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MSDS #: 58
MATERIAL SAFETY DATA SHEET
Trade Name: Cap A (89)
Section I - Chemical Products & Company Identification
Proligo LLC Proligo Biochemie GmbH Creation Date: 2/99
6200 Lookout Rd Georg-Heyken-Str. 14 Revision Date: 3/04
Boulder, Colorado 80301 USA Hamburg, Germany D21147 Revision Number: 1
Product #: L040250, L040400, L043000, L840020, L840045
For emergency source information (USA) contact: Chemtrec 1-800-424-9300 24 Hours
For emergency source information (International) contact: Chemtrec 001-703-527-3887 (USA) 24 Hours
Trade Names/Synonyms: Cap A/DNA Synthesis Reagent
Chemical Family: Nucleotide Synthesis Reagent Mixture
Section 2 - Composition/Information On Ingredients - % by Weight
Chemicals: Composition (w/w) %: CAS Number: Classification: Odor Threshold:
F; R: 11-19 Xi; R:
50-100% 109-99-9 20-50 ppm
Tetrahydrofuran
36/37
F; R: 10 C; R:34
10-25% 108-24-7 0.14 ppm
Acetic Anhydride
Section 3 - Hazards Identification
NFPA (National Fire Protection Association) Ratings (Scale 0-4): Health: 2 Flammable: 3 Reactivity: 1
Emergency Overview: Irritating to the eyes and respiratory system. The toxicological properties of this material
have not been thoroughly investigated as a mixture. In case of contact with eyes, rinse immediately with plenty of
water and seek medical advice. Use prudent laboratory practices for handling chemical substances. Keep substance
away from sources of ignition-No Smoking. Highly flammable (EU). May form explosive peroxides. Take
precautionary measures against static discharges.
Potential Health Effects:
Short Term Exposure: Irritant
Skin Contact:
Long Term Exposure: May be harmful, causes defatting and dermatitis.
Short Term Exposure: Irritant
Eye Contact:
Long Term Exposure: May be harmful
Short Term Exposure: Irritant, can cause narcotic effects, coughing, chest pains, difficulty
Ingestion:
breathing, nausea, dizziness, headache, and unconsciousness.
Long Term Exposure: Maybe harmful to the liver, kidneys, and central nervous system.
Short Term Exposure: Irritant, can cause narcotic effects, coughing, chest pains, difficulty
Inhalation:
breathing, nausea, dizziness, headache, and unconsciousness.
Long Term Exposure: Maybe harmful to the respiratory system.
Short Term Exposure: No information available
Injection:
Long Term Exposure: No information available
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MSDS #: 58
MATERIAL SAFETY DATA SHEET
Trade Name: Cap A (89)
CARCINOGEN/REPRODUCTIVE TOXIN STATUS:
Tetrahydrofuran Acetic Anhydride
OSHA: Not Listed Not Listed
NTP: Not Listed Not Listed
IARC: Not Listed Not Listed
According to 91/155 EC:
Hazard designation:
Xi Irritant
F Highly flammable
Information pertaining to particular dangers for man and environment
R 11 Highly Flammable
R 19 May form explosive peroxides
R 37/38 Irritating to respiratory system and skin
R 41 Risk of serious damage to eyes
*Classification system
The classification is in line with current EC lists. It is expanded, however, by information from technical literature
and by information furnished by supplier companies
Section 4: First Aid Measures
Remove contaminated clothing. Flush affected area with water and then wash with soap or
Skin Contact:
mild detergent and water. Observe for signs of irritation. If irritation is present, get medical
attention.
Wash eyes with large amounts of water or normal saline for at least 15 minutes. Observe for
Eye Contact:
signs of irritation. If irritation is present, get medical attention.
Get medical attention.
Ingestion:
Remove from exposure area to fresh air immediately. Get medical attention immediately.
Inhalation:
If accidentally injected, get medical attention.
Injection:
There is no specific antidote. Treat symptomatically and supportively.
