Material Safety Data Sheet (MSDS) for
Proteins 166B Cummings Center s Beverly, MA 01915
Orders s 877-616-CELL (2355) orders@cellsignal.com Support s 877-678-TECH (8324) info@cellsignal.com Web s www.cellsignal.com
Vapor pressure (mm Hg): N/A
I. Identification
Solubility in water: Liquids readily miscible in water
Product/Common name: See list below (used on label)
Phospho-specific antibodies鈥?2000, 3000 and 9000 series
IV. Fire and Explosion Hazard Data
Protein markers鈥?7000 series
Auto-ignition temperature: N/A
Protein kinases, inhibitors and substrates鈥?6000 series
Flash point: N/A
Chemical name: Protein in buffer
Flammable limits in Air (% by volume): N/A
Formula: Complex polypeptides
Percent volatile by volume (%): N/A
Chemical family: Protein
Extinguishing media: Fire extinguishing media should match other
materials in fire.
II. Ingredients Special fire fighting procedures: If involved in fire, don NIOSH/MSHA-
approved self-contained breathing apparatus, flame/chemical resistant.
Material: Hazardous components in water solutions. Buffer components
are listed on product data sheet. Unusual fire and explosion hazards: None known
Principal Hazardous Component CAS
V. Health Hazard Data
Chemical & Common Name Number
(2-[N-morpholinoethane- Threshold Limit Value (TLV) and source: Data not available
sulfonic acid) (MES). . . . . . . . . . #145224-94-8. . . . . . . . . . 0.50 mM
2-mercaptoethanol . . . . . . . . . . . . . . . #60-24-2 . . . . . . . . . . . . . 0鈥?20 mM Chemical listed as carcinogen or potential carcinogen: No
7-amino-4-methylcoumarin . . . . . . . . #26093-31-2 . . . . . . . . . . . 0鈥?1 mM
OSHA permissible exposure limit: None
ATP . . . . . . . . . . . . . . . . . . . . . . . . . . #51963-61-2 . . . . . . . . . 0鈥?0.1 碌M
Brij-35 . . . . . . . . . . . . . . . . . . . . . . . . #9002-92-0 . . . . . . . . . . . . . 0.10% ACGIH Threshold Limit Value: None
Bromophenol blue . . . . . . . . . . . . . . . #62625-28-9 . . . . . . . . . . . . 0鈥?.1%
I.A.R.C. Monographs: No
Caffeine . . . . . . . . . . . . . . . . . . . . . . . #58-08-2 . . . . . . . . . . . . . 0鈥?50 mM
Dithiothreitol (DTT) . . . . . . . . . . . . . . #27565-41-9 . . . . . . . . . . 0鈥?50 mM National Toxicology Program
Dodecyl sodium sulfate . . . . . . . . . . . #151-21-3 . . . . . . . . . . . . . . . 0鈥?6%
2-mercaptoethanol (CAS #60-24-2)鈥擮ral LD50 rat 330 mg/kg; skin
EDTA . . . . . . . . . . . . . . . . . . . . . . . . . #60-00-4 . . . . . . . . . . . . . 0鈥?10 mM
absorption LD50 rat 0.015 ml/kg
EGTA . . . . . . . . . . . . . . . . . . . . . . . . . #67-42-5. . . . . . . . . . . . . . 0.10 mM
Ethylene glycol. . . . . . . . . . . . . . . . . . #107-21-1 . . . . . . . . . . . . . . 0.30% Bromophenol blue (CAS #62625-28-9)鈥擲uspected carcinogen; Handle
Glycerol . . . . . . . . . . . . . . . . . . . . . . . #56-81-5 . . . . . . . . . . . . . . . 0鈥?50% with care!
