SIGMA-ALDRICH
Material Safety Data Sheet
Date Printed: 31/MAR/2005
Date Updated: 26/JAN/2005
Version 1.5
According to 91/155/EEC
Classified as Hazardous according to the criteria of EU Annex 1 and
NOHSC.
1 - Product and Company Information
Product Name CHLORAMPHENICOL CRYSTALLINE
Product Number C0378
Company Sigma-Aldrich Pty, Ltd
Unit 2, 14 Anella Avenue
Castle Hill NSW 1765
Australia
Technical Phone # +61 2 9841 0555
Fax +61 2 9841 0500
Emergency Phone # +61 2 9841 0566
2 - Composition/Information on Ingredients
Product Name CAS # EC no Annex I
Index Number
CHLORAMPHENICOL 56-75-7 200-287-4 None
Formula C11H12Cl2N2O5
Molecular Weight 323.13 AMU
Synonyms Acetamide,
2,2-dichloro-N-(beta-hydroxy-alpha-(hydroxymethyl
)-p-nitrophenethyl)- * Acetamide,
2,2-dichloro-N-(2-hydroxy-1-(hydroxymethyl)-2-(4-
nitrophenyl)ethyl)-, (R-(R*,R*))- * Alficetyn *
Ambofen * Amphenicol * Amphicol * Amseclor *
Anacetin * Aquamycetin * Austracol * Biocetin *
Biophenicol * CAF (pharmaceutical) * CAM * CAP *
Catilan * Chemicetin * Chemicetina * Chlomin *
Chlomycol * Chloramex * Chloramfenikol (Czech) *
Chloramphenicol * D-Chloramphenicol *
D-(-)-threo-Chloramphenicol * Chloramsaar *
Chlorasol * Chlora-tabs * Chloricol *
Chlornitromycin * Chloroamphenicol * Chlorocaps
* Chlorocid * Chlorocide * Chlorocidin C *
Chlorocidin C tetran * Chlorocid S * Chlorocol *
Chloroject L * Chloromax * Chloromycetin *
Chloromycetny (Polish) * Chloronitrin *
Chloroptic * Chloro-25 vetag * Chlorovules *
Cidocetine * Ciplamycetin * Cloramficin *
Cloramicol * Cloramidina * Cloroamfenicolo
(Italian) * Clorocyn * Cloromisan * Clorosintex
* Comycetin * CPh * Cylphenicol * Desphen *
3 - Hazards Identification
SPECIAL INDICATION OF HAZARDS TO HUMANS AND THE ENVIRONMENT
May cause cancer.
4 - First Aid Measures
AFTER INHALATION
If inhaled, remove to fresh air. If breathing becomes difficult,
call a physician.
AFTER SKIN CONTACT
In case of contact, immediately wash skin with soap and copious
amounts of water.
AFTER EYE CONTACT
In case of contact with eyes, flush with copious amounts of
water for at least 15 minutes. Assure adequate flushing by
separating the eyelids with fingers. Call a physician.
AFTER INGESTION
If swallowed, wash out mouth with water provided person is
conscious. Call a physician.
5 - Fire Fighting Measures
EXTINGUISHING MEDIA
Suitable: Water spray. Carbon dioxide, dry chemical powder, or
appropriate foam.
SPECIAL RISKS
Specific Hazard(s): Emits toxic fumes under fire conditions.
SPECIAL PROTECTIVE EQUIPMENT FOR FIREFIGHTERS
Wear self-contained breathing apparatus and protective clothing
to prevent contact with skin and eyes.
6 - Accidental Release Measures
PERSONAL PRECAUTION PROCEDURES TO BE FOLLOWED IN CASE OF LEAK OR SPILL
Evacuate area.
PROCEDURE(S) OF PERSONAL PRECAUTION(S)
Wear self-contained breathing apparatus, rubber boots, and heavy
rubber gloves. Wear disposable coveralls and discard them after
use.
METHODS FOR CLEANING UP
Sweep up, place in a bag and hold for waste disposal. Avoid
raising dust. Ventilate area and wash spill site after material
pickup is complete.
7 - Handling and Storage
HANDLING
Directions for Safe Handling: Do not breathe dust. Do not get in
eyes, on skin, on clothing. Avoid prolonged or repeated exposure.
STORAGE
Conditions of Storage: Keep tightly closed.
