SIGMA-ALDRICH
Material Safety Data Sheet
Date Printed: 03/JUL/2005
Date Updated: 02/JUL/2005
Version 1.4
According to 91/155/EEC
Classified as Hazardous according to the criteria of EU Annex 1 and
NOHSC.
1 - Product and Company Information
Product Name CHLORAMBUCIL
Product Number C0253
Company Sigma-Aldrich Pty. Ltd.
12 Anella Avenue
Castle Hill NSW 2154
Australia
Technical Phone # +61 2 9841 0555 (1800 800 097)
Fax +61 2 9841 0500 (1800 800 096)
Emergency Phone # +44 8701906777 (1800 448 465)
2 - Composition/Information on Ingredients
Product Name CAS # EC no Annex I
Index Number
CHLORAMBUCIL 305-03-3 206-162-0 None
Formula C14H19Cl2NO2
Molecular Weight 304.2 AMU
Synonyms Ambochlorin * Amboclorin * Benzenebutanoic acid,
4-(bis(2-chloroethyl)amino) *
4-(Bis(2-chloroethyl)amino)benzenebutanoic acid
* gamma-(p-Bis(2-chloroethyl)aminophenyl)butyric
acid *
4-(p-(Bis(2-chloroethyl)amino)phenyl)butyric
acid *
4(p-Bis(beta-chloroethyl)aminophenyl)butyric
acid * Butanoic acid,
4-(bis(2-chloroethyl)amino)benzene * CB 1348 *
Chlorambucil * Chloraminophen * Chloraminophene
* Chlorobutin * Chlorobutine *
N,N-Di-2-chloroethyl-gamma-p-aminophenylbutyric
acid * p-(N,N-Di-2-chloroethyl)aminophenyl
butyric acid *
p-N,N-Di-(beta-chloroethyl)aminophenyl butyric
acid *
gamma-(p-Di(2-chloroethyl)aminophenyl)butyric
aci * Ecloril * Kyselina
4-(N,N-bis-(2-chlorethyl)-p-aminofenyl)maselna
(Czech) * Leukeran * Linfolizin * Linfolysin *
Lympholysin * NCI-C03485 * NSC-3088 *
Phenylbuttersaeure-lost (German) * Phenylbutyric
3 - Hazards Identification
SPECIAL INDICATION OF HAZARDS TO HUMANS AND THE ENVIRONMENT
May cause cancer. Toxic if swallowed. Irritating to eyes,
respiratory system and skin.
4 - First Aid Measures
AFTER INHALATION
If inhaled, remove to fresh air. If not breathing give
artificial respiration. If breathing is difficult, give oxygen.
AFTER SKIN CONTACT
In case of skin contact, flush with copious amounts of water for
at least 15 minutes. Remove contaminated clothing and shoes.
Call a physician.
AFTER EYE CONTACT
In case of contact with eyes, flush with copious amounts of
water for at least 15 minutes. Assure adequate flushing by
separating the eyelids with fingers. Call a physician.
AFTER INGESTION
If swallowed, wash out mouth with water provided person is
conscious. Call a physician immediately.
5 - Fire Fighting Measures
EXTINGUISHING MEDIA
Suitable: Water spray. Carbon dioxide, dry chemical powder, or
appropriate foam.
SPECIAL RISKS
Specific Hazard(s): Emits toxic fumes under fire conditions.
SPECIAL PROTECTIVE EQUIPMENT FOR FIREFIGHTERS
Wear self-contained breathing apparatus and protective clothing
to prevent contact with skin and eyes.
6 - Accidental Release Measures
PERSONAL PRECAUTION PROCEDURES TO BE FOLLOWED IN CASE OF LEAK OR SPILL
Evacuate area.
PROCEDURE(S) OF PERSONAL PRECAUTION(S)
Wear self-contained breathing apparatus, rubber boots, and heavy
rubber gloves.
METHODS FOR CLEANING UP
Sweep up, place in a bag and hold for waste disposal. Avoid
raising dust. Ventilate area and wash spill site after material
pickup is complete.
7 - Handling and Storage
HANDLING
Directions for Safe Handling: Do not breathe dust. Do not get in
eyes, on skin, on clothing. Avoid prolonged or repeated exposure.
