Material Safety Data Sheet
HaltTM Phosphatase Inhibitor Cocktail
1. Product and company identification
: HaltTM Phosphatase Inhibitor Cocktail
Product name
: ThermoFisher Scientific : ThermoFisher Scientific
Supplier Manufacturer
Pierce Biotechnology Pierce Biotechnology
P.O. Box 117 P.O. Box 117
Rockford, IL 61105 Rockford, IL 61105
United States United States
815.968.0747 or 815.968.0747
800.874.3723 800.874.3723
78420 78426 78427 78428 1861277 1901694
Product No. :
7358
MSDS # :
Validation date : 12/7/2007.
12/7/2007.
Print date :
Responsible name : MSDS_Specialist
CHEMTREC: Use of : Refer to the instruction booklet for
:
In case of emergency
800.424.9300 Substance/Preparation proper and intended use.
OUTSIDE US: Otherwise, contact supplier for
202.483.7616 specific applications.
2. Hazards identification
: Liquid. [Clear sparkling liquid.]
Physical state
: Odorless.
Odor
: This material is considered hazardous by the OSHA Hazard Communication Standard
OSHA/HCS status
(29 CFR 1910.1200).
: WARNING !
Emergency overview
HARMFUL IF SWALLOWED. CAUSES RESPIRATORY TRACT, EYE AND SKIN
IRRITATION. MAY BE HARMFUL IF ABSORBED THROUGH SKIN. CONTAINS
MATERIAL THAT CAN CAUSE TARGET ORGAN DAMAGE. SUSPECT CANCER
HAZARD - CONTAINS MATERIAL WHICH MAY CAUSE CANCER.
Toxic if swallowed. Harmful in contact with skin. Irritating to eyes, respiratory system
and skin. Avoid exposure - obtain special instructions before use. Do not breathe vapor
or mist. Do not ingest. Do not get in eyes. Avoid contact with skin and clothing.
Contains material that can cause target organ damage. Contains material which may
cause cancer. Risk of cancer depends on duration and level of exposure. Use only with
adequate ventilation. Keep container tightly closed and sealed until ready for use. Wash
thoroughly after handling.
: Eye contact. Inhalation. Ingestion.
Routes of entry
Potential acute health effects
: Irritating to respiratory system.
Inhalation
: Toxic if swallowed.
Ingestion
: Harmful in contact with skin. Irritating to skin.
Skin
: Irritating to eyes.
Eyes
Potential chronic health effects
: Contains material that can cause target organ damage.
Chronic effects
: Contains material which may cause cancer. Risk of cancer depends on duration and
Carcinogenicity
level of exposure.
: No known significant effects or critical hazards.
Mutagenicity
: No known significant effects or critical hazards.
Teratogenicity
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2. Hazards identification
: No known significant effects or critical hazards.
Developmental effects
: No known significant effects or critical hazards.
Fertility effects
: Contains material which causes damage to the following organs: heart, bones, teeth.
Target organs
Contains material which may cause damage to the following organs: kidneys,
gastrointestinal tract, upper respiratory tract, skin, central nervous system (CNS), eye,
lens or cornea.
Over-exposure signs/symptoms
: Adverse symptoms may include the following:
Inhalation
respiratory tract irritation
coughing
: No specific data.
Ingestion
: Adverse symptoms may include the following:
Skin
irritation
redness
: Adverse symptoms may include the following:
Eyes
pain or irritation
watering
redness
: Pre-existing skin and digestive disorders and disorders involving any other target organs
Medical conditions
mentioned in this MSDS as being at risk may be aggravated by over-exposure to this
aggravated by over-
product.
exposure
The preparation is not classified as dangerous according to Directive 1999/45/EC and its amendments.
: Not classified.
Classification
See toxicological information (section 11)
3. Composition/information on ingredients
United States
Name CAS number %
Sodium Pyrophosphate 7758-16-9 1-3
Sodium Fluoride 7681-49-4 1-3
Sodium Orthovanadate 13721-39-6 1-3
Europe
: Preparation
Substance/preparation
Ingredient name CAS % EC number Classification
number
Sodium Pyrophosphate 7758-16-9 1-3 231-835-0 Xn; R22 [1]
Sodium Fluoride 7681-49-4 1-3 231-667-8 T; R25 [1] [2]
Xi; R36/38
R32
Sodium Orthovanadate 13721-39-6 1 - 3 237-287-9 Xn; R22 [1]
Xi;
R36/37/38
There are no ingredients or additional ingredients present which, within the current knowledge of the supplier and
in the concentrations applicable, are classified as hazardous to health or the environment and hence require
reporting in this section.
