9. Timer
10. Xylene
11. Ethanol or reagent alcohol
iVIEWâ„? DAB Detection Kit 12. Deionized or distilled water
Biocare Medical’s Decloaking Chamber (ES® and NexES IHC® automated slide
13.
Catalog number 760-091 stainers)
ES®, NexES IHC®, BenchMarkTM and BenchMarkTM XT automated slide stainers
14.
15. Ventana Endogenous Biotin Blocking Kit
INDICATIONS AND USE
16. Detection kit specific software (ES automated slide stainer only)
Intended Use
17. Ventana APK Wash Solution (ES and NexES IHC automated slide stainers)
This detection kit is intended for in vitro diagnostic use.
Ventana Liquid CoverslipTM solution (ES and NexES IHC automated slide stainers)
18.
Ventana Medical Systems' (Ventana®) iVIEWTM DAB Detection Kit is an indirect biotin
Ventana EZ PrepTM solution (BenchMark and BenchMark XT automated slide
19.
streptavidin system for detecting mouse IgG, mouse IgM and rabbit polyclonal primary
stainers)
antibodies. The kit is intended for laboratory use to identify targets in sections of formalin
20. Ventana Reaction Buffer (BenchMark and BenchMark XT automated slide stainers)
fixed, paraffin embedded and frozen tissue on Ventana Medical Systems (Ventana®)
21. Ventana LCS (BenchMark and BenchMark XT automated slide stainers)
automated slide stainers by light microscopy.
22. Ventana Cell Conditioning 1 (CCI) (BenchMark and BenchMark XT automated slide
The clinical interpretation of any staining, or the absence of staining, must be
stainers)
complemented by morphological studies and evaluation of proper controls. Evaluation must
23. Ventana Cell Conditioning 2 (CC2) (BenchMark and BenchMark XT automated slide
be made by a qualified pathologist within the context of the patient’s clinical history and
stainers)
other diagnostic tests. Caution: U.S. Federal law restricts this device to sale by or on the
24. Ventana Protease I
order of a physician.
25. Ventana Protease II
26. Ventana Protease III
Summary and Explanation
27. Ventana Hematoxylin Counterstain
The iVIEW DAB Detection Kit utilizes biotinylated secondary antibodies to locate the bound
28. Ventana Bluing Reagent
primary antibody, followed by the binding of Streptavidin-HRP (horseradish peroxidase)
29. Mounting medium
conjugate. The complex is then visualized with hydrogen peroxide substrate and 3, 3�-
30. Cover glass
diaminobenzidine tetrahydrochloride (DAB) chromogen, which produces a dark brown
31. Light microscope (20-80X)
precipitate which is readily detected by light microscopy.
Storage and Handling
Principles and Procedures
The iVIEW DAB Detection Kit detects specific mouse IgG, IgM and rabbit IgG antibodies Store at 2-8° C. Do not freeze. The user must validate any storage conditions other than
bound to an antigen in paraffin embedded or frozen tissue sections. The specific antibody is those specified in the package insert. This detection kit can be used immediately after
located by a biotin conjugated secondary antibody. This step is followed by the addition of a removal from the refrigerator.
streptavidin enzyme conjugate which binds to the biotin present on the secondary antibody. To ensure proper reagent delivery and stability of the antibody after every run, the cap must
The complex is then visualized utilizing a precipitating enzyme product. be replaced and the dispenser must be immediately placed in the refrigerator in an upright
Each step is incubated for a precise time and temperature. At the end of each incubation position.
step, the Ventana automated slide stainer washes the sections to remove unbound material Every antibody dispenser is expiration dated. When properly stored, the reagent is stable to
and applies a liquid coverslip which minimizes the evaporation of the aqueous reagents the date indicated on the label. Do not use reagent beyond the expiration date for the
from the slide.1 Results are interpreted using a light microscope and aid in the differential prescribed storage method.
