Revision Date: 6/1/2006
MATERIAL SAFETY DATA SHEET
1. CHEMICAL PRODUCT AND COMPANY IDENTIFICATION
PRODUCT NAME: PRODUCT NUMBER: EC-835
SequaGel Buffer
CHEMICAL NAMES/ Solution of urea and buffer salts. Other ingredients are less than 1%.
DESCRIPTION:
MANUFACTURER: National Diagnostics, Inc. TELEPHONE NUMBER:
305 Patton Drive (800) 526-3867
Atlanta, GA 30336 (404) 699-2121
EMERGENCY NUMBER:
CHEMTREC (800) 424-9300
2. COMPOSITION / INFORMATION ON INGREDIENTS
Component % Comp CAS # EINECS # TLV (units)
Urea 40-50 57-13-6 200-315-5 10 mg/m3, 8-hour TWA
Boric Acid 5-10 10043-35-3 10 mg/m3 total dust
Tris-Base 10-20 77-86-1 201-064-4 none established
3. HAZARDS IDENTIFICATION
Clear colorless solution
APPEARANCE AND ODOR:
EMERGENCY OVERVIEW - IMMEDIATE HAZARD
Urea
CAUSES IRRITATION TO SKIN, EYES, AND RESPIRATORY TRACT. UREA IS HARMFUL IF SWALLOWED OR
INHALED.
Tris-Base
CAUSES IRRITATION TO SKIN, EYES, AND RESPIRATORY TRACT. HARMFUL IF SWALLOWED OR INHALED.
Boric Acid
CAUSES IRRITATION TO SKIN, EYES AND RESPIRATORY TRACT. BORIC ACID IS HARMFUL IF SWALLOWED
OR INHALED.
EMERGENCY OVERVIEW - CHRONIC HAZARD WARNING
Urea
LONG-TERM EXPOSURE TO HIGH AIRBORNE CONCENTRATIONS CAN LEAD TO PROTEIN METABOLISM
DISTURBANCES, MODERATE EMPHYSEMA, AND CHRONIC WEIGHT LOSS.
Tris-Base
CHRONIC DERMATITIS MAY FOLLOW SKIN CONTACT.
Boric Acid
PROLONGED ABSORPTION OF BORIC ACID CAUSES WEIGHT LOSS, VOMITING, DIARRHEA, SKIN RASH,
CONVULSIONS AND ANEMIA. AFFECTS CENTRAL NERVOUS SYSTEM, LIVER, AND KIDNEYS.
POTENTIAL HEALTH EFFECTS
INHALATION
Urea
Causes irritation to the respiratory tract.
Tris-Base
Causes irritation to the respiratory tract.
Boric Acid
Causes irritation to the mucous membranes of the respiratory tract.
INGESTION
Urea
Causes irritation to the garstrointestinal tract.
Tris-Base
Causes irritation and reddening to the mucous membranes of the mouth, esophagus, and gastrointestinal
tract.
Boric Acid
Harmful or fatal if ingested in sufficient volume.
SKIN
Urea
Page 1 SequaGel Buffer
Urea
Causes irritation to the skin.
Tris-Base
Causes irritation to the skin.
Boric Acid
Causes irritation to the skin.
EYES
Urea
Causes irritation to the eyes.
Tris-Base
Causes irritation to the eyes.
Boric Acid
Causes irritation to the eyes.
SIGNS AND SYMPTOMS 0F OVEREXPOSURE
INHALATION
Urea
Symptoms may include coughing, shortness of breath. May be absorbed into the bloodstream with symptoms
similar to ingestion.
Tris-Base
Coughing, shortness of breath.
Boric Acid
May be absorbed from the mucous membranes of the respiratory tract, and depending on the amount of
exposure could result in symptoms paralleling ingestion.
INGESTION
Urea
Symptoms may include nausea, vomiting, and diarrhea. May also cause headache, confusion and electrolyte
depletion.
Tris-Base
Symptoms may include nausea, vomiting, and diarrhea. Large oral doses may cause weakness, collapse,
blood clotting, and coma. The estimated lethal dose of Tris Base is 50 grams dry solid.
Boric Acid
Depending on the amount of exposure, ingestion could result in the development of nausea, vomiting,
diarrhea, drowsiness, rash, headache, fall in body temperature, low blood pressure, renal injury, cyanosis,
coma, and death. Adult fatal dose reported at 5 to > 30 grams.
SKIN
Urea
Symptoms include redness, itching, and pain.
Tris-Base
Redness, itching, and pain.
Boric Acid
Symptoms of skin absorption parallel inhalation and ingestion.
EYES
Urea
Redness, itching and pain.
Tris-Base
Redness, itching, and pain.
Boric Acid
Redness, itching and pain.
CARCINOGENICITY
Urea
Not listed as a carcinogen by NTP or IARC.