STORAGE
Conditions of Storage: Keep tightly closed.
Store at 2-8掳C
8 - Exposure Controls / Personal Protection
ENGINEERING CONTROLS
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Use only in a chemical fume hood. Safety shower and eye bath.
GENERAL HYGIENE MEASURES
Wash contaminated clothing before reuse. Wash thoroughly after
handling.
PERSONAL PROTECTIVE EQUIPMENT
Respiratory Protection: Government approved respirator.
Hand Protection: Compatible chemical-resistant gloves.
Eye Protection: Chemical safety goggles.
9 - Physical and Chemical Properties
Appearance Physical State: Solid
Color: Slightly beige
Form: Powder
Property Value At Temperature or Pressure
pH N/A
BP/BP Range N/A
MP/MP Range 64 掳C
Flash Point N/A
Flammability N/A
Autoignition Temp N/A
Oxidizing Properties N/A
Explosive Properties N/A
Explosion Limits N/A
Vapor Pressure N/A
SG/Density N/A
Partition Coefficient Log Kow: 1.47
Viscosity N/A
Vapor Density N/A
Saturated Vapor Conc. N/A
Evaporation Rate N/A
Bulk Density N/A
Decomposition Temp. N/A
Solvent Content N/A
Water Content N/A
Surface Tension N/A
Conductivity N/A
Miscellaneous Data N/A
Solubility N/A
10 - Stability and Reactivity
STABILITY
Stable: Stable.
Conditions of Instability: Light.
HAZARDOUS DECOMPOSITION PRODUCTS
Hazardous Decomposition Products: Carbon monoxide, Carbon dioxide,
Nitrogen oxides, Hydrogen chloride gas.
HAZARDOUS POLYMERIZATION
Hazardous Polymerization: Will not occur
11 - Toxicological Information
RTECS NUMBER: ES7525000
ACUTE TOXICITY
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LD50
Oral
Rat
76 mg/kg
LD50
Intraperitoneal
Rat
14 MG/KG
LD50
Intracerebral
Rat
>400 UG/KG
LD50
Oral
Mouse
80 mg/kg
LD50
Intraperitoneal
Mouse
20 MG/KG
LD50
Subcutaneous
Mouse
26 MG/KG
SIGNS AND SYMPTOMS OF EXPOSURE
Exposure can cause drug fever, myelosuppression, hepatotoxicity,
infertility, seizures, and G.I. toxicity.
ROUTE OF EXPOSURE
Skin Contact: Causes skin irritation.
Skin Absorption: May be harmful if absorbed through the skin.
Eye Contact: Causes eye irritation.
Inhalation: May be harmful if inhaled. Material is irritating to
mucous membranes and upper respiratory tract.
Ingestion: Toxic if swallowed.
TARGET ORGAN INFORMATION
Bone marrow.
CHRONIC EXPOSURE - CARCINOGEN
Result: This is or contains a component that has been reported
to be carcinogenic based on its IARC, OSHA, ACGIH, NTP, or EPA
classification.
Man
Route of Application: Oral
Exposure Time: 2.5Y
Result: Tumorigenic:Carcinogenic by RTECS criteria.
Blood:Leukemia
Woman
Route of Application: Oral
Exposure Time: 82W
Result: Tumorigenic:Carcinogenic by RTECS criteria.
Blood:Leukemia
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Woman
Route of Application: Unreported
Exposure Time: 3Y
Result: Tumorigenic:Carcinogenic by RTECS criteria.
Blood:Leukemia
Rat
Route of Application: Intraperitoneal
Exposure Time: 26W
Result: Tumorigenic:Carcinogenic by RTECS criteria.
Blood:Leukemia Blood:Lymphomas including Hodgkin's disease.
Mouse
Route of Application: Skin
Exposure Time: 9W
Result: Tumorigenic:Neoplastic by RTECS criteria. Lungs, Thorax,
or Respiration:Tumors. Skin and Appendages: Other: Tumors.
Mouse
Route of Application: Intraperitoneal
Exposure Time: 4W
Result: Tumorigenic:Carcinogenic by RTECS criteria. Lungs,
Thorax, or Respiration:Tumors.