There are no definitive signs to indicate instability of this product; therefore, positive and
diagnosis of pathophysiological processes, which may or may not be associated with a
negative controls should be run simultaneously with unknown specimens. Your local
particular antigen.
Ventana office should be contacted immediately if there is an indication of reagent
For more detailed information on instrument operation, refer to the appropriate Ventana
instability.
Automated Slide Stainer Operator's Manual.
Specimen Collection and Preparation for Analysis
MATERIALS AND METHODS
Routinely processed, formalin fixed, paraffin embedded tissues and frozen tissues are
Reagents Provided
suitable for use when used with iVIEW DAB Detection Kit and Ventana automated slide
iVIEW DAB Detection Kit (250 tests) contains:
stainer (see Materials and Reagents Needed, But Not Provided section). The
1 - dispenser (25 ml) Inhibitor, 3% hydrogen peroxide solution
recommended tissue fixative is 10% neutral buffered formalin.2 Variable results may occur
1 - dispenser (25 ml) Biotinylated Ig Secondary Antibody; affinity purified goat-anti-mouse
IgG and IgM, (<200 µg/ml) and goat-anti-rabbit IgG (<200 µg/ml) in phosphate buffer with a as a result of prolonged fixation or special processes such as decalcification of bone
preservative marrow preparations.
1 - dispenser (25 ml) SA-HRP; a conjugated streptavidin horseradish peroxidase (<300 Each section should be cut the appropriate thickness and placed on a positively charged
µg/ml) solution with protein stabilizer and a preservative glass slide. Slides containing the tissue section may be baked for at least 2 hours (but not
1 - dispenser (25 ml) H2O2; 0.04% to 0.08% hydrogen peroxide in a stabilizing solution longer than 24 hours) in a 70° C ± 5° C oven.
1 - dispenser (25 ml) DAB Substrate; diaminobenzidine (2 g/L) in a stabilizer solution with
preservative Frozen sections should be cut the appropriate thickness, picked up on a negatively charged
1 � dispenser (25 ml) Copper; copper sulfate (5 g/L) in a buffered solution with preservative glass slide and immediately placed in cold acetone (4° to 8 ° C) for ten minutes. The
sections are then air dried for a minimum of thirty minutes and preferably overnight. Apply
appropriate barcode label to dry slides.
Reconstitution, Mixing, Dilution, Titration
The detection kit is optimized for use on a Ventana automated slide stainer. No
reconstitution, mixing, dilution, or titration of kit reagents is required. Manual Deparaffinization Procedure
Further dilution may result in loss of antigen staining. The user must verify any such Required when using the ES or NexES IHC automated slide stainers or if deparaffinization
changes. Differences in tissue processing and technical procedure in the laboratory may is not selected on the BenchMark or the BenchMark XT automated slide stainer:
produce significant variability in results and require regular use of controls. (See Quality 1. For instructions on when to label slides with bar code label, refer to the Instructions
Control Procedures section.) for Use section of the specific automated slide stainer.
2. Immerse the slides sequentially in 3 xylene baths for 5 ± 1 minutes each.
3. Transfer the slides to 100 % ethanol and immerse sequentially in 2 baths for 3 ± 1
Materials and Reagents Needed But Not Provided
The following reagents and materials may be required for staining but are not provided with minutes each.
the detection kit: 4. Transfer the slides to 95% ethanol and immerse them in a bath of this solution for
1. Ventana Negative Control Reagent 3 ± 1 minutes.
2. Ventana Rabbit Negative Control 5. Transfer the slides to 80% ethanol and immerse them in this solution for 3 ± 1
3. Microscope slides, positively charged minutes.
4. Positive and negative tissue controls 6. Transfer the slides to a bath of deionized or distilled water and dip a minimum of 10