Tris-Base
Not listed as a carcinogen by NTP or IARC.
Boric Acid
Not listed as a carcinogen by NTP or IARC.
MUTAGENICITY
Urea
No information found.
Tris-Base
No information found.
Boric Acid
No information found.
Page 2 SequaGel Buffer
REPRODUCTIVE TOXICITY
Urea
No information found.
Tris-Base
No information found.
Boric Acid
Studies of dogs and rats have shown that infertility and damage to testes can result from acute or chronic
ingestion of boric acid. Evidence of toxic effects on the human reproductive system is inadequate.
TERATOGENIC EFFECTS
Urea
No information found.
Tris-Base
No information found.
Boric Acid
No information found.
ROUTES OF ENTRY
Urea
Ingestion and inhalation.
Tris-Base
Ingestion.
Boric Acid
Ingestion and inhalation. Not significantly absorbed through the intact skin. Readily absorbed through
damaged or burned skin.
TARGET ORGAN STATEMENT
Urea
Supersensitive individuals with skin or eye problems, kidney impairment or asthmatic condition should have
physician's approval before exposure to urea dust.
Tris-Base
No information available.
Boric Acid
Persons with pre-existing skin disorders or eye problems, or impaired liver, kidney or respiratory function may
be more susceptible to the effects of this substance.
4. FIRST AID MEASURES
INHALATION:
Remove to fresh air. If not breathing, give artificial respiration. If breathing is difficult, give oxygen. Call a
physician.
INGESTION:
Induce vomiting immediately as directed by medical personnel. Never give anything by mouth to an unconscious
person. Call a physician.
SKIN:
Immediately flush skin with plenty of soap and water for at least 15 minutes while removing contaminated clothing
and shoes. Get medical attention. Wash clothing before reuse. Thoroughly clean shoes before reuse.
EYES:
Immediately flush eyes with plenty of water for at least fifteen minutes, lifting lower and upper eyelids occasionally.
Get medical attention immediately.
5. FIRE FIGHTING MEASURES
N.A. N.A.
FLASH POINT: FLAMMABLE LIMITS:
N.A. N.A.
FLASH POINT METHOD: AUTOIGNITION TEMPERATURE:
EXTINGUISHING MEDIA
Use media appropriate to the primary cause of fire.
PROTECTIVE EQUIPMENT
In the event of a fire, wear full protective clothing and NIOSH-approved self-contained breathing apparatus with full
facepiece operated in the pressure demand or other positive pressure mode.
Page 3 SequaGel Buffer
HAZARDOUS COMBUSTION PRODUCTS:
Thermal decomposition products may include toxic oxides of nitrogen and carbon.
UNUSUAL FIRE AND EXPLOSION HAZARDS
Not considered an explosion hazard.
NFPA CODES: Health: 1 Flammability: 0 Reactivity: 0
6. ACCIDENTAL RELEASE MEASURES
STEPS TO BE TAKEN IN CASE MATERIAL IS RELEASED OR SPILLED
Contain and clean up spill immediately, prevent from entering floor drains. Contain liquids using absorbents.
Shovel all spill materials into disposal drum. Scrub spill area with detergent, flush with copious amounts of water.
WASTE DISPOSAL METHOD
Disposal must be made in accordance with applicable federal, state, and local regulations.
PERSONAL PRECAUTIONS
Wear appropriate protective equipment as specified in section 8.
7. HANDLING AND STORAGE
HANDLING
Avoid contact and inhalation. Do not get in eyes, on skin, on clothing. Wash thoroughly after handling.
STORAGE
Keep in a tightly closed container, stored in a cooled, dry, ventilated area.
Room Temperature
STORAGE TEMPERATURE:
DISPOSAL
Observe all national, state, and local regulations regarding disposal.
8. EXPOSURE CONTROLS / PERSONAL PROTECTION
AIRBORNE EXPOSURE LIMITS:
Urea
Component:
10 mg/m3, 8-hour TWA
ACGIH Threshold Limit Value (TLV):
not available
OSHA Permissible Exposure Limit (PEL):
Tris-Base
Component:
none established
ACGIH Threshold Limit Value (TLV):
none established
OSHA Permissible Exposure Limit (PEL):
Boric Acid
Component:
10 mg/m3 total dust
ACGIH Threshold Limit Value (TLV):
15 mg/m3 total dust
OSHA Permissible Exposure Limit (PEL):
ENGINEERING CONTROLS
A system of local and/or general exhaust is recommended to keep employee exposures below the Airborn
Exposure Limits. Local exhaust ventilation is generally preferred because it can control the emissions of the
contaminant at its source.
RESPIRATORY PROTECTION
For conditions of use where exposure to the dust or mist is apparent, a full-face dust/mist respirator may be worn.
For emergencies or instances where the exposure levels are not known, use a full-face positive-pressure, air-
supplied respirator.
