Revision Date: 5/30/2006
MATERIAL SAFETY DATA SHEET
Conforms to 93/112/EC and ISO 11014-1
1. CHEMICAL PRODUCT AND COMPANY IDENTIFICATION
PRODUCT NAME: PRODUCT NUMBER: EC-862
TE Buffer 100X
CHEMICAL NAMES/
DESCRIPTION:
MANUFACTURER: National Diagnostics, Inc. TELEPHONE NUMBER:
305 Patton Drive (800) 526-3867
Atlanta, GA 30336 (404) 699-2121
EMERGENCY NUMBER:
CHEMTREC (800) 424-9300
2. COMPOSITION / INFORMATION ON INGREDIENTS
Component % Comp CAS # EINECS # TLV (units)
ETHYLENEDIAMINETETRAACETIC ACID (EDTA) 4.7 60-00-4 200-449-4 None Established
Water 80 7732-18-5 231-791-2
TRIS HYDROCHLORIDE 15.3 1185-53-1 214-684-5
EEC LABEL SYMBOL AND CLASSIFICATION
R: 36/37/38
Irritating to eyes, respiratory system and skin.
S: 22-26
Do not breath dust. In case of contact with eyes, rinse immediately with
plenty of water and seek medical advice.
3. HAZARDS IDENTIFICATION
APPEARANCE AND ODOR: Clear odorless liquid.
EMERGENCY OVERVIEW - IMMEDIATE HAZARD
ETHYLENEDIAMINETETRAACETIC ACID (EDTA)
CAUTION! MAY BE HARMFUL IF SWALLOWED. MAY CAUSE IRRITATION TO SKIN, EYES, AND RESPIRATORY
TRACT.
Water
No information found.
TRIS HYDROCHLORIDE
WARNING! HARMFUL IF SWALLOWED. MAY CAUSE IRRITATION TO SKIN, EYES, AND RESPIRATORY TRACT.
EMERGENCY OVERVIEW - CHRONIC HAZARD WARNING
ETHYLENEDIAMINETETRAACETIC ACID (EDTA)
No information found.
Water
No information found.
TRIS HYDROCHLORIDE
No information found.
POTENTIAL HEALTH EFFECTS
INHALATION
ETHYLENEDIAMINETETRAACETIC ACID (EDTA)
Causes irritation of the mucous membrane and upper respiratory tract.
Water
No information found.
TRIS HYDROCHLORIDE
May cause respiratory tract irriation. The toxigological properties of this substance have not been fully
investigated.
INGESTION
ETHYLENEDIAMINETETRAACETIC ACID (EDTA)
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ETHYLENEDIAMINETETRAACETIC ACID (EDTA)
May cause gastrointestinal irritation with nausea, vomiting and diarrhea. Exposure may cause kidney injury,
muscle cramps, bone-marrow depression, and a generalized allergic reaction.
Water
No information found.
TRIS HYDROCHLORIDE
May cause gastrointestinal irritation with nausea, vomiting and diarrhea. May cause severe gastrointestinal
tract irritation with nausea, vomiting and possible burns. The toxicological properties of this substance have
not been fully investigated.
SKIN
ETHYLENEDIAMINETETRAACETIC ACID (EDTA)
Causes skin irritation. Causes redness and pain.
Water
No information found.
TRIS HYDROCHLORIDE
Causes skin irritation. Causes redness and pain.
EYES
ETHYLENEDIAMINETETRAACETIC ACID (EDTA)
Causes eye irritation. Causes redness and pain.
Water
No information found.
TRIS HYDROCHLORIDE
Causes eye irritation. Causes redness and pain.
SIGNS AND SYMPTOMS 0F OVEREXPOSURE
INHALATION
ETHYLENEDIAMINETETRAACETIC ACID (EDTA)
Symptoms may include coughing and shortness of breath.
Water
No information found.
TRIS HYDROCHLORIDE
Symptoms may include coughing and shortness of breath.
INGESTION
ETHYLENEDIAMINETETRAACETIC ACID (EDTA)
Symptoms may include irritation and reddening to the mucous membranes of the mouth, esophagus, and
gastrointestinal tract.
Water
No information found.
TRIS HYDROCHLORIDE
Symptoms may include irritation and reddening to the mucous membranes of the mouth, exophagus, and
gastrointestinal tract.
SKIN
ETHYLENEDIAMINETETRAACETIC ACID (EDTA)
Symptoms may include irritation with redness and pain.
Water
No information found.
TRIS HYDROCHLORIDE
Symptoms may include irritation with redness and pain.
EYES
ETHYLENEDIAMINETETRAACETIC ACID (EDTA)
Symptoms may include irritation, redness, pain, and corneal damage.
Water
No information found.
TRIS HYDROCHLORIDE
Symptoms may include irritation, redness, pain, and corneal damage.
