SIGMA-ALDRICH
Material Safety Data Sheet
Date Printed: 24/OCT/2004
Date Updated: 12/MAR/2004
Version 1.2
According to 91/155/EEC
Classified as Hazardous according to the criteria of EU Annex 1 and
NOHSC.
1 - Product and Company Information
Product Name ACTINOMYCIN D FROM STREPTOMYCES SP.
Product Number 01817
Company Sigma-Aldrich Pty, Ltd
Unit 2, 14 Anella Avenue
Castle Hill NSW 1765
Australia
Technical Phone # +61 2 9841 0555
Fax +61 2 9841 0500
Emergency Phone # +61 2 9841 0566
2 - Composition/Information on Ingredients
Product Name CAS # EC no Annex I
Index Number
(-)-ACTINOMYCIN D 50-76-0 200-063-6 None
Formula C62H86N12O16
Molecular Weight 1,255.5 AMU
Synonyms Actinomycin I(sub 1) * Actinomycin I *
Actinomycin IV * Actinomycin C1 * Actinomycin 11
cosmegen * Actinomycindioic D acid, dilactone *
Actinomycin X 1 * Actinomycin 7 * Chounghwamycin
B * Cosmegen * Actinomycin A IV * Dactinomycin *
Dactinomycin D * Dilactone actinomycin D acid *
Dilactone actinomycindioic D acid * Lyovac
cosmegen * Meractinomycin * NCI-C04682 * NSC
3053 * Oncostatin K * X 97
3 - Hazards Identification
SPECIAL INDICATION OF HAZARDS TO HUMANS AND THE ENVIRONMENT
Very toxic if swallowed. Limited evidence of a carcinogenic
effect. May cause sensitization by inhalation and skin contact.
4 - First Aid Measures
AFTER INHALATION
If inhaled, remove to fresh air. If not breathing give
artificial respiration. If breathing is difficult, give oxygen.
AFTER SKIN CONTACT
In case of skin contact, flush with copious amounts of water for
at least 15 minutes. Remove contaminated clothing and shoes.
Call a physician.
AFTER EYE CONTACT
In case of contact with eyes, flush with copious amounts of
water for at least 15 minutes. Assure adequate flushing by
separating the eyelids with fingers. Call a physician.
AFTER INGESTION
If swallowed, wash out mouth with water provided person is
conscious. Call a physician immediately.
5 - Fire Fighting Measures
EXTINGUISHING MEDIA
Suitable: Water spray. Carbon dioxide, dry chemical powder, or
appropriate foam.
SPECIAL RISKS
Specific Hazard(s): Emits toxic fumes under fire conditions.
SPECIAL PROTECTIVE EQUIPMENT FOR FIREFIGHTERS
Wear self-contained breathing apparatus and protective clothing
to prevent contact with skin and eyes.
6 - Accidental Release Measures
PERSONAL PRECAUTION PROCEDURES TO BE FOLLOWED IN CASE OF LEAK OR SPILL
Evacuate area.
PROCEDURE(S) OF PERSONAL PRECAUTION(S)
Wear self-contained breathing apparatus, rubber boots, and heavy
rubber gloves.
METHODS FOR CLEANING UP
Sweep up, place in a bag and hold for waste disposal. Avoid
raising dust. Ventilate area and wash spill site after material
pickup is complete.
7 - Handling and Storage
HANDLING
Directions for Safe Handling: Do not breathe dust. Do not get in
eyes, on skin, on clothing. Avoid prolonged or repeated exposure.
STORAGE
Conditions of Storage: Keep tightly closed.
Store at 2-8掳C
8 - Exposure Controls / Personal Protection
ENGINEERING CONTROLS
Safety shower and eye bath. Use only in a chemical fume hood.
GENERAL HYGIENE MEASURES
Wash contaminated clothing before reuse. Wash thoroughly after
handling.
PERSONAL PROTECTIVE EQUIPMENT
Respiratory Protection: Government approved respirator.
