Material Safety Data Sheet Ba 4-Plex Assay
Effective Date: March 8th, 2010
Supersedes: April 22nd, 2009
1. Identification of the Material and Company/Undertaking
Ba 4-Plex Assay Catalog #: GXBA-100N-010
Product name:
Not applicable
Common name:
Not applicable
Compound name:
Manufacturer: Telephone Numbers:
US: (888) 838-3222 (6 AM ?5 PM Pacific time US)
Cepheid
EU: +33.563.82.53.19
904 Caribbean Drive
Outside of the US: 1 (408) 541-4191
Sunnyvale, CA 94089
USA 24-Hour Emergency Telephone:
CHEMTREC (800) 424-9300
Outside of the US: 1 (703) 741-5500
US E-mail: techsupport@cepheid.com
EU E-mail: techsupport@cepheideurope.fr
The following MSDS is for the final finished product only as used in the laboratory that contains five (5)
components, some of which are in cartridges, and some of which are contained in ampoules. If
manufacturing this product, consult the MSDSs for the individual ingredients and reagents.
2. Hazards Identification
This product contains 5 parts ?3 of the parts are beads, which are freeze-
Appearance:
dried white powders and are contained in cartridges. Two components are
reagents that are liquids that are clear, colorless liquids.
CAUTION for handling of all kit components
Signal Word:
Hazard Overview: Reagent 1 contains sodium hypochlorite which at the very low
concentration in the mixture is an irritant to the eye.
Reagent 2 contains sodium azide, which at the concentration
contained in the solution (<0.05%) is not considered acutely toxic, but
it should be evaluated for its disposal as if allowed to accumulate in
metal piping it has the potential to form explosive mixtures. Sodium
azide also has the potential to be toxic to the aquatic environment;
may cause long 璽erm adverse effects on the aquatic environment.
300-7570 Rev C Page 1
Material Safety Data Sheet Ba 4-Plex Assay
Effective Date: March 8th, 2010
Supersedes: April 22nd, 2009
Bead components as contained in cartridges will not present a hazard
under normal use conditions; if cartridge is broken or damaged and
beads are spilled or released, contact may cause reversible skin and
eye irritation, and if repeatedly contact may cause an allergic skin
reaction.
Statement of Known
Reagent 1 is an eye irritant. Avoid skin contact, eye contact and
Hazard:
inhalation. Wear eye protection and skin protection to avoid contact.
Reagent 2 contains sodium azide, which at the concentration contained in
the product may liberate gas if contacting acids or if allowed to
accumulate in metal piping cause an explosive reaction. It is also toxic to
the aquatic environment and has potential to cause adverse effects on the
aquatic environment. Dispose of according to local, state and federal
regulations.
EU Indicator of Danger: Reagent 1 ?Xi (Irritant)
Reagent 2 ?Xn; N (Harmful to the environment)
All other components of this product: Not applicable
EU Risk Phrases: Reagent 1 - R36 ?Irritating to eyes
Reagent 2- R32 ?Contact with acid liberates very toxic gas
R52/53 ?Harmful to aquatic environment; may
cause long-term adverse effects on the aquatic
environment.
All other components of this product: Not applicable
3. Composition Information on Ingredients
This product consists of a cartridge with three reagent beads to which aqueous reagents are
added as part of an assay.
SPC Bead is a freeze-dried powder bead as contained in the cartridge with the following
hazardous ingredients:
EINECs / % Composition EU Risk Phrase
Ingredient CAS#
ELINCS #
7365-45-9 230-907-9
HEPES Acid and Salt <5 R36/37/38
(For Acid) (For Acid)
300-7570 Rev C Page 2
Material Safety Data Sheet Ba 4-Plex Assay
Effective Date: March 8th, 2010
Supersedes: April 22nd, 2009
All other ingredients of SPC Bead are either non-hazardous under US and EU regulations or GHS
guidelines and/or at concentrations less than 1% in the mixture.
