TURBO DNA-free鈩?
AMBION, INC.
Catalog #1907
TURBO DNase
Material Safety Data Sheet
Section 1-Identification of Product
2238G
Health: 1 Fire: 1 Stability: 0
WHMIS Classification
Contact Information
Ambion, Inc 2130 Woodward St. Austin, TX 78744-1832 Tel: +1 512 651 0200 US Toll-free Tel: 800 888 8804 E-
mail: techserv@ambion.com Web address www.ambion.com
Ambion (Europe) LTD Huntingdon, Cambidgeshire UK PE29 6XY Tel:+44 (0)1480 373 020 Fax: +44 (0)1480 373
010 E-mail: eurotech@ambion.com Web address: www.ambion.com
Emergency Contact: In Europe, call 112. In USA, call 911
Section 2-Composition/Information
Hazardous Ingredients (Specific) % CAS # LD50 LC50
Glycerol 50 56-81-5 Oral-Rat Inhalation, rat:
LD50: 12,600 >570 mg/m3/1H
mg/kg
Section 3-Hazard Identification
Routes of Entry
Skin Contact Causes skin irritation
Skin Absorption Causes skin irritation
Eye Contact Causes eye irritation
Inhalation Causes respiratory irritation
Ingestion Causes gastrointestinal irritation
[Emergency Overview]
Caution! May cause eye and skin irritation. May cause respiratory and digestive tract irritation. This is expected to be
a low hazard for usual industrial handling.
WHMIS Symbols
N.A.
[Potential Health Effects]
Poison by subcutaneous route. Mildly toxic by ingestion. Human systemic effects by ingestion: headache and nausea
or vomiting. Experimental reproductive effects. Human mutation data reported. A skin and eye irritant. In the form of
mist it is a nuisance particulate and inhalation irritant. Combustible liquid when exposed to heat, flame, or powerful
oxidizers. Mixtures with hydrogen peroxide are highly explosive. Ignites on contact with potassium permanganate,
calcium hypochlorite. Mixture with nitric acid + sulfuric acid forms the explosive glyceryl nitrate. Mixture with
perchloric acid + lead oxide forms explosive perchlorate esters. Confined mixture with chlorine explodes if heated to
70鈥?80潞. Can react violently with acetic anhydride, aniline + nitrobenzene, Ca(OCl)2, CrO3, Cr2O3, F2 + PbO,
phosphorus triiodide, ethylene oxide + heat, KMnO4, K2O2, AgClO4, Na2O2, NaH. Energetic reaction with sodium
hydride. Mixture with nitric acid + hydrofluoric acid is a storage hazard due to gas evolution. To fight fire, use
alcohol foam, CO2, dry chemical. When heated to decomposition it emits acrid smoke and fumes.
Section 4-First Aid Measures
Skin Contact Flush skin with plenty of water for at least 15 minutes while removing contaminated clothing
and shoes. Get medical aid if irritation develops or persists. Wash clothing before reuse.
Eye Contact Flush eyes with plenty of water for at least 15 minutes, occasionally lifting the upper and lower
eyelids. If irritation develops, get medical aid.
Inhalation Remove from exposure and move to fresh air immediately. If not breathing, give artificial
respiration. If breathing is difficult, give oxygen. Get medical aid if cough or other symptoms
appear.
Ingestion Never give anything by mouth to an unconscious person. Do NOT induce vomiting. If conscious
and alert, rinse mouth and drink 2-4 cupfuls of milk or water. Get medical aid if irritation or
symptoms occur.
Section 5-Fire Fighting Measures
Flammable NA
Means of Extinction Use water spray to cool fire-exposed containers. Use agent
most appropriate to extinguish fire. Use water spray, dry
chemical, carbon dioxide, or appropriate foam.
Flashpoint (oC) and Method NA
Upper Flammable Limit (% by volume) NA
Lower Flammable Limit (% by volume) NA
Version 1 Page 1 of 3 Printed: 12/27/2004
Created:111904 QC Form:2068
Revision Date: NA
TURBO DNA-free鈩?
AMBION, INC.
Catalog #1907
TURBO DNase
Material Safety Data Sheet
Autoignition Temperature (oC) NA
Explosion Data 鈥? Sensitivity to Impact NA
Explosion Data 鈥? Sensitivity to Static Discharge NA
Hazardous Combustion Products Carbon monoxide, irritating and toxic fumes and gases,
carbon dioxide.
NFPA Health: 1 Fire: 1 Stability: 0
Section 6-Accidental Release Measures
Leak and Spill Procedures
Clean up spills immediately, observing precautions in the Protective Equipment section. Sweep up or absorb material,
then place into a suitable clean, dry, closed container for disposal.