Rat
Route of Application: Intraperitoneal
Exposure Time: 26W
Result: Tumorigenic:Carcinogenic by RTECS criteria.
Blood:Leukemia Blood:Lymphomas including Hodgkin's disease.
Man
Route of Application: Oral
Exposure Time: 96W
Result: Tumorigenic:Carcinogenic by RTECS criteria.
Blood:Leukemia
Woman
Route of Application: Oral
Exposure Time: 6Y
Result: Tumorigenic:Carcinogenic by RTECS criteria.
Blood:Leukemia
Woman
Route of Application: Oral
Exposure Time: 7Y
Result: Tumorigenic:Carcinogenic by RTECS criteria.
Blood:Leukemia
Human
Route of Application: Oral
Exposure Time: 5Y
Result: Tumorigenic:Carcinogenic by RTECS criteria.
Blood:Leukemia
Woman
Route of Application: Oral
Exposure Time: 3Y
Result: Tumorigenic:Carcinogenic by RTECS criteria.
Blood:Leukemia
Woman
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Route of Application: Oral
Exposure Time: 4Y
Result: Tumorigenic:Carcinogenic by RTECS criteria.
Blood:Leukemia
Human
Route of Application: Oral
Exposure Time: 3Y
Result: Tumorigenic:Carcinogenic by RTECS criteria.
Blood:Leukemia
Woman
Route of Application: Oral
Exposure Time: 94W
Result: Tumorigenic:Carcinogenic by RTECS criteria.
Blood:Leukemia
Child
Route of Application: Oral
Exposure Time: 77W
Result: Tumorigenic:Carcinogenic by RTECS criteria.
Blood:Leukemia
IARC CARCINOGEN LIST
Rating: Group 1
CHRONIC EXPOSURE - MUTAGEN
Human
40 UMOL/L
Cell Type: lymphocyte
DNA damage
Human
1 UMOL/L
Cell Type: lymphocyte
Unscheduled DNA synthesis
Human
5 MG/L
Cell Type: HeLa cell
Other mutation test systems
Human
15 UMOL/L
Cell Type: HeLa cell
DNA inhibition
Human
3 MG/KG
Unreported
8W
Cytogenetic analysis
Human
250 UG/L
Cell Type: lymphocyte
Cytogenetic analysis
Human
8800 UG/KG
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Unreported
44D
Sister chromatid exchange
Human
200 NMOL/L
Cell Type: lymphocyte
Sister chromatid exchange
Human
225 UG/L
Cell Type: lymphocyte
Sister chromatid exchange
Rat
12 MG/KG
Intraperitoneal
DNA
Rat
12 MG/KG
Intraperitoneal
Other mutation test systems
Rat
19 MG/KG
Oral
DNA inhibition
Rat
3300 NMOL/L
Cell Type: Other cell types
Unscheduled DNA synthesis
Rat
10 MG/KG
Intraperitoneal
Other mutation test systems
Rat
55 MG/KG
Oral
Other mutation test systems
Rat
10 MG/KG
Intraperitoneal
Cytogenetic analysis
Rat
55 MG/KG
Oral
Cytogenetic analysis
Mouse
4 MG/KG
Intraperitoneal
Micronucleus test
Mouse
10 MG/KG
Intraperitoneal
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specific locus test
Mouse
20 MG/KG
Intraperitoneal
DNA damage
Mouse
20 UMOL/L
Cell Type: Ascites tumor
DNA
Mouse
1 PPH
Skin
DNA inhibition
Mouse
5000 PPM
Intravaginal
DNA inhibition
Mouse
15 MG/L
Cell Type: Embryo
DNA inhibition
Mouse
6 MG/KG
Intraperitoneal
Dominant lethal test
Mouse
365 UG/KG
Cell Type: Ascites tumor
Host-mediated assay
Mouse
6 MG/KG
Intraperitoneal
Heritable translocation test
Hamster
80 UG/L
Cell Type: kidney
Morphological transformation.
Hamster
2 UMOL/L
Cell Type: lung
Cytogenetic analysis
Hamster
200 NMOL/L
Cell Type: lung
Sister chromatid exchange
Hamster
75 UG/L
Cell Type: ovary
Sister chromatid exchange
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Hamster
10 UMOL/L
Cell Type: lung
Mutation in mammalian somatic cells.