5. Drying oven capable of maintaining a temperature of 70° C ± 5° C times.
6. Bar code labels (appropriate bar code labels for negative control and the primary 7. Transfer slides to APK Wash (1X) solution or buffer solution as appropriate. For
antibody being tested) APK Wash solution, the slides should remain until you are ready to perform the
7. 10% neutral buffered formalin staining run. For buffer solution, the slides should remain until you are ready to
8. Staining jars or baths perform the antigen unmasking procedure. Do not allow the slides to dry.
2003-11-30 Page 1 of 4 14910EN Rev A
Slides stained on the BenchMark or BenchMark XT automated slide stainers can be Quality Control Procedures
deparaffinized on the instrument. If this option is selected, barcode slides and place them Positive Tissue Control
on the instrument. If the option is not selected follow the Manual Deparaffinization A positive tissue control must be run with every staining procedure performed. The positive
Procedure above. staining tissue components are used to confirm that the antibody was applied and the
instrument functioned properly. This tissue may contain both positive and negative staining
cells or tissue components and serve as both the positive and negative control tissue.
WARNINGS AND PRECAUTIONS
1. Take reasonable precautions when handling reagents. Use disposable gloves when Control tissues should be fresh autopsy, biopsy or surgical specimens prepared or fixed as
handling suspected carcinogens or toxic materials (example: xylene or soon as possible in a manner identical to the test sections. Such tissues may monitor all
formaldehyde). steps of the procedure, from tissue preparation through staining. Use of a tissue section
2. Avoid contact of reagents with eyes and mucous membranes. If reagents come in fixed or processed differently from the test specimen will provide control for all reagents and
contact with sensitive areas, wash with copious amounts of water. method steps except fixation and tissue processing.
3. Patient specimens and all materials contacting them should be handled as A tissue with weak positive staining is more suitable for optimal quality control and for
biohazardous materials and disposed of with proper precautions. Never pipette by detecting minor levels of reagent degradation.
mouth. Known positive tissue controls should be utilized only for monitoring the correct
4. Avoid microbial contamination of reagents, as this could produce incorrect results. performance of processed tissues and test reagents, not as an aid in determining a specific
5. Incubation times and temperatures other than those specified may give erroneous diagnosis of patient samples. If the positive tissue controls fail to demonstrate positive
results. The user must validate any such change. staining, results with the test specimens should be considered invalid.
6. The reagents have been optimally diluted, and further dilution may result in loss of
antigen staining. The user must validate any such change. Negative Tissue Control
7. When used according to instructions, this product is not classified as a hazardous The same tissue used for the positive tissue control may be used as the negative tissue
substance. The preservative in the reagent is ProClin 300. Symptoms of control. The variety of cell types present in most tissue sections offers internal negative
overexposure to ProClin 300 include skin and eye irritation, and irritation of mucous control sites, but this should be verified by the user. The components that do not stain
membranes and upper respiratory tract. The concentration of ProClin 300 in this should demonstrate the absence of specific staining, and provide an indication of non
product is 0.05% and does not meet the OSHA criteria for a hazardous substance. specific background staining. If specific staining occurs in the negative tissue control sites,
Systemic allergic reactions are possible in sensitive individuals. results with the patient specimens should be considered invalid.
8. Possible carcinogen. The National Toxicology Program has listed benzidine, a closely
related compound to 3, 3�-diaminobenzidine tetrahydrochloride (DAB), as a known Unexplained Discrepancies
human carcinogen. Concentrated forms of propylene glycol have been associated Unexplained discrepancies in controls should be referred to your local Ventana office
with teratogenic effects in laboratory animals. Use disposable neoprene gloves and immediately. If quality control results do not meet specifications, patient results are invalid.
take reasonable precautions when handling. See the Troubleshooting section of this insert. Identify and correct the problem, then repeat
the patient samples.
INSTRUCTIONS FOR USE
Negative Reagent Control
Step by Step Procedure
A negative reagent control must be run for every specimen to aid in the interpretation of
Ventana iVIEW DAB Detection Kits have been developed for use on a Ventana automated
results. A negative reagent control is used in place of the primary antibody to evaluate
slide stainers in combination with Ventana primary anitbodies and accessories. The
nonspecific staining. The slide should be stained with Negative Control Reagent (mouse) or
parameters for the automated procedures can be displayed, printed and edited according to
Rabbit Negative Control, as appropriate. If an alternative negative reagent control is used,
the procedure in the Operator's Manual for the individual automated slide stainer. Other
dilute to the same concentration as the primary antibody antiserum with Ventana Antibody
operating parameters for the automated slide stainers have been preset at the factory.
