SIGMA-ALDRICH
Material Safety Data Sheet
Date Printed: 05/SEP/2005
Date Updated: 14/MAR/2004
Version 1.2
According to 91/155/EEC
1 - Product and Company Information
Product Name ACRIDINE ORANGE BASE
Product Number 235474
Company Sigma-Aldrich Pty. Ltd.
12 Anella Avenue
Castle Hill NSW 2154
Australia
Technical Phone # +61 2 9841 0555 (1800 800 097)
Fax +61 2 9841 0500 (1800 800 096)
Emergency Phone # +44 8701906777 (1800 448 465)
2 - Composition/Information on Ingredients
Product Name CAS # EC no Annex I
Index Number
ACRIDINE ORANGE BASE 494-38-2 None None
Formula C17H19N3
Molecular Weight 265.36 AMU
Synonyms Acridinediamine, N,N,N',N'-tetramethyl- (9CI) *
Acridine Orange Base * Acridine Orange Free Base
* 2,8-Bisdimethylaminoacridine *
3,6-Bis(dimethylamino)acridine *
3,6-Bis-(dimethylamino)akridin (Czech) *
Brilliant Acridine Orange E * C.I. 46005B * C.I.
No. 46005:1 * C.I. Solvent Orange 15 *
3,6-Di(dimethylamino)acridine * Euchrysine *
Oranz akridinova (Czech) * Oranz rozpoustedlova
15 (Czech) * Rhoduline Orange * Rhoduline Orange
N * N,N,N',N'-Tetramethyl-3,6-acridinediamine *
N,N,N',N'-Tetramethylacridinediamine * Waxoline
Orange A
3 - Hazards Identification
SPECIAL INDICATION OF HAZARDS TO HUMANS AND THE ENVIRONMENT
Not hazardous according to Directive 67/548/EEC.
4 - First Aid Measures
AFTER INHALATION
If inhaled, remove to fresh air. If breathing becomes difficult,
call a physician.
AFTER SKIN CONTACT
In case of contact, immediately wash skin with soap and copious
amounts of water.
AFTER EYE CONTACT
In case of contact with eyes, flush with copious amounts of
water for at least 15 minutes. Assure adequate flushing by
separating the eyelids with fingers. Call a physician.
AFTER INGESTION
If swallowed, wash out mouth with water provided person is
conscious. Call a physician.
5 - Fire Fighting Measures
EXTINGUISHING MEDIA
Suitable: Water spray. Carbon dioxide, dry chemical powder, or
appropriate foam.
SPECIAL RISKS
Specific Hazard(s): Emits toxic fumes under fire conditions.
SPECIAL PROTECTIVE EQUIPMENT FOR FIREFIGHTERS
Wear self-contained breathing apparatus and protective clothing
to prevent contact with skin and eyes.
6 - Accidental Release Measures
PROCEDURE(S) OF PERSONAL PRECAUTION(S)
Exercise appropriate precautions to minimize direct contact with
skin or eyes and prevent inhalation of dust.
METHODS FOR CLEANING UP
Sweep up, place in a bag and hold for waste disposal. Avoid
raising dust. Ventilate area and wash spill site after material
pickup is complete.
7 - Handling and Storage
HANDLING
Directions for Safe Handling: Avoid inhalation. Avoid contact
with eyes, skin, and clothing. Avoid prolonged or repeated
exposure.
STORAGE
Conditions of Storage: Keep tightly closed.
8 - Exposure Controls / Personal Protection
ENGINEERING CONTROLS
Safety shower and eye bath. Mechanical exhaust required.
GENERAL HYGIENE MEASURES
Wash thoroughly after handling.
PERSONAL PROTECTIVE EQUIPMENT
Respiratory Protection: Wear dust mask.
Hand Protection: Protective gloves.
Eye Protection: Chemical safety goggles.
9 - Physical and Chemical Properties
Appearance Physical State: Solid
Property Value At Temperature or Pressure
pH N/A
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BP/BP Range N/A
MP/MP Range 165 掳C
Flash Point N/A
Flammability N/A
Autoignition Temp N/A
Oxidizing Properties N/A
Explosive Properties N/A
Explosion Limits N/A
Vapor Pressure N/A
SG/Density N/A
Partition Coefficient N/A
Viscosity N/A
Vapor Density N/A
Saturated Vapor Conc. N/A
Evaporation Rate N/A
Bulk Density N/A
Decomposition Temp. N/A
Solvent Content N/A
Water Content N/A
Surface Tension N/A
Conductivity N/A
Miscellaneous Data N/A
Solubility N/A
10 - Stability and Reactivity
STABILITY
Stable: Stable.
Materials to Avoid: Strong oxidizing agents.
HAZARDOUS DECOMPOSITION PRODUCTS
Hazardous Decomposition Products: Carbon monoxide, carbon dioxide,
and nitrogen oxides.
