SibEnzyme Ltd.
Ak. Timakov str., 2/12, Novosibirsk, 630117, Russia Tel.: +7 383 333 49 91
E-mail: info@sibenzyme.com Tel/Fax: +7 383 333 68 53
http://www.sibenzyme.com
MATERIAL SAFETY DATA SHEET
(The format of the MSDS complies with Commission Directive 2001/58/EC as amended.)
Revised: 15 January 2009
1. IDENTIFICATION OF THE PREPARATION/SUBSTANCE AND OF THE COMPANY
Preparation Name:
SE-buffer Y - buffered aqueous solution.
(Supplied as a separate unit or as a component of sets with restriction endonucleases:
Aat II (E287, E288, SE288, ME288, LE288); Acc36 I (E289, E290, SE290, ME290, LE290);
AccBS I (E007, E008, SE008, ME008, LE008); Acl I (E011, E012, SE012, ME012, LE012);
AclW I (E211, E212, SE212, ME212, LE212); Acu I (E451, E452, SE452, ME452, LE452);
Afe I (E213, E214, E214X, SE214, ME214, LE214); Ags I (E573, E574, SE574, ME574, LE574);
Ajn I (E473, E474, SE474, ME474, LE474); Alu I (E015, E016, SE016, ME016, LE016);
Apa I (E019, E020, SE020, ME020, LE020); Ars I (E575, E576, SE576, ME576, LE576);
AsuC2 I (E257, E258, SE258, ME258, LE258); AsuNH I (E063, E064, SE064, ME064, LE064);
Bmu I (E487, E488, SE488, ME488, LE488); Bse1 I (E035, E036, SE036, ME036, LE036);
Bse21 I (E037, E038, SE038, ME038, LE038); BslF I (E479, E480, SE480, ME480, LE480);
BspFN I (E557, E558, SE558, ME558, LE558); BssEC I (E273, E274, SE274, ME274, LE274);
Bst2B I (E043, E044, SE044, ME044, LE044); Bst4C I (E265, E266, SE266, ME266, LE266);
Bst6 I (E239, E240, SE240, ME240, LE240); BstC8 I (E305, E306, SE306, ME306, LE306);
BstDS I (E083, E084, SE084, ME084, LE084); BstEN I (E103, E104, SE104, ME104, LE104);
BstF5 I (E031, E032, SE032, ME032, LE032); BstFN I (E283, E284, SE284, ME284, LE284);
BstH2 I (E171, E172, SE172, ME172, LE172); BstHH I (E143, E144, SE144, ME144, LE144);
BstMW I (E459, E460, SE460, ME460, LE460); BstPA I (E299, E300, SE300, ME300, LE300);
BstSC I (E307, E308, SE308, ME308, LE308); BstV2 I (E297, E298, SE298, ME298, LE298);
CciN I (E203, E204, SE204, ME204, LE204); Dri I (E193, E194, SE194, ME194, LE194);
DseD I (E241, E242, SE242, ME242, LE242); Fae I (E495, E496, SE496, ME496, LE496);
FauND I (E009, E010, SE010, ME010, LE010); Fbl I (E271, E272, SE272, ME272, LE272);
Fok I (E247, E248, SE248, ME248, LE248); FriO I (E157, E158, SE158, ME158, LE158);
Fsp4H I (E095, E096, SE096, ME096, LE096); Hpa I (E077, E078, SE078, ME078, LE078);
HspA I (E069, E070, SE070, ME070, LE070); Mbo II (E471, E472, SE472, ME472, LE472);
MspAI I (E191, E192, SE192, ME192, LE192); PalA I (E483, E484, SE484, ME484, LE484);
Pce I (E105, E106, SE106, ME106, LE106); Ple19 I (E195, E196, SE196, ME196, LE196);
Pps I (E269, E270, SE270, ME270, LE270); PspL I (E223, E224, SE224, ME224, LE224);
PspN4 I (E089, E090, SE090, ME090, LE090); PspOM I (E215, E216, SE216, ME216, LE216);
PspPP I (E255, E256, SE256, ME256, LE256); PspX I (E477, E478, E478X, SE478, ME478, LE478);
Psr I (E131, E132, SE132, ME132, LE132); Rga I (E491, E492, SE492, ME492, LE492);
Rsr2 I (E281, E282, SE282, ME282, LE282); Sbf I (E101, E102, SE102, ME102, LE102);
Set I (E537, E538, E538X, SE538, ME538, LE538); Sma I (E177, E178, SE178, ME178, LE178);
Tag I (E133, E134, SE134, ME134, LE134) Tth111 I (E097, E098, SE098, ME098, LE098);
Xma I (E233, E234, SE234, ME234, LE234); Zrm I (E005, E006, SE006, ME006, LE006)).
