University Clinic Heidelberg
Manual No.: 400B
Department of Transplantation Immunology
Revision: April 03, 2007
Im Neuenheimer Feld 305
D-69120 Heidelberg - Germany
Phone: +49-6221-564013
Fax: +49-6221-564200
www.ctstransplant.org
CTS Collaborative Transplant Study
Material Safety Data Sheet for the
CTS-PCR-SSP BULK KITS
1. Identification of the products and company
1.1 Products
Description
Product No.
401 HLA-A* CTS-PCR-SSP BULK KIT (24 mixes)
402 HLA-B* CTS-PCR-SSP BULK KIT (48 mixes)
0197
404 HLA-DRB1* CTS-PCR-SSP BULK KIT (24 mixes)
403 HLA-Cw* CTS-PCR-SSP BULK KIT (24 mixes)
IVD
1.2 Company: University Clinic Heidelberg
Department of Transplantation Immunology
Im Neuenheimer Feld 305
D-69120 Heidelberg
Germany
Phone: +49-6221-564013
Fax: +49-6221-564200
1.3 In case of emergency: Call your local fire department.
Manual No.: 400B 1/5 MSDS Bulk Kits
Rev.: April 3, 2007
2. Composition/information on ingredients
Common
Component Chemical CAS No. R S
names
Cresol red
62625-29-0 none none
BULK
170274-78-9
Deoxyribonucleic Oligonucleotides
reagents
none none
(PCR primer acid (= DNA)
mixes)
Ammonium
7783-20-2 R36/37/38 S26/36
sulfate
77-86-1,
Tris buffer 1185-53-1 R36/37/38 S26/36
Magnesium 7791-18-6
none S26
chloride
Mastermix 56-81-5
Glycerol Glycerin none S23-25
Cresol red 62625-29-0 none none
Deoxy- dNTPs
1927-31-7 none none
ribonucleotides
CAS No.: unique numeric identifier (designates only one substance)
R-Phrases: health risks
S-Phrases: safety precautions
3. Hazards identification
Harmful if swallowed (cresol red)
Irritating to eyes, respiratory system, mucous membranes and skin (Tris Buffer)
Target organ: eyes, nerves, kidney, central nervous system
In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Wear suitable protective clothing.
4. First aid measures
In case of contact with eyes: immediately flush eyes with copious amounts of water for at least 15
minutes. Call a physician.
In case of contact with skin: immediately wash skin with soap and copious amounts of water. Call a
physician.
Manual No.: 400B 2/5 MSDS Bulk Kits
Rev.: April 3, 2007
In case of inhalation: remove to fresh air, if not breathing give artificial respiration. If breathing
difficult, give oxygen. Call a physician.
In case of ingestion: wash out mouth with water provided person is conscious. Call a physician.
Remove and wash contaminated clothing before reuse.
5. Fire-fighting measures
Extinguishing media: water spray, carbon dioxide, dry chemical powder or appropriate foam.
Special fire fighting procedures: wear self-contained breathing apparatus and protective clothing to
prevent contact with skin and eyes.
Unusual fire hazards: emits toxic fumes under fire conditions
6. Accidental release measures
Personal protection: wear respirator, chemical safety goggles, rubber boots and heavy rubber gloves.
Cleaning method: contain spill, absorb with inert material.
Sweep up, avoid raising dust, place in a suitable container and hold for disposal.
Ventilate area and wash spill site after material pick up.
7. Handling and storage
Wear suitable protective clothing and gloves. It is not necessary to work in a hood or to wear a mask
while performing the test.
Store at temperature indicated on the product labels and the working instruction.
8. Exposure controls/personal protection
Chemical safety goggles, compatible chemical-resistant gloves, NIOSH/MSHA-approved respirator,
safety shower and eye bath.
Do not inhale. Mechanical exhaust is required.
Irritant. Avoid contact with skin, eyes, mucous membranes and clothing.
Wash thoroughly after handling.
Keep tightly closed.
Manual No.: 400B 3/5 MSDS Bulk Kits
Rev.: April 3, 2007
9. Physical and chemical properties
Component Appearance Color
CTS-PCR-SSP BULK KIT frozen liquid pink
Mastermix slight viscous liquid purple
Additional information:
- The addition of sodium hypochlorite to a solution of acidified ammonium sulfate led to the formation
of nitrogen trichloride and resulted in an explosion.
- Contact of potassium or sodium-potassium alloy with a mixture of ammonium sulfate and ammonium
nitrate results in an explosion.
- Heating a mixture of potassium chlorate and ammonium sulfate results in decomposition with
incandescence.
- Mixing ammonium sulfate with fused potassium nitrate results in a vigorous reaction with flames.
Decomposes at 235掳C releasing ammonia and sulfur.
- Violent or explosive reactions have occurred upon direct contact of glycerol with: sodium hydride,
phosphorous trioxide, perchloric acid, chlorine, calciumhypochlorite, nitric acid and hydrofluoric acid,
nitric acid and sulfuric acid, sodium peroxide, hydrogen peroxide or potassium permanganate.
10. Stability and reactivity
Incompatibilities: strong oxidizing agents, strong bases
Hazardous combustion or decomposition products: sulfur oxides, ammonia, nitrogen oxides, carbon
monoxide, carbon dioxide, and phosphorous oxides
Hazardous polymerization: will not occur
11. Toxicological information
Acute effects: harmful if swallowed. May be harmful if inhaled or if absorbed through skin. Causes eye
and skin irritation. Material is irritating to mucous membranes and upper respiratory tract. Can cause
CNS depression
To the best of our knowledge the chemical, physical and toxicological properties have not been
thoroughly investigated.
12. Ecological information
Data not available.
Manual No.: 400B 4/5 MSDS Bulk Kits
Rev.: April 3, 2007
13. Disposal considerations
Dissolve or mix the material with a combustible solvent and burn in a chemical incinerator equipped
with an afterburner and scrubber. Observe all Federal, State and Local environmental regulations.
14. Transport information
Contact dna-labor@med.uni-heidelberg.de for transportation information.
15. Regulatory information
Information according to EEC directives:
R phrases: R 36 /37/38
S phrases: S 26/36
S 26
S23-24/25
16. Other information
The above information is believed to be accurate but does not purport to be comprehensive and should
only be used as a guide. This product is for professional use by PCR-trained personnel only. The
Department of Transplantation Immunology, University Clinic Heidelberg, is not liable for any damage
resulting from handling or contact with the products mentioned above. See the information in the
product catalogue or packing slip for additional terms and conditions of sale.
Manual No.: 400B 5/5 MSDS Bulk Kits
Rev.: April 3, 2007
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