MATERIAL SAFETY DATA SHEET
Effective Date: 2/26/08 Document: F11-557-5-E Supersedes: F11-01-083-02-C Page 1 of 1
Propidium Iodide at 250 ug/mL
Catalog #: 557, 638
Synonyms: PI
Chemical CAS # of Active Reagent: 25535-16-4
Chemical Name of Active Reagent: Phenanthridinium, 3,8-diamino-5-[3-(diethylmethylammonio) propyl]-6-phenyl,-diiodide
Identification
Molecular Weight of Active Reagent: 668.4
Chemical Formula of Active Reagent: C27H34I2N4
Contains propidium iodide (PI) staining reagent at 250 碌g/ml in DI H2O. PI exhibits mutagenic properties as demonstrated in Salmonella
Hazards typhimurium using 200 ug/plate and at 25 碌M/L with Saccharomyces cerevisiae. PI is not currently listed as a carcinogen by NTP IARC or OSHA
,
Identification but should be treated as such. Symptoms of exposure are undefined but this staining reagent should always be handled using protective gloves,
clothing, and eyewear. It was determined to have a LD50 level of 16 mg/kg when administered subcutaneously in mice.
PI stain is a clear, light pink, odorless solution.
Boiling point, C: 100
Vapor pressure (mm Hg): No data available.
Vapor density: No data available.
Characteristics
Solubility in water: Soluble at room temperature.
Specific gravity: Not applicable.
% volatile by volume: Not applicable.
Evaporation rate: No data available.
pH: 4.5 - 6.0
Propidium iodide stain, as supplied by ICT, is stable for 18 months at 2 - 8潞C when stored in amber vials and protected from
Storage
light. Always store protected from light in a tightly closed vial. Vials may be hazardous when empty since they contain product
and Stability
residues. Do not breathe vapor or mist.
Health
Propidium iodide may enter the body through inhalation, ingestion, and skin and eye contact.
Hazard
Personal protective equipment, such as lab coat, safety glasses with side shields, gloves should be worn at all times while han-
Safety Control
dling this product. Maintain eye wash fountain and safety shower in work area. Safe laboratory practices should be followed.
Measures
Always wash thoroughly after handling. Never take internally. Do not breathe vapor or mist.
Exposure
No exposure guidelines are available.
Guidelines
Propidium iodide could be a potential carcinogen; avoid any contact with skin, eyes, or take internally. Carefully remove and
dispose of gloves after use. Remove contaminated clothing and shoes; wash before reuse.
First Aid Skin: Wash thoroughly with plenty of soap and water for at least 15 minutes. Remove contaminated
Measures clothing and shoes. Launder contaminated clothing. Throw away contaminated shoes.
Eyes: Immediately flush with plenty of water for at least 15 minutes.
Ingestion: If solution is swallowed, seek medical advice.
Inhalation: Remove to fresh air, seek medical advice.
Fire/Explosion
The 250 碌g/ml solution of propidium iodide stain is not a potential fire or explosion hazard.
Hazard
For simple counter top spills, use protective gloves and clothing when promptly cleaning up spills. Spilled solutions may be adsorbed into paper tow-
eling and disposed of according to local waste management ordinances. The water-soluble characteristics of this reagent allow the area to be
Accidental cleaned up using soap and water. For large spills, evacuate area of all unnecessary employees. Wear appropriate personal protective equipment
Release Measures including: neoprene (or equivalent) gloves, safety glasses with side shields, and lab coat. Contain the spill and dispose of in compliance with federal,
state, and local regulations. US regulations (CERCLA) require reporting spills and releases to soil, water, and air in excess of reportable quantities.
Toll free number to US Coast Guard National Response Center is (800) 424-8802.
EMERGENCY # FOR NATIONAL RESPONSE CENTER (800) 424-8802
Immunochemistry TECHNOLOGIES, LLC
9401 James Ave. S. #155, Bloomington, MN 55431 USA tel 952-888-8788 fax 952-888-8988
www.immunochemistry.com 800-829-3194
THE ABOVE INFORMATION IS BELIEVED TO BE CORRECT BUT IS NOT NECESSARILY ALL-INCLUSIVE AND
SHOULD BE USED ONLY AS A GUIDE. IMMUNOCHEMISTRY TECHNOLOGIES, LLC SHALL NOT BE HELD LIABLE
FOR ANY DAMAGE RESULTING FROM HANDLING OR FROM CONTACT WITH THE ABOVE PRODUCT. INDIVIDUALS
RECEIVING THE INFORMATION MUST EXERCISE THEIR INDEPENDENT JUDGMENT IN DETERMINING ITS
APPROPRIATENESS FOR A PARTICULAR PURPOSE.
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