ppm
VLE: 50 ppm
Isoamyl Alcohol INRS (France, 0/1996).
VME: 360 mg/m3
Netherlands
Phenol Arbeidsinspectie (Netherlands, 0/1999). Skin
TGG: 5 ppm
Chloroform Arbeidsinspectie (Netherlands, 0/2000).
TGG: 1 ppm
TGG 15 min: 5 ppm
Isoamyl Alcohol Arbeidsinspectie (Netherlands, 0/1999).
TGG: 360 mg/m3
Germany
BAUA (Germany, 0/1999).
Chloroform
TWA: 10 mg/m3
Spitzenbegrenzung: 40 mg/m3
Isoamyl Alcohol BAUA (Germany, 0/1997).
TWA: 360 mg/m3
STEL: 125 mg/m3
Spitzenbegrenzung: 1440 mg/m3
Finland
Phenol Tyoterveyslaitos (Finland, 0/1999). Skin
TWA: 5 ppm
Chloroform Tyoterveyslaitos (Finland, 0/1998).
TWA: 10 ppm
STEL: 20 ppm
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Phenol : Chloroform Isoamyl Alcohol
Kit
United Kingdom (UK)
EH40 (United Kingdom (UK), 0/1999). Skin
Phenol
TWA: 5 ppm
Austria
Phenol AUVA (Austria, 0/1999). Skin
TWA: 5 ppm
Chloroform AUVA (Austria, 0/1995).
TWA: 10 mg/m3
Spitzenbegrenzung: 100 mg/m3
Isoamyl Alcohol AUVA (Austria, 0/1995).
TWA: 360 mg/m3
Spitzenbegrenzung: 720 mg/m3
Switzerland
Chloroform SUVA (Switzerland, 0/1997).
MAK: 10 ppm 8 hour(s).
Kurzzeitsgrenzwerte: 20 ppm 15 minute(s).
Belgium
TWA: 19 mg/m3
Phenol
Chloroform TWA: 10 ppm
TWA: 366 mg/m3
Isoamyl Alcohol
STEL: 459 mg/m3
Turkey
Phenol NIOSH (United States, 0/1994). Skin
TWA: 5 ppm
CEIL: 15.6 ppm
TWA: 19 mg/m3
CEIL: 60 mg/m3
Isoamyl Alcohol NIOSH REL (United States, 0/1994).
TWA: 100 ppm 8 hour(s).
Personal Protection
Splash goggles.
Eyes
Lab coat.
Body
Gloves.
Hands
Respirator is not needed under normal and intended conditions of use, if exposures are kept below established limits.
Respiratory
Boots.
Feet
Protective Clothing
(Pictograms)
Section 9. Physical and Chemical Properties
Physical State and Liquid. Color Clear Yellow. Odor Not available.
Appearance
Taste Not available.
Molecular Weight Not applicable.
pH (1% Soln/Water) Acidic.
Boiling/Condensation Point The lowest known value is 60.55癈 (141癋) (Chloroform). Weighted average: 122.16癈 (251.9癋)
Melting/Freezing Point May start to solidify at 40.9癈 (105.6癋) based on data for: Phenol. Weighted average: -13.17癈 (8.3癋)
Critical Temperature The lowest known value is 263.3癈 (505.9癋) (Chloroform).
Specific Gravity Weighted average: 1.23 (Water = 1)
Vapor Pressure The highest known value is 21.3 kPa (160 mm Hg) (at 20癈) (Chloroform). Weighted average: 10.39 kPa (77.93 mm Hg)
(at 20癈)
Vapor Density The highest known value is 4.1 (Air = 1) (Chloroform). Weighted average: 3.65 (Air = 1)
Evaporation Rate 10.2 (Chloroform) compared to (n-BUTYL ACETATE=1)
Dispersion Properties See solubility in water.
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Phenol : Chloroform Isoamyl Alcohol
Kit
Solubility Partially soluble in cold water, hot water.
Flash point The lowest known value is Closed cup: 41.9癈 (107.4癋). (Isoamyl Alcohol)
Explosion limits The greatest known range is Lower: 1.2% Upper: 9% (Isoamyl Alcohol)
Section 10. Stability and Reactivity
Stability and Reactivity The product is stable.
Conditions to avoid Not available.
Materials to avoid Reactive with oxidizing agents, metals, acids, alkalis.
Hazardous Polymerization Will not occur.
Hazardous Decomposition These products are halogenated compounds, hydrogen chloride.
Products
Section 11. Toxicological Information
Toxicity to Animals Phenol:
ORAL (LD50): Acute: 300 mg/kg [Rat]. 2300 mg/kg [Mouse]. 500 mg/kg
[mammal (species unspecified)].