Diluent. The diluent alone may be used as an alternative to the previously described
negative reagent controls. The incubation period for the negative reagent control should
The procedures for staining on the Ventana automated slide stainers are as follows. For
equal the primary antibody incubation period.
more detailed instructions and additional protocol options refer to your Operator’s Manual.
When panels of several antibodies are used on serial sections, a negative reagent control
on one slide may serve as a negative or nonspecific binding background control for other
ES and NexES IHC Automated Slide Stainers
antibodies.
Antigen Unmasking Required:
1. Slides are deparaffinized through a series of xylene and gradient alcohols to water
Assay Verification
and appropriate buffer. Perform antigen unmasking procedure and transfer slides to
Prior to initial use of an antibody or staining system in a diagnostic procedure, the specificity
APK Wash (1X).
of the antibody should be verified by testing it on a series of tissues with known
2. Load the primary antibody and appropriate detection kit dispensers and required
immunohistochemistry performance characteristics representing known positive and
accessory reagents onto the reagent tray and place them on the automated slide
negative tissues (refer to the Quality Control Procedures previously outlined in this section
stainer. Check bulk fluids and waste.
of the product insert and to the Quality Control recommendations of the College of
Dry the painted end of the slide and then apply slide bar code label which
3.
American Pathologists Laboratory Accreditation Program, Anatomic Pathology Checklist3,
corresponds to the antibody protocol to be performed.
4. Load the deparaffinized, antigen unmasked labeled slides from the APK Wash (1X) or the NCCLS Approved Guideline4 or both documents). These quality control procedures
Avoid tissue drying. should be repeated for each new antibody lot, or whenever there is a change in assay
Antigen Unmasking Not Required: parameters. Tissues listed in the Summary of Expected Results section are suitable for
1. Apply barcode label to slides. Slides are deparaffinized through a series of xylene assay verification.
and gradient alcohols to water. Place sides in APK Wash (1X).
2. Load the primary antibody and appropriate detection kit dispensers and required Interpretation of Results
accessory reagents onto the reagent tray and place them on the automated slide The Ventana iVIEW DAB Detection Kit causes a brown colored reaction product to
stainer. precipitate at the antigen sites localized by the primary antibody. A qualified pathologist who
3. Load the deparaffinized, antigen unmasked (when appropriate) labeled slides from is experienced in immunohistochemical procedures must evaluate controls and qualify the
the APK Wash (1X) or buffer. Avoid tissue drying. stained product before interpreting results. Staining of negative controls must be noted first,
and these results compared to the stained material to verify that the signal generated is not
BenchMark or BenchMark XT Automated Slide Stainers the cause of nonspecific interactions.
1. Apply slide bar code label which corresponds to the antibody protocol to be
performed. Positive Tissue Control
2. Load the primary antibody and appropriate detection kit dispensers and required The stained positive tissue control should be examined first to ascertain that all reagents
accessory reagent onto the reagent tray and place them on the automated slide are functioning properly. The presence of an appropriately colored reaction product within
stainer. Check bulk fluids and waste. the target cells is indicative of positive reactivity. Refer to the package insert of the
3. Load the slides onto the instrument. detection kit used for expected color reactions. Depending on the incubation length and
potency of the hematoxylin used, counterstaining will result in a pale to dark blue coloration
For All Instruments of cell nuclei. Excessive or incomplete counterstaining may compromise proper
1. Start the staining run. interpretation of results.
2. At the completion of the run, remove the slides from the instrument, and wash in a If the positive tissue control fails to demonstrate positive staining, any results with the test
mild dishwashing detergent or alcohol to remove the coverslip solution; dehydrate, specimens should be considered invalid.
clear, and coverslip with permanent mounting media in the usual manner.