[1] Substance classified with a health or environmental hazard
[2] Substance with a workplace exposure limit
Occupational exposure limits, if available, are listed in section 8.
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4. First aid measures
: Immediately flush eyes with plenty of water for at least 15 minutes, occasionally lifting
Eye contact
the upper and lower eyelids. Check for and remove any contact lenses. Get medical
attention.
: Get medical attention immediately. Flush contaminated skin with plenty of water.
Skin contact
Remove contaminated clothing and shoes. Wash contaminated clothing thoroughly with
water before removing or wear gloves. Continue to rinse for at least 10 minutes. Wash
clothing before reuse. Clean shoes thoroughly before reuse.
: Get medical attention immediately. Move exposed person to fresh air. If it is suspected
Inhalation
that fumes are still present, the rescuer should wear an appropriate mask or self-
contained breathing apparatus. Keep person warm and at rest. If not breathing, if
breathing is irregular or if respiratory arrest occurs, provide artificial respiration or oxygen
by trained personnel. It may be dangerous to the person providing aid to give mouth-to-
mouth resuscitation. If unconscious, place in recovery position and get medical attention
immediately. Maintain an open airway. Loosen tight clothing such as a collar, tie, belt or
waistband.
: Get medical attention immediately. Wash out mouth with water. Remove dentures if
Ingestion
any. Move exposed person to fresh air. Keep person warm and at rest. If material has
been swallowed and the exposed person is conscious, give small quantities of water to
drink. Stop if the exposed person feels sick as vomiting may be dangerous. Do not
induce vomiting unless directed to do so by medical personnel. If vomiting occurs, the
head should be kept low so that vomit does not enter the lungs. Never give anything by
mouth to an unconscious person. If unconscious, place in recovery position and get
medical attention immediately. Maintain an open airway. Loosen tight clothing such as
a collar, tie, belt or waistband.
: No action shall be taken involving any personal risk or without suitable training. If it is
Protection of first-aiders
suspected that fumes are still present, the rescuer should wear an appropriate mask or
self-contained breathing apparatus. It may be dangerous to the person providing aid to
give mouth-to-mouth resuscitation. Wash contaminated clothing thoroughly with water
before removing or wear gloves.
See section 11 for more detailed information on health effects and symptoms.
5. Fire-fighting measures
: In a fire or if heated, a pressure increase will occur and the container may burst.
Flammability of the product
Extinguishing media
: Use an extinguishing agent suitable for the surrounding fire.
Suitable
: None known.
Not suitable
: Promptly isolate the scene by removing all persons from the vicinity of the incident if
Special exposure hazards
there is a fire. No action shall be taken involving any personal risk or without suitable
training.
: Decomposition products may include the following materials:
Hazardous combustion
carbon oxides
products
phosphorus oxides
halogenated compounds
metal oxide/oxides
: Fire-fighters should wear appropriate protective equipment and self-contained breathing
Special protective
apparatus (SCBA) with a full face-piece operated in positive pressure mode.
equipment for fire-fighters
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6. Accidental release measures
: No action shall be taken involving any personal risk or without suitable training.
Personal precautions
Evacuate surrounding areas. Keep unnecessary and unprotected personnel from
entering. Do not touch or walk through spilled material. Avoid breathing vapor or mist.
Provide adequate ventilation. Wear appropriate respirator when ventilation is
inadequate. Put on appropriate personal protective equipment (see section 8).
: Avoid dispersal of spilled material and runoff and contact with soil, waterways, drains
Environmental precautions
and sewers. Inform the relevant authorities if the product has caused environmental
pollution (sewers, waterways, soil or air).
: Stop leak if without risk. Move containers from spill area. Approach release from
Large spill
upwind. Prevent entry into sewers, water courses, basements or confined areas. Wash
spillages into an effluent treatment plant or proceed as follows. Contain and collect
spillage with non-combustible, absorbent material e.g. sand, earth, vermiculite or
diatomaceous earth and place in container for disposal according to local regulations
(see section 13). Dispose of via a licensed waste disposal contractor. Contaminated
absorbent material may pose the same hazard as the spilled product. Note: see section
1 for emergency contact information and section 13 for waste disposal.