Section 7-Handling and Storage
Handling Procedures and Wash thoroughly after handling. Wash hands before eating. Use with
Equipment adequate ventilation. Avoid contact with eyes, skin, and clothing. Keep
container tightly closed. Avoid ingestion and inhalation. Wash
clothing before reuse
Storage Requirements Keep in a tightly closed container, stored in a cool, dry, ventilated area. Protect
against physical damage. Isolate from incompatible substances. Containers of
this material may be hazardous when empty since they retain product residues
(dust, solids); observe all warnings and precautions listed for the product.
Section 8-Exposure Control/Personal Protection
Exposure Limits
ACGIH TLV 10 mg/m3 TWA
OSHA PEL 15 mg/m3 TWA (total dust); 5 mg/m3 TWA (respirable fraction)
Other (specify) N.A.
Engineering Controls (specific)
General Facilities storing or utilizing this material should be equipped with an eyewash
facility and a safety shower. Use adequate ventilation to keep airborne
concentrations low
Local Exhaust A system of local and/or general exhaust is recommended to keep employee
exposures as low as possible. Local exhaust ventilation is generally preferred
because it can control the emissions of the contaminant at its source, preventing
dispersion of it into the general work area. Please refer to the ACGIH document,
Industrial Ventilation, A Manual of Recommended Practices, most recent
edition, for details
Other N.A.
Personal Protective Equipment (specific)
Gloves Synthetic Gloves
Respirator A respiratory protection program that meets OSHA's 29 CFR 1910.134 and
ANSI Z88.2 requirements or European Standard EN 149 must be followed
whenever workplace conditions warrant a respirator's use.
Eye Wear appropriate protective eyeglasses or chemical safety goggles as described
by OSHA's eye and face protection regulations in 29 CFR 1910.133 or European
Standard EN166. Maintain eye wash fountain and quick-drench facilities in work
area
Footwear Closed toe shoes
Clothing Wear appropriate protective clothing to prevent skin exposure.
Other N.A.
Section 9-Physical and Chemical Properties
Physical State Liquid
Odor and Appearance Clear
Odor Threshold (ppm) Not Available
Specific Gravity Not Available
Vapor Density (Air=1) Not Available
Vapor Pressure (mmHg) Not Available
Evaporation Rate Not Available
Boiling Point (oC) Not Available
Freezing Point (oC) Not Available
pH Not Available
Coefficient of Water/Oil Distribution Not Available
Version 1 Page 2 of 3 Printed: 12/27/2004
Created:111904 QC Form:2068
Revision Date: NA
TURBO DNA-free鈩?
AMBION, INC.
Catalog #1907
TURBO DNase
Material Safety Data Sheet
[Solubility in Water] Not Available
Section 10-Stability and Reactivity
Chemical Stability Stable
Incompatible with other substances Acetic anhydride, potassium permanganate, strong acids, caustics
(e.g. ammonia, ammonium hydroxide, calcium hydroxide,
potassium hydroxide, sodium hydroxide), isocyanates, oxidizing
agents, aliphatic amines
Reactivity Not Available
Hazardous Decomposition Products Carbon monoxide, irritating and toxic fumes and gases, carbon
dioxide.
Section 11-Toxicological Information
Acute Effects Not Tested
Chronic Effects Not Tested
Irritancy of Product Eyes, skin, respiratory, gastrointestinal
Skin Sensitization Not Tested
Respiratory Sensitization Not Tested
Carcinogenicity
IARC (1,2A, or 2B) Not Listed
ACGIH (A1, A2, or A3) Not Listed
Reproductive Toxicity Not Listed
Teratogenicity Not Listed
Embryotoxicity Not Listed
Mutagenicity Not Listed
Name of Synergistic Not Listed
Products/Effects
Section 12-Ecological Information
Aquatic Toxicity LC50 (96 Hr.) rainbow trout = 50-67 mg/L; 12 degrees CLC50 (96 Hr.) goldfish
= >5000 mg/L
Section 13-Disposal Considerations
Waste Disposal Chemical waste generators must determine whether a discarded chemical is
classified as a hazardous waste. US EPA guidelines for the classification
determination are listed in 40 CFR Parts 261.3. Additionally, waste generators
must consult state and local hazardous waste regulations to ensure complete and
accurate classification.