DERMAL (LD50): Acute: 669 mg/kg [rat]. 630 mg/kg [rabbit].
Chloroform:
ORAL (LD50): Acute: 695 mg/kg [Rat]. 36 mg/kg [Mouse]. 820 mg/kg
[Guinea pig].
Isoamyl Alcohol:
ORAL (LD50): Acute: 1300 mg/kg [Rat]. 3438 mg/kg [Rabbit].
Chronic Effects on Humans CARCINOGENIC EFFECTS: Classified A4 (Not classifiable for human or animal.) by ACGIH, 3 (Not classifiable for
human.) by IARC, D (Not classifiable for human or animal.) by EPA [Phenol]. Classified + (Proven.) by NIOSH
[Chloroform]. Classified A3 (Proven for animal.) by ACGIH, 2B (Possible for human.) by IARC [Chloroform]. Classified
None. by NIOSH [Isoamyl Alcohol].
Contains material which causes damage to the following organs: kidneys, lungs, the nervous system, liver, heart, spleen,
upper respiratory tract, skin, central nervous system (CNS), eye, lens or cornea, pancreas.
Other Toxic Effects on Humans Hazardous in case of eye contact (corrosive), of inhalation (lung corrosive).
Slightly hazardous in case of ingestion, of inhalation (lung irritant).
Special Remarks on Toxicity to Not available.
Animals
Special Remarks on Chronic Not available.
Effects on Humans
Special Remarks on Other Toxic Not available.
Effects on Humans
Carcinogenicity Contains material which may cause cancer based on animal data. Risk of cancer depends on
duration and level of exposure.
Mutagenicity No known significant effects or critical hazards.
Reproductive toxicity No known significant effects or critical hazards.
Over-exposure
signs/symptoms
Inhalation Harmful: danger of serious damage to health by prolonged exposure through inhalation.
Ingestion No known significant effects or critical hazards.
Skin No known significant effects or critical hazards.
Target organs Contains material which causes damage to the following organs: kidneys, lungs, the nervous
system, liver, heart, spleen, upper respiratory tract, skin, central nervous system (CNS), eye, lens
or cornea, pancreas.
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Phenol : Chloroform Isoamyl Alcohol
Kit
Section 12. Ecological Information
Mobility Not available.
Persistence/degradability Not available.
Bioaccumulative potential Not available.
Ecotoxicity Not available.
Germany water class VCI WGK: No products were found.
Ecotoxicity data
Ingredient name Species Period
Other ecological information
Persistence/degradability
Ingredient name BOD5 COD
Ingredient name Aquatic half-life Photolysis
Bioaccumulative potential
Ingredient name LogPow BCF
Mobility : Not available.
Other adverse effects : No known significant effects or critical hazards.
Section 13. Disposal Considerations
Waste Stream Not available.
Methods of disposal The generation of waste should be avoided or minimized wherever possible. Avoid dispersal of
spilled material and runoff and contact with soil, waterways, drains and sewers. Disposal of this
product, solutions and any by-products should at all times comply with the requirements of
environmental protection and waste disposal legislation and any regional local authority
requirements.
European waste catalogue Not available.
(EWC)
Hazardous waste The classification of the product may meet the criteria for a hazardous waste
Denmark ?Carcinogenic waste Not available.
Denmark - Waste card number Not available.
Denmark - Waste group Not available.
Sweden - thermoset plastic Not available.
waste
Sweden - Waste group Not available.
Austria - Waste catalogue Not available.
Norway - Waste number Not available.
Norway - Hazardous waste The classification of the product may meet the criteria for a hazardous waste
Switzerland - Waste code Not available.
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Phenol : Chloroform Isoamyl Alcohol
Kit
Section 14. Transport Information
Contact the supplier for all information regarding the proper transportation method for this material.
1
Section 15. Regulatory Information
Label Requirements
R40- Limited evidence of a carcinogenic effect. R24/25- Toxic in contact with skin and if swallowed.
(Europe)
R48/20/22- Harmful: danger of serious damage to health by prolonged exposure through inhalation and if
swallowed. R34- Causes burns.
S1/2- Keep locked up and out of the reach of children. S26- In case of contact with eyes, rinse
immediately with plenty of water and seek medical advice. S36/37/39- Wear suitable protective clothing,
gloves and eye/face protection. S45- In case of accident or if you feel unwell, seek medical advice
immediately (show the label where possible).