: Stop leak if without risk. Move containers from spill area. Dilute with water and mop up
Small spill
if water-soluble or absorb with an inert dry material and place in an appropriate waste
disposal container. Dispose of via a licensed waste disposal contractor.
7. Handling and storage
: Put on appropriate personal protective equipment (see section 8). Eating, drinking and
Handling
smoking should be prohibited in areas where this material is handled, stored and
processed. Workers should wash hands and face before eating, drinking and smoking.
Do not get in eyes or on skin or clothing. Do not breathe vapor or mist. Do not ingest.
Use only with adequate ventilation. Wear appropriate respirator when ventilation is
inadequate. Keep in the original container or an approved alternative made from a
compatible material, kept tightly closed when not in use. Empty containers retain
product residue and can be hazardous. Do not reuse container.
: Store in accordance with local regulations. Store in original container protected from
Storage
direct sunlight in a dry, cool and well-ventilated area, away from incompatible materials
(see section 10) and food and drink. Keep container tightly closed and sealed until ready
for use. Containers that have been opened must be carefully resealed and kept upright
to prevent leakage. Do not store in unlabeled containers. Use appropriate containment
to avoid environmental contamination.
8. Exposure controls/personal protection
Product name Exposure limits
United States
sodium fluoride ACGIH TLV (United States).
TWA: 2.5 mg/m?8 hour(s).
MSHA Standard Air (United States).
TWA: 2.5 mg/m?8 hour(s).
NIOSH REL (United States).
TWA: 2.5 mg/m?8 hour(s).
OSHA PEL (United States).
TWA: 2.5 mg/m?8 hour(s).
OSHA PEL Z2 (United States, 11/2006).
TWA: 2.5 mg/m?8 hour(s). Form: Dust
ACGIH TLV (United States, 1/2007). Notes: as F
TWA: 2.5 mg/m? (as F) 8 hour(s).
NIOSH REL (United States, 12/2001). Notes: as F
TWA: 2.5 mg/m? (as F) 10 hour(s).
OSHA PEL (United States, 11/2006). Notes: as F
TWA: 2.5 mg/m? (as F) 8 hour(s).
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8. Exposure controls/personal protection
OSHA PEL 1989 (United States, 3/1989). Notes: as F
TWA: 2.5 mg/m? (as F) 8 hour(s).
Europe
sodium fluoride EU OEL (Europe, 5/2006). Notes: Indicative
8 hours: 2.5 mg/m?8 hour(s).
Consult local authorities for acceptable exposure limits.
: If this product contains ingredients with exposure limits, personal, workplace atmosphere
Recommended monitoring
or biological monitoring may be required to determine the effectiveness of the ventilation
procedures
or other control measures and/or the necessity to use respiratory protective equipment.
: Use only with adequate ventilation. If user operations generate dust, fumes, gas, vapor
Engineering measures
or mist, use process enclosures, local exhaust ventilation or other engineering controls
to keep worker exposure to airborne contaminants below any recommended or statutory
limits.
: Wash hands, forearms and face thoroughly after handling chemical products, before
Hygiene measures
eating, smoking and using the lavatory and at the end of the working period. Appropriate
techniques should be used to remove potentially contaminated clothing. Wash
contaminated clothing before reusing. Ensure that eyewash stations and safety showers
are close to the workstation location.
Personal protection
: Use a properly fitted, air-purifying or air-fed respirator complying with an approved
Respiratory
standard if a risk assessment indicates this is necessary. Respirator selection must be
based on known or anticipated exposure levels, the hazards of the product and the safe
working limits of the selected respirator.
: Chemical-resistant, impervious gloves complying with an approved standard should be
Hands
worn at all times when handling chemical products if a risk assessment indicates this is
necessary.
: Safety eyewear complying with an approved standard should be used when a risk
Eyes
assessment indicates this is necessary to avoid exposure to liquid splashes, mists,
gases or dusts.
: Personal protective equipment for the body should be selected based on the task being
Skin
performed and the risks involved and should be approved by a specialist before handling
this product.