Hand Protection: Compatible chemical-resistant gloves.
Eye Protection: Chemical safety goggles.
9 - Physical and Chemical Properties
Appearance Physical State: Solid
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Color: Deep red
Form: Powder
Property Value At Temperature or Pressure
pH N/A
BP/BP Range N/A
MP/MP Range 251 掳C
Flash Point N/A
Flammability N/A
Autoignition Temp N/A
Oxidizing Properties N/A
Explosive Properties N/A
Explosion Limits N/A
Vapor Pressure N/A
SG/Density N/A
Partition Coefficient N/A
Viscosity N/A
Vapor Density N/A
Saturated Vapor Conc. N/A
Evaporation Rate N/A
Bulk Density N/A
Decomposition Temp. N/A
Solvent Content N/A
Water Content N/A
Surface Tension N/A
Conductivity N/A
Miscellaneous Data N/A
Solubility N/A
10 - Stability and Reactivity
STABILITY
Stable: Stable.
Materials to Avoid: Strong oxidizing agents, Strong acids, Strong
bases.
HAZARDOUS DECOMPOSITION PRODUCTS
Hazardous Decomposition Products: Carbon monoxide, Carbon dioxide,
Nitrogen oxides.
HAZARDOUS POLYMERIZATION
Hazardous Polymerization: Will not occur
11 - Toxicological Information
RTECS NUMBER: AU1575000
ACUTE TOXICITY
LD50
Oral
Rat
7.2 mg/kg
Remarks: Gastrointestinal:Hypermotility, diarrhea. Blood:Other
changes.
LD50
Intraperitoneal
Rat
100 UG/KG
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LD50
Subcutaneous
Rat
800 UG/KG
Remarks: Gastrointestinal:Hypermotility, diarrhea. Blood:Other
changes.
LD50
Intravenous
Rat
460 UG/KG
Remarks: Gastrointestinal:Hypermotility, diarrhea. Blood:Other
changes.
LD50
Oral
Mouse
13 mg/kg
Remarks: Gastrointestinal:Hypermotility, diarrhea. Blood:Other
changes.
LD50
Intraperitoneal
Mouse
750 UG/KG
Remarks: Blood:Changes in bone marrow not included above. Skin
and Appendages: Decreased immune response.
LD50
Subcutaneous
Mouse
500 UG/KG
LD50
Intravenous
Mouse
1025 UG/KG
LD50
Oral
Bird (wild)
1 mg/kg
IRRITATION DATA
Skin
Rabbit
5 mg
24H
Remarks: Rinsed
SENSITIZATION
Sensitization: May cause allergic reaction.
SIGNS AND SYMPTOMS OF EXPOSURE
Inhalation may result in spasm, inflammation and edema of the
larynxand bronchi, chemical pneumonitis, and pulmonary edema.
Exposure can cause: Anemia. Drowsiness. Weakness.
ROUTE OF EXPOSURE
Skin Contact: May cause skin irritation.
Skin Absorption: May be harmful if absorbed through the skin.
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Eye Contact: May cause eye irritation.
Inhalation: May be harmful if inhaled. Material may be
irritating to mucous membranes and upper respiratory tract.
Ingestion: May be fatal if swallowed.
TARGET ORGAN INFORMATION
Bone marrow. Liver. Kidneys.
CHRONIC EXPOSURE - CARCINOGEN
Result: This product is or contains a component that has been
reported to be possibly carcinogenic based on its IARC, ACGIH,
NTP, or EPA classification.
Rat
Route of Application: Intraperitoneal
Exposure Time: 17W
Result: Tumorigenic:Carcinogenic by RTECS criteria.
Tumorigenic:Tumors at site or application.
Mouse
Route of Application: Subcutaneous
Exposure Time: 18W
Result: Tumorigenic:Equivocal tumorigenic agent by RTECS
criteria. Tumorigenic:Tumors at site or application.
Rat
Route of Application: Intraperitoneal
Exposure Time: 26W
Result: Tumorigenic:Neoplastic by RTECS criteria.