EZR Bead is a freeze-dried powder bead as contained in the cartridge with the following
hazardous ingredients:
EINECs / % Composition EU Risk Phrase
Ingredient CAS#
ELINCS #
7365-45-9 230-907-9
HEPES Acid and Salt <10 R36/37/38
(For Acid) (For Acid)
Magnesium Chloride 7786-30-3 232-094-6 <3 R36/37/38
Bovine Serum Albumin 9048-46-8 232-936-2 <3 R42
Tween 20 9005-64-5 200-315-5 <3 R36/37/38
All other ingredients of the EZR Bead are either non-hazardous under US and EU regulations or GHS
guidelines and/or at concentrations less than 1% in the mixture.
TSR Bead is a freeze-dried powder bead as contained in the cartridge with the following
hazardous ingredients:
EINECs / % Composition EU Risk Phrase
Ingredient CAS#
ELINCS #
7365-45-9 230-907-9
HEPES Acid and Salt <20 R36/37/38
(For Acid) (For Acid)
Bovine Serum Albumin 9048-46-8 232-936-2 <3 R42
Tween 20 9005-64-5 200-315-5 <3 R36/37/38
All other ingredients of TSR Bead are either non-hazardous under US and EU regulations or GHS
guidelines and/or at concentrations less than 1% in the mixture.
Reagent 1 contains the following hazardous ingredients:
EINECs / % Composition EU Risk Phrase
Ingredient CAS#
ELINCS #
Sodium hypochlorite 7681-52-9 231-668-3 <0.1.1 R20 R21 R22 R34 R41
Reagent 1 is a very dilute bleach solution in water. Although the hazardous ingredient is much less than
1%, it may be irritating to the eyes.
The other ingredient in Reagent 1 is water, which is not considered hazardous under US and EU
regulations or GHS guidelines.
300-7570 Rev C Page 3
Material Safety Data Sheet Ba 4-Plex Assay
Effective Date: March 8th, 2010
Supersedes: April 22nd, 2009
Reagent 2 contains the following hazardous ingredients:
EINECs / % Composition EU Risk Phrase
Ingredient CAS#
ELINCS #
Sodium azide 26628-22-8 247-852-1 0.05 R 28-32-50/53
All other ingredients in Reagent 2 are either non-hazardous under US and EU regulations or GHS
guidelines and/or at concentrations less than 1% in the mixture.
4. First Aid Measures
For all components:
Immediately flush eyes thoroughly with water and notify supervisor, EHS personnel and
Eye:
medical personnel.
Immediately flush thoroughly with water and notify supervisor, EHS and medical
Skin:
personnel.
If swallowed, wash out mouth with water provided person is conscious. Never give
Ingestion:
anything by mouth to an unconscious person. Get medical attention. Do not induce
vomiting unless directed to do so by medical personnel.
Remove to fresh air and get medical attention for any breathing difficulty.
Inhalation:
5. Fire Fighting Measures
Reagent 2 contains sodium azide which has the potential to react
Flammability/Explosivity:
with copper, lead, brass, or solder in the plumbing system to form
an accumulation of lead and/or copper azide which is potentially
explosive.
All other components are not flammable or explosive in the
physical form and concentration in each component.
CO2, multipurpose dry chemical or vaporizing liquid fire
Extinguishing Media:
extinguishers are preferred extinguishing media to extinguish the
flammable liquid used in case of a fire.
Wear full protective clothing and a self-contained breathing
Special Fire Fighting Procedures:
apparatus with a full facepiece operated in the pressure demand
or other positive pressure mode for surrounding fire.
Decontaminate all equipment after use.
Hazardous Decomposition Products: Reagent 1 may release small quantities of chlorine in case of a
fire. Reagent 2 may release small quantities of azide in case of a
300-7570 Rev C Page 4
Material Safety Data Sheet Ba 4-Plex Assay
Effective Date: March 8th, 2010
Supersedes: April 22nd, 2009
fire. Other components may release small quantities of oxides of
carbon in case of a fire.
6. Accidental Release Measures
For all components if spilled:
If product/material is released or spilled, take proper precautions to minimize exposure by using
appropriate personal protective equipment. For small spills, wear gloves and absorb spill with paper
towel. For liquid products, avoid skin and eye contact by using rubber or nitrile gloves and wearing
of eye protection to avoid contact. Dispose of material according to local, State and Federal waste
disposal regulations (see Section 13).
For larger spills, wear personal protective clothing to minimize exposure such as overgarment, gloves and
eye protection (goggles), cover spill with absorbent material. For larger spills, wear overgarment and
personal protective equipment to avoid skin contact including goggles and rubber or nitrile gloves.
