130 Research Lane, Suite 2 Guelph Ontario N1G 5G3 Canada Tel: (519) 822-2265 Fax: (519) 822-3151
KB-1TM Dechlorinator Material Safety Data Sheet
Section 1: Material Identification
Trade Name: KB-1TM Dechlorinator
Chemical Family: bacterial mixture
Chemical name: No IUC name for mixture is known to exist
Manufacturer/Supplier: SiREM
130 Research Lane, Suite 2,
Guelph, Ontario,
Canada N1G 5G3
For Information call: 519-822-2265 / 1-866-251-1747
Emergency Number: 519-822-2265
Description: Microbial inoculum (non-pathogenic, non-hazardous)
KB-1TM Dechlorinator
Trade Name:
Product Use: Bioremediation of contaminated groundwater.
Date Prepared: 2 February 2005
Section 2: Composition, Information on Ingredients
KB-1鈩? Dechlorinator is a microbial culture grown in an aqueous dilute mineral salt solution media
containing no hazardous ingredients.
The microbial composition of KB-1鈩? Dechlorinator (as determined by phylogenetic analysis) is
listed in Table 1. Identification of organisms was obtained by matching 16S rRNA gene sequence
of organisms in KB-1鈩? Dechlorinator to other known organisms. The characteristics of related
organisms can be used to identify potential or likely characteristics of organisms in KB-1鈩?
Dechlorinator.
Table 1. Genus鈥? identified in KB-1鈩? Dechlorinator Microbial Inoculum
Genus
Dehalococcoides sp.
Geobacter sp.
Methanomethylovorans sp.
Section 3: Hazards Identification:
A review of the available data does not indicate any known health effects related to normal use of
this product.
Section 4: First Aid Measures:
Avoid direct contact with skin and eyes. In any case of any exposure which elicits a response, a
physician should be consulted immediately.
130 Research Lane, Suite 2 Guelph Ontario N1G 5G3 Canada Tel: (519) 822-2265 Fax: (519) 822-3151
Eye Contact: Flush eyes with water for at least 15 minutes, occasionally lift upper and lower
eyelids, if undue irritation or redness occurs seek medical attention.
Skin Contact: Remove contaminated clothing and wash skin thoroughly with water and
antibacterial soap. Seek medical attention if irritation develops or open wounds are present.
Ingestion: Do not induce vomiting, drink several cups of water, seek medical attention.
Inhalation: Remove to fresh air. If not breathing give artificial respiration. In case of labored
breathing give oxygen. Call a physician.
Section 5 - Fire Fighting Measures:
Non-flammable
Flash Point: not applicable
Upper flammable limit: not applicable
Lower flammable limit: not applicable
Section 6 鈥? Accidental Release Procedures
Spilled KB-1TM Dechlorinator should be soaked up with sorbant and saturated with a 10%
bleach solution (prepared by making a one in ten dilution of diluted standard bleach
[normally sold at a strength of 5.25% sodium hypochlorite] to disinfect affected surfaces.
Sorbant should be double bagged and disposed of as indicated in section 12. After
removal of sorbant, area should be washed with 10% bleach solution to disinfect. If liquid
from the culture vessel is present on the fittings, non-designated tubing or exterior of the
stainless steel pressure vessel liquid should be wiped off and the area washed with 10%
bleach solution.
Section 7 - Handling and Storage
KB-1TM Dechlorinator is shipped in stainless steel pressure vessels and connected to
injection lines and inert gas is used to pressurize the vessel to displace the contents. KB-
1TM Dechlorinator should be handled with care to avoid any spillage. Vessels are
shipped with 1 pound per square inch (psi) pressure; valves should not be opened until
connections to appropriate lines for subsurface injection are in place.
Storage Requirements: Avoid exposing stainless steel pressure vessels to undue
temperature extremes (i.e., temperatures less than 0掳C or greater than 30掳C may result
in harm to the microbial cultures and damage to the vessels). All valves should be in the
closed position when the vessel is not pressurized to prevent the escape of gases and to
maintain anaerobic conditions in the vessel. Avoid exposure of the culture to air as the
presence of oxygen will kill dechlorinating microorganisms.
Section 8 - Exposure Controls/Personal Protection
Personal protective equipment:
Skin: Protective gloves (latex, vinyl or nitrile) should be worn.
Eye Protection: Wear appropriate protective eyeglasses or goggles when opening
pressure vessels valves or when pressurizing vessels to inject contents into the
subsurface.
Respiratory: No respiratory protection is required.
Engineering Controls: Good general room ventilation is expected to be adequate.
130 Research Lane, Suite 2 Guelph Ontario N1G 5G3 Canada Tel: (519) 822-2265 Fax: (519) 822-3151
Section 9: Physical and Chemical Properties:
Physical State: liquid
Odour: skunky odour
Appearance: dark grey, slightly turbid liquid under anaerobic conditions, pink if exposed
to air (oxygen).
Specific gravity: not determined
Vapor pressure: not applicable
Vapor density: not applicable
Evaporation rate: not determined
Boiling point: ~100掳 C
Freezing point/melting point: ~ 0掳C
pH: 6.5-7.5
Solubility: fully soluble in water
Section 10 鈥? Stability and Reactivity Data
Stable and non-reactive.
Maintain under anaerobic conditions to preserve product integrity.
Materials to avoid: none known
Section 11 - Toxicological Information
Potential for Pathogenicity:
KB-1鈩? Dechlorinator has tested negative (i.e., the organisms are not present) for a variety of
pathogenic organisms listed in Table 2. While there is no evidence that virulent pathogenic
organisms are present in KB-1TM Dechlorinator, there is potential that certain organisms in KB-
1鈩? Dechlorinator may have the potential to act as opportunistic (mild) pathogens, particularly in
individuals with open wounds and/or compromised immune systems. For this reason standard
hygienic procedures such as hand washing after use should be observed.
Table 2, Results of Human Pathogen Screening of KB-1TM Dechlorinator
Disease(s) Caused
Organism Test result
Salmonella sp. Typhoid fever, gastroenteritis Not Detected
Listerioses Not Detected
Listeria monocytogenes
Cholera, gastroenteritis Not Detected
Vibrio sp.,
Bacterial diarrhea Not Detected
Campylobacter sp.,
Food poisoning, Botulism, tetanus, gas gangrene Not Detected
Clostridia sp.,
Anthrax Not Detected
Bacillus anthracis
Wound infection Not Detected
Pseudomonas aeruginosa
Bubonic Plague, intestinal infection Not Detected
Yersinia sp.,
Yeast and Mold Candidiasis, Yeast infection etc. Not Detected
Fecal coliforms Indicator organisms for many human pathogens diarrhea, Not Detected
urinary tract infections
Various opportunistic infections Not Detected
Enterococci
130 Research Lane, Suite 2 Guelph Ontario N1G 5G3 Canada Tel: (519) 822-2265 Fax: (519) 822-3151
Section 12. Disposal Considerations
Material must be disinfected or sterilized prior to disposal. Consult local regulations prior to
disposal.
Section 13 鈥? Transport Information
Non-hazardous, non-pathogenic microbial inoculum 鈥? Biosafety Risk Group 1.
Chemicals, Not Otherwise Indexed (NOI), Non-hazardous
Not subject to TDG or DOT guidelines.
Disclaimer:
The information provided on the MSDS sheet is based on current data and represents
our opinion based on the current standard of practice as to the proper use and handling
of this product under normal, reasonably foreseeable conditions.
Last revised: 3 February 2005
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