Tumorigenic:Tumors at site or application.
IARC CARCINOGEN LIST
Rating: Group 3
CHRONIC EXPOSURE - MUTAGEN
Human
400 UG/L
15M
Cell Type: HeLa cell
DNA damage
Human
10 UG/L
Cell Type: Other cell types
DNA damage
Human
40 UG/L
Cell Type: Other cell types
Other mutation test systems
Human
300 PMOL/L
Cell Type: Other cell types
DNA inhibition
Human
10 UG/L
2H
Cell Type: Other cell types
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DNA inhibition
Human
700 NMOL/L
Cell Type: HeLa cell
DNA inhibition
Human
40 MG/L
Cell Type: Other cell types
DNA inhibition
Human
250 UG/L
Cell Type: HeLa cell
Other mutation test systems
Human
200 UG/L
2H
Cell Type: lymphocyte
Cytogenetic analysis
Human
200 UG/L
24H
Cell Type: leukocyte
Cytogenetic analysis
Human
250 UG/L
1H
Cell Type: HeLa cell
Cytogenetic analysis
Human
1 NMOL/L
Cell Type: lymphocyte
Sister chromatid exchange
Human
1 MG/L
Cell Type: fibroblast
Mutation in mammalian somatic cells.
Rat
500 NMOL/L
Cell Type: Other cell types
DNA damage
Rat
100 UG/KG
Intraperitoneal
DNA
Rat
100 MG/L
Cell Type: liver
DNA damage
Rat
16 UMOL/L
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Cell Type: lymphocyte
DNA
Rat
20 UG/KG
Intravenous
Other mutation test systems
Rat
50 NMOL/L
Cell Type: liver
Unscheduled DNA synthesis
Rat
2 MG/KG
Intraperitoneal
Other mutation test systems
Rat
150 MG/L
Cell Type: Other cell types
Other mutation test systems
Chicken
5 UG/L
Cell Type: Embryo
Other mutation test systems
Rat
50 UG/KG
Parenteral
Other mutation test systems
Rat
1 MG/L
Cell Type: Other cell types
DNA inhibition
Rat
1 MG/L
Cell Type: Other cell types
Other mutation test systems
Rat
250 MG/KG
Intravenous
Cytogenetic analysis
Mouse
1 MG/KG
Intraperitoneal
Micronucleus test
Mouse
6 UG/L
Cell Type: lymphocyte
Micronucleus test
Mouse
500 NMOL/L
Cell Type: liver
Unscheduled DNA synthesis
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Mouse
100 UG/L
Cell Type: Embryo
Morphological transformation.
Mouse
5 UMOL/L
Cell Type: lymphocyte
DNA damage
Mouse
40 UMOL/L
Cell Type: Ascites tumor
DNA damage
Mouse
800 UG/KG
Cell Type: Ascites tumor
DNA damage
Mouse
20 MG/L
Cell Type: mammary gland
DNA damage
Mouse
1500 UG/L
Cell Type: leukocyte
DNA damage
Mouse
2 MG/L
Cell Type: Other cell types
Other mutation test systems
Mouse
50 MG/L
Cell Type: Other cell types
DNA inhibition
Mouse
800 MG/L
Cell Type: leukocyte
DNA inhibition
Mouse
750 UG/KG
Intravenous
DNA inhibition
Mouse
20 GM/KG
Intraperitoneal
DNA inhibition
Mouse
40 UG/L
Cell Type: lymphocyte
DNA inhibition
Mouse
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10 UG/L
Cell Type: Embryo
DNA inhibition
Mouse
40 UG/L
Cell Type: lymphocyte
Other mutation test systems
Mouse
800 MG/L
Cell Type: leukocyte
Other mutation test systems
Mouse
1 UMOL/L
Cell Type: Ascites tumor
Other mutation test systems
Mouse
200 UG/L
Cell Type: fibroblast
Other mutation test systems
Mouse
4 MG/KG
Intraperitoneal
Other mutation test systems
Mouse
1330 UG/KG
Intraperitoneal
Other mutation test systems
Mouse
5 UG/L
Cell Type: Other cell types
Other mutation test systems
Mouse
1 MG/KG
Parenteral
Cytogenetic analysis
Mouse
100 UG/L
Cell Type: fibroblast
Cytogenetic analysis
Mouse
6 UG/L
Cell Type: lymphocyte
Cytogenetic analysis
Mouse
200 UG/L
4H
Cell Type: Other cell types
Cytogenetic analysis
Mouse
500 UG/KG
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Intraperitoneal
Sister chromatid exchange
Mouse
4 UG/L
Cell Type: lymphocyte
Mutation in mammalian somatic cells.