2003-11-30 Page 2 of 4 14910EN Rev A
Negative Tissue Control 2. The detection kit, in combination with Ventana primary antibodies and accessories,
The negative tissue control should be examined after the positive tissue control to verify the detects antigen that survives routine formalin, tissue processing and sectioning.
specific labeling of the target antigen by the primary antibody. The absence of specific 3. Primary antibody incubation time depends on the degree of tissue fixation, and may
staining in the negative tissue control confirms the lack of antibody cross reactivity to cells range from 4 to 32 minutes. For further information on fixation variables, refer to
or cellular components. If specific staining occurs in the negative tissue control, results with Immunomicroscopy: A Diagnostic Tool for the Surgical Pathologist.9
the patient specimen should be considered invalid. 4. This detection kit has been optimized for use with Ventana APK and Reaction Buffer
Nonspecific staining, if present, will have a diffuse appearance. Sporadic light staining of Wash Solutions, Primary Antibodies, Accessories, and the Ventana Automated Slide
connective tissue may also be observed in sections from excessively formalin fixed tissues. Stainers. The use of APK and Reaction Buffer Wash Solution is important to the
Intact cells should be used for interpretation of staining results. Necrotic or degenerated proper function of the detection kit. Users who deviate from recommended test
cells often stain nonspecifically. procedures are responsible for interpretation of patient results under these
circumstances.
Patient Tissue
Patient specimens should be examined last. Positive staining intensity should be assessed SUMMARY OF EXPECTED RESULTS
within the context of any background staining of the negative reagent control. As with any Intra run reproducibility of staining with iVIEWTM DAB Detection Kit was determined by
immunohistochemical test, a negative result means that the antigen in question was not staining ten slides from each of three tissue blocks on the BenchMark, BenchMark XT,
detected, not that the antigen is absent in the cells or tissue assayed. If necessary, use a NexES IHC, and the ES automated slide atainers. The primary antibodies tested were anti-
panel of antibodies to aid in the identification of false negative reactions (see Summary of CD57, anti-CD45RO and anti-PSA. Primary antibody incubation time was 16 minutes.
Expected Results section). The morphology of each tissue sample should also be Slides were counterstained with hematoxylin. The following tissue types were used: tonsil
examined utilizing a hematoxylin and eosin stained section when interpreting any with anti-CD57, tonsil with anti-CD45RO and prostate with anti-PSA. Anti-CD57 was
immunohistochemical result. The patient's morphologic findings and pertinent clinical data pretreated for 4 minutes with Ventana Protease 1, anti-CD57 and anti-CD45RO were not
must be interpreted by a qualified pathologist. pretreated. The staining intensity was scored from 0 to 4+ by a qualified reader. Ten of ten
slides run on the BenchMark, BenchMark XT, NexES IHC and the ES stained positively.
LIMITATIONS Anti-CD57, anti-CD45RO and anti-PSA showed means and standard deviations of 4.0 plus
General Limitations or minus 0.0, 3.95 plus or minus 0.02, and 3.77 plus or minus 0.03 respectively. Users
1. Immunohistochemistry is a multiple step diagnostic process that requires specialized should verify within run reproducibility results by staining several sets of serial sections with
training in the selection of the appropriate reagents, tissue selections, fixation, low, medium and high antigen density in a single run.
processing, preparation of the immunohistochemistry slide, and interpretation of the
staining results. Inter run reproducibility of staining with iVIEW DAB Detection Kit was determined by
2. Tissue staining is dependent on the handling and processing of the tissue prior to staining slides containing sections from the same neutral buffered formalin fixed tissue on
staining. Improper fixation, freezing, thawing, washing, drying, heating, sectioning, or the BenchMark, BenchMark XT, NexES IHC, and the ES Slide Stainers, once a day for
contamination with other tissues or fluids may produce artifacts, antibody trapping, or three days. The primary antibodies tested were anti-CD57, anti-CD45RO and anti-PSA.