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Catalogue number of the product:
B005.
Use of the preparation:
For laboratory use.
Company identification:
SibEnzyme Ltd.
2/12 Timakova Str.
630117 Novosibirsk
Russia
Telephone: +7 383 333 4991
Telephone/Fax: +7 383 333 6853
Site: http://www.sibenzyme.com
E-mail: info@sibenzyme.com
Emergency telephone number:
+7 383 333 6853
2. COMPOSITION/ INFORMATION ON INGREDIENTS
Ingredients:
1. Tris-acetate < 1% (w/v)
(mix of Tris (Trometamol) EC#201-064-4 CAS#77-86-1
and Acetic acid EC#200-580-7 CAS#64-19-7)
2. Magnesium acetate < 1% (w/v) EC#205-554-9 CAS#142-72-3
3. Potassium acetate < 1% (w/v) EC#204-822-2 CAS#127-08-2
4. DTT (Dithiothreitol) ~ 0.1% (w/v) EC#222-468-7 CAS#3483-12-3
5. Water ~ 98% (v/v) EC#231-791-2 CAS#7732-18-5
Ingredients indicated above do not present a health or environmental hazard within the meaning of
Council Directive 67/548/EEC (and Commission Directive 2008/58/EC), or their concentrations do
not exceed the limits specified in Council Directive 1999/45/EC, and in Commission Directive
2001/58/EC.
3. HAZARDS IDENTIFICATION
The preparation is not classified as dangerous according to Directive 1999/45/EC or Directive
67/548/EC, and is not labelled with danger symbols or indications of danger.
Hazards deriving from physical-chemical properties:
The data available do not support any physical or chemical hazard.
Human health hazards:
Inappropriate handling may cause formation of aerosols. Inhalation of enzyme aerosols may
cause allergic type reactions in sensitized individuals. Prolonged skin contact may cause
minor irritation.
Caution: Avoid inhalation, ingestion, and skin contacts.
Environmental hazards:
The data available do not support any environmental hazard.
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4. FIRST-AID MEASURES
Ingestion:
If swallowed, rinse mouth and throat thoroughly with water. Consult a doctor in event of any
complaints.
Inhalation:
If inhaled, remove victim from area of exposure. If symptoms of irritation or respiratory
allergy occur, consult a doctor.
Skin contact:
Generally, the product does not irritate the skin. If symptoms of irritation after prolonged
exposure occur, consult a doctor.
Eye contact:
In case of contact, wash out opened eyes with plenty of water for several minutes. Consult a
doctor in event of any complaints.
5. FIRE-FIGHTING MEASURES
The preparation cannot cause fire.
Suitable extinguishing agents:
Water spray, Carbon Dioxide, dry chemical powder or appropriate foam.
Not suitable extinguishing agents:
None.
Special exposure hazards from decomposition products:
Not thoroughly investigated.
Protective equipment:
Wear self-contained breathing apparatus and protective clothing.
6. ACCIDENTAL RELEASE MEASURES
Personal precautions:
Wear appropriate gloves (see Section 8), safety glasses to clean small releases/spillage. In
case of vapour/aerosol formation, wear suitable respiratory protection means. Ventilate the
area.
Environmental precautions:
No special measures required.
Methods for cleaning up:
Absorb spillages with liquid-binding material. Ventilate and wash the area after the spillage
is completely removed.
Additional information:
No dangerous substances are released.
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7. HANDLING AND STORAGE
Handling:
No further requirements.
Use appropriate personal protection measures (see Section 8). Avoid any contact with the
liquid. Avoid the formation of aerosols. Ventilate area.
Storage:
Store at - 20癈.
None other specific requirements relating to the preparation.
8. EXPOSURE CONTROLS/PERSONAL PROTECTION
Ingredients with limit values that require monitoring at the workplace:
The product does not contain any relevant quantities of materials with critical values that
need to be monitored at the workplace.