Mouse
1 MG/KG
Intraperitoneal
5D
sperm
Mouse
1 MG/KG
Intravenous
sperm
Hamster
2500 UG/L
Cell Type: lung
DNA
Hamster
5 MG/L
Cell Type: ovary
DNA damage
Hamster
80 UG/L
Cell Type: Other cell types
DNA inhibition
Hamster
5 MG/L
Cell Type: ovary
DNA inhibition
Hamster
250 UG/L
Cell Type: ovary
Other mutation test systems
Hamster
2400 UG/L
Cell Type: Other cell types
Other mutation test systems
Hamster
20 UG/L
Cell Type: lung
Cytogenetic analysis
Hamster
16 UG/KG
Parenteral
Cytogenetic analysis
Hamster
50 UG/L
Cell Type: Embryo
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Cytogenetic analysis
Hamster
100 UG/L
Cell Type: ovary
Cytogenetic analysis
Hamster
1 MG/L
Cell Type: fibroblast
Cytogenetic analysis
Hamster
100 UG/L
Cell Type: Other cell types
Cytogenetic analysis
Hamster
10 UG/L
Cell Type: fibroblast
Sister chromatid exchange
Hamster
10 UG/L
Cell Type: ovary
Sister chromatid exchange
Hamster
10 UG/L
Cell Type: lung
Mutation in mammalian somatic cells.
Hamster
40 UG/L
Cell Type: ovary
Mutation in mammalian somatic cells.
Hamster
16 UG/KG
Parenteral
sperm
Monkey
5 MG/L
Cell Type: kidney
DNA damage
Bird
1 MMOL/L
Cell Type: leukocyte
DNA damage
Bird
1 MMOL/L
Cell Type: liver
DNA damage
Bird
1 MMOL/L
Cell Type: kidney
DNA damage
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Bird
1 MMOL/L
Cell Type: Other cell types
DNA damage
Monkey
10 UG/L
Cell Type: kidney
DNA inhibition
Chicken
25 UG/L
Cell Type: fibroblast
Other mutation test systems
Chicken
25 UG/L
Cell Type: fibroblast
DNA inhibition
Chicken
25 UG/L
Cell Type: fibroblast
Other mutation test systems
Chicken
28 MG/L
Cell Type: Embryo
Cytogenetic analysis
Chicken
25 UG/L
Cell Type: fibroblast
Cytogenetic analysis
Mammal
8 UMOL/L
Cell Type: lymphocyte
DNA damage
Mammal
1 MG/L
Cell Type: sperm
DNA damage
Rabbit
330 UG/L
Cell Type: kidney
Other mutation test systems
Chicken
100 UG/L
Cell Type: Embryo
DNA inhibition
Rabbit
20 MG/L
Cell Type: Bone marrow
Other mutation test systems
Mammal
1 MG/L
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Cell Type: Other cell types
Cytogenetic analysis
CHRONIC EXPOSURE - TERATOGEN
Species: Rat
Dose: 70 NG/KG
Route of Application: Intraperitoneal
Exposure Time: (8D PREG)
Result: Specific Developmental Abnormalities: Musculoskeletal
system. Specific Developmental Abnormalities: Other
developmental abnormalities.
