SIGMA-ALDRICH
MATERIAL SAFETY DATA SHEET
Date Printed: 03/04/2008
Date Updated: 01/27/2006
Version 1.5
Section 1 - Product and Company Information
Product Name PHENOL: CHLOROFORM 5:1 MOLECULAR
BIOLOGY REAGENT
Product Number P1944
Brand SIGMA
Company Sigma-Aldrich
Address 3050 Spruce Street
SAINT LOUIS MO 63103 US
Technical Phone: 800-325-5832
Fax: 800-325-5052
Emergency Phone: 314-776-6555
Section 2 - Composition/Information on Ingredient
Substance Name CAS # SARA 313
PHENOL: CHLOROFORM 5:1 MOLECULAR None No
BIOLOGY REAGENT
Ingredient Name CAS # Percent SARA 313
PHENOL 108-95-2 83 Yes
CHLOROFORM 67-66-3 16 Yes
3-METHYL-1-BUTANOL 123-51-3 0.7 No
Section 3 - Hazards Identification
EMERGENCY OVERVIEW
Toxic.
Toxic by inhalation, in contact with skin and if swallowed. Causes
burns. Limited evidence of a carcinogenic effect. Harmful: danger
of serious damage to health by prolonged exposure through
inhalation in contact with skin, and if swallowed. Possible risk
of irreversible effects.
Readily absorbed through skin. Vesicant. Combustible. Target
organ(s): Central nervous system. Liver. Calif. Prop. 65
carcinogen.
HMIS RATING
HEALTH: 3*
FLAMMABILITY: 2
REACTIVITY: 0
NFPA RATING
HEALTH: 3
FLAMMABILITY: 2
REACTIVITY: 0
*additional chronic hazards present.
For additional information on toxicity, please refer to Section 11.
Section 4 - First Aid Measures
ORAL EXPOSURE
If swallowed, wash out mouth with water provided person is
conscious. Call a physician. Do not induce vomiting.
INHALATION EXPOSURE
If inhaled, remove to fresh air. If not breathing give
artificial respiration. If breathing is difficult, give oxygen.
DERMAL EXPOSURE
In case of skin contact, flush with copious amounts of water for
at least 15 minutes. Remove contaminated clothing and shoes.
Call a physician.
EYE EXPOSURE
In case of contact with eyes, flush with copious amounts of
water for at least 15 minutes. Assure adequate flushing by
separating the eyelids with fingers. Call a physician.
Section 5 - Fire Fighting Measures
FLASH POINT
175 癋 79.44 癈 Method: closed cup
AUTOIGNITION TEMP
N/A
FLAMMABILITY
N/A
EXTINGUISHING MEDIA
Suitable: Carbon dioxide, dry chemical powder, or appropriate
foam.
FIREFIGHTING
Protective Equipment: Wear self-contained breathing apparatus
and protective clothing to prevent contact with skin and eyes.
Specific Hazard(s): Emits toxic fumes under fire conditions.
Combustible liquid.
Section 6 - Accidental Release Measures
PROCEDURE TO BE FOLLOWED IN CASE OF LEAK OR SPILL
Evacuate area.
PROCEDURE(S) OF PERSONAL PRECAUTION(S)
Wear self-contained breathing apparatus, rubber boots, and heavy
rubber gloves.
METHODS FOR CLEANING UP
Cover with dry lime or soda ash, pick up, keep in a closed
container, and hold for waste disposal. Ventilate area and wash
spill site after material pickup is complete.
Section 7 - Handling and Storage
HANDLING
User Exposure: Do not breathe vapor. Do not get in eyes, on
skin, on clothing. Avoid prolonged or repeated exposure.
STORAGE
Suitable: Keep tightly closed. Keep away from heat and open
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flame. Store in a cool dry place.
Store at 2-8癈
Section 8 - Exposure Controls / PPE
ENGINEERING CONTROLS
Safety shower and eye bath. Use only in a chemical fume hood.
PERSONAL PROTECTIVE EQUIPMENT
Respiratory: Use respirators and components tested and approved
under appropriate government standards such as NIOSH (US) or CEN
(EU). Where risk assessment shows air-purifying respirators are
appropriate use a full-face respirator with multi-purpose
combination (US) or type ABEK (EN 14387) respirator cartridges as
a backup to engineering controls. If the respirator is the sole
means of protection, use a full-face supplied air respirator.
Hand: Compatible chemical-resistant gloves.
Eye: Chemical safety goggles.
Other: Faceshield (8-inch minimum).
GENERAL HYGIENE MEASURES
Wash contaminated clothing before reuse. Discard contaminated
shoes. Wash thoroughly after handling.
Section 9 - Physical/Chemical Properties
Appearance Physical State: Liquid
Property Value At Temperature or Pressure
Molecular Weight N/A
pH N/A
BP/BP Range N/A
MP/MP Range N/A
Freezing Point N/A
Vapor Pressure N/A
Vapor Density N/A
Saturated Vapor Conc. N/A
SG/Density N/A
Bulk Density N/A
Odor Threshold N/A
Volatile% N/A
VOC Content N/A
Water Content N/A
Solvent Content N/A
Evaporation Rate N/A
Viscosity N/A
Surface Tension N/A
Partition Coefficient N/A
Decomposition Temp. N/A
Flash Point 175 癋 79.44 癈 Method: closed cup
Explosion Limits N/A
Flammability N/A
Autoignition Temp N/A
Refractive Index N/A
Optical Rotation N/A
Miscellaneous Data N/A
Solubility N/A
N/A = not available
Section 10 - Stability and Reactivity
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STABILITY
Stable: Stable.