: Emissions from ventilation or work process equipment should be checked to ensure they
Environmental exposure
comply with the requirements of environmental protection legislation. In some cases,
controls
fume scrubbers, filters or engineering modifications to the process equipment will be
necessary to reduce emissions to acceptable levels.
9. Physical and chemical properties
Liquid. [Clear sparkling liquid.]
Physical state :
Colorless to light yellow.
Color :
Odorless.
Odor :
7.8 to 8
pH :
Easily soluble in the following materials: cold water and hot water.
Solubility :
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10 . Stability and reactivity
: The product is stable. Under normal conditions of storage and use, hazardous
Stability
polymerization will not occur.
: Avoid exposure - obtain special instructions before use.
Conditions to avoid
: No specific data.
Materials to avoid
: Under normal conditions of storage and use, hazardous decomposition products should
Hazardous decomposition
not be produced.
products
: Will not occur.
Hazardous polymerization
11 . Toxicological information
United States
Acute toxicity
Product/ingredient name Result Species Dose Exposure
sodium fluoride LD50 Dermal Rat 175 mg/kg -
LD50 Rat 22 mg/kg -
Intraperitoneal
LD50 Intravenous Rat 26 mg/kg -
LD50 Oral Mouse 57 mg/kg -
LD50 Oral Rat 52 mg/kg -
LD50 Oral Rabbit 200 mg/kg -
LD50 Oral Rat 31 mg/kg -
LD50 Rat 175 mg/kg -
Subcutaneous
LDLo Dermal Mouse 300 mg/kg -
LDLo Oral Woman 90 mg/kg -
LDLo Oral Human 71 mg/kg -
TDLo Parenteral Rat 35 mg/kg -
TDLo Parenteral Rat 9 mg/kg -
trisodium orthovanadate LD50 Oral Rat 330 mg/kg -
LD50 Oral Rat 330 mg/kg -
LD50 Rat 50 mg/kg -
Subcutaneous
LDLo Oral Rabbit 100 mg/kg -
TDLo Rat 4.6 ug/kg -
Intraperitoneal
TDLo Rat 4.6 mg/kg -
Intraperitoneal
diphosphoric acid, disodium salt LD Dermal Rabbit >300 mg/kg -
LD50 Oral Mouse 2650 mg/kg -
LD50 Oral Rat 1800 mg/kg -
: Not available.
Conclusion/Summary
Chronic toxicity
: Not available.
Conclusion/Summary
Carcinogenicity
: Not available.
Conclusion/Summary
Classification
Product/ingredient name ACGIH IARC EPA NIOSH NTP OSHA
sodium fluoride A4 3 - - Possible -
trisodium orthovanadate - - - None. - None.
Mutagenicity
: Not available.
Conclusion/Summary
Teratogenicity
: Not available.
Conclusion/Summary
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11 . Toxicological information
Reproductive toxicity
Europe
: Not available.
Conclusion/Summary
: No known significant effects or critical hazards.
Chronic effects
: No known significant effects or critical hazards.
Carcinogenicity
: No known significant effects or critical hazards.
Mutagenicity
: No known significant effects or critical hazards.
Teratogenicity
: No known significant effects or critical hazards.
Developmental effects
: No known significant effects or critical hazards.
Fertility effects
12 . Ecological information
: No known significant effects or critical hazards.
Environmental effects
United States
Aquatic ecotoxicity
Test results available upon request.
: Not available.
Conclusion/Summary
: No known significant effects or critical hazards.
Other adverse effects
13 . Disposal considerations
: The generation of waste should be avoided or minimized wherever possible. Dispose of
Waste disposal
surplus and non-recyclable products via a licensed waste disposal contractor. Disposal
of this product, solutions and any by-products should at all times comply with the
requirements of environmental protection and waste disposal legislation and any regional
local authority requirements. Avoid dispersal of spilled material and runoff and contact
with soil, waterways, drains and sewers.
: Within the present knowledge of the supplier, this product is not regarded as
Hazardous waste
hazardous waste, as defined by EU Directive 91/689/EEC.
Disposal should be in accordance with applicable regional, national and local laws and regulations.
Refer to Section 7: HANDLING AND STORAGE and Section 8: EXPOSURE CONTROLS/PERSONAL PROTECTION
for additional handling information and protection of employees.