Species: Rat
Dose: 150 UG/KG
Route of Application: Intraperitoneal
Exposure Time: (8D PREG)
Result: Specific Developmental Abnormalities: Central nervous
system. Specific Developmental Abnormalities: Eye, ear. Specific
Developmental Abnormalities: Craniofacial (including nose and
tongue).
Species: Rat
Dose: 200 UG/KG
Route of Application: Subcutaneous
Exposure Time: (12D PREG)
Result: Specific Developmental Abnormalities: Musculoskeletal
system. Effects on Embryo or Fetus: Fetal death.
Species: Mouse
Dose: 150 UG/KG
Route of Application: Intraperitoneal
Exposure Time: (8D PREG)
Result: Specific Developmental Abnormalities: Eye, ear. Specific
Developmental Abnormalities: Craniofacial (including nose and
tongue).
Species: Mouse
Dose: 100 UG/KG
Route of Application: Intraperitoneal
Exposure Time: (6D PREG)
Result: Specific Developmental Abnormalities: Musculoskeletal
system.
Species: Mouse
Dose: 214 UG/KG
Route of Application: Intraperitoneal
Exposure Time: (5-7D PREG)
Result: Specific Developmental Abnormalities: Body wall.
Species: Rabbit
Dose: 13 MG/KG
Route of Application: Parenteral
Exposure Time: (1D PREG)
Result: Effects on Embryo or Fetus: Fetotoxicity (except death,
e.g., stunted fetus). Effects on Embryo or Fetus: Fetal death.
Effects on Embryo or Fetus: Other effects to embryo.
Species: Hamster
Dose: 100 UG/KG
Route of Application: Intraperitoneal
Exposure Time: (8D PREG)
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Result: Specific Developmental Abnormalities: Central nervous
system. Specific Developmental Abnormalities: Musculoskeletal
system.
CHRONIC EXPOSURE - REPRODUCTIVE HAZARD
Species: Rat
Dose: 250 UG/KG
Route of Application: Oral
Exposure Time: (13D PREG)
Result: Effects on Fertility: Post-implantation mortality (e.g.,
dead and/or resorbed implants per total number of implants).
Species: Rat
Dose: 70 NG/KG
Route of Application: Intraperitoneal
Exposure Time: (12D PREG)
Result: Effects on Fertility: Litter size (e.g.; # fetuses per
litter; measured before birth). Effects on Embryo or Fetus:
Fetotoxicity (except death, e.g., stunted fetus). Effects on
Embryo or Fetus: Other effects to embryo.
Species: Rat
Dose: 100 UG/KG
Route of Application: Intraperitoneal
Exposure Time: (18-19D PREG)
Result: Effects on Newborn: Behavioral.
Species: Rat
Dose: 150 UG/KG
Route of Application: Intraperitoneal
Exposure Time: (8D PREG)
Result: Effects on Fertility: Post-implantation mortality (e.g.,
dead and/or resorbed implants per total number of implants).
Effects on Embryo or Fetus: Fetotoxicity (except death, e.g.,
stunted fetus). Effects on Embryo or Fetus: Fetal death.
Species: Rat
Dose: 200 UG/KG
Route of Application: Unreported
Exposure Time: (10D PREG)
Result: Effects on Fertility: Litter size (e.g.; # fetuses per
litter; measured before birth).
Species: Mouse
Dose: 20 MG/KG
Route of Application: Oral
Exposure Time: (8-12D PREG)
Result: Effects on Newborn: Live birth index (# fetuses per
litter; measured after birth). Effects on Newborn: Viability
index (e.g., # alive at day 4 per # born alive).
Species: Mouse
Dose: 200 UG/KG
Route of Application: Intraperitoneal
Exposure Time: (2D PREG)
Result: Effects on Fertility: Other measures of fertility
Species: Mouse
Dose: 100 UG/KG
Route of Application: Intraperitoneal
Exposure Time: (8D PREG)
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Result: Effects on Fertility: Post-implantation mortality (e.g.,
dead and/or resorbed implants per total number of implants).