Toxic
HCS Classification Highly toxic material
Corrosive material
Carcinogen
Target organ effects
U.S. Federal Regulations TSCA 8(b) inventory: Phenol; Chloroform; Isoamyl Alcohol
TSCA 8(d) H and S data reporting: Phenol: 1987
SARA 302/304/311/312 extremely hazardous substances: Phenol; Chloroform
SARA 302/304 emergency planning and notification: Phenol; Chloroform
SARA 302/304/311/312 hazardous chemicals: Phenol; Chloroform; Isoamyl Alcohol
SARA 311/312 MSDS distribution - chemical inventory - hazard identification: Phenol: Fire hazard, Immediate (Acute) Health Hazard, Delayed
(Chronic) Health Hazard; Chloroform: Immediate (Acute) Health Hazard, Delayed (Chronic) Health Hazard; Isoamyl Alcohol: Fire hazard, Immediate
(Acute) Health Hazard
Clean Water Act (CWA) 307: Phenol; Chloroform
Clean Water Act (CWA) 311: Phenol; Chloroform
Clean air act (CAA) 112 accidental release prevention: Chloroform
Clean air act (CAA) 112 regulated flammable substances: No products were found.
Clean air act (CAA) 112 regulated toxic substances: Chloroform
State regulations Pennsylvania RTK: Phenol: (environmental hazard); Chloroform: (special hazard, environmental hazard,
generic environmental hazard); Isoamyl Alcohol: (generic environmental hazard)
Massachusetts RTK: Phenol; Chloroform; Isoamyl Alcohol
Massachusetts spill list: Chloroform
New Jersey: Phenol; Chloroform; Isoamyl Alcohol
WARNING: This product contains a chemical known to the State of California to cause cancer.
Ingredient name Cancer Reproductive No significant risk level Maximum acceptable
dosage level
Chloroform Yes. No. 20 礸/day (ingestion) No.
40 礸/day (inhalation)
WHMIS (Canada) Class D-1A: Material causing immediate and serious toxic effects (VERY TOXIC).
Class D-2B: Material causing other toxic effects (TOXIC).
Class E: Corrosive liquid.
CEPA DSL: Phenol; Chloroform; Isoamyl Alcohol
International Regulations
EINECS Not available.
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Phenol : Chloroform Isoamyl Alcohol
Kit
DSCL (EEC) R40- Limited evidence of a carcinogenic effect.
R24/25- Toxic in contact with skin and if swallowed.
R48/20/22- Harmful: danger of serious damage to health by prolonged exposure through inhalation and if swallowed.
R34- Causes burns.
International Lists Australia (NICNAS): Phenol; Chloroform; Isoamyl Alcohol
Germany water class: Chloroform
Japan (METI): Phenol; Chloroform; Isoamyl Alcohol
Korea (TCCL): Phenol; Chloroform; Isoamyl Alcohol
Philippines (RA6969): Phenol; Chloroform; Isoamyl Alcohol
State Regulations Pennsylvania RTK: Phenol: (environmental hazard); Chloroform: (special hazard, environmental hazard, generic environmental hazard); Isoamyl
Alcohol: (generic environmental hazard)
Massachusetts RTK: Phenol; Chloroform; Isoamyl Alcohol
Massachusetts spill list: Chloroform
New Jersey: Phenol; Chloroform; Isoamyl Alcohol
Section 16. Other Information
National Fire Protection
Hazardous Material * 3
Health Flammability
1
Association (U.S.A.)
Information System
1
(U.S.A.) Fire hazard 3 1
Health Instability
1
Reactivity
Special
C
Personal protection
References Not available.
History of Document Changes Any information changes since last document version are marked with a triangle symbol.
Full text of R phrases referred R10- Flammable.
to in sections 2 and 3 - R40- Limited evidence of a carcinogenic effect.
Europe R24/25- Toxic in contact with skin and if swallowed.
R22- Harmful if swallowed.
R48/20/22- Harmful: danger of serious damage to health by prolonged exposure through inhalation
and if swallowed.
R34- Causes burns.
R36/37/38- Irritating to eyes, respiratory system and skin.
R38- Irritating to skin.
Full text of classifications Carc. Cat.3 - Carcinogen Category 3
referred to in sections 2 and 3 T - Toxic
- Europe C - Corrosive
Xn - Harmful
Xi - Irritant
Intended Use Refer to the instruction booklet for proper and intended use. Otherwise, contact supplier for specific applications.
Date of Previous Issue No Previous
Validated by Company2 on 6/1/2005. Verified by Company2.
Validation
Printed 6/1/2005. Version 0.02
Notice to Reader
To the best of our knowledge, the information contained herein is accurate. However, neither the above named supplier nor any of its
subsidiaries assumes any liability whatsoever for the accuracy or completeness of the information contained herein.
Final determination of suitability of any material is the sole responsibility of the user. All materials may present unknown hazards and
should be used with caution. Although certain hazards are described herein, we cannot guarantee that these are the only hazards that
exist.
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