EYE PROTECTION
Use chemical safety goggles and/or a full face shield where splashing is possible. Maintain eye wash fountain and
quick-drench facilities in work area.
Page 4 SequaGel Buffer
SKIN PROTECTION
Wear protective gloves and clean body covering clothing.
OTHER CONTROL MEASURES
N.A.
9. PHYSICAL PROPERTIES
220 F 1.0
Boiling Point Evaporation Rate
N.A. Soluble
Melting Point Solubility in Water
Water 8.3
Vapor Pressure pH
mm Hg
N.A. 1.16
Vapor Density Specific Gravity
Air = 1 (H20 = 1)
31
% Volatile by Volume
10. STABILITY AND REACTIVITY
STABILITY
Stable under ordinary conditions of use and storage. If moisture is present, boric acid can be corrosive to iron.
CONDITIONS TO AVOID
Heat, incompatibles.
HAZARDOUS DECOMPOSITION PRODUCTS
Urea decomposes upon heating and can form products including ammonia, oxides of nitrogen, cyanuric acid,
cyanic acid, biuret, and carbon dioxide. Boric acid loses chemically combined water upon heating, forming
metaboric acid (HBO2) at 212 - 221F, then (pyroboric acid (H2B4O7) at 285 - 320F, and Boric anhydride at higher
temperatures. The burning of tris base may produce carbon monoxide, carbon dioxide, and nitrogen oxides.
HAZARDOUS POLYMERIZATION
Will not occur
INCOMPATIBLES
Urea
Urea reacts with calcium hypochlorite or sodium hypochlorite to form the explosive nitrogen trichloride. It is
incompatible with sodium nitrite, gallium perchlorate, strong oxidizing agents (permanganate, dichromate, nitrate,
chlorine), phosphorus penta
Boric Acid
Potassium, acetic anhydride, alkalis, carbonates, and hydroxides.
Tris-Base
No incompatibility data found.
11. TOXICOLOGICAL INFORMATION
PRODUCT LD50 VALUES
SequaGel Buffer 19252
Oral Rat LD50 (mg/kg):
SequaGel Buffer No information found.
Dermal Rabbit LD50 (mg/kg):
COMPONENT CANCER LIST STATUS
NTP Carcinogen
Component Known Anticipated IARC Category
Urea No No None
Tris-Base No No None
Boric Acid No No None
12. ECOLOGICAL INFORMATION
Urea
When urea is released to soil, this material will hydrolyze into ammonium in a matter of days to several weeks. When
released into the soil, this material may leach into groundwater. When released into water, this material may
Page 5 SequaGel Buffer
biodegrade to a moderate extent. When released into water, this material is not expected to evaporate significantly.
This material has an experimentally determined bioconcentration factor (BCF) of less than 100. This material is not
expected to significantly bioaccumulate. When released into the air, this material is expected to be readily degraded by
reaction with photochemically produced hydroxyl radicals. When released into the air, this material is expected to have
a half-life of less than 1 day.
Tris-Base
No information found on either the environmental fate or environmental toxicity of this material.
Boric Acid
The EC50/48-hour values for daphnia with boric acid are over 100 mg/l. This material may be toxic to aquatic life..
13. DISPOSAL CONSIDERATIONS
Observe all national, state, and local regulations regarding disposal.
14. TRANSPORT INFORMATION
D.O.T.
Proper Shipping Name: Not Regulated
Hazard Class: N.A.
UN Number: N.A.
Packing Group: N.A.
I.A.T.A.
Proper Shipping Name: Not Regulated
Hazard Class: N.A.
UN Number: N.A.
Packing Group: N.A.
I.M.O.
Proper Shipping Name: Not Regulated
Hazard Class: N.A.
UN Number: N.A.
Packing Group: N.A.
15. REGULATORY INFORMATION
UNITED STATES
TSCA Regulatory:
All intentional ingredients are listed on the TSCA Inventory.
SARA 311/312 Hazard Categories
Component Fire Pressure Reactivity Acute Chronic
Urea No No No Yes Yes
Tris-Base No No No Yes No
Boric Acid No No No Yes Yes
EUROPE
EEC Regulatory:
All intentional ingredients are listed on the European EINECS Inventory.
16. OTHER INFORMATION
NFPA CODES: Health: 1 Flammability: 0 Reactivity: 0
Page 6 SequaGel Buffer
MANUFACTURER DISCLAIMER: The information given herein is offered in good faith as accurate, but without
guarantee. Conditions of use and suitability of the product for particular uses are beyond our control. All risks of use of
the product are therefore assumed by the user. Nothing is intended as a recommendation for uses which infringe valid
patents or as extending license under valid patents. Appropriate warnings and safe handling procedures should be
provided to handlers and users.
Page 7 SequaGel Buffer
|