SPECIAL REQUIREMENTS: Light sensitive.
8 - Exposure Controls / Personal Protection
ENGINEERING CONTROLS
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Use only in a chemical fume hood. Safety shower and eye bath.
GENERAL HYGIENE MEASURES
Wash contaminated clothing before reuse. Wash thoroughly after
handling.
PERSONAL PROTECTIVE EQUIPMENT
Respiratory Protection: Government approved respirator in
nonventilated areas and/or for exposure above the TLV or PEL.
Hand Protection: Compatible chemical-resistant gloves.
Eye Protection: Chemical safety goggles.
9 - Physical and Chemical Properties
Appearance Physical State: Solid
Color: Faintly yellow
Form: Crystalline
Property Value At Temperature or Pressure
pH N/A
BP/BP Range N/A
MP/MP Range 149 掳C
Flash Point N/A
Flammability N/A
Autoignition Temp N/A
Oxidizing Properties N/A
Explosive Properties N/A
Explosion Limits N/A
Vapor Pressure N/A
SG/Density N/A
Partition Coefficient N/A
Viscosity N/A
Vapor Density N/A
Saturated Vapor Conc. N/A
Evaporation Rate N/A
Bulk Density N/A
Decomposition Temp. N/A
Solvent Content N/A
Water Content N/A
Surface Tension N/A
Conductivity N/A
Miscellaneous Data N/A
Solubility Solubility in Water:Soluble.
Solvent: Chloroform Ether Propylene glycol
Acetamide Methanol Ethano
10 - Stability and Reactivity
STABILITY
Stable: Stable.
Conditions to Avoid: Light.
Materials to Avoid: Acids, Acid chlorides, Acid anhydrides,
Oxidizing agents.
HAZARDOUS DECOMPOSITION PRODUCTS
Hazardous Decomposition Products: Carbon monoxide, Carbon dioxide,
Nitrogen oxides, Hydrogen chloride gas.
HAZARDOUS POLYMERIZATION
Hazardous Polymerization: Will not occur
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11 - Toxicological Information
RTECS NUMBER: AB6825000
ACUTE TOXICITY
LDLO
Oral
Woman
400 mg/kg
Remarks: Behavioral:Coma. Vascular:Shock. Lungs, Thorax, or
Respiration:Cyanosis.
LDLO
Intravenous
Infant
30 MG/KG
3D-I
Remarks: Cardiac:Cardiac output. Vascular:BP lowering not
charactertized in autonomic section.
LD50
Oral
Rat
2500 mg/kg
LD50
Intraperitoneal
Rat
1811 MG/KG
LD50
Subcutaneous
Rat
5 GM/KG
Remarks: Gastrointestinal:Hypermotility, diarrhea.
LD50
Intravenous
Rat
171 MG/KG
LD50
Oral
Mouse
1500 mg/kg
LD50
Intraperitoneal
Mouse
1100 MG/KG
LD50
Subcutaneous
Mouse
400 MG/KG
LD50
Intravenous
Mouse
110 MG/KG
Remarks: Behavioral:Somnolence (general depressed activity).
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Behavioral:Ataxia. Lungs, Thorax, or Respiration:Other changes.
LD50
Intravenous
Rabbit
117 MG/KG
LD50
Oral
Guinea pig
500 mg/kg
LD50
Intravenous
Guinea pig
560 MG/KG
SENSITIZATION
Sensitization: Prolonged or repeated exposure may cause allergic
reactions in certain sensitive individuals.
SIGNS AND SYMPTOMS OF EXPOSURE
Exposure may cause depression of the bone marrow and blood
dyscrasias. Nausea, headache, and vomiting.
ROUTE OF EXPOSURE
Skin Contact: May cause skin irritation.
Skin Absorption: May be harmful if absorbed through the skin.
Eye Contact: May cause eye irritation.
Inhalation: Material may be irritating to mucous membranes and
upper respiratory tract. May be harmful if inhaled.
Ingestion: May be harmful if swallowed.
TARGET ORGAN INFORMATION
Blood. Bone marrow. Nerves. Liver.
CHRONIC EXPOSURE - CARCINOGEN
Result: This product is or contains a component that has been
reported to be probably carcinogenic based on its IARC, OSHA,
ACGIH, NTP, or EPA classification.
Woman
Route of Application: Oral
Exposure Time: 60W
Result: Tumorigenic:Carcinogenic by RTECS criteria.