Imidazole hydrochloride. . . . . . . . . . . #1467-16-9 . . . . . . . . . . 0鈥?250 mM
Caffeine (CAS #58-08-2)鈥擫D50 rat 240 mg/kg
Nonidet P40 . . . . . . . . . . . . . . . . . . . . #9002-93-1 . . . . . . . . . . . . 0鈥?0.1%
Sodium azide . . . . . . . . . . . . . . . . . . . #26628-22-8 . . . . . . . . . . . . . 1 mM EDTA (CAS #60-00-4)鈥擨ntraperitoneal LD50 rat 379 mg/kg; intraperi-
Tris-HCl . . . . . . . . . . . . . . . . . . . . . . . #1185-53-1 . . . . . . . . . . . 0鈥?50 mM toneal LD50 mouse 250 mg/kg
Triton X-100 . . . . . . . . . . . . . . . . . . . . #9036-19-5 . . . . . . . . . . . 0鈥?0.15%
Glycerol (CAS #56-81-5)鈥擮ral LD50 rabbit 27 g/kg
Tween-20 . . . . . . . . . . . . . . . . . . . . . . #9005-64-5 . . . . . . . . . . . . . 0.10%
Complex polypeptides . . . . . . . . . . . . . . . . . . . . . . . . . . . Biological activity
Sodium azide (CAS #26628-22-8)鈥擮ral man 29 mg/kg (LDLO); oral
varies (%) quantity
woman 14 mg/kg (LDLO)
measured by activity
Target Organ Data
III. Physical Data Glycerol (CAS# 56-81-5)鈥擝ehavioral; headache; gastrointestinal; nausea
or vomiting
Boiling point: Decomposes
Acute effects of overexposure: May cause skin irritation. To the best of
Vapor density (Air = 1): N/A our knowledge, the chemical, physical and toxicological properties have
漏 2004 Cell Signaling Technology, Inc.
not been thoroughly investigated. Allergy-prone individuals should be par-
Appearance and odor: Colorless and odorless
ticularly cautious with proteins or other materials of biologic origin. Solu-
Melting point: N/A tions containing sodium azide (CAS #26628-22-8) may be fatal if initially
swallowed or absorbed through skin.
Freezing Point: N/A
Swallowing: May be harmful if swallowed.
Vapor Pressure at 20掳C: N/A
Skin absorption: May be harmful if absorbed through the skin.
Specific gravity (H2O = 1): Varies
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Evaporation rate: N/A
Material Safety Data Sheet (MSDS) for
Proteins 166B Cummings Center s Beverly, MA 01915
Orders s 877-616-CELL (2355) orders@cellsignal.com Support s 877-678-TECH (8324) info@cellsignal.com Web s www.cellsignal.com
Inhalation: May be harmful if inhaled. Material may be irritating to
IX. Special Precautions
mucous membranes and upper respiratory tract.
Precautions to be taken in handling and storage: This compound is
Skin contact: May be harmful.
sold only for research use by personnel familiar with the toxicology of
Eye contact: May cause eye irritation. organic chemicals and who are well trained in good laboratory habits, such
as avoiding spills, keeping hands clean at all times and not rubbing eyes
Chronic effects of Overexposure: May be harmful. Allergic reactions in
with hands while working in the laboratory.
certain sensitive individuals may develop.
This compound is sold only in milligram quantities for use in dilute solu-
Emergency and First Aid Procedures
tions in biological research. No other use is intended, and any other use
Swallowing鈥擶ash out mouth with water, provided person is conscious.
may involve substantial hazards.
Call a physician.
The above information is believed to be correct but does not purport to be
Skin鈥擨n case of contact, immediately wash skin with soap and copious
all inclusive and shall be used only as a guide for experienced personnel.
amounts of water.
Cell Signaling Technology, Inc., shall not be held liable for any damage
resulting from the handling of or from contact with the above product. The
Inhalation鈥擨f inhaled, remove to fresh air. If breathing becomes difficult,
burden of safe use of this material rests entirely with the user.
call a physician.
Eyes鈥擨n case of contact with eyes, flush with copious amounts of water
for at least 15 minutes. Assure adequate flushing by separating eyelids with
Revised: August 2001
fingers. Consult a physician.
VI. Reactivity Data
Stability: Any degradation products are not hazardous.
Stability/Conditions to avoid: Not reactive as far as is known
Incompatibility/Materials to avoid: None known
Hazardous Decomposition Products: None known
Hazardous polymerization: Will not occur
VII. Spill or Leak Procedures
Steps to be taken if material is spilled or released: Wear lab coat,
chemical resistant gloves and safety glasses. Wipe up spill with a suitable
absorbant and dispose of properly. Wash down the spill site.
Waste disposal method: Dispose of in accordance with all federal, state
and local regulations.
VIII. Special Protection Information
Respiratory protection: Not required. Use mechanical exhaust.
Ventilation: Use mechanical exhaust.
Protective equipment: Good laboratory practices should always be
used. Wear suitable protective clothing, chemical resistant gloves and lab
safety glasses.
漏 2004 Cell Signaling Technology, Inc.
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