Section 14-Transport Information
Special Shipping Information
PIN Not Listed
TDG Not Listed
[DOT] Not Listed
[IMO] Not Listed
[ICAO] Not Listed
Section 15-Regulatory Information
[WHMIS Classification] Not Listed
[OSHA] Not Listed
[SARA] CAS # 56-81-5: chronic
[TSCA] Listed
Section 16-Other Information
This bulletin is for your guidance and is based upon information and tests believed to be reliable. Ambion makes no
guarantee of the accuracy or completeness of the data and shall not be liable for any damages thereto. The data are
offered solely for your consideration, investigation, and verification. These suggestions should not be confused with
state, municipal, or insurance requirements, or with national safety codes and constitute no warranty. Any use of
these data and information must be determined by the user to be in accordance with applicable federal, state, and local
regulations.
Version 1 Page 3 of 3 Printed: 12/27/2004
Created:111904 QC Form:2068
Revision Date: NA
SAFETY STATEMENT
This document is only available in English.
1. Identification of the substance/preparation and company/undertaking
Product/Kit Name: TURBO DNA-free鈩?
Catalog #: 1907
Product/Component Name: DNase Inactivation Reagent
Catalog Number: 8174G
Supplier: Ambion, Inc.
Emergency Telephone Numbers:
USA: 1-800-888-8804
Europe: +44 (0) 1480 373 020
2. Composition/ information on ingredients
Substance/Preparation:
To the present knowledge of Ambion, Inc., this product does not contain any hazardous
ingredients in quantities requiring reporting in this section, in accordance with EU
regulations or National regulations.
To Ambion鈥檚 knowledge this substance/preparation is not classified as hazardous
according to EU, US, or any other known national regulations.
9. Physical and chemical properties
Physical State: Liquid
Color: Colorless
16. Other information:
To the best of Ambion鈥檚 knowledge, the information contained herein is accurate.
However, Ambion, Inc. does not assume any liability whatsoever for the accuracy or
completeness of the information contained herein.
Final determination of suitability of any material is the sole responsibility of the user. All
materials may present unknown hazards and should be used with caution.
This is not an MSDS. According to EU and US regulations we are not required to supply
an MSDS for a product, which is not classified as hazardous.
Version# 1
Revision Date: N.A. Page 1 of 1 Printed:12/27/2004
Created Date:111904 QC Form 2066
TURBO DNA-free鈩?
AMBION, INC.
Catalog #1907
10X TURBO DNase Buffer
Material Safety Data Sheet
Section 1-Identification of Product
8167G
WHMIS Classification Health: 2 Fire: 1 Stability: 1
Contact Information
Ambion, Inc 2130 Woodward St. Austin, TX 78744-1832 Tel: +1 512 651 0200 US Toll-free Tel: 800 888 8804 E-
mail: techserv@ambion.com Web address www.ambion.com
Ambion (Europe) LTD Huntingdon, Cambidgeshire UK PE29 6XY Tel:+44 (0)1480 373 020 Fax: +44 (0)1480 373
010 E-mail: eurotech@ambion.com Web address: www.ambion.com
Emergency Contact: In Europe, call 112. In USA, call 911
Section 2-Composition/Information
Hazardous Ingredients (Specific) % CAS # LD50 LC50
Sodium Chloride ~3 7647-14-5 Oral-Rat No information
LD50: 2600
mg/kg
Tris ~1.5 77-86-1 Oral-Rat No information
LD50: 5900
mg/kg
Section 3-Hazard Identification
Routes of Entry
Skin Contact Causes skin irritation
Skin Absorption Causes skin irritation
Eye Contact Causes eye irritation
Inhalation Causes respiratory irritation
Ingestion Sodium Chloride - Very large doses can cause vomiting, diarrhea,
and prostration. Dehydration and congestion occur in most internal
organs. Hypertonic salt solutions can produce violent inflammatory
reactions in the gastrointestinal tract. May cause severe
gastrointestinal tract irritation with nausea, vomiting and possible
burns.
[Emergency Overview]
N.A>
WHMIS Symbols
N.A.
[Potential Health Effects]
Sodium Chloride -Poison by intraperitoneal and intracervical routes. Moderately toxic by ingestion,
intravenous, and subcutaneous routes. An experimental teratogen. Human systemic effects by ingestion:
blood pressure increase. Human reproductive effects by intraplacental route: terminates pregnancy.
Experimental reproductive effects. Human mutation data reported. A skin and eye irritant. When bulk
sodium chloride is heated to high temperature, a vapor is emitted that is irritating, particularly to the eyes.
Ingestion of large amounts of sodium chloride can cause irritation of the stomach. Improper use of salt
tablets may produce this effect. Potentially explosive reaction with dichloromaleic anhydride + urea.