Note to Physician:
Section 5: Fire Fighting Measures
FIRE AND EXPLOSION HAZARD: Flammable and irritating liquid. Can form heat-sensitive peroxides, which could explode during
concentration or evaporation. Explosion Limits: LEL 2% and UEL 11.8% for Tethrahydrofuran. Explosion Limits: LEL 2.7%
and UEL 10.3% for Acetic Anhydride.
Carbon dioxide, dry chemical powder or appropriate foam. Water may be effective for cooling, but may not
EXTINGUISHING MEDIA:
effect extinguishment.
SPECIFIC HAZARDS: Vapor may travel considerable distance to source of ignition and flash back. Container explosion may occur under
fire conditions. Forms explosive mixtures with air. Emits toxic fumes under fire conditions.
Wear self-contained breathing apparatus and protective clothing to prevent
PROTECTION OF FIREFIGHTERS:
contact with skin and eyes. Use water spray to cool fire-exposed containers.
Section 6: Accidental Release Measures
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MSDS #: 58
MATERIAL SAFETY DATA SHEET
Trade Name: Cap A (89)
OCCUPATIONAL SPILL: Wear appropriate protective clothing and chemically compatible gloves. Remove all
sources of ignition. Place spillage in appropriate container for waste disposal. Wash contaminated clothing before
reuse. (See Section 8)
US CERCLA REPORTABLE QUANTITY (RQ): 1000 lbs. (RCRA code U213) for Tetrahydrofuran and 5000 lbs for
Acetic Anhydride.
Section 7 - Handling and Storage
Observe all federal, state and local regulations. Do not breathe fumes. Avoid contact with eyes, skin and clothing.
Wash thoroughly after handling. Keep tightly closed. Store in cool dry place. Use prudent laboratory practices for
handling and storage of chemical substances. Avoid sources of ignition.
Section 8 - Exposure Controls/Personal Protection
Use in laboratory hood or other ventilated device. Wear appropriate approved respirator where no engineering controls
Exposure
exist. Avoid contact with sources of ignition. Use non-sparking tools.
Controls:
Employees must wear splash-proof safety goggles to prevent eye contact with this substance.
Eye Protection:
Employee must wear appropriate protective clothing (laboratory coat with long sleeves) and equipment to prevent skin
Clothing:
contact with this material.
Employee must wear appropriate protective gloves (Nitrile Gloves, 0.33 mm Thickness) to prevent contact with this
Gloves:
material.
Permissible Exposure Limits (USA):
Tetrahydrofuran Acetic Anhydride
200 5
ACGIH TWA (ppm)
250 N/A
ACGIH STEL/C (ppm)
2000 [10% LEL] 200
IDHL (ppm)
200 5
OSHA (ppm)
200 5
NIOSH (ppm)
Permissible Exposure Limits (Europe):
Tetrahydrofuran Acetic Anhydride
5 (ceiling value)
Germany (ppm) 200
100 mg/ m3 (work place air) 3
USSR-UNEP/IRPTC (MAC) 0.03 mg/ m (average residential air)
200 N/A
U. K. TWA (ppm)
N/A 5
U. K. STEL (ppm)
According to 91/155 EC:
Components with limit values that require monitoring at the workplace:
Tetrahydrofuran (CAS # 109-99-9) 50-100%
OEL: Short-term value: 735 mg/m3, 250 ml/ m3
Long-term value: 590 mg/m3 , 200 ml/ m3
Acetic Anhydride (CAS # 108-24-7) 10-25%
OEL: Short-term value: 20 mg/m3 , 5 ml/ m3
Long-term value: 20 mg/m3 , 5 ml/ m3
*Additional information about design of technical systems:
No further data; see item 7
Section 9 - Physical and Chemical Properties
DESCRIPTION: Liquid, Colorless, with pungent odor
SOLUBILITY: Miscible with organic solvents and water ( the mixture)
FLASHPOINT: -14.4癈(6.08癋)( for Tetrahydrofuran) and 48.9癈(120癋)( for Acetic Anhydride)
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MATERIAL SAFETY DATA SHEET
Trade Name: Cap A (89)
BOILING POINT: 65癈 (149癋)AT 1000 mBar ( for Tetrahydrofuran)
and 138.9癈(282癋) ( for Acetic Anhydride)
MELTING POINT: -73癈(-99.4癋) (for Acetic Anhydride)
FREEZING POINT: < -20.0癈(<-4癋) ( for Tetrahydrofuran)and ?2.8癈(-99癋) ( for Acetic Anhydride)
VAPOR PRESSURE: 190 mBar at 15.0癈(59癋) ( for Tetrahydrofuran) and 4 mm (for Acetic Anhydride)
VAPOR DENSITY: 2.5 (Tetrahydrofuran, air = 1)
pH: pH 3 at 100 g/L in water(20癈, 68癋)
May form explosive peroxides
Danger of Explosion:
Self-inflammability: Product is not selfigniting
Solvent Content: 0% Water
Section 10 - Stability and Reactivity
STABILITY: Stable at room temperature.
