ANATECH LTD. Page 1 of 7
BRAZILLIANT! Date prepared: March 2005
Date Reviewed: May 2008
MATERIAL SAFETY DATA SHEET
1. CHEMICAL PRODUCT AND COMPANY IDENTIFICATION
Product name: BRAZILLIANT!
Catalog number: 861
General use: Nuclear stain for histology and cytology.
Product description: Aqueous solution of Brazilin.
Manufacturer Emergency contact information
Anatech Ltd. Health: Anatech Ltd. 800-262-8324 8 am - 5 pm ET, M-F
1020 Harts Lake Road Transportation: CHEMTREC 800-424-9300 24 hours
Battle Creek, MI 49037
USA
2. COMPOSITION AND INFORMATION ON INGREDIENTS
Component CAS # Exposure limits
Acetic acid (1.5% v/v) 64-19-7 10 ppm (OSHA, NIOSH, ACGIH 8 hour TWA)
15 ppm (NIOSH, ACGIH 15 minute STEL)
Aluminum ammonium sulfate (9.5%w/v) 17734-24-9 Not established for solutions.
Ethanol (4% v/v) 64-17-5 1000 ppm (OSHA, ACGIH 8 hr TWA)
Brazilin (1% w/v) 474-07-7 Not established.
Sodium iodate (0.02% w/v) 7681-55-2 Not established.
3. HAZARDS IDENTIFICATION
Emergency overview
Red liquid.
Irritant to eyes. Not likely to pose a hazard under normal conditions of use.
Potential health effects
(Human health effects only; animal effects in Section 11: Toxicological Information.)
Primary route(s) of exposure: Eyes and skin.
Inhalation: Inhalation of vapors during normal conditions of use are not likely to present a health hazard.
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BRAZILLIANT! Date prepared: March 2005
Date Reviewed: May 2008
3. HAZARDS IDENTIFICATION (continued)
Eye: Contact of liquid with eyes may cause irritation.
Skin: Brief contact with skin is nonirritating.
Ingestion: Ingestion is likely to produce adverse effects on the gastrointestinal system.
Chronic effects: None expected under anticipated conditions of use.
Signs and symptoms: Affected skin will be stained red. Eyes may water. Effects on the
gastrointestinal tract may include discomfort and nausea.
4. FIRST AID MEASURES
Inhalation: Remove victim to fresh air if coughing or difficulty in breathing is experienced. Consult a physician if
symptoms persist or worsen. Administer oxygen or artificial respiration as needed.
Eye: Flush eyes for at least 15 minutes in an eyewash station. If symptoms persist after washing, consult a
physician.
Skin: Remove contaminated clothing, including footwear; wash before reuse or discard. For minor exposure,
wash affected area with water and mild soap, rinsing thoroughly; apply a good quality skin lotion. In cases of
prolonged, repeated or extensive exposure, rinse affected area or entire body for at least 15 minutes. For severe
conditions, consult a physician.
Ingestion: Call a poison control center immediately. If victim is conscious, have him/her drink several glasses of
water to dilute the solution. Induce vomiting only upon the advice of a physician or poison control authority.
5. FIRE FIGHTING MEASURES
Flammable properties
Flash point: > 145癋 (> 62.8癈) closed cup.
Flammable limit: Not determined.
Autoignition temperature: Not determined.
Flammability classification: Class IIIA Combustible liquid (OSHA).
Flame propagation: None.
Hazardous products of combustion: Carbon monoxide and carbon dioxide.
Extinguishing media: ABC rated portable fire extinguishers should be used. Professional fire fighters
may use water spray, dry chemical or carbon dioxide.
Fire fighting instructions: Sealed chemical suits and self contained breathing apparatus are
necessary for fighting fires involving substantial volumes of this product.
ANATECH LTD. Page 3 of 7
BRAZILLIANT! Date prepared: March 2005
Date Reviewed: May 2008
6. ACCIDENTAL RELEASE MEASURES
Wear protective gloves, impermeable aprons and splash-proof goggles. Use a damp sponge or mop to remove
spilled liquid. Wash contaminated area with water and decolorize with household bleach. Discard absorbents and
other contaminated solids in a trash receptacle. Liquid waste may be discarded down the drain with approval by
wastewater authorities, or may be removed by a licensed waste hauler.
Comply with all applicable governmental regulations on spill reporting and on the handling and disposal of
hazardous waste.
7. HANDLING AND STORAGE
Handling: Wear a plastic or rubber apron, protective gloves and splash-proof goggles. Avoid contact with skin
and eyes. Do not continue to wear contaminated clothing after a spill.
Storage: Store at room temperature.
8. EXPOSURE CONTROLS AND PERSONAL PROTECTION
Engineering controls: Good general room ventilation is essential.
Personal protective equipment
Respiratory protection: None needed.
Skin protection: Anatech Ltd. recommends nitrile gloves. Do not use latex surgical gloves for protection
against any hazardous liquid. An eyewash station and safety shower must be nearby, preferably in the
same room, no more than 10 seconds away.
Eye protection: Use splash-proof goggles. Do not use safety glasses. An eyewash station and safety
shower must be nearby, preferably in the same room, no more than 10 seconds away.
9. PHYSICAL AND CHEMICAL PROPERTIES
Appearance: Red liquid.
Odor: Mild acetic acid odor.
Physical state: Liquid.
pH: 2.4 - 2.7.
