Burdick & Jackson
Material Safety Data Sheet
Detritylation
(2.5% Dichloroacetic acid in Dichloromethane (Methylene Chloride), v/v)
1. CHEMICAL PRODUCT AND COMPANY IDENTIFICATION
PRODUCT NAME: Detritylation
PRODUCT CORE NUMBER 619
OTHER/GENERIC NAMES: Detrit, Deblock, Deblock solution, Debolck-D, Deblocking mix, Deblock mix,
Detritylation mix, Detritylation solution, DMT removal agent, MC deblock, and
DCA/DCM
PRODUCT USE: A DNA Synthesis Reagent
MANUFACTURER: Honeywell, Burdick & Jackson
1953 South Harvey Street
Muskegon, MI 49442
FOR MORE INFORMATION CALL: IN CASE OF EMERGENCY CALL:
(Monday-Friday, 8:00am-5:00pm) (24 Hours/Day, 7 Days/Week)
1-800-368-0050 1-800-707-4555 or Chemtrec 1-800-424-9300
2. COMPOSITION/INFORMATION ON INGREDIENTS
INGREDIENT NAME CAS NUMBER WEIGHT %
Methylene Chloride (Dichloromethane) 75-09-2 97.0%
Dichloroacetic Acid 79-43-6 3.0%
Trace impurities and additional material names not listed above may also appear in Section 15 towards the end of the
MSDS. These materials may be listed for local "Right-To-Know" compliance and for other reasons.
3. HAZARDS IDENTIFICATION
EMERGENCY OVERVIEW: Corrosive! Toxic! Can cause severe burns by all routes of exposure.
Components are suspected carcinogens. Methylene chloride is also highly toxic. Exposure effects may result
from inhalation, ingestion, skin contact and skin absorption. Non-flammable.
POTENTIAL HEALTH HAZARDS
SKIN: Can cause skin burns and irritation. Toxic by skin absorption.
EYES: Can cause burns, irritation and pain. Corneal injury is possible.
INHALATION: Inhalation of liquid, vapor or fumes can be destructive to the respiratory tract.
Inhalation toxic effects include fatigue, nausea, light-headedness and mental confusion.
Unconsciousness and death can also result from extreme cases of overexposure.
Breathing vapors can elevate carboxyhemoglobin levels in the cardiovascular system. .
Persons who smoke tobacco products will experience an intensified elevation of
MSDS Number: DNA 0619 Page 1 of 8
Current Issue Date: November, 2004
Burdick & Jackson
MATERIAL SAFETY DATA SHEET
Detritylation (DCA in DCM)
carboxyhemoglobin levels.
INGESTION: Can cause burns and do damage to throat and mouth tissue. Absorption of the toxic component
may also result in absorption producing blood, central nervous system and cardiovascular effects.
DELAYED EFFECTS: Chronic exposure may have cardiovascular, liver, kidney effects. Depression, mental
confusion, loss of appetite and visual disturbances may also occur. Components are
considered to be suspect carcinogens.
Ingredients that are found on one of the OSHA designated carcinogen lists are listed below.
INGREDIENT NAME NTP STATUS IARC STATUS OSHA LIST
Dichloromethane Anticipated carcinogen 2B -
Dichloroacetic acid - 2B -
Methylene Chloride is classified A3, confirmed animal carcinogen with unknown relevance to humans, by ACGIH.
Dichloroacetic acid is classified A3, confirmed animal carcinogen with unknown relevance to humans, by ACGIH.
4. FIRST AID MEASURES
SKIN: Rinse affected areas with water for at least 15 minutes. Remove any contaminated clothing.
Obtain professional medical assistance immediately.
EYES: Rinse eyes with plenty of water for at least 15 minutes. Obtain professional medical assistance
immediately.
INHALATION: Remove from exposure area to fresh air. If victim is not breathing administer artificial respiration
according to your level of training and obtain professional medical assistance immediately
INGESTION: Do not induce vomiting unless directed to do so by a physician. Obtain professional medical
assistance immediately.
ADVICE TO PHYSICIAN: Treat symptomatically.