REACTIVITY: Incompatible with bases, slow degradation in water. Acetic anhydride reacts
with water to acetic acid.
CONDITIONS TO AVOID: Incompatible with bases, strong oxidizers, lithium-aluminum alloys, chromic
acid, strong caustics, and amines. Exposure to a heat source. Peroxides may
accumulate upon prolonged storage in presence of air. Acetic anhydride is
corrosive to iron, steel, and other metals.
INCOMPATIBILITIES: Forms explosive mixture with air. Unless inhibited, can form unstable and
explosive peroxides. Incompatible with strong acids, strong oxidizers. Attacks
some plastics. May accumulate static electric charges that can result in ignition
of its vapors.
DECOMPOSITION PRODUCTS: May include toxic oxides of carbon and nitrogen.
POLYMERIZATION: Not available
Section 11 - Toxicology Information
The toxicological properties of this material have not been investigated as a mixture.
TOXICITY:
Tetrahydrofuran: LD50 Oral (Rat)=2816 mg/ kg
LC50 Inhalation (Rat)=21000 ppm/ 3 hours.
Human lethal dose 50-500 mg/kg.
Acetic anhydride: LD50 Oral (Rat)=1780 mg/ kg
LC50 Inhalation (Rat)=1000 ppm/ 4 hours.
CARCINOGEN STATUS: No information available
LOCAL EFFECTS: See Section 3 for health effects.
TARGET EFFECTS: Eyes, liver (fatty liver degeneration) respiratory system, and central nervous system
(CNS)
AT INCREASED RISK Effects on fertility may cause post-implantation mortality.
FROM EXPOSURE:
Chemical Name: RTECS Number:
LU5950000
Tetrahydrofuran
AK1925000
Acetic Anhydride
According to 91/155 EC:
Contains larger amounts of solvent, which may cause narcotic effects, headache, unconsciousness, dizziness, lack of
concentration, weakness, difficulty breathing, coughing or nausea.
*Additional toxicological information:
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MSDS #: 58
MATERIAL SAFETY DATA SHEET
Trade Name: Cap A (89)
The product shows the following dangers according to the calculation method of the General EC Classification
Guidelines for Preparations as issued in the latest version;
Irritant
Section 12 - Ecological Information
ENVIRONMENTAL IMPACT RATING (0-4): No data available.
ACUTE AQUATIC TOXICITY: Tetrahydrofuran:
EPA: 8,100 礸/L (no set criteria, permissible ambient goal)
USSR-UNEP/IRPTC (MAC): 0.5 mg/L
Acetic Anhydride: no criteria set
DEGRADABILITY: No data available.
LOG BIOCONCENTRATION FACTOR (BCF): No data available.
Section 13 - Disposal Information
Observe all Federal, State, and Local Regulations.