Blood:Changes in bone marrow not included above. Blood:Leukemia
Mouse
Route of Application: Intraperitoneal
Exposure Time: 5W
Result: Tumorigenic:Equivocal tumorigenic agent by RTECS
criteria. Blood:Lymphomas including Hodgkin's disease.
Woman
Route of Application: Oral
Exposure Time: 6W
Result: Tumorigenic:Carcinogenic by RTECS criteria.
Blood:Aplastic anemia. Blood:Leukemia
Man
Route of Application: Oral
Exposure Time: W
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Result: Tumorigenic:Carcinogenic by RTECS criteria.
Blood:Aplastic anemia. Blood:Leukemia
IARC CARCINOGEN LIST
Rating: Group 2A
CHRONIC EXPOSURE - MUTAGEN
Result: Laboratory experiments have shown mutagenic effects.
Human
1 MMOL/L
Cell Type: liver
Unscheduled DNA synthesis
Human
1500 UMO/L
Cell Type: Bone marrow
DNA inhibition
Human
1 MMOL/L
Cell Type: lymphocyte
DNA inhibition
Human
100 MG/L
Cell Type: leukocyte
Cytogenetic analysis
Human
500 MG/L
Cell Type: lymphocyte
Cytogenetic analysis
Rat
1600 UMOL/L
Cell Type: liver
DNA repair
Rat
4 MMOL/L
Cell Type: lung
DNA damage
Rat
2 MMOL/L
Cell Type: liver
DNA damage
Rat
2 MMOL/L
Cell Type: liver
Unscheduled DNA synthesis
Rat
1 GM/KG
Cell Type: HeLa cell
Body fluid assay
Mouse
500 MG/KG
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Intraperitoneal
Cytogenetic analysis
Mouse
50 MG/KG
Parenteral
Cytogenetic analysis
Hamster
30 MG/L
Cell Type: Embryo
Sister chromatid exchange
Domestic Animals
500 UG/L
Cell Type: leukocyte
Cytogenetic analysis
Pig
50 UG/L
Cell Type: leukocyte
Cytogenetic analysis
Cattle, Horse
5 MG/L
Cell Type: fibroblast
Sister chromatid exchange
Cattle, Horse
5 MG/L
Cell Type: lymphocyte
Sister chromatid exchange
CHRONIC EXPOSURE - TERATOGEN
Result: Possible risk of congenital malformation in the fetus.
Species: Rat
Dose: 23 GM/KG
Route of Application: Oral
Exposure Time: (1-21D PREG)
Result: Effects on Embryo or Fetus: Fetotoxicity (except death,
e.g., stunted fetus). Effects on Embryo or Fetus: Other effects
to embryo. Specific Developmental Abnormalities: Homeostasis
Species: Rat
Dose: 2500 MG/KG
Route of Application: Oral
Exposure Time: (9D PREG)
Result: Specific Developmental Abnormalities: Central nervous
system.
Species: Rat
Dose: 2500 MG/KG
Route of Application: Oral
Exposure Time: (11D PREG)
Result: Effects on Embryo or Fetus: Fetal death.
Species: Rat
Dose: 2 GM/KG
Route of Application: Oral
Exposure Time: (8D PREG)
Result: Effects on Embryo or Fetus: Fetotoxicity (except death,
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e.g., stunted fetus). Effects on Embryo or Fetus: Fetal death.
Specific Developmental Abnormalities: Body wall.
Species: Rat
Dose: 3500 MG/KG
Route of Application: Subcutaneous
Exposure Time: (6 -10D PREG)
Result: Specific Developmental Abnormalities: Eye, ear. Specific
Developmental Abnormalities: Urogenital system.
Species: Rat
Dose: 2 GM/KG
Route of Application: Intravenous
Exposure Time: (10-14D PREG)
Result: Effects on Embryo or Fetus: Cytological changes
(including somatic cell genetic material). Effects on Embryo or
Fetus: Other effects to embryo.
Species: Mouse
Dose: 5500 MG/KG
Route of Application: Oral
Exposure Time: (5-15D PREG)
Result: Effects on Embryo or Fetus: Fetotoxicity (except death,
e.g., stunted fetus).
Species: Mouse
Dose: 6 GM/KG
Route of Application: Oral
Exposure Time: (8-10D PREG)
Result: Effects on Embryo or Fetus: Fetal death.