Effects on Embryo or Fetus: Fetotoxicity (except death, e.g.,
stunted fetus). Effects on Embryo or Fetus: Fetal death.
Species: Mouse
Dose: 300 UG/KG
Route of Application: Intravenous
Exposure Time: (1D MALE)
Result: Paternal Effects: Spermatogenesis (including genetic
material, sperm morphology,motility, and count).
Species: Rabbit
Dose: 5 UG/KG
Route of Application: Parenteral
Exposure Time: (1D PREG)
Result: Effects on Fertility: Other measures of fertility
Species: Rabbit
Dose: 50 UG/KG
Route of Application: Intrauterine
Exposure Time: (1D PREG)
Result: Maternal Effects: Other effects.
Species: Guinea pig
Dose: 40 UG/KG
Route of Application: Intrauterine
Exposure Time: (1D PRE)
Result: Maternal Effects: Menstrual cycle changes or disorders.
Species: Hamster
Dose: 100 UG/KG
Route of Application: Intraperitoneal
Exposure Time: (8D PREG)
Result: Effects on Fertility: Post-implantation mortality (e.g.,
dead and/or resorbed implants per total number of implants).
Specific Developmental Abnormalities: Body wall. Specific
Developmental Abnormalities: Craniofacial (including nose and
tongue).
Species: Hamster
Dose: 200 UG/KG
Route of Application: Intraperitoneal
Exposure Time: (7-8D PREG)
Result: Effects on Fertility: Other measures of fertility
Species: Hamster
Dose: 16 UG/KG
Route of Application: Intratesticular
Exposure Time: (1D MALE)
Result: Paternal Effects: Spermatogenesis (including genetic
material, sperm morphology,motility, and count).
12 - Ecological Information
No data available.
13 - Disposal Considerations
SUBSTANCE DISPOSAL
Contact a licensed professional waste disposal service to dispose
of this material. Dissolve or mix the material with a combustible
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solvent and burn in a chemical incinerator equipped with an
afterburner and scrubber. Observe all federal, state, and local
environmental regulations.
14 - Transport Information
RID/ADR
UN#: 2811
Class: 6.1
PG: II
Proper Shipping Name: Toxic solid, organic, n.o.s.
IMDG
UN#: 2811
Class: 6.1
PG: II
Proper Shipping Name: Toxic solid, organic, n.o.s.
Marine Pollutant: No
Severe Marine Pollutant: No
Technical Name: Required
IATA
UN#: 2811
Class: 6.1
PG: II
Proper Shipping Name: Toxic solid, organic, n.o.s.
Inhalation Packing Group I: No
Technical Name: Required
15 - Regulatory Information
CLASSIFICATION AND LABELING ACCORDING TO EU DIRECTIVES
INDICATION OF DANGER: T+
Very toxic.
R-PHRASES: 28 40 42/43
Very toxic if swallowed. Limited evidence of a carcinogenic
effect. May cause sensitization by inhalation and skin contact.
S-PHRASES: 22 28 36/37 45
Do not breathe dust. After contact with skin, wash immediately
with plenty of soap-suds. Wear suitable protective clothing and
gloves. In case of accident or if you feel unwell, seek medical
advice immediately (show the label where possible).
COUNTRY SPECIFIC INFORMATION
Germany
WGK: 3
16 - Other Information
WARRANTY
The above information is believed to be correct but does not
purport to be all inclusive and shall be used only as a guide. The
information in this document is based on the present state of our
knowledge and is applicable to the product with regard to
appropriate safety precautions. It does not represent any
guarantee of the properties of the product. Sigma-Aldrich Inc.,
shall not be held liable for any damage resulting from handling or
from contact with the above product. See reverse side of invoice
or packing slip for additional terms and conditions of sale.
Copyright 2004 Sigma-Aldrich Co. License granted to make unlimited
paper copies for internal use only.
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DISCLAIMER
For R&D use only. Not for drug, household or other uses.
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