螠 University Clinic Heidelberg
Manual No.:100B
Department of Transplantation Immunology
Revision: August 21, 2006
Im Neuenheimer Feld 305
D-69120 Heidelberg - Germany
Phone: +49-6221-564013
Fax: +49-6221-564200
www.ctstransplant.org
CTS Collaborative Transplant Study
Material Safety Data Sheet for the
CTS-PCR-SSP TRAY and MINITRAY KITS
1. Identification of the products and company
1.1 Products
To be applied to the following products:
Product No. Description
101 HLA-A* CTS-PCR-SSP TRAY KIT
102 HLA-B* CTS-PCR-SSP TRAY KIT
104 HLA-DRB1* CTS-PCR-SSP TRAY KIT
120 HLA-A*+B*+Cw* CTS-PCR-SSP TRAY KIT
121 HLA-A*+B*+DRB1* CTS-PCR-SSP TRAY KIT
122 HLA-DRB1*+DQB1* Low Resolution CTS-PCR-SSP TRAY KIT
226 HLA-DRB High Resolution CTS-PCR-SSP MINITRAY KIT
207 HLA-DRB1*01 Subtyping CTS-PCR-SSP MINITRAY KIT
208 HLA-DRB1*03 Subtyping CTS-PCR-SSP MINITRAY KIT
IVD
209 HLA-DRB1*04 Subtyping CTS-PCR-SSP MINITRAY KIT
210 HLA-DRB1*07/09 Subtyping CTS-PCR-SSP MINITRAY KIT
211 HLA-DRB1*08/12 Subtyping CTS-PCR-SSP MINITRAY KIT
212 HLA-DRB1*11 Subtyping CTS-PCR-SSP MINITRAY KIT
213 HLA-DRB1*13 Subtyping CTS-PCR-SSP MINITRAY KIT
214 HLA-DRB1*14 Subtyping CTS-PCR-SSP MINITRAY KIT
215 HLA-DRB1*15/16 Subtyping CTS-PCR-SSP MINITRAY KIT
103 HLA-Cw* CTS-PCR-SSP TRAY KIT
119 HLA-DQB1*Low Resolution CTS-PCR-SSP TRAY KIT
127 HLA-DQA1* Low Resolution CTS-PCR-SSP TRAY KIT
105 HLA-DQB1* High Resolution CTS-PCR-SSP TRAY KIT
130 HLA-DQB1* High Resolution (Part I) CTS-PCR-SSP TRAY KIT
HLA-DQB1* High Resolution (PartII = DQB1*06 Subtyping)
131
CTS-PCR-SSP TRAY KIT
106 HLA-DQA1* High Resolution CTS-PCR-SSP TRAY KIT
128 HLA-DQB1*+DQA1* Low Resolution CTS-PCR-SSP TRAY KIT
123 HLA-DQB1*+DQA1* High Resolution CTS-PCR-SSP TRAY KIT
124 Cytokine CTS-PCR-SSP TRAY KIT
225 HPA CTS-PCR-SSP MINITRAY KIT
502 CTS-Cycler Control Kit
229 mHA CTS-PCR-SSP MINITRAY KIT For research use only
1.2 Company: University Clinic Heidelberg - Department of Transplantation Immunology
Im Neuenheimer Feld 305 - D-69120 Heidelberg - Germany
Phone: +49-6221-564013 - Fax: +49-6221-564200
1.3 In case of emergency: Call your local fire department
Manual No.: 100B Page 1 of 5 MSDS Tray and Minitray Kits
Rev.: May 12, 2006
2. Composition/information on ingredients
Common
Component Chemical CAS No. R S
names
Cresol red
62625-29-0 none none
PCR
170274-78-9
Deoxyribonucleic acid Oligonucleotides
primer mixes
none none
(= DNA)
Ammonium sulfate 7783-20-2 R36/37/38 S26/36
77-86-1,
Tris buffer R36/37/38 S26/36
1185-53-1
7791-18-6
Magnesium chloride none S26
56-81-5
Glycerol Glycerin none S23-25
Mastermix
Cresol red 62625-29-0 none none
dNTPs
Deoxyribonucleotides 1927-31-7 none none
Cas. No: unique numeric identifier (designates only one substance)
R-Phrases: health risks
S-Phrases: safety precautions
3. Hazards identification
Harmful if swallowed (cresol red)
Irritating to eyes, respiratory system, mucous membranes and skin (Tris Buffer)
Target organ: eyes, nerves, kidney, central nervous system
In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Wear suitable protective clothing.