false negative results. Inconsistent results may result from variations in fixation and Primary antibody incubation time was 16 minutes. Slides were counterstained with
embedding methods, or from inherent irregularities within the tissue. hematoxylin. The following tissue types were used: tonsil with anti-CD57, tonsil with anti-
3. Excessive or incomplete counterstaining may compromise proper interpretation of CD45RO and prostate with anti-PSA. Anti-CD57 was pretreated for 4 minutes with Ventana
results. Protease 1; anti-CD57 and anti-CD45RO were not pretreated. The staining intensity was
4. The clinical interpretation of any positive staining, or its absence, must be evaluated scored from 0 to 4+ by a qualified reader. The mean and standard deviation of the staining
within the context of clinical history, morphology and other histopathological criteria. intensity between days was 4.0 plus or minus 0.0, 3.99 plus of minus 0.02, and 3.94 plus or
The clinical interpretation of any staining, or its absence, must be complemented by minus 0.07 for CD57, CD45RO, and PSA, respectively. Users should verify between run
morphological studies and proper controls as well as other diagnostic tests. This reproducibility results by staining several sets of serial sections with low, medium, and high
antibody is intended to be used in a panel of antibodies. It is the responsibility of a antigen density on different days.
qualified pathologist to be familiar with the antibodies, reagents and methods used to
produce the stained preparation. Staining must be performed in a certified licensed TROUBLESHOOTING
laboratory under the supervision of a pathologist who is responsible for reviewing the 1. If the positive control exhibits weaker staining than expected, other positive controls
stained slides and assuring the adequacy of positive and negative controls. run during the same instrument run should be checked to determine if it is because of
5. Ventana provides antibodies and reagents at optimal dilution for use when the the primary antibody or one of the common secondary reagents.
provided instructions are followed. Any deviation from recommended test procedures 2. If the positive control is negative, it should be checked to ensure that the slide has the
may invalidate expected results. Appropriate controls must be employed and proper bar code label. If the slide is labeled properly, other positive controls run on
documented. Users who deviate from recommended test procedures must accept the same instrument run should be checked to determine if it is because of the
responsibility for interpretation of patient results. primary antibody or one of the common secondary reagents. Tissues may have been
6. This product is not intended for use in flow cytometry, performance characteristics improperly collected, fixed or deparaffinized. The proper procedure should be
have not been determined. followed for collection, storage and fixation.
7. Reagents may demonstrate unexpected reactions in previously untested tissues. The 3. If excessive background staining occurs, high levels of endogenous biotin may be
possibility of unexpected reactions even in tested tissue groups cannot be completely present. A biotin blocking step should be included (Endogenous Biotin Blocking Kit).
eliminated because of biological variability of antigen expression in neoplasms, or 4. If all of the paraffin has not been removed, the deparaffinization procedure should be
other pathological tissues.5 Contact your local Ventana office with documented repeated.
unexpected reactions. 5. If specific antibody staining is too intense, the run should be repeated with incubation
8. Tissues from persons infected with hepatitis B virus and containing hepatitis B time shortened by 4 minute intervals until the desired stain intensity is achieved.
surface antigen (HBsAg) may exhibit nonspecific staining with horseradish 6. If tissue sections wash off the slide, slides should be checked to ensure that they are
peroxidase.6 positively charged.
9. When used in blocking steps, normal sera from the same animal source as the 7. For corrective action, refer to the Step By Step Procedure section, the automated
secondary antisera may cause false negative or false positive results because of slide stainer Operator’s Manual or contact your local Ventana office.
autoantibodies or natural antibodies.