5. FIRE FIGHTING MEASURES
FLAMMABLE PROPERTIES
FLASH POINT: >210癋 (98.9癈) (Dichloroacetic acid)
FLASH POINT METHOD: Tag Closed-cup
AUTOIGNITION TEMPERATURE: ~ 1033癋 (556癈) (Dichloromethane)
UPPER FLAME LIMIT (volume % in air): ~19% (Dichloromethane)
LOWER FLAME LIMIT (volume % in air): ~12% (Dichloromethane)
FLAME PROPAGATION RATE (solids): Not Applicable
OSHA FLAMMABILITY CLASS: Not applicable
MSDS Number: DNA 0619 Page 2 of 8
Current Issue Date: November, 2004
Burdick & Jackson
MATERIAL SAFETY DATA SHEET
Detritylation (DCA in DCM)
EXTINGUISHING MEDIA:
Dry chemical or carbon dioxide
UNUSUAL FIRE AND EXPLOSION HAZARDS:
Very high vapor pressure. Containers may rupture if exposed to extreme heat or fire conditions. Will form
flammable vapor-air mixtures at 100癈 (212癋) and higher.
SPECIAL FIRE FIGHTING PRECAUTIONS/INSTRUCTIONS:
Wear self-contained breathing apparatus.
6. ACCIDENTAL RELEASE MEASURES
IN CASES OF SPILL OR OTHER RELEASE: (Always wear recommended personal protective equipment.)
Isolate the spill area. Stop leak in a safe and practical manner. (If leak cannot be stopped easily and safely, advise
trained emergency response personnel of the situation.) Using inert material (such as ground corncobs) dike the
spilled solvent to prevent it from running into drains or waterways. Place absorbed material in a compatible
leak-proof container for disposition by persons trained in the handling of hazardous substances
Spills and releases may have to be reported to Federal and/or local authorities. See Section 15 regarding reporting
requirements.
7. HANDLING AND STORAGE
NORMAL HANDLING: (Always wear recommended personal protective equipment.)
Do not use in unventilated areas. Avoid breathing vapors. Avoid contact with skin and eyes. Wear neoprene,
polyvinyl chloride, or other solvent resistant gloves, apron and boots.
STORAGE RECOMMENDATIONS:
Store in closed containers in an area designed for corrosive substances. Protect from temperature extremes and
from light.
8. EXPOSURE CONTROLS/PERSONAL PROTECTION
ENGINEERING CONTROLS:
Provide general or local exhaust ventilation systems to maintain airborne concentrations below exposure levels.
Local exhaust ventilation is preferred because it prevents contaminant dispersion into the work area by
controlling it at its source.
MSDS Number: DNA 0619 Page 3 of 8
Current Issue Date: November, 2004
Burdick & Jackson
MATERIAL SAFETY DATA SHEET
Detritylation (DCA in DCM)
PERSONAL PROTECTIVE EQUIPMENT
SKIN PROTECTION:
Where liquid contact with exposed skin is possible, wear solvent resistant impervious personal protective
equipment (gloves, face shields, etc.). Contaminated clothing and PPE should be laundered and cleaned
separately at the work site.
EYE PROTECTION:
Wear safety glasses. Contact lenses should not be worn. Where splashing or dripping is likely to occur, use
chemical resistant goggles and/or face shield.
RESPIRATORY PROTECTION:
Where the airborne contamination and subsequent employee exposure cannot be maintained below the
exposure level, NIOSH approved supplied breathing air systems are required. Air purifying respirators
(charcoal canister type) are authorized only for emergency escape.
For complete details concerning respiratory protection for methylene chloride see 29 CFR 1910.1052.
ADDITIONAL RECOMMENDATIONS:
Provide safety showers and eyewash stations.
EXPOSURE GUIDELINES
INGREDIENT NAME ACGIH TLV OSHA PEL OTHER LIMIT
Dichloromethane 50 ppm TWA 25 ppm TWA - skin None
125 ppm STEL - skin
Dichloroacetic Acid 0.5 ppm None None
* = Limit established by Honeywell International, Inc.