4. First aid measures
In case of contact with eyes: immediately flush eyes with copious amounts of water for at least 15
minutes. Call a physician.
In case of contact with skin: immediately wash skin with soap and copious amounts of water. Call a
physician.
Manual No.: 100B Page 2 of 5 MSDS Tray and Minitray Kits
Rev.: August 21, 2006
In case of inhalation: remove to fresh air, if not breathing give artificial respiration. If breathing
difficult, give oxygen. Call a physician.
In case of ingestion: wash out mouth with water provided person is conscious. Call a physician.
Remove and wash contaminated clothing before reuse.
5. Fire-fighting measures
Extinguishing media: water spray, carbon dioxide, dry chemical powder or appropriate foam.
Special fire fighting procedures: wear self-contained breathing apparatus and protective clothing to
prevent contact with skin and eyes.
Unusual fire hazards: emits toxic fumes under fire conditions.
6. Accidental release measures
Personal protection: wear respirator, chemical safety goggles, rubber boots and heavy rubber gloves.
Cleaning method: contain spill, absorb with inert material.
Sweep up, avoid raising dust, place in a suitable container and hold for disposal.
Ventilate area and wash spill site after material pick up.
7. Handling and storage
Wear suitable protective clothing and gloves. It is not necessary to work in a hood or to wear a mask
while performing the test.
Store at temperature indicated on the product labels and the working instruction.
8. Exposure controls/personal protection
Chemical safety goggles, compatible chemical-resistant gloves, NIOSH/MSHA-approved respirator,
safety shower and eye bath.
Do not inhale. Mechanical exhaust is required.
Irritant. Avoid contact with skin, eyes, mucous membranes and clothing.
Manual No.: 100B Page 3 of 5 MSDS Tray and Minitray Kits
Rev.: August 21, 2006
Wash thoroughly after handling.
Keep tightly closed.
9. Physical and chemical properties
Component Appearance Color
CTS-PCR-SSP
Lyophilized solution pink
TRAY/MINITRAY KIT
Mastermix slight viscous liquid purple
Additional information:
- The addition of sodium hypochlorite to a solution of acidified ammonium sulfate led to the formation
of nitrogen trichloride and resulted in an explosion.
- Contact of potassium or sodium-potassium alloy with a mixture of ammonium sulfate and ammonium
nitrate results in an explosion.
- Heating a mixture of potassium chlorate and ammonium sulfate results in decomposition with
incandescence.
- Mixing ammonium sulfate with fused potassium nitrate results in a vigorous reaction with flames.
Decomposes at 235掳C releasing ammonia and sulfur.
- Violent or explosive reactions have occurred upon direct contact of glycerol with: sodium hydride,
phosphorous trioxide, perchloric acid, chlorine, calciumhypochlorite, nitric acid and hydrofluoric acid,
nitric acid and sulfuric acid, sodium peroxide, hydrogen peroxide or potassium permanganate.
10. Stability and reactivity
Incompatibilities: strong oxidizing agents, strong bases
Hazardous combustion or decomposition products: sulfur oxides, ammonia, nitrogen oxides, carbon
monoxide, carbon dioxide, and phosphorous oxides
Hazardous polymerization: will not occur
Manual No.: 100B Page 4 of 5 MSDS Tray and Minitray Kits
Rev.: August 21, 2006
11. Toxicological information
Acute effects: harmful if swallowed. May be harmful if inhaled. May be harmful if absorbed through
skin. Causes eye and skin irritation. Material is irritating to mucous membranes and upper respiratory
tract. Can cause CNS depression
To the best of our knowledge the chemical, physical and toxicological properties have not been
thoroughly investigated.
12. Ecological information
Data not available.
13. Disposal considerations
Dissolve or mix the material with a combustible solvent and burn in a chemical incinerator equipped
with an afterburner and scrubber. Observe all Federal, State and Local environmental regulations.
14. Transport information
Contact dna-labor@med.uni-heidelberg.de for transportation information.
15. Regulatory information
Information according to EEC directives:
R phrases: R 36 /37/38
S phrases: S 26/36
S 26
S23-24/25
16. Other information
The above information is believed to be accurate but does not purport to be comprehensive and should
only be used as a guide. This product is for professional use by PCR-trained personnel only. The
Department of Transplantation Immunology, University Clinic Heidelberg, is not liable for any damage
resulting from handling or contact with the products mentioned above. See the information in the
product catalogue or packing slip for additional terms and conditions of sale.
Manual No.: 100B Page 5 of 5 MSDS Tray and Minitray Kits
Rev.: August 21, 2006
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