Species: Mouse
Dose: 2 GM/KG
Route of Application: Parenteral
Exposure Time: (12-14D PREG)
Result: Specific Developmental Abnormalities: Central nervous
system.
Species: Rabbit
Dose: 4 GM/KG
Route of Application: Oral
Exposure Time: (6-9D PREG)
Result: Specific Developmental Abnormalities: Musculoskeletal
system.
CHRONIC EXPOSURE - REPRODUCTIVE HAZARD
Species: Rat
Dose: 250 MG/KG
Route of Application: Intraperitoneal
Exposure Time: (3D PREG)
Result: Effects on Fertility: Other measures of fertility
Species: Rat
Dose: 2400 MG/KG
Route of Application: Subcutaneous
Exposure Time: (12-14D PREG)
Result: Effects on Fertility: Post-implantation mortality (e.g.,
dead and/or resorbed implants per total number of implants).
Effects on Embryo or Fetus: Fetotoxicity (except death, e.g.,
stunted fetus).
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Species: Rat
Dose: 3500 MG/KG
Route of Application: Subcutaneous
Exposure Time: (6-10D PREG)
Result: Effects on Fertility: Litter size (e.g.; # fetuses per
litter; measured before birth). Effects on Embryo or Fetus:
Fetotoxicity (except death, e.g., stunted fetus). Effects on
Embryo or Fetus: Fetal death.
Species: Mouse
Dose: 7 GM/KG
Route of Application: Oral
Exposure Time: (6-12D PREG)
Result: Effects on Fertility: Litter size (e.g.; # fetuses per
litter; measured before birth). Specific Developmental
Abnormalities: Musculoskeletal system.
Species: Mouse
Dose: 175 MG/KG
Route of Application: Oral
Exposure Time: (15-21D PREG)
Result: Effects on Newborn: Behavioral.
Species: Rabbit
Dose: 4 GM/KG
Route of Application: Oral
Exposure Time: (8-11D PREG)
Result: Effects on Fertility: Litter size (e.g.; # fetuses per
litter; measured before birth). Effects on Embryo or Fetus:
Fetotoxicity (except death, e.g., stunted fetus). Effects on
Embryo or Fetus: Fetal death.
Species: Rabbit
Dose: 2700 MG/KG
Route of Application: Parenteral
Exposure Time: (11-19D PREG)
Result: Effects on Fertility: Abortion. Effects on Embryo or
Fetus: Fetotoxicity (except death, e.g., stunted fetus). Effects
on Embryo or Fetus: Fetal death.
Species: Rabbit
Dose: 2700 MG/KG
Route of Application: Parenteral
Exposure Time: (2-10D PREG)
Result: Effects on Fertility: Female fertility index (e.g., #
females pregnant per # sperm positive females; # females
pregnant per # females mated ).
12 - Ecological Information
ECOTOXICOLOGICAL EFFECTS
Test Type: EC50 Daphnia
Species: Daphnia magna
Time: 48 h
Value: 345 mg/l
13 - Disposal Considerations
SUBSTANCE DISPOSAL
Contact a licensed professional waste disposal service to dispose
of this material. Observe all federal, state, and local
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environmental regulations.
14 - Transport Information
RID/ADR
Non-hazardous for road transport.
IMDG
Non-hazardous for sea transport.
IATA
Non-hazardous for air transport.
15 - Regulatory Information
CLASSIFICATION AND LABELING ACCORDING TO EU DIRECTIVES
INDICATION OF DANGER: T
Toxic.
R-PHRASES: 45
May cause cancer.
S-PHRASES: 53 45
Avoid exposure - obtain special instructions before use. In
case of accident or if you feel unwell, seek medical advice
immediately (show the label where possible).
16 - Other Information
WARRANTY
The above information is believed to be correct but does not
purport to be all inclusive and shall be used only as a guide. The
information in this document is based on the present state of our
knowledge and is applicable to the product with regard to
appropriate safety precautions. It does not represent any
guarantee of the properties of the product. Sigma-Aldrich Inc.,
shall not be held liable for any damage resulting from handling or
from contact with the above product. See reverse side of invoice
or packing slip for additional terms and conditions of sale.
Copyright 2005 Sigma-Aldrich Co. License granted to make unlimited
paper copies for internal use only.
DISCLAIMER
For R&D use only. Not for drug, household or other uses.
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