Electrolysis of mixtures with nitrogen compounds may form the explosive nitrogen trichloride. Reaction
with burning lithium forms the dangerously reactive sodium. The molten salt at 1100潞 reacts explosively
with water. Violent reaction with BrF3. When heated to decomposition it emits toxic fumes of Cl炉 and
Na2O
Tris - Moderately toxic by ingestion and intravenous routes. When heated to decomposition it emits toxic
fumes of NOx
Skin Contact Get medical aid. Flush skin with plenty of water for at least 15 minutes while removing
contaminated clothing and shoes
Eye Contact Flush eyes with plenty of water for at least 15 minutes, occasionally lifting the upper and lower
eyelids. Get medical aid immediately
Inhalation Get medical aid immediately. Remove from exposure and move to fresh air immediately. If not
breathing, give artificial respiration. If breathing is difficult, give oxygen.
Version 1 Page 1 of 4 Printed: 12/27/2004
Created:111904 QC Form:2067
Revision Date: NA
TURBO DNA-free鈩?
AMBION, INC.
Catalog #1907
10X TURBO DNase Buffer
Material Safety Data Sheet
Ingestion Never give anything by mouth to an unconscious person. Do NOT induce vomiting. If conscious
and alert, rinse mouth and drink 2-4 cupfuls of milk or water. Get medical aid if irritation or
symptoms occur.
Section 5-Fire Fighting Measures
Flammable Slightly flammable
CO2, H2O, Foam, Dry Chemical
Means of Extinction
Flashpoint (oC) and Method Not Tested
Upper Flammable Limit (% by volume) Not Tested
Lower Flammable Limit (% by volume) Not Tested
Autoignition Temperature (oC) Not Tested
Explosion Data 鈥? Sensitivity to Impact Not Tested
Explosion Data 鈥? Sensitivity to Static Discharge Not Tested
Hazardous Combustion Products Sodium Chloride - When heated to above 801C (1474F) it
emits toxic fumes of chloride and sodium oxide.
NFPA Health: 2 Fire: 1 Stability: 1
Section 6-Accidental Release Measures
Leak and Spill Procedures
Clean up spills immediately, observing precautions in the Protective Equipment section. Sweep up or absorb material,
then place into a suitable clean, dry, closed container for disposal.
Section 7-Handling and Storage
Handling Procedures and Wash thoroughly after handling. Wash hands before eating. Use with adequate
Equipment ventilation. Avoid contact with eyes, skin, and clothing. Keep container tightly
closed. Avoid ingestion and inhalation. Wash clothing before reuse.
Storage Requirements Keep in a tightly closed container, stored in a cool, dry, ventilated area. Protect
against physical damage. Isolate from incompatible substances. Containers of
this material may be hazardous when empty since they retain product residues
(dust, solids); observe all warnings and precautions listed for the product.
Section 8-Exposure Control/Personal Protection
Exposure Limits
ACGIH TLV Not Listed
OSHA PEL Not Listed
Other (specify) N.A.
Engineering Controls (specific)
General Facilities storing or utilizing this material should be equipped with an eyewash
facility and a safety shower. Use adequate ventilation to keep airborne
concentrations low.
Local Exhaust A system of local and/or general exhaust is recommended to keep employee
exposures as low as possible. Local exhaust ventilation is generally preferred
because it can control the emissions of the contaminant at its source, preventing
dispersion of it into the general work area. Please refer to the ACGIH document,
Industrial Ventilation, A Manual of Recommended Practices, most recent
edition, for details.
Other N.A.
Personal Protective Equipment (specific)
Gloves Synthetic Gloves
Respirator A respiratory protection program that meets OSHA's 29 CFR 1910.134 and
ANSI Z88.2 requirements or European Standard EN 149 must be followed
whenever workplace conditions warrant a respirator's use.
Eye Wear appropriate protective eyeglasses or chemical safety goggles as described
by OSHA's eye and face protection regulations in 29 CFR 1910.133 or European
Standard EN166. Maintain eye wash fountain and quick-drench facilities in work
area
Footwear Closed toe shoes
Clothing Wear appropriate protective clothing to prevent skin exposure
Other N.A.
Section 9-Physical and Chemical Properties
Physical State Liquid
Odor and Appearance Odorless / Colorless
Odor Threshold (ppm) Not Tested
Version 1 Page 2 of 4 Printed: 12/27/2004
Created:111904 QC Form:2067
Revision Date: NA
TURBO DNA-free鈩?
AMBION, INC.