Vapor pressure: Not determined.
Vapor density: Not determined.
Boiling point: Not determined.
Freezing point: Not determined.
Solubility in water: Complete.
Specific gravity: Not determined.
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BRAZILLIANT! Date prepared: March 2005
Date Reviewed: May 2008
10. STABILITY AND REACTIVITY
Chemical stability: Stable.
Conditions to avoid: None.
Incompatibility with other materials: Strong oxidants, acids and bases.
Hazardous decomposition products: None.
Hazardous polymerization: None.
11. TOXICOLOGICAL INFORMATION
The following data are from studies using 100% ethanol.
Acute eye effects: 500 mg administered into rabbit eyes for 24 hours produced moderate irritation.
Acute skin effects: Draize test on rabbit skin of 20 mg for 24 hours produced moderate irritation.
Acute oral effects: OSHA considers chemicals to be toxic if their LD50 is at or below 500 mg/kg. LD50 is the
dose killing 50% of the test animals in a given time (usually 4 hours). LD50 was 7,060 mg/kg in rats, 3,450 mg/kg
in mice and 6,300 mg/kg in rabbits.
Acute inhalation effects: OSHA considers chemicals to be toxic if their LC50 is at or below 20 mg/kg. LC50 is
the airborne concentration killing 50% of the test animals. LC50 was 20,000 ppm/10 hours in rats. In man, ethanol
produces severe toxic effects at 8,000 ppm and symptoms of illness at 2,000 ppm.
Chronic effects/carcinogenicity: Chronic ethanol ingestion is associated with liver cancer.
Teratology: None known.
Reproductive effects: Ethanol has been linked to birth defects in humans.
Mutagenicity: None known.
12. ECOLOGICAL INFORMATION
Ecotoxicity: The following data are from studies using 100% ethanol.
Toxicity threshold (cell multiplication inhibition test):
Bacteria
Pseudomonas putida: 6,500 mg/l
Algae
Microcystis aeruginosa: 1,450 mg/l
Scenedesmus quadricauda: 5,000 mg/l
Protozoa
Entosiphon sulcatum: 65 mg/l
Uronema parduczi: 6,120 mg/l
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BRAZILLIANT! Date prepared: March 2005
Date Reviewed: May 2008
12. ECOLOGICAL INFORMATION (continued)
Fish
Fingerling trout: 24 hr LC50: 11,200 mg/l, flow through system
Cheek chub: LD0: 24 hr in Detroit river water: 7,000 mg/l
LD100: 24 hr in Detroit river water: 9,000 mg/l
LC50 > 7,000 mg/l
Guppy: 7 day LC50 = 11,050 mg/l
Fathead minnow: static bioassay in Lake Superior water at 18癈 - 22癈 (1, 24, 48, 72,
96 hours) >18,000, 18,000, 13,480, 13,480, 13,480 mg/l respectively.
Biological Oxygen Demand (BOD), 5 day: = 37-74% ThOD
Chemical Oxygen Demand (COD) = 90-97% ThOD
Environmental fate: Ethanol is readily biodegradable.
13. DISPOSAL CONSIDERATIONS
Brazilliant! is not hazardous under EPA regulations. Drain disposal is recommended with the permission of local
wastewater treatment authorities.
Canadian disposal regulations generally parallel those in the United States.
Follow federal, state (provincial) and local regulations. Proper waste disposal is the generator's responsibility.
14. TRANSPORTATION INFORMATION
DOT (ground and air) and IATA: Not regulated.
15. REGULATORY INFORMATION
OSHA (USA): Under the Hazard Communication Standard and the Laboratory Standard, this product is a
hazardous material: it is an irritant.
The OSHA Standards cited above mandate that exposed workers receive proper training in the properties of this
product, work practices involved with its handling and disposal, and interpretation of its MSDS.
FDA (USA): Brazilliant! is for in vitro diagnostic use as a stain in histology.
EPA (USA): Brazilliant! is a reportable substance under SARA Title III.
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BRAZILLIANT! Date prepared: March 2005
Date Reviewed: May 2008
16. OTHER INFORMATION
Label warnings: Avoid contact with skin and eyes. use with adequate ventilation.
NFPA (National Fire Protection Association) Rating:
General note: This rating is applicable only to safeguard the lives of individuals who may be
concerned with fires occurring in an industrial plant or storage location. The ratings provide information
to emergency personnel on whether to evacuate the area or how to perform control procedures. It is not
descriptive of hazards under normal conditions of occupational use, and is even less applicable to
anticipated laboratory-scale use.
Health 0: Materials that, under emergency conditions, would offer no hazard beyond that of ordinary combustible
materials.
Flammability 1: Materials that must be preheated before ignition can occur.
Instability 0: Materials that are normally stable even under fire conditions.
This Material Safety Data Sheet has been prepared in accordance with the requirements of the OSHA Hazard
Communication Standard. It conforms to the provisions of the American National Standards Institute (ANSI) Standard
Z400.1 (Standard for the Preparation of Material Safety Data Sheets). Information contained herein was obtained from
sources which Anatech Ltd. believes are reliable. It is the user's responsibility to determine suitability of the product for
his/her own use, and to assure proper use and disposal of it to protect the safety and health of employees and the
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BRAZILLIANT! Date prepared: March 2005
Date Reviewed: May 2008
protection of the environment.
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