CARCINOGENICITY
ETHYLENEDIAMINETETRAACETIC ACID (EDTA)
Not listed by NTP or IARC as a known or anticipated carcinogen.
Water
No information available.
TRIS HYDROCHLORIDE
Not listed by OSHA, ACGIH, NIOSH, NTP or IARC as a known or anticipated carcinogen.
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MUTAGENICITY
ETHYLENEDIAMINETETRAACETIC ACID (EDTA)
Cytogenetic Analysis: intraperitoneal - mouse 50mmol/L. DNA Inhibition: hamster fibroblast 500ug/L, rabbit
kidney 250 umol/L.
Water
No information available.
TRIS HYDROCHLORIDE
No information available.
REPRODUCTIVE TOXICITY
ETHYLENEDIAMINETETRAACETIC ACID (EDTA)
Fertility: Post-implantation mortality, oral - rat TDLo = 7632 mg/kg.
Water
No information available.
TRIS HYDROCHLORIDE
No information available.
TERATOGENIC EFFECTS
ETHYLENEDIAMINETETRAACETIC ACID (EDTA)
Embryo or Fetus: Stunted fetus, oral - rat TDLo = 7632 mg/kg. Specific developmental abnormalities:
cardiovascular, craniofacial, musculoskeletal, respiratory, and urogenital, oral - rat TDLo = 7632 mg/kg.
Water
No information available.
TRIS HYDROCHLORIDE
No information available.
ROUTES OF ENTRY
ETHYLENEDIAMINETETRAACETIC ACID (EDTA)
Inhalation, ingestion or skin contact.
Water
No information available
TRIS HYDROCHLORIDE
Inhalation, ingestion or skin contact.
TARGET ORGAN STATEMENT
ETHYLENEDIAMINETETRAACETIC ACID (EDTA)
No information available
Water
No information available
TRIS HYDROCHLORIDE
No information available
4. FIRST AID MEASURES
INHALATION:
Remove to fresh air. If not breathing, give artificial respiration. If breathing is difficult, give oxygen. Call a physician.
INGESTION:
If swallowed, wash out mouth with water provided person is conscious. Call a physician.
SKIN:
Immediately flush skin with plenty of soap and water for at least 15 minutes while removing contaminated clothing
and shoes. Get medical attention. Wash clothing before reuse. Thoroughly clean shoes before reuse.
EYES:
Immediately flush eyes with plenty of water for at least fifteen minutes, lifting lower and upper eyelids occasionally.
Get medical attention immediately.
5. FIRE FIGHTING MEASURES
FLASH POINT: N.A. FLAMMABLE LIMITS: N.A.
FLASH POINT METHOD: N.A. AUTOIGNITION TEMPERATURE: N.A.
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EXTINGUISHING MEDIA
Water spray, dry chemical, alcohol-resistant foam, or carbon dioxide.
PROTECTIVE EQUIPMENT
In the event of a fire, wear full protective clothing and NIOSH-approved self-contained breathing apparatus with full
facepiece operated in the pressure demand or other positive pressure mode.
HAZARDOUS COMBUSTION PRODUCTS:
N.A.
UNUSUAL FIRE AND EXPLOSION HAZARDS
Not considered an explosion hazard.
NFPA CODES: Health: 1 Flammability: 0 Reactivity: 0
6. ACCIDENTAL RELEASE MEASURES
STEPS TO BE TAKEN IN CASE MATERIAL IS RELEASED OR SPILLED
Use a vacuum cleaner equipped with charcoal exhaust scrubber or hose from mechanical exhaust ventilation
system to vacuum spills. If this is not possible, sweep up spills and place in a covered waste disposal container.
Flush area with water.
WASTE DISPOSAL METHOD
Disposal must be made in accordance with applicable federal, state, and local regulations.
PERSONAL PRECAUTIONS
Wear appropriate protective equipment as specified in section 8.
7. HANDLING AND STORAGE
HANDLING
Avoid contact and inhalation. Do not get in eyes, on skin, on clothing. Wash thoroughly after handling.
STORAGE
Keep in a tightly closed container, stored in a cooled, dry, ventilated area. Protect from physical damage. Isolate
from incompatible materials (section 10).
STORAGE TEMPERATURE: 20C
DISPOSAL
Observe all national, state, and local regulations regarding disposal.
8. EXPOSURE CONTROLS / PERSONAL PROTECTION
AIRBORNE EXPOSURE LIMITS:
Component: ETHYLENEDIAMINETETRAACETIC ACID (EDTA)
ACGIH Threshold Limit Value (TLV): None Established
OSHA Permissible Exposure Limit (PEL): None Established
Component: Water
ACGIH Threshold Limit Value (TLV):
OSHA Permissible Exposure Limit (PEL):
Component: TRIS HYDROCHLORIDE
ACGIH Threshold Limit Value (TLV):
OSHA Permissible Exposure Limit (PEL):
ENGINEERING CONTROLS
A system of local and/or general exhaust is recommended to keep employee exposures below the Airborn Exposure
Limits. Local exhaust ventilation is generally preferred because it can control the emissions of the contaminant at its
source.