10. False positive results may be seen because of nonimmunological binding of proteins REFERENCES
or substrate reaction products. They may also be caused by pseudoperoxidase 1. Elias JM, Gown AM, Nakamura RM, Wilbur DC, Herman GE, Jaffe ES, Battifora H,
activity (erythrocytes), endogenous peroxidase activity (cytochrome C), or Brigati DJ. Quality control in immunohistochemistry. Report of a workshop
endogenous biotin (example: liver, brain, breast, kidney) depending on the type of sponsored by the Biological Stain Commission. Am J Clin Pathol 92 (6): 836-843,
immunostain used.7 1989.
2. Sheehan DC, Hrapchak BB. Theory and practice of histotechnology, 2nd Edition.
11. As with any immunohistochemisty test, a negative result means that the antigen was
The C.V. Mosby Company, St. Louis, 1980.
not detected, not that the antigen was absent in the cells or tissue assayed.
3. College of American Pathologists Laboratory Accreditation Program, Anatomic
Pathology Checklist, 2001.
Specific Limitations
4. NCCLS. Quality Assurance for Immunocytochemistry: Approved Guideline. NCCLS
1. Each step of the detection kit procedure has been optimized on the Ventana
document MM4-A- (ISBN 1-56238-396-5). NCCLS, 940 West Valley Road, Suite
automated slide stainers and is preset. Because of variation in tissue fixation and
1400, Wayne, PA 19087-1898 USA, 1999.
processing, it may be necessary to increase or decrease the primary antibody
5. Herman GE, Elfont EA. The taming of immunohistochemistry: the new era of quality
incubation time on individual specimens. For further information on fixation variables,
control. Biotech Histochem 66(4): 194-199, 1991.
refer to “Immunohistochemistry Principles and Advances� .8
2003-11-30 Page 3 of 4 14910EN Rev A
6. Omata M, Liew CT, Ashcavai M, Peters RL. Nonimmunologic binding of horseradish
peroxidase to hepatitis B surface antigen. A possible source of error in
immunohistochemistry. Am J Clin Pathol 73(5): 626-32, 1980.
7. Nadji M, Morales AR. Immunoperoxidase: part 1. The technique and its pitfalls. Lab
Med 14: 767, 1983.
8. Roche PC, Hsi ED. Immunohistochemistry-Principles and Advances. Manual of
Clinical Laboratory Immunology, 6th edition. (NR Rose Ed.) ASM Press, 2002.
9. Hsu SM, Raine L, Fanger H. Use of avidin-biotin-peroxidase complex (ABC) in
immunoperoxidase techniques: A comparison between ABC and unlabeled antibody
(PAP) procedures. J Histochem Cytochem 29: 577-580, 1981.
INTELLECTUAL PROPERTY
CONFIRMTM, EZ PrepTM, iVIEWTM and Liquid CoverslipTM are trademarks of Ventana
Medical Systems, Inc.; BenchMark®, ES®, Gen II®, NexES IHC® and Ventana® are
registered trademarks of Ventana Medical Systems, Inc.
Covered by the following patents: U.S. Pat. Nos. 6045 759, 6192 945 B1, 6416 713 B1 and
foreign counterparts.
CONTACT INFORMATION
North America
Ventana Medical Systems, Inc.
1910 Innovation Park Drive
Tucson, Arizona 85737
U.S.A.
800 227 2155 (U.S. only)
520 229 1910
Europe
Ventana Medical Systems, S.A.
Parc d’Innovation � BP 30 144
Rue G. de Kaysersberg
F - 67404 Illkirch Cedex
France
33 (0) 3 90 40 52 00
EU Representative
MDCI Ltd.
Arundel House
1 Liverpool Gardens
Worthing
West Sussex BN11 1SL
UK
Japan
Ventana Japan K.K.
Landmark Tower, 35F
2-2-1, Minatomirai, Nishi-ku
Yokohama, 220-8135
Japan
81 (0) 45-228-5071
Australia, New Zealand
Ventana Medical Systems, Pty Ltd
5/39 Grand Boulevard
Montmorency VIC 3094
Australia
61 (0) 3 9431 6064
2003-11-30 Page 4 of 4 14910EN Rev A
|