Hamster
20 MG/L
Cell Type: ovary
Mutation in mammalian somatic cells.
Mammal
2500 NMOL/L
Cell Type: lymphocyte
DNA damage
CHRONIC EXPOSURE - TERATOGEN
Species: Woman
Dose: 5160 UG/KG
Route of Application: Oral
Exposure Time: (33-75D PREG)
Result: Specific Developmental Abnormalities: Urogenital system.
Species: Rat
Dose: 8 MG/KG
Route of Application: Oral
Exposure Time: (15D PREG)
Result: Specific Developmental Abnormalities: Central nervous
system. Specific Developmental Abnormalities: Eye, ear.
Species: Rat
Dose: 6 MG/KG
Route of Application: Oral
Exposure Time: (11D PREG)
Result: Specific Developmental Abnormalities: Musculoskeletal
system.
Species: Rat
Dose: 10 MG/KG
Route of Application: Oral
Exposure Time: (15D PREG)
Result: Effects on Embryo or Fetus: Fetal death.
Species: Rat
Dose: 12 MG/KG
Route of Application: Intraperitoneal
Exposure Time: (13D PREG)
Result: Effects on Embryo or Fetus: Fetal death.
Species: Rat
Dose: 6 MG/KG
Route of Application: Intraperitoneal
Exposure Time: (13D PREG)
Result: Specific Developmental Abnormalities: Central nervous
system. Specific Developmental Abnormalities: Craniofacial
(including nose and tongue).
Species: Rat
Dose: 4 MG/KG
Route of Application: Intraperitoneal
Exposure Time: (13D PREG)
Result: Specific Developmental Abnormalities: Craniofacial
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(including nose and tongue). Specific Developmental
Abnormalities: Musculoskeletal system.
Species: Rat
Dose: 6 MG/KG
Route of Application: Intraperitoneal
Exposure Time: (10D PREG)
Result: Specific Developmental Abnormalities: Urogenital system.
Species: Rat
Dose: 6 MG/KG
Route of Application: Intraperitoneal
Exposure Time: (12D PREG)
Result: Effects on Embryo or Fetus: Extra embryonic structures
(e.g., placenta, umbilical cord). Effects on Embryo or Fetus:
Fetotoxicity (except death, e.g., stunted fetus).
Species: Rat
Dose: 8 MG/KG
Route of Application: Parenteral
Exposure Time: (13D PREG)
Result: Effects on Embryo or Fetus: Cytological changes
(including somatic cell genetic material).
Species: Mouse
Dose: 3 MG/KG
Route of Application: Oral
Exposure Time: (7D PREG)
Result: Effects on Embryo or Fetus: Cytological changes
(including somatic cell genetic material). Specific
Developmental Abnormalities: Central nervous system.
Species: Mouse
Dose: 4 MG/KG
Route of Application: Oral
Exposure Time: (9D PREG)
Result: Effects on Embryo or Fetus: Fetotoxicity (except death,
e.g., stunted fetus). Specific Developmental Abnormalities:
Central nervous system. Specific Developmental Abnormalities:
Musculoskeletal system.
Species: Mouse
Dose: 7 MG/KG
Route of Application: Intraperitoneal
Exposure Time: (11D PREG)
Result: Specific Developmental Abnormalities: Craniofacial
(including nose and tongue). Specific Developmental
Abnormalities: Musculoskeletal system.
Species: Mouse
Dose: 5 MG/KG
Route of Application: Subcutaneous
Exposure Time: (10D PREG)
Result: Effects on Embryo or Fetus: Fetotoxicity (except death,
e.g., stunted fetus). Specific Developmental Abnormalities:
Cardiovascular (circulatory) system. Specific Developmental
Abnormalities: Urogenital system.
Species: Hamster
Dose: 13500 UG/KG
Route of Application: Intraperitoneal
Exposure Time: (8D PREG)
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Result: Specific Developmental Abnormalities: Other
developmental abnormalities.
CHRONIC EXPOSURE - REPRODUCTIVE HAZARD
Species: Woman
Dose: 13 MG/KG
Route of Application: Oral
Exposure Time: (56D PRE)
Result: Maternal Effects: Menstrual cycle changes or disorders.