Collect spilled material, absorbent, and rinse waters into suitable containers for proper disposal in
accordance with applicable local, state or Federal waste disposal regulations (see Section 13).
7. Handling and Storage
Avoid skin contact, eye contact and inhalation.
Handling Precautions:
Store according to product labeling.
Storage Requirements:
8. Exposure Controls/Personal Protection
Occupational Exposure
Sodium azide is an ingredient in Reagent 2 and has the following
Limits:
exposure limits: ACGIH TLV Ceiling of 0.29 mg/m3 for all forms and
ceiling 0.11 ppm as hydrazoic acid vapor; and NIOSH has a REL of 0.3
mg/m3 for all forms. None of the other ingredients contained in this
product have occupational exposure limits established by OSHA,
NIOSH, ACGIH or Cepheid.
300-7570 Rev C Page 5
Material Safety Data Sheet Ba 4-Plex Assay
Effective Date: March 8th, 2010
Supersedes: April 22nd, 2009
None normally required. When practicable, handle material in enclosed
Engineering Controls:
or contained processes or in processes with effective local exhaust
ventilation.
Wear safety glasses with side shields, chemical splash goggles, or full face
Eye Protection:
shield, if necessary. Base the choice of protection on the job activity and
potential for contact with eyes or face.
Respiratory Protection: When possible, handle material in enclosed processes or containers. If it is
properly handled with effective ventilation or containment, respiratory protection
should not be needed.
Rubber gloves are recommended to minimize potential for skin contact. In
Skin Protection:
laboratory setting, wear lab coat or other protective overgarment at a minimum to
minimize skin contact. Base the choice of protection on the job activity and
potential for skin contact.
Facilities storing or using this product should be equipped with an eyewash
Other:
station and a safety shower. Wash hands, face and other potentially exposed
areas immediately after handling material (especially before eating, drinking, or
smoking). Decontaminate all protective equipment after use.
9. Physical and Chemical Properties
Physical State: Beads are solid components in cartridges; reagents are liquids.
Odor: Components are odorless.
Vapor Pressure: With the exception of Reagent 1 which may release small quantities of chlorine,
minimal.
Evaporation Rate: With the exception of Reagent 1, which may release small quantities of chlorine,
minimal.
Viscosity: Reagents are aqueous; beads are freeze dried powders.
Boiling Point: Liquid reagent boiling point is near 100 degrees C.
Freezing/Melting Point: Liquid reagents near 0 degrees C
Solubility: Reagents are already aqueous; beads are soluble in aqueous solutions
pH: 9-9.5 (Reagent 1) 8-9 (Reagent 2); 7-9 (solids)
300-7570 Rev C Page 6
Material Safety Data Sheet Ba 4-Plex Assay
Effective Date: March 8th, 2010
Supersedes: April 22nd, 2009
10. Stability and Reactivity
Stable under normal temperatures and pressures.
Chemical Stability:
Reagent 2 contains sodium azide, which at the concentration contained
Conditions to Avoid:
in the product may liberate gas if contacting acids or if allowed to
accumulate in metal piping cause an explosive reaction.
Incompatibilities with Other materials: Strong oxidizing agents, peroxides, strong acids and bases,
acid chlorides, acid anhydrides, alkali metals, ammonia.
Hazardous Decomposition Products: Carbon monoxide, carbon dioxide, nitrogen oxides, sulfur oxides,
sodium oxides, potassium oxides, hydrogen chloride, cyanide.
Hazardous Polymerization: Will not occur.
11. Toxicological Information
Acute Toxicity:
The cartridges containing freeze-dried powders should not present an acute toxicity hazard unless the
beads are released or spilled. Sodium azide is acutely toxic both by oral and dermal routes with an oral
LD50 values in the rat of 27 mg/kg and a dermal LD50 in the rabbit of 20 mg/kg. Magnesium chloride has
low to moderate acute toxicity with oral LD50 of 2800 mg/kg in the rat. Tween 20 is not considered
acutely toxic.
