SIGMA-ALDRICH
Material Safety Data Sheet
Date Printed: 13/APR/2005
Date Updated: 10/FEB/2005
Version 1.4
According to 91/155/EEC
Classified as Hazardous according to the criteria of EU Annex 1 and
NOHSC.
1 - Product and Company Information
Product Name CHLOROFORM: ISOAMYL ALCOHOL 24:1
MOLECULAR BIOLOGY REAGENT
Product Number C0549
Company Sigma-Aldrich Pty, Ltd
Unit 2, 14 Anella Avenue
Castle Hill NSW 1765
Australia
Technical Phone # +61 2 9841 0555
Fax +61 2 9841 0500
Emergency Phone # +61 2 9841 0566
2 - Composition/Information on Ingredients
Product Name CAS # EC no Annex I
Index Number
CHLOROFORM:ISOAMYL ALCOHOL 24:1 None None None
Ingredient Name Percent CAS # EC no Annex I
Index Number
CHLOROFORM 96 67-66-3 200-663-8 602-006-00-4
Carc. Cat.3
Symbols: Xn
R-Phrases: 22 38 40 48/20/22
Harmful if swallowed. Irritating to skin. Limited evidence of a
carcinogenic effect. Harmful: danger of serious damage to health
by prolonged exposure through inhalation and if swallowed.
3-METHYL-1-BUTANOL 4 123-51-3 204-633-5 603-006-00-7
Symbols: Xn
R-Phrases: 10 20
Flammable. Harmful by inhalation.
3 - Hazards Identification
SPECIAL INDICATION OF HAZARDS TO HUMANS AND THE ENVIRONMENT
Harmful if swallowed. Irritating to skin. Limited evidence of a
carcinogenic effect. Harmful: danger of serious damage to health
by prolonged exposure through inhalation and if swallowed.
4 - First Aid Measures
AFTER INHALATION
If inhaled, remove to fresh air. If breathing becomes difficult,
call a physician.
AFTER SKIN CONTACT
In case of contact, immediately wash skin with soap and copious
amounts of water.
AFTER EYE CONTACT
In case of contact with eyes, flush with copious amounts of
water for at least 15 minutes. Assure adequate flushing by
separating the eyelids with fingers. Call a physician.
AFTER INGESTION
If swallowed, wash out mouth with water provided person is
conscious. Call a physician.
GENERAL INFORMATION
Contamination of the eyes should be treated by immediate and
prolonged irrigation with copious amounts of water.
5 - Fire Fighting Measures
EXTINGUISHING MEDIA
Suitable: Noncombustible. Use extinguishing media appropriate to
surrounding fire conditions.
SPECIAL RISKS
Specific Hazard(s): Emits toxic fumes under fire conditions.
SPECIAL PROTECTIVE EQUIPMENT FOR FIREFIGHTERS
Wear self-contained breathing apparatus and protective clothing
to prevent contact with skin and eyes.
6 - Accidental Release Measures
PERSONAL PRECAUTION PROCEDURES TO BE FOLLOWED IN CASE OF LEAK OR SPILL
Evacuate area.
PROCEDURE(S) OF PERSONAL PRECAUTION(S)
Wear self-contained breathing apparatus, rubber boots, and heavy
rubber gloves. Wear disposable coveralls and discard them after
use.
METHODS FOR CLEANING UP
Absorb on sand or vermiculite and place in closed containers for
disposal. Ventilate area and wash spill site after material
pickup is complete.
7 - Handling and Storage
HANDLING
Directions for Safe Handling: Do not breathe vapor. Do not get
in eyes, on skin, on clothing. Avoid prolonged or repeated
exposure.
STORAGE
Conditions of Storage: Keep tightly closed.
SPECIAL REQUIREMENTS: Light sensitive.
8 - Exposure Controls / Personal Protection
ENGINEERING CONTROLS
Use only in a chemical fume hood. Safety shower and eye bath.
GENERAL HYGIENE MEASURES
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Wash contaminated clothing before reuse. Wash thoroughly after
handling.
EXPOSURE LIMITS
Country Source Type Value
Poland NDS 50 MG/M3
Poland NDSCh 225 MG/M3
Poland NDSP -
EXPOSURE LIMITS - EUROPEAN UNION
Source Type Value
OEL OEL 10 mg/m3
2 ppm
Remarks: Skin
EXPOSURE LIMITS - DENMARK
Source Type Value
OEL TWA 10 mg/m3
2 ppm
Remarks: K
EXPOSURE LIMITS - GERMANY
Source Type Value
TRGS 900 OEL 50 mg/m3
10 ppm
Remarks: 4
EXPOSURE LIMITS - NORWAY
Source Type Value
OEL 10 mg/m3
2 ppm
Remarks: HKR
EXPOSURE LIMITS - SWEDEN
Source Type Value
LLV (Level10 mg/m3
2 ppm
Remarks: K
EXPOSURE LIMITS - SWITZERLAND
Source Type Value
OEL OEL 50 mg/m3
10 ppm
Remarks: B
EXPOSURE LIMITS - UNITED KINGDOM
Source Type Value
OEL OEL 9.9 mg/m3
2 ppm
PERSONAL PROTECTIVE EQUIPMENT
Respiratory Protection: Government approved respirator in
nonventilated areas and/or for exposure above the TLV or PEL.
Hand Protection: Compatible chemical-resistant gloves.
Eye Protection: Chemical safety goggles.