** = Workplace Environmental Exposure Level (AIHA).
*** = Biological Exposure Index (ACGIH).
OTHER EXPOSURE LIMITS FOR POTENTIAL DECOMPOSITION PRODUCTS:
None
9. PHYSICAL AND CHEMICAL PROPERTIES
APPEARANCE: Clear, colorless
PHYSICAL STATE: Liquid
MOLECULAR WEIGHT: 84.93 (Dichloromethane); 129 (Dichloroacetic Acid)
CHEMICAL FORMULA: CH2Cl2 (Dichloromethane); Cl2CHCOOH (Dichloroacetic Acid)
ODOR: Mild, sweet (Similar to Chloroform)
SPECIFIC GRAVITY (water = 1.0): 1.33 gm/cc @ 20癈
SOLUBILITY IN WATER (weight %): 1.32gm/100gm @ 25癈 (Dichloromethane); Dichloroacetic Acid is miscible
pH: for this mixture 2 (1% Solution in water)
BOILING POINT: 40癈 (104癋) Dichloromethane; 194癈 (381癋) Dichloroacetic Acid
MELTING POINT: ?5癈 (20.8癋) Dichloromethane; 12癈 (53.6癋) Dichloroacetic Acid
MSDS Number: DNA 0619 Page 4 of 8
Current Issue Date: November, 2004
Burdick & Jackson
MATERIAL SAFETY DATA SHEET
Detritylation (DCA in DCM)
VAPOR PRESSURE: 350mm Hg 68癋 (Dichloromethane); 0.19mm Hg @ 68癋 (Dichloroacetic
Acid)
VAPOR DENSITY (air = 1.0): 2.9
EVAPORATION RATE: 0.7 COMPARED TO: Ether =1
% VOLATILES: 96.5%
FLASH POINT: >210癋 (98.9癈)
(Flash point method and additional flammability data are found in Section 5.)
10. STABILITY AND REACTIVITY
NORMALLY STABLE? (CONDITIONS TO AVOID):
Stable
INCOMPATIBILITIES:
Aluminum, magnesium and strong alkaline solutions. Aluminum and magnesium can react with methylene
chloride under some circumstances, especially if exposed over a long time period.
HAZARDOUS DECOMPOSITION PRODUCTS:
Avoid open flame or hot surfaces, which can cause thermal decomposition.
Toxic and corrosive gases of phosgene, hydrogen chloride, and/or carbon monoxide can form.
HAZARDOUS POLYMERIZATION:
Will not occur.
11. TOXICOLOGICAL INFORMATION
For Dichloromethane
IMMEDIATE (ACUTE) EFFECTS:
Rat, oral, LD50: 1600 mg/kg
Mouse, inhalation, LC50: 14,400 ppm/7 hrs.
Rabbit, eye: moderate irritant (162 mg)
Rabbit, skin: moderate irritant (100 mg/24 hr.)
DELAYED (SUBCHRONIC AND CHRONIC) EFFECTS:
Can cause damage to liver and kidneys. Suspected carcinogen based on 2-yr. bioassay in rats that showed liver
tumors.
OTHER DATA:
Mutagenicity: mutagenic in salmonella typhimurium Ames assay
: gene mutation in hamster ovary cells t 3,000 ppm
: DNA damage in mouse liver cells (400 u mol/L)
MSDS Number: DNA 0619 Page 5 of 8
Current Issue Date: November, 2004
Burdick & Jackson
MATERIAL SAFETY DATA SHEET
Detritylation (DCA in DCM)
For Dichloroacetic Acid
IMMEDIATE (ACUTE) EFFECTS:
Oral (rat) LD50: 2820 mg/kg
Dermal (rabbit) LD50: 510 ul/kg
Rabbit skin: 2 mg/24 hr. - Severe irritation
Rabbit eye: 50 ug open - Severe irritation
DELAYED (SUBCHRONIC AND CHRONIC) EFFECTS:
Animal data has shown reproductive effects (fetotoxicity). Possible carcinogen.