Irritation/Sensitization:
HEPES Salt and HEPES acid are considered skin and eye irritants. Bovine serum albumin, as a foreign
protein is considered a potential allergen but because it is contained in the bead within the cartridge, the
potential to cause an allergic reaction under normal use conditions is considered low. Tween 20 is
considered an irritant to the skin and eyes if contacted and respiratory tract if inhaled.
Repeated dose toxicity:
Administration of sodium azide has been shown to produce hypotension in laboratory animals and
humans. No other data identified on ingredients in beads or liquid formulations.
Reproductive (fertility) and Developmental (birth defects) toxicity ?None of the ingredients are
considered reproductive or developmental toxicants.
Mutagenicity and Carcinogenicity ?Sodium azide has positive mutagenic properties, primarily due to
its active azide group; the relevance of this result for human health is not fully known. None of the
ingredients are listed by NTP, IARC or OSHA as carcinogens.
300-7570 Rev C Page 7
Material Safety Data Sheet Ba 4-Plex Assay
Effective Date: March 8th, 2010
Supersedes: April 22nd, 2009
12. Ecological Information
Ecotoxicity: Sodium azide is considered toxic to aquatic organisms. No other data identified.
Environmental Fate: No data identified.
13. Disposal Considerations
Biological specimens, including used cartridges, should be treated as capable of transmitting infectious
agents. Consult your institution's environmental waste personnel on proper disposal of used cartridges
and unused reagents. This material may exhibit characteristics of federal EPA Resource Conservation and
Recovery Act (RCRA) hazardous waste requiring specific disposal requirements. Check state and local
regulations as they may differ from federal disposal regulations. Institutions outside the USA should
check their country hazardous waste disposal requirements.
14. Transport Information
Transport in accordance with all federal, state, and local transportation regulations.
None of the components are regulated by US DOT or IATA.
15. Regulatory Information
This MSDS complies with the requirements under 29 CFR 1910.1200
US OSHA:
Certain components of the kit require labeling under either or both of US and EU regulations and
therefore labeling of this product should contain the following:
300-7570 Rev C Page 8
Material Safety Data Sheet Ba 4-Plex Assay
Effective Date: March 8th, 2010
Supersedes: April 22nd, 2009
Reagent 1
CAUTION (Contains sodium hypochlorite)
Irritating to eyes.
Avoid skin and eye contact.
EU Risk and Safety Phrases: R 36; S24/25
Reagent 2
CAUTION (Contains sodium azide)
Contact with acids liberates very toxic gas.
Harmful to aquatic organisms; may cause long-term adverse
effects in the aquatic environment.
Avoid release to the environment; Refer to special
instructions/safety data sheets
EU Risk and Safety Phrases: R 32; 52/53; S61
Canada ?WHMIS Classifications
All components are not classified according to WHMIS classification criteria.
California Proposition 65
None of the ingredients are listed under California Proposition 65.
Not listed.
SARA 313:
None of the ingredients are listed under CERCLA.
CERCLA :
None of the ingredients are listed under RCRA.
RCRA:
For R&D consumers, products are to be used only for R&D purposes.
16. Other Information
No other data available.
Abbreviations:
ACGIH: American Conference of Governmental Industrial Hygienists
CAS#: Chemical Abstract Services Number
CFR Code of Federal Regulations
CERCLA: Comprehensive Environmental Response, Compensation, and Liability Act
DOT: Department of Transportation
EINECS: European Inventory of New and Existing Chemical Substances
EU: European Union
GHS: Global Harmonization System
IARC: International Agency for Research on Cancer
300-7570 Rev C Page 9
Material Safety Data Sheet Ba 4-Plex Assay
Effective Date: March 8th, 2010
Supersedes: April 22nd, 2009
IATA: International Air Transport Association
OSHA: Occupational Safety and Health Administration
NTP: National Toxicology Program
RCRA: Resource Conservation and Recovery Act
SARA: Superfund Amendments and Reauthorization Act
TSCA: Toxic Substances Control Act
Cepheid makes no warranties as to the accuracy or completeness of this information and
disclaims any liability in connection with its use. Cepheid's obligations shall be only as set forth
in Cepheid's standard terms and conditions of sale for this product. In no case will Cepheid be
liable for any incidental, indirect or consequential damages arising out of the sale, resale, use or
misuse of the product.
300-7570 Rev C Page 10
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