According to 91/155 EC:
Product:
Recommendation: Must not be disposed of together with household garbage. Do not allow product to reach sewage
system.
Uncleaned packaging:
Recommendation: disposal must be made according to official regulations.
Recommended cleaning agent: Water, if necessary with cleaning agent
Before cleaning use up or empty container completely.
Section 14 - Transportation Information
Ship as regulated material. Transportation restrictions apply. The acute toxicity profile does warrant shipment as a
hazardous material (DOT) or a dangerous good (IATA).
UN2924 Hazard Class 3 Subsidiary Risk 8
Label Requirements: Flammable liquid, corrosive, n.o.s. (Tetrahydrofuran and Acetic Anhydride)
According to 91/155 EC:
Land transport ADR/RID(cross-border)
?ADR/RID-GGVS/E Class: 3 Inflammable liquids
?Number/Letter: 26b
?Kemler Number: 338
?UN-Number: 2924
?Label 3+8
?Designation of goods: Flammable liquid, corrosive, n.o.s.,
tetrahydrofuran, acetic anhydride
Maritime transport IMDG:
?IMDG Class: 3.2
?Page: 3231
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MATERIAL SAFETY DATA SHEET
Trade Name: Cap A (89)
?UN Number: 2924
?Packaging group: II
?EMS Number: 3-02
?MFAG: 760
?Correct technical name: Flammable liquid, corrosive, n.o.s.,
tetrahydrofuran, acetic anhydride
Air transport ICAO-TI and IATA-DGR:
?ICAO/IATA Class: 3
?UN/ID Number: 2924
?Packaging group: II
?Correct technical name: Flammable liquid, corrosive, n.o.s.,
tetrahydrofuran, acetic anhydride
Section 15 - Regulatory Information
Tetrahydrofuran Acetic Anhydride
Europe:
203-726-8 203-564-8
EINECS/ELINCS:
EC INDEX NO. 603-025-00-0 607-008-00-9
Canada:
NDSL/DSL: DSL DSL
United States:
TSCA STATUS: Yes, 4/12b, Notice of Export Yes
CERCLA SECTION 103 (40 CFR 302.4):
1000 lbs. 5000 lbs.
SARA SECTION 302 (40 CFR 355.30): No No
SARA SECTION 304 (40 CFR 355.40): No No
SARA SECTION 313 (40 CFR 372.65): No No
No No
OSHA PROCESS SAFETY (29 CFR 1910.119):
CALIFORNIA PROPOSITION 65 No No
Yes
SARA ACUTE HAZARD (40 CFR 370.21) No
Yes
CHRONIC HAZARD: No
Yes No
FIRE HAZARD:
REACTIVITY HAZARD: Yes No
SUDDEN RELEASE HAZARD: No No
Classification:
F; R;11/19 Xi; R: 36/37/38
Symbols: F (Flammable) and Xi (Irritant)
Risk Phrase: Mixture
R: 11
Highly Flammable
R: 19
May form explosive peroxides
R: 37/38
Irritating to respiratory system and skin
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MATERIAL SAFETY DATA SHEET
Trade Name: Cap A (89)
R: 41
Risk of serious damage to eyes
Safety Phrases: Mixture
S:16
Keep away from sources of ignition- No Smoking.
S:26
In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
S:33
Take precautionary measures against static discharges.
For Laboratory R&D Use Only.
According to 91/155 EC:
National Regulations
Classification according to VbF: B
Technical instructions (air):
Class Share in %: II 50-100
Section 16 - Other
THE AFOREMENTIONED INFORMATION IS BELIEVED TO BE CORRECT, BUT DOES NOT
PURPORT TO BE ALL INCLUSIVE AND SHALL BE USED ONLY AS A GUIDE. PROLIGO LLC
SHALL NOT BE HELD LIABLE FOR ANY DAMAGE RESULTING FROM HANDLING OR FROM
CONTACT WITH THE ABOVE PRODUCT.
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