OTHER DATA:
None
12. ECOLOGICAL INFORMATION
LC50 (Fathead minnow): 310 mg/L/96 hr., static
LC50 (Fathead minnow): 193 mg/L/96 hr., flow-through
LC50 (Daphnia magna): 224 mg/L/48 hr
13. DISPOSAL CONSIDERATIONS
RCRA
Is the unused product a RCRA hazardous waste if discarded? Yes
If yes, the RCRA ID number is: U080, D002
OTHER DISPOSAL CONSIDERATIONS:
Dispose of in accordance with local, state and federal regulations.
The information offered here is for the product as shipped. Use and/or alterations to the product such as mixing
with other materials may significantly change the characteristics of the material and alter the RCRA classification and
the proper disposal method.
14. TRANSPORT INFORMATION
US DOT PROPER SHIPPING NAME: Corrosive liquids, toxic, n.o.s. (Dichloroacetic acid & Dichloromethane)
US DOT HAZARD CLASS: 8, Corrosive (Primary), 6.1, Toxic (Secondary)
US DOT ID NUMBER: UN 2922
US DOT PACKING GROUP: II
NA EMERGENCY RESPONSE GUIDE: 154
For additional information on shipping regulations affecting this material, contact the information number found in
Section 1.
MSDS Number: DNA 0619 Page 6 of 8
Current Issue Date: November, 2004
Burdick & Jackson
MATERIAL SAFETY DATA SHEET
Detritylation (DCA in DCM)
15. REGULATORY INFORMATION
TOXIC SUBSTANCES CONTROL ACT (TSCA)
TSCA INVENTORY STATUS: The components of this mixture are listed on the TSCA inventory.
OTHER TSCA ISSUES: Additional TSCA related information may exist. Contact Honeywell if you have
questions regarding your application or use of this product.
SARA TITLE III/CERCLA
"Reportable Quantities" (RQs) and/or "Threshold Planning Quantities" (TPQs) exist for the following ingredients.
INGREDIENT NAME SARA/CERCLA RQ (lb) SARA EHS TPQ (lb)
Dichloromethane 1000 Not Listed
Dichloroacetic acid
Spills or releases resulting in the loss of any ingredient at or above its RQ requires immediate notification to
the National Response Center [(800) 424-8802] and to your Local Emergency Planning Committee.
SECTION 311 HAZARD CLASS: Immediate, Delayed
SARA 313 TOXIC CHEMICALS:
The following ingredients are SARA 313 "Toxic Chemicals". CAS numbers and weight percents are found in
Section 2.
INGREDIENT NAME COMMENT
Dichloromethane Deminimis concentration is 0.1%
STATE RIGHT-TO-KNOW
In addition to the ingredients found in Section 2, the following are listed for state right-to-know purposes.
INGREDIENT NAME WEIGHT % COMMENT
None Listed
ADDITIONAL REGULATORY INFORMATION:
California Proposition 65 Label Statement
Methylene Chloride is listed on the California Proposition 65 Chemicals Known to the State to Cause Cancer list; therefore, the
following statement has been placed on the product label:
"Warning: This product contains a chemical known to the State of California to cause cancer."
Dichloroacetic Acid is listed on the California Proposition 65 Chemicals Known to the State to Cause Cancer list; therefore, the
following statement has been placed on the product label:
"Warning: This product contains a chemical known to the State of California to cause cancer."
MSDS Number: DNA 0619 Page 7 of 8
Current Issue Date: November, 2004
Burdick & Jackson
MATERIAL SAFETY DATA SHEET
Detritylation (DCA in DCM)
WHMIS CLASSIFICATION (CANADA):
D-1B based on TDG Classification for Dichloromethane
FOREIGN INVENTORY STATUS:
All of the components are listed on the following foreign inventories: Australia, Canada, China, the European Union,
Japan, Korea and the Philippines.
16. OTHER INFORMATION
CURRENT ISSUE DATE: November 2004
PREVIOUS ISSUE DATE: None
CHANGES TO MSDS FROM PREVIOUS ISSUE DATE ARE DUE TO THE FOLLOWING:
New MSDS
NFPA Classification (Dichloromethane)
Health: 3
Flammability: 1
Instability: 0
MSDS Number: DNA 0619 Page 8 of 8
Current Issue Date: November, 2004
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