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RESPIRATORY PROTECTION
For conditions of use where exposure to the dust or mist is apparent, a full-face dust/mist respirator may be worn.
For emergencies or instances where the exposure levels are not known, use a full-face positive-pressure, air-
supplied respirator.
EYE PROTECTION
Safety glasses.
SKIN PROTECTION
Wear protective gloves and clean body covering clothing.
OTHER CONTROL MEASURES
9. PHYSICAL PROPERTIES
Boiling Point as water Evaporation Rate water
Melting Point ~6C Solubility in Water miscible
Vapor Pressure water pH 9
mm Hg
Vapor Density Air Specific Gravity 1.12
Air = 1 (H20 = 1)
% Volatile by Volume 80
10. STABILITY AND REACTIVITY
STABILITY
Stable at room temperature in closed containers under normal storage and handling conditions. Decarboxylates
above 150C.
CONDITIONS TO AVOID
Incompatible materials, dust generation, excess heat.
HAZARDOUS DECOMPOSITION PRODUCTS
Nitrogen oxides, carbon monoxide, irritating and toxic fumes and gases, carbon dioxide.
HAZARDOUS POLYMERIZATION
Will not occur
INCOMPATIBLES
ETHYLENEDIAMINETETRAACETIC ACID (EDTA)
Strong oxidizing agents, strong bases, aluminum, copper, copper alloys, nickel.
Water
No incompatibility data found.
TRIS HYDROCHLORIDE
Bases, oxidizing agents, acids, aldehydes, copper, brass, and aluminum.
11. TOXICOLOGICAL INFORMATION
PRODUCT LD50 VALUES
TE Buffer 100X Oral Rat LD50 (mg/kg): 12760
TE Buffer 100X Dermal Rabbit LD50 (mg/kg): N.D.
COMPONENT CANCER LIST STATUS
NTP Carcinogen
Component Known Anticipated IARC Category
ETHYLENEDIAMINETETRAACETIC ACID (EDTA) No No None
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ETHYLENEDIAMINETETRAACETIC ACID (EDTA) No No None
Water No No None
TRIS HYDROCHLORIDE No No None
12. ECOLOGICAL INFORMATION
ETHYLENEDIAMINETETRAACETIC ACID (EDTA)
EDTA is expected to complex with trace metals and alkaline earth metals present in the soil, thereby causing an increase
in the total solubility of the metals. EDTA may eventually predominate as the solubility of the metals. EDTA may
eventually predominate as the Fe(III) chelate in acidic soils and as the Ca chelate in alkaline soils. Biodegradation of
EDTA in aerobic soils is the dominant.
Water
Not applicable.
TRIS HYDROCHLORIDE
No information found.
13. DISPOSAL CONSIDERATIONS
Observe all national, state, and local regulations regarding disposal.
14. TRANSPORT INFORMATION
D.O.T.
Proper Shipping Name: Not Regulated.
Hazard Class: N.A.
UN Number: N.A.
Packing Group: N.A.
I.A.T.A.
Proper Shipping Name: Not Regulated.
Hazard Class: N.A.
UN Number: N.A.
Packing Group: N.A.
I.M.O.
Proper Shipping Name: Not Regulated.
Hazard Class: N.A.
UN Number: N.A.
Packing Group: N.A.
15. REGULATORY INFORMATION
UNITED STATES
TSCA Regulatory:
All intentional ingredients are listed on the TSCA Inventory.
SARA 311/312 Hazard Categories
Component Fire Pressure Reactivity Acute Chronic
ETHYLENEDIAMINETETRAACETIC ACID (EDTA) No No No Yes No
Water No No No No No
TRIS HYDROCHLORIDE No No No Yes No
EUROPE
EEC Regulatory:
All intentional ingredients are listed on the European EINECS Inventory.
EEC LABEL SYMBOL AND CLASSIFICATION
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EEC LABEL SYMBOL AND CLASSIFICATION
R: 36/37/38
Irritating to eyes, respiratory system and skin.
S: 22-26
Do not breath dust. In case of contact with eyes, rinse immediately with
plenty of water and seek medical advice.
16. OTHER INFORMATION
NFPA CODES: Health: 1 Flammability: 0 Reactivity: 0
MANUFACTURER DISCLAIMER: The information given herein is offered in good faith as accurate, but without
guarantee. Conditions of use and suitability of the product for particular uses are beyond our control. All risks of use of
the product are therefore assumed by the user. Nothing is intended as a recommendation for uses which infringe valid
patents or as extending license under valid patents. Appropriate warnings and safe handling procedures should be
provided to handlers and users.
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