HAZARDOUS POLYMERIZATION
Hazardous Polymerization: Will not occur
11 - Toxicological Information
RTECS NUMBER: AR7600000
ACUTE TOXICITY
LD50
Subcutaneous
Mouse
250 MG/KG
SIGNS AND SYMPTOMS OF EXPOSURE
To the best of our knowledge, the chemical, physical, and
toxicological properties have not been thoroughly investigated.
ROUTE OF EXPOSURE
Skin Contact: May cause skin irritation.
Skin Absorption: May be harmful if absorbed through the skin.
Eye Contact: May cause eye irritation.
Inhalation: Material may be irritating to mucous membranes and
upper respiratory tract. May be harmful if inhaled.
Ingestion: May be harmful if swallowed.
TARGET ORGAN INFORMATION
Blood.
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CHRONIC EXPOSURE - CARCINOGEN
Result: This product is or contains a component that is not
classifiable as to its carcinogenicity based on its IARC, ACGIH,
NTP, or EPA classification.
Mouse
Route of Application: Skin
Result: Liver:Tumors. Tumorigenic:Equivocal tumorigenic agent by
RTECS criteria.
Mouse
Route of Application: Subcutaneous
Exposure Time: 63W
Result: Tumorigenic:Tumors at site or application.
Tumorigenic:Equivocal tumorigenic agent by RTECS criteria.
Blood:Lymphomas including Hodgkin's disease.
IARC CARCINOGEN LIST
Rating: Group 3
CHRONIC EXPOSURE - MUTAGEN
Result: Laboratory experiments have shown mutagenic effects.
Human
20 UMOL/L
Cell Type: Bone marrow
DNA
Human
100 MG/L
Cell Type: fibroblast
DNA damage
Human
2 UMOL/L
Cell Type: HeLa cell
DNA inhibition
Human
10 UMOL/L
Cell Type: HeLa cell
Cytogenetic analysis
Human
500 UG/L
Cell Type: lymphocyte
Sister chromatid exchange
Rat
1 MMOL/L
Cell Type: liver
Unscheduled DNA synthesis
Mouse
100 MG/KG
Intraperitoneal
Micronucleus test
Mouse
100 MG/KG
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Intraperitoneal
specific locus test
Mouse
20 UMOL/L
Cell Type: Ascites tumor
DNA damage
Mouse
200 MG/KG
Intraperitoneal
DNA inhibition
Mouse
2500 UG/L
Cell Type: lymphocyte
Mutation in mammalian somatic cells.
Hamster
1 UG/L
Cell Type: Embryo
Morphological transformation.
Hamster
1 MG/L
Cell Type: lung
Cytogenetic analysis
Hamster
20 UMOL/L
5H
Cell Type: ovary
Cytogenetic analysis
Hamster
1 MG/L
Cell Type: lung
Sister chromatid exchange
Hamster
1 MG/L
Cell Type: ovary
Mutation in mammalian somatic cells.
Domestic Animals
1 UMOL/L
Cell Type: kidney
Unscheduled DNA synthesis
Mammal
10 PPH
Cell Type: lymphocyte
DNA
Mammal
1 UMOL/L
Cell Type: lymphocyte
Unscheduled DNA synthesis
Chicken
1695 UG/KG
Parenteral
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Cytogenetic analysis
Chicken
1695 UG/KG
Parenteral
Sister chromatid exchange
12 - Ecological Information
No data available.
13 - Disposal Considerations
SUBSTANCE DISPOSAL
Contact a licensed professional waste disposal service to dispose
of this material. Dissolve or mix the material with a combustible
solvent and burn in a chemical incinerator equipped with an
afterburner and scrubber. Observe all federal, state, and local
environmental regulations.
14 - Transport Information
RID/ADR
Non-hazardous for road transport.
IMDG
Non-hazardous for sea transport.
IATA
Non-hazardous for air transport.
15 - Regulatory Information
CLASSIFICATION AND LABELING ACCORDING TO EU DIRECTIVES
S-PHRASES: 22-24/25
Do not breathe dust. Avoid contact with skin and eyes.
Not hazardous according to Directive 67/548/EEC.
SWITZERLAND
SWISS POISON CLASS: 1*
16 - Other Information
WARRANTY
The above information is believed to be correct but does not
purport to be all inclusive and shall be used only as a guide. The
information in this document is based on the present state of our
knowledge and is applicable to the product with regard to
appropriate safety precautions. It does not represent any
guarantee of the properties of the product. Sigma-Aldrich Inc.,
shall not be held liable for any damage resulting from handling or
from contact with the above product. See reverse side of invoice
or packing slip for additional terms and conditions of sale.
Copyright 2005 Sigma-Aldrich Co. License granted to make unlimited
paper copies for internal use only.
DISCLAIMER
For R&D use only. Not for drug, household or other uses.
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