Materials to Avoid: Strong oxidizing agents.
HAZARDOUS DECOMPOSITION PRODUCTS
Hazardous Decomposition Products: Carbon monoxide, Carbon dioxide,
Hydrogen chloride gas.
HAZARDOUS POLYMERIZATION
Hazardous Polymerization: Will not occur
Section 11 - Toxicological Information
ROUTE OF EXPOSURE
Skin Contact: Causes burns. Vesicant. Causes blisters on contact
with skin.
Skin Absorption: Readily absorbed through skin. Toxic if
absorbed through skin.
Eye Contact: Causes burns.
Inhalation: Material is extremely destructive to the tissue of
the mucous membranes and upper respiratory tract. Toxic if
inhaled.
Ingestion: Toxic if swallowed.
TARGET ORGAN(S) OR SYSTEM(S)
Blood. Cardiovascular system. Central nervous system. Liver.
Kidneys. Spleen. Pancreas.
SIGNS AND SYMPTOMS OF EXPOSURE
Symptoms of exposure may include burning sensation, coughing,
wheezing, laryngitis, shortness of breath, headache, nausea, and
vomiting. Inhalation may result in spasm, inflammation and edema
of the larynxand bronchi, chemical pneumonitis, and pulmonary
edema. Material is extremely destructive to tissue of the mucous
membranes and upper respiratory tract, eyes, and skin.
CHRONIC EXPOSURE - CARCINOGEN
Result: Potential cancer hazard.
Section 12 - Ecological Information
No data available.
Section 13 - Disposal Considerations
APPROPRIATE METHOD OF DISPOSAL OF SUBSTANCE OR PREPARATION
Contact a licensed professional waste disposal service to dispose
of this material. Dissolve or mix the material with a combustible
solvent and burn in a chemical incinerator equipped with an
afterburner and scrubber. Observe all federal, state, and local
environmental regulations.
Section 14 - Transport Information
DOT
Proper Shipping Name: Phenol solutions
UN#: 2821
Class: 6.1
Packing Group: Packing Group II
Hazard Label: Toxic substances.
PIH: Not PIH
SIGMA - P1944 www.sigma-aldrich.com Page 4
IATA
Proper Shipping Name: Phenol solution
IATA UN Number: 2821
Hazard Class: 6.1
Packing Group: II
Section 15 - Regulatory Information
EU ADDITIONAL CLASSIFICATION
Symbol of Danger: T
Indication of Danger: Toxic.
R: 23/24/25-34-40-48/20/21/22-68
Risk Statements: Toxic by inhalation, in contact with skin and
if swallowed. Causes burns. Limited evidence of a carcinogenic
effect. Harmful: danger of serious damage to health by prolonged
exposure through inhalation in contact with skin, and if
swallowed. Possible risk of irreversible effects.
S: 26-36/37/39-45
Safety Statements: In case of contact with eyes, rinse
immediately with plenty of water and seek medical advice. Wear
suitable protective clothing, gloves, and eye/face protection.
In case of accident or if you feel unwell, seek medical advice
immediately (show the label where possible).
US CLASSIFICATION AND LABEL TEXT
Indication of Danger: Toxic.
Risk Statements: Toxic by inhalation, in contact with skin and
if swallowed. Causes burns. Limited evidence of a carcinogenic
effect. Harmful: danger of serious damage to health by prolonged
exposure through inhalation in contact with skin, and if
swallowed. Possible risk of irreversible effects.
Safety Statements: In case of contact with eyes, rinse
immediately with plenty of water and seek medical advice. Wear
suitable protective clothing, gloves, and eye/face protection.
In case of accident or if you feel unwell, seek medical advice
immediately (show the label where possible).
US Statements: Readily absorbed through skin. Vesicant.
Combustible. Target organ(s): Central nervous system. Liver.
Calif. Prop. 65 carcinogen.
UNITED STATES REGULATORY INFORMATION
SARA LISTED: No
CANADA REGULATORY INFORMATION
WHMIS Classification: This product has been classified in
accordance with the hazard criteria of the CPR, and the MSDS
contains all the information required by the CPR.
DSL: No
NDSL: No
Section 16 - Other Information
DISCLAIMER
For R&D use only. Not for drug, household or other uses.
WARRANTY
The above information is believed to be correct but does not
purport to be all inclusive and shall be used only as a guide. The
information in this document is based on the present state of our
knowledge and is applicable to the product with regard to
appropriate safety precautions. It does not represent any
SIGMA - P1944 www.sigma-aldrich.com Page 5
guarantee of the properties of the product. Sigma-Aldrich Inc.,
shall not be held liable for any damage resulting from handling or
from contact with the above product. See reverse side of invoice
or packing slip for additional terms and conditions of sale.
Copyright 2008 Sigma-Aldrich Co. License granted to make unlimited
paper copies for internal use only.
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