General protective and hygienic measures:
The usual precautionary measures are the same as for chemicals handling.
Engineering measures:
None specific requirements relating to the preparation.
Personal protection:
- Respiratory protection: No special measures required.
- Hand protection: Wear rubber gloves. None special requirements to rubber.
- Eye protection: No need to wear glasses (goggles) when ordinary operations with the
preparation. Safety glasses are recommended during certain operations when aerosol
formation is possible.
- Skin protection: The usual precautionary measures accepted in research laboratories, e.g.
protective clothes (gowns). None specific requirements.
9. PHYSICAL AND CHEMICAL PROPERTIES
Appearance: Liquid.
Colour: Colourless.
Odour: Odourless.
pH-value at 25癈: 7.9.
Boiling point: Undetermined.
Melting Point: Undetermined.
Flash Point: Not applicable.
Flammability/autoflammability: The preparation is not flammable/self-igniting.
Explosive properties: The preparation does not possess explosive properties.
Oxidizing properties: Not applicable.
Vapour pressure: Not applicable.
Relative density at 20癈: ~1.1 g/cm3.
Solubility in/miscibility with water: Fully miscible.
Fat solubility: Not applicable.
Vapour density: Not applicable.
Evaporation rate: Not applicable.
Conductivity: Not applicable.
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Viscosity: Not viscous.
10. STABILITY AND REACTIVITY
Stability:
Stable under storage conditions (see Section 7).
Conditions to avoid:
No conditions which may cause dangerous reactions.
Possibility of hazardous reactions:
No dangerous reactions known.
Materials to avoid:
No materials which may cause dangerous reactions.
Hazardous decomposition products:
No dangerous decomposition products known.
Hazardous polymerization:
Will not occur.
11. TOXICOLOGICAL INFORMATION
Routes of exposure:
Inhalation: Inhalation of aerosols may cause allergic type reactions in sensitized
individuals.
Ingestion: Repeated ingestion or excessive dosage may cause irritation of the
gastrointestinal tract.
Skin and eye contact: Generally, the product does not irritate the skin and eyes. Prolonged
skin or eye contact may cause minor irritation.
Additional toxicological information:
When used and handled according to specifications, the product does not have any harmful
effects.
The product is not listed in a priority list as foreseen under Council Regulation (EEC) No 793/93 on
the evaluation and control of the risks of the existing substances.
12. ECOLOGICAL INFORMATION
Ecotoxicity:
The preparation is considered not dangerous to the environment with respect to mobility,
persistence and degradability, bioaccumulation, aquatic toxicity and other data relating to
ecotoxicity.
Persistence and degradability:
The preparation is readily biodegradable.
Bioaccumulative potential:
It is considered that the preparation does not accumulate in biota, and does not pass through
the food chain.
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Other adverse effects:
Not applicable.
13. DISPOSAL CONSIDERATIONS
The disposal of the product does not present a danger.
No special disposal methods required, except to be in accordance with current local environmental
regulations.
14. TRANSPORT INFORMATION
Special precautions:
None known.
Proper shipping name:
Not dangerous goods according to transport regulations.
National transport regulations:
No additional national transport regulations are known to the supplier.
IMDG: Non-hazardous for sea freight.
ADR: Non-hazardous for road transport.
RID: Non-hazardous for rail transport.
ICAO/IATA: Non-hazardous for air transport.
Other information:
The preparation may be stored in ice during the transportation.
15. REGULATORY INFORMATION
US
Labelling according to US Regulations: Caution: Avoid contact and inhalation.
US Regulatory information: SARA LISTED: No.
EU
Labelling according to EU Guidelines: The preparation is not dangerous within the
meaning of Directives 67/548/EC, 1999/45/EC, and 88/379/ECC, and is not labelled with
danger symbols or indications of danger.
Canada
WHMIS classification: This product has been classified in accordance with the hazard
criteria of the CPR, and the MSDS contains all the information required by the CPR.
DSL: No.
NDSL: No.
16. OTHER INFORMATION
The above information is believed to be correct but does not purport to be all-inclusive and shall be
used only as a guide. SibEnzyme shall not be held liable for any damage resulting from handling or
from contact with the above product.
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