14 . Transport information
Regulatory UN number Proper shipping Classes PG*
information name
Not - - -
DOT Classification
regulated.
Not available. Not available. Not available. -
IATA-DGR Class
PG* : Packing group
15 . Regulatory information
United States
: Toxic material
HCS Classification
Irritating material
Carcinogen
Target organ effects
: TSCA 8(a) PAIR: trisodium orthovanadate
U.S. Federal regulations
United States inventory (TSCA 8b): All components are listed or exempted.
TSCA precursor chemical list: sodium fluoride
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15 . Regulatory information
SARA 302/304/311/312 extremely hazardous substances: No products were found.
SARA 302/304 emergency planning and notification: No products were found.
SARA 302/304/311/312 hazardous chemicals: trisodium orthovanadate; sodium
fluoride
SARA 311/312 MSDS distribution - chemical inventory - hazard identification:
trisodium orthovanadate: Delayed (chronic) health hazard; sodium fluoride: Immediate
(acute) health hazard, Delayed (chronic) health hazard
Clean Water Act (CWA) 307: No products were found.
Clean Water Act (CWA) 311: sodium fluoride
Clean Air Act (CAA) 112 accidental release prevention: No products were found.
Clean Air Act (CAA) 112 regulated flammable substances: No products were found.
Clean Air Act (CAA) 112 regulated toxic substances: No products were found.
: United States inventory (TSCA 8b): All components are listed or exempted.
United States inventory
(TSCA 8b)
Canada
: Class D-1B: Material causing immediate and serious toxic effects (Toxic).
WHMIS (Canada)
Class D-2A: Material causing other toxic effects (Very toxic).
Class D-2B: Material causing other toxic effects (Toxic).
: CEPA Toxic substances: The following components are listed: Inorganic fluorides
Canadian lists
Canadian ARET: None of the components are listed.
Canadian NPRI: The following components are listed: Sodium fluoride;Vanadium
Alberta Designated Substances: None of the components are listed.
Ontario Designated Substances: None of the components are listed.
Quebec Designated Substances: None of the components are listed.
: Canada inventory: All components are listed or exempted.
Canada inventory
EU regulations
: This product is not classified according to EU legislation.
Risk phrases
:
International regulations
: Australia inventory (AICS): All components are listed or exempted.
International lists
China inventory (IECSC): All components are listed or exempted.
Korea inventory (KECI): All components are listed or exempted.
Philippines inventory (PICCS): Not determined.
Japan inventory (ENCS): Not determined.
16 . Other information
: HARMFUL IF SWALLOWED. CAUSES RESPIRATORY TRACT, EYE AND SKIN
Label requirements
IRRITATION. MAY BE HARMFUL IF ABSORBED THROUGH SKIN. CONTAINS
MATERIAL THAT CAN CAUSE TARGET ORGAN DAMAGE. SUSPECT CANCER
HAZARD - CONTAINS MATERIAL WHICH MAY CAUSE CANCER.
Hazardous Material :
Information System (U.S.A.)
3
Health
0
Flammability
0
Physical hazards
The customer is responsible for determining the PPE code for this material.
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16 . Other information
National Fire Protection :
Association (U.S.A.)
Flammability
0
3 0
Health Instability
Special
Date of printing : 12/7/2007.
: 12/7/2007.
Date of issue
Date of previous issue : No previous validation.
Version : 0.01
Indicates information that has changed from previously issued version.
: R25- Toxic if swallowed.
Full text of R-phrases
R22- Harmful if swallowed.
referred to in sections 2 and
R36/38- Irritating to eyes and skin.
3 - Europe
R36/37/38- Irritating to eyes, respiratory system and skin.
R32- Contact with acids liberates very toxic gas.
: T - Toxic
Full text of classifications
Xn - Harmful
referred to in sections 2 and
Xi - Irritant
3 - Europe
Notice to reader
To the best of our knowledge, the information contained herein is accurate. However, neither the above-named
supplier, nor any of its subsidiaries, assumes any liability whatsoever for the accuracy or completeness of the
information contained herein.
Final determination of suitability of any material is the sole responsibility of the user. All materials may present
unknown hazards and should be used with caution. Although certain hazards are described herein, we cannot
guarantee that these are the only hazards that exist.
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