EMS CATALOG NO:       19020

                                                EMS PRODUCT:        Nile Blue A

                                                     DATE:          09/22/2008

                                                  PAGE NUMBER:         One of 4





                          MATERIAL SAFETY DATA SHEET



The information contained herein is based on data considered accurate. However, no

warranty is expressed or implied regarding the accuracy of these data or the results to

obtained from the use thereof.



Electron Microscopy Sciences assumes no responsibility for personal injury or property

damage to vendees, users or third parties caused by the material. Such vendees or users

assume all risks associated with the use of the material.





ELECTRON MICROSCOPY SCIENCES

1560 INDUSTRY ROAD

P.O. BOX 550

HATIFIELD, PA 19440           24 HOUR EMERGENCY PHONE NUMBER

(215) 412-8400                            CHEMTREC: (800) 424-9300

_________________________________________________________________



                FOR PRODUCT AND SALES INFORMATION

_________________________________________________________________



       CONTACT ELECTRON MICROSCOPY SCIENCES OFFICE ABOVE.



_________________________________________________________________



                       PRODUCT IDENTIFICATION

_________________________________________________________________



PRODUCT NAME:                         Nile Blue A, Certified



CAS NUMBER:                           3625-57-8



MOLECULAR FORMULA:                    C40H40N6O6S



SYNONYMS:         5H-Benzo (A) Phenazoxazine, 9-(Diethylamino)-5-Imino-,

                  Sulfate (2:1); Nile Blue A Sulfate; Nile Blue Sulfate

________________________________________________________________



                              TOXICITY HAZARDS

________________________________________________________________

RTECS NUMBER:                         DJ1931000



      Benzo(A) Phenoxazin-7-Ium, 5-Amino-9-(Diethylamino)-,

      Sulfate (2:1)





REVIEWS, STANDARDS, AND REGULATIONS:



NOES 1983: HZD X4351000; TNF 1; NIS 6; NOS 1; TNE 39; TFE 20



      Only selected Registry of Toxic Effects of Chemical Substances

      (RTECS) data is presented here. See actual entry in RTECS for

      complete information.

________________________________________________________________



                         HEALTH HAZARD DATA

________________________________________________________________



ACUTE EFFECTS:



      May be harmful by inhalation, ingestion, or skin absorption.

      May cause eye irritation. May cause skin irritation.



      To the best of our knowledge, the chemical, physical, and

      toxicological properties have not been thoroughly

      investigated.



FIRST AID:



      In case of contact, immediately flush eyes or skin with copious

      amounts of water for at least 15 minutes while removing

      contaminated clothing and shoes.



      If inhaled, remove to fresh air. If not breathing give

      artificial respiration. If breathing is difficult, give oxygen.



      If swallowed, wash out mouth with water provided person is

      conscious.



      Call a physician. Wash contaminated clothing before reuse.

________________________________________________________________



                            PHYSICAL DATA

________________________________________________________________









                                                                        2

                                   > 300oC

MELTING POINT:



APPEARANCE AND ODOR:          Dark-green powder

________________________________________________________________



                  FIRE AND EXPLOSION HAZARD DATA

________________________________________________________________



EXTINGUISHING MEDIA:



      Water spray. Carbon dioxide, dry chemical powder, alcohol or

      appropriate foam.





SPECIAL FIREFIGHTING PROCEDURES:



      Wear self-contained breathing apparatus and protective clothing

      to prevent contact with skin and eyes.



UNUSUAL FIRE AND EXPLOSIONS HAZARDS:



      Emits toxic fumes under fire conditions.

________________________________________________________________



                           REACTIVITY DATA

________________________________________________________________



INCOMPATIBILITIES:                 Strong oxidizing agents



HAZARDOUS DECOMPOSITION OR DECOMPOSITION PRODUCTS:



      Thermal decomposition may produce carbon monoxide, carbon

      dioxide, nitrogen oxides, and sulfur oxides.

________________________________________________________________



                      SPILL OR LEAK PROCEDURES

________________________________________________________________



STEPS TO BE TAKEN IF MATERIAL IS RELEASED OR SPILLED:



      Wear self-contained breathing apparatus, rubber boots and heavy

      rubber gloves. Wear disposable coveralls and discard them after

      use. Sweep up, place in a bag and hold for waste disposal.

      Avoid raising dust. Ventilate area and wash spill site after

      material pickup is complete.









                                                                        3

WASTE DISPOSAL METHOD:



      Dissolve or mix the material with a combustible solvent and burn

      in a chemical incinerator equipped with an afterburner and

      scrubber.



      Observe all Federal, State and local environmental regulations.

________________________________________________________________



        PRECAUTIONS TO BE TAKEN IN HANDLING AND STORAGE

________________________________________________________________



      Wear appropriate NIOSH/MSHA-approved respirator, chemical-

      resistant gloves, safety goggles, other protective clothing.

      Use only in a chemical fume hood.

      Safety shower and eye bath.

      Do not breathe dust.

      Avoid contact with eyes, skin and clothing.

      Wash thoroughly after handling.

      Strong dye. Keep tightly closed. Store in a cool dry place.









                                                                         4