Species: Man
Dose: 143 UG/KG
Route of Application: Unreported
Exposure Time: (6D MALE)
Result: Paternal Effects: Spermatogenesis (including genetic
material, sperm morphology,motility, and count).
Species: Man
Dose: 1 MG/KG
Route of Application: Unreported
Exposure Time: (55W MALE)
Result: Paternal Effects: Spermatogenesis (including genetic
material, sperm morphology,motility, and count).
Species: Man
Dose: 5857 UG/KG
Route of Application: Unreported
Exposure Time: (30W MALE)
Result: Paternal Effects: Spermatogenesis (including genetic
material, sperm morphology,motility, and count).
Species: Rat
Dose: 8 MG/KG
Route of Application: Oral
Exposure Time: (11D PREG)
Result: Effects on Fertility: Post-implantation mortality (e.g.,
dead and/or resorbed implants per total number of implants).
Species: Rat
Dose: 3 MG/KG
Route of Application: Intraperitoneal
Exposure Time: (3D PREG)
Result: Effects on Fertility: Post-implantation mortality (e.g.,
dead and/or resorbed implants per total number of implants).
Effects on Embryo or Fetus: Cytological changes (including
somatic cell genetic material).
Species: Mouse
Dose: 14200 UG/KG
Route of Application: Oral
Exposure Time: (11D PREG)
Result: Effects on Fertility: Post-implantation mortality (e.g.,
dead and/or resorbed implants per total number of implants).
Specific Developmental Abnormalities: Central nervous system.
Specific Developmental Abnormalities: Musculoskeletal system.
Species: Mouse
Dose: 80 MG/KG
Route of Application: Oral
Exposure Time: (8-12D PREG)
Result: Effects on Newborn: Live birth index (# fetuses per
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litter; measured after birth).
Species: Mouse
Dose: 4 MG/KG
Route of Application: Intraperitoneal
Exposure Time: (1D MALE)
Result: Paternal Effects: Spermatogenesis (including genetic
material, sperm morphology,motility, and count).
12 - Ecological Information
No data available.
13 - Disposal Considerations
SUBSTANCE DISPOSAL
Contact a licensed professional waste disposal service to dispose
of this material. Dissolve or mix the material with a combustible
solvent and burn in a chemical incinerator equipped with an
afterburner and scrubber. Observe all federal, state, and local
environmental regulations.
14 - Transport Information
RID/ADR
UN#: 2811
Class: 6.1
PG: III
Proper Shipping Name: Toxic solid, organic, n.o.s.
IMDG
UN#: 2811
Class: 6.1
PG: III
Proper Shipping Name: Toxic solid, organic, n.o.s.
Marine Pollutant: No
Severe Marine Pollutant: No
Technical Name: Required
IATA
UN#: 2811
Class: 6.1
PG: III
Proper Shipping Name: Toxic solid, organic, n.o.s.
Inhalation Packing Group I: No
Technical Name: Required
15 - Regulatory Information
CLASSIFICATION AND LABELING ACCORDING TO EU DIRECTIVES
INDICATION OF DANGER: T
Toxic.
R-PHRASES: 45 25 36/37/38
May cause cancer. Toxic if swallowed. Irritating to eyes,
respiratory system and skin.
S-PHRASES: 53 26 45
Avoid exposure - obtain special instructions before use. In
case of contact with eyes, rinse immediately with plenty of
water and seek medical advice. In case of accident or if you
feel unwell, seek medical advice immediately (show the label
where possible).
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COUNTRY SPECIFIC INFORMATION
Germany
WGK: 3 (Self classification)
16 - Other Information
WARRANTY
The above information is believed to be correct but does not
purport to be all inclusive and shall be used only as a guide. The
information in this document is based on the present state of our
knowledge and is applicable to the product with regard to
appropriate safety precautions. It does not represent any
guarantee of the properties of the product. Sigma-Aldrich Inc.,
shall not be held liable for any damage resulting from handling or
from contact with the above product. See reverse side of invoice
or packing slip for additional terms and conditions of sale.
Copyright 2005 Sigma-Aldrich Co. License granted to make unlimited
paper copies for internal use only.
DISCLAIMER
For R&D use only. Not for drug, household or other uses.
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