9 - Physical and Chemical Properties
Appearance Physical State: Liquid
Property Value At Temperature or Pressure
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pH N/A
BP/BP Range N/A
MP/MP Range N/A
Flash Point N/A
Flammability N/A
Autoignition Temp N/A
Oxidizing Properties N/A
Explosive Properties N/A
Explosion Limits N/A
Vapor Pressure N/A
SG/Density N/A
Partition Coefficient N/A
Viscosity N/A
Vapor Density N/A
Saturated Vapor Conc. N/A
Evaporation Rate N/A
Bulk Density N/A
Decomposition Temp. N/A
Solvent Content N/A
Water Content N/A
Surface Tension N/A
Conductivity N/A
Miscellaneous Data N/A
Solubility N/A
10 - Stability and Reactivity
STABILITY
Stable: Stable.
Conditions of Instability: Light sensitive.
Materials to Avoid: Strong oxidizing agents, Strong bases,
Magnesium, Sodium, Lithium.
HAZARDOUS DECOMPOSITION PRODUCTS
Hazardous Decomposition Products: Carbon monoxide, Carbon dioxide,
Phosgene gas, Chlorine.
HAZARDOUS POLYMERIZATION
Hazardous Polymerization: Will not occur
11 - Toxicological Information
SIGNS AND SYMPTOMS OF EXPOSURE
Exposure can cause: Vomiting. Gastrointestinal disturbances.
Exposure to and/or consumption of alcohol may increase toxic
effects. To the best of our knowledge, the chemical, physical,
and toxicological properties have not been thoroughly
investigated.
ROUTE OF EXPOSURE
Skin Contact: Causes skin irritation.
Skin Absorption: May be harmful if absorbed through the skin.
Skin absorption may occur.
Eye Contact: Causes eye irritation.
Inhalation: May be harmful if inhaled. Material may be
irritating to mucous membranes and upper respiratory tract.
Ingestion: Harmful if swallowed.
TARGET ORGAN INFORMATION
Cardiovascular system. Central nervous system. Blood. Liver.
Kidneys.
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CONDITIONS AGGRAVATED BY EXPOSURE
May cause nervous system disturbances.
CHRONIC EXPOSURE - CARCINOGEN
Result: This product is or contains a component that has been
reported to be probably carcinogenic based on its IARC, OSHA,
ACGIH, NTP, or EPA classification. The National Cancer Institute
(NCI) has found clear evidence for carcinogenicity.
IARC CARCINOGEN LIST
Rating: Group 2B Group 2B
CHRONIC EXPOSURE - MUTAGEN
Result: Laboratory experiments have shown mutagenic effects.
CMR CAT.: Carc. Cat.3
12 - Ecological Information
No data available.
ECOTOXICOLOGICAL EFFECTS
Test Type: EC50 Algae
Time: 24 h
Value: 500 mg/l
Test Type: EC50 Daphnia
Species: Daphnia magna
Time: 24 h
Value: 79 mg/l
Test Type: LC50 Fish
Species: Leuciscus idus
Time: 48 h
Value: 162 mg/l
Test Type: LC100 Fish
Species: Leuciscus idus
Time: 48 h
Value: 220 mg/l
Test Type: LC50 Fish
Time: 4 days
Value: 97 mg/l
Test Type: LC50 Fish
Species: Brachydanio rerio
Time: 96 h
Value: 121 mg/l
13 - Disposal Considerations
SUBSTANCE DISPOSAL
Contact a licensed professional waste disposal service to dispose
of this material. Observe all federal, state, and local
environmental regulations.
14 - Transport Information
RID/ADR
UN#: 1888
Class: 6.1
PG: III
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Proper Shipping Name: Chloroform solution
IMDG
UN#: 1888
Class: 6.1
PG: III
Proper Shipping Name: Chloroform solution
Marine Pollutant: No
Severe Marine Pollutant: No
IATA
UN#: 1888
Class: 6.1
PG: III
Proper Shipping Name: Chloroform solution
Inhalation Packing Group I: No
15 - Regulatory Information
CLASSIFICATION AND LABELING ACCORDING TO EU DIRECTIVES
INDICATION OF DANGER: Xn
Harmful.
R-PHRASES: 22 38 40 48/20/22
Harmful if swallowed. Irritating to skin. Limited evidence of a
carcinogenic effect. Harmful: danger of serious damage to
health by prolonged exposure through inhalation and if
swallowed.
S-PHRASES: 36/37
Wear suitable protective clothing and gloves.
COUNTRY SPECIFIC INFORMATION
Germany
WGK: 3
SWITZERLAND
SWISS POISON CLASS: 1*
NORWAY
Labelling for organic solvents where the package is 1liter or
more.
YL-tall m3/l: 294000
YL-group: 5
Safety phrases: 38 42 210
In case of insufficient ventilation, wear suitable respiratory
equipment. During fumigation/spraying wear suitable respiratory
equipment. Use compressed air- or fresh air line breathing
apparatus in confined spaces.
Declaration Number: 1933
16 - Other Information
WARRANTY
The above information is believed to be correct but does not
purport to be all inclusive and shall be used only as a guide. The
information in this document is based on the present state of our
knowledge and is applicable to the product with regard to
appropriate safety precautions. It does not represent any
guarantee of the properties of the product. Sigma-Aldrich Inc.,
shall not be held liable for any damage resulting from handling or
from contact with the above product. See reverse side of invoice
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or packing slip for additional terms and conditions of sale.
Copyright 2005 Sigma-Aldrich Co. License granted to make unlimited
paper copies for internal use only.
DISCLAIMER
For R&D use only. Not for drug, household or other uses.
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