Catalog #1907
10X TURBO DNase Buffer
Material Safety Data Sheet
Specific Gravity Not Tested
Vapor Density (Air=1) Not Tested
Vapor Pressure (mmHg) Not Tested
Evaporation Rate Not Tested
Boiling Point (oC) Not Tested
Freezing Point (oC) Not Tested
pH 8.3
Coefficient of Water/Oil Distribution Not Tested
[Solubility in Water] Not Tested
Section 10-Stability and Reactivity
Chemical Stability Stable
Incompatible with other substances Bromine trifluoride; potassium permanganate plus sulfuric acid.
Copper, brass, aluminum, and oxidizing agents
Reactivity Not Tested
Hazardous Decomposition Products When heated to above 801C (1474F) it emits toxic fumes of
chloride and sodium oxide. Burning may produce carbon
monoxide, carbon dioxide, and Nitrogen oxides. When heated to
temperatures above 300C (572F) it emits toxic fumes of chlorine
gas
Section 11-Toxicological Information
Acute Effects Not Tested
Chronic Effects Not Tested
Irritancy of Product Eyes, skin, respiratory, gastrointestinal
Skin Sensitization Not Tested
Respiratory Sensitization Not Tested
Carcinogenicity
IARC (1,2A, or 2B) Not Listed
ACGIH (A1, A2, or A3) Not Listed
Reproductive Toxicity Not Listed
Teratogenicity Not Listed
Embryotoxicity Not Listed
Mutagenicity Not Listed
Name of Synergistic Not Listed
Products/Effects
Section 12-Ecological Information
Aquatic Toxicity Not Tested
Section 13-Disposal Considerations
Waste Disposal Chemical waste generators must determine whether a discarded chemical is
classified as a hazardous waste. US EPA guidelines for the classification
determination are listed in 40 CFR Parts 261.3. Additionally, waste generators
must consult state and local hazardous waste regulations to ensure complete and
accurate classification.
Section 14-Transport Information
Special Shipping Information
PIN Not Listed
TDG Not Listed
[DOT] Not Listed
[IMO] Not Listed
[ICAO] Not Listed
Section 15-Regulatory Information
[WHMIS Classification] Not Listed
[OSHA] Not Listed
[SARA] 302-no 313-no 311/312 Acute: yes Chronic: no
Tris - 311/312: Acute
[TSCA] Yes
Version 1 Page 3 of 4 Printed: 12/27/2004
Created:111904 QC Form:2067
Revision Date: NA
TURBO DNA-free鈩?
AMBION, INC.
Catalog #1907
10X TURBO DNase Buffer
Material Safety Data Sheet
Section 16-Other Information
This bulletin is for your guidance and is based upon information and tests believed to be reliable. Ambion makes no
guarantee of the accuracy or completeness of the data and shall not be liable for any damages thereto. The data are
offered solely for your consideration, investigation, and verification. These suggestions should not be confused with
state, municipal, or insurance requirements, or with national safety codes and constitute no warranty. Any use of
these data and information must be determined by the user to be in accordance with applicable federal, state, and local
regulations.
Version 1 Page 4 of 4 Printed: 12/27/2004
Created:111904 QC Form:2067
Revision Date: NA
SAFETY STATEMENT
This document is only available in English.
1. Identification of the substance/preparation and company/undertaking
Product/Kit Name: TURBO DNA-free鈩?
Catalog #: 1907
Product/Component Name: Nuclease-free Water
Catalog Number: 9914G
Supplier: Ambion, Inc.
Emergency Telephone Numbers:
USA: 1-800-888-8804
Europe: +44 (0) 1480 373 020
2. Composition/ information on ingredients
Substance/Preparation:
To the present knowledge of Ambion, Inc., this product does not contain any hazardous
ingredients in quantities requiring reporting in this section, in accordance with EU
regulations or National regulations.
To Ambion鈥檚 knowledge this substance/preparation is not classified as hazardous
according to EU, US, or any other known national regulations.
9. Physical and chemical properties
Physical State: Liquid
Color: Colorless
16. Other information:
To the best of Ambion鈥檚 knowledge, the information contained herein is accurate.
However, Ambion, Inc. does not assume any liability whatsoever for the accuracy or
completeness of the information contained herein.
Final determination of suitability of any material is the sole responsibility of the user. All
materials may present unknown hazards and should be used with caution.
This is not an MSDS. According to EU and US regulations we are not required to supply
an MSDS for a product, which is not classified as hazardous.
Version# 1
Revision Date: N.A. Page 1 of 1 Printed:12/27/2004
Created Date:111904 QC Form 2035
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