MATERIAL SAFETY DATA SHEET
1-866-800-5458
NFORMATION CALLS
Protein
1-978-927-3382
FAX
#H0250
information@biohelix.com
E-MAIL
? February 2006
DATE
SECTION 1 - CHEMICAL INFORMATION
Product Name: T7 Gp4A Helicase
SECTION 2 - CHEMICAL INFORMATION
1. Glycerol 50% Cas. #56-81-5
2. Sodium Choride <1% Cas. #7647-14-5
3. Tris-HCl <1% Cas. #77-86-1
4. EDTA <1% Cas. #60-00-4
5. Dithiothreitol <1% Cas. #3483-12-3
SECTION 3 - COMPOSITION/ INFORMATION ON INGREDIENT
CHEMICAL NAME: GLYCEROL
CAS No.: 56-81-5 MF: C3H803 EC No.: 200-289-5
SYNONYMS: CITIFLOUR AF 2* GLYCERIN* GLYCERIN, ANHYDROUS* GLYCERINE* GLYCERINE MIST (ACGIH, OSHA)* GLYCERIN,
SYNTHETIC* CLYCERITOL GLYCYL ALCOHOL* CLYZERIN, WASSERFREI (GERMAN)* GROCOLENE* OSMOGLYN* 1,2,3-PROPA-
NETRIOL* STAR* SYNTHETIC GLYCERIN* TECHNICAL GLYCERINE* TRIHYDROXYPROPANE* 1,2,3-TRIHYDROXYPROPANE.
SECTION 4 - HADARDOUS IDENTIFICATION
LABEL PRECAUTIONARY STATEMENTS:
CAUTION Target Organ (s):
Avoid contact by inhalation, skin and ingestion. Kidney, Hygroscopic.
SECTION 5 - FIRST AID MEASURES:
EMERGENCY OVERVIEW: OCCUPATOINAL EXPOSURE PRESENTS LITTLE OR NO HEALTH HAZARD.
POTENTIAL HEALTH EFFECTS:
EYE EXPOSURE: In case of contact, immediately flush eyes with copious amounts of water for at least 15 minutes. As-
sure adequate flushing by separating the eyelids with fingers. Call a physician.
INHALATION EXPOSURE: If inhaled, remove to fresh air. If breathing is difficult, call a physician.
DERMAL EXPOSURE: In case of contact, immediately wash skin with soap and copious amounts of water. Remove
clothing and call a physician.
ORAL EXPOSURE: If swallowed, wash out mouth with water provided person is conscious. Call a physician.
SECTION 6 - FIRE FIGHTING MEASURES
Extinguishing Media:
-Water Spray
-Carbon Dioxide, Dry Chemical powder or appropriate foam
Unusual Fire and Explosions Hazard (s):
Emits toxic fumes under fire conditions.
Special Firefighting Procedures:
Wear self-contained breathing apparatus and protective clothing to prevent contact with skin and eyes.
Prevent contact with skin and eyes.
SECTION 7 - ACCIDENTAL RELEASE MEASURES
PROCEDURE(S) OF PERSONAL PRECAUTION(S):
Wear self-contained breathing apparatus, chemical safety goggles, rubber boots, and chemically resistant gloves.
METHODS FOR CLEANING UP:
Absorb on sand or vermiculite and place in a closed container for disposal.
SECTION 8 - HANDLING AND STORAGE
HANDLING: User Exposure: Avoid contact with eyes, skin, and clothing. Avoid inhalation. Avoid prolonged or repeated
exposure.
STORAGE: Suitable: Keep tightly closed. Store at -20癈
SECTION 9 - EXPOSURE CONTROLS/PPE
Engineering Controls: Safety shower and eye bath. Mechanical exhaust required.
Personal Protective Equipment: General Hygiene Measures:
Respiratory: Wash thoroughly after handing.
NIOSH/MSHA-approved respirator. Wash contaminated clothing before use.
Hand: AVOID INHALATION
Compatible chemical-resistant gloves. KEEP TIGHTLY CLOSED.
Eye: STORE IN A COOL DRY PLACE.
Compatible safety goggles. FREEZE.
STORE AT ?0 篊.
SECTION 10 - PHYSICAL AND CHEMICAL PROPERTIES
Physical Properties: Explosion Limits in Air:
Melting Point: 20癈 Lower: 0.9%
Boling Point: 182癈 Vapor Density: 3.1 G/L
Flash Point: >392癋, >200癈 Specific Gravity: 1.262
pH: 5.5-8.0
Solubility: Water -Z26130
Vapor Pressure: < 1 MMHG @ 20癈
SECTION 11 - STABILITY AND REACTIVITY
Stability: Hazardous Decomposition Products:
Stable Carbon Monoxide, Carbon Dioxide.
Materials to Avoid: Hazardous Decomposition Products:
Strong oxidizing agents, strong bases. Will not occur.
SECTION 12 - TOXICOLOGICAL INFORMATION
Route of Exposure:
Skin contact: May cause skin irritation.
Eye Contact: May cause eye irritation.
Multiple Routes: May be harmful by inhalation, ingestion, or skin absorption.
Materials may be irritating to mucous membranes and upper respiratory tract.
RTECS #: MA8050000 Chronic Effects: Target organs, Kidney
GYLCEROL
To the best of our knowledge, the properties have not yet been thoroughly investigated.
IRRITATION DATA:
SKN-RBT 500 MG/24H MLD 85JCAE -, 207, 1986
EYE-RBT 126 MG MLD BIOFX* 9-4/970
EYE-RBT 500 MG/24H MLD 85JCAE -, 207, 1986
TOXICITY DATA:
ORL-RAT LD50: 12600 MG/KG FEPRA7 4, 142, 1945
IHL-RAT LC50: <570 MG/M3/1H BIOFX* 9-4/970
IPR-RAT LD50: 4420 MG/KG RCOCB8 56, 125, 1987
SCU-RAT LD50: 100 MG/KG NIIRDN 6, 215, 1982
IVN-RAT LD50: 5566 MG/KG ARZNAD 26, 1581, 1976
ORL-MUS LD50: 4090 MG/KG FRZKAP (6), 56, 1977
IPR-MUS LD50: 8700 MG/KG ARZNAD 28, 1579, 1978
SCU-MUS LD50: 91 MG/KG NIIRDN 6, 215, 1982
IVN-MUS LD50: 4250 MG/KG JAPMA8 39, 583, 1950
ORL-RBT LD50: 27 MG/KG DMDJAP 31, 276, 1959
SKN-RBT LD50: >10 MG/KG BIOFX* 9-4/970
IVN-RBT LD50: 53 MG/KG NIIRDN 6, 215, 1982
ORL-GPG LD50: 7750 MG/KG JIHTAB 23, 259, 1941
TARGET ORGAN DATA:
Behavioral (headache) Paternal effects (testes, epididymis, sperm duct)
Gastrointestinal (nausea or vomiting) Effects on fertility (male fertility index)
Kidney, ureter, bladder (changes in tubules) Effects on fertility (post-implantation mortality)
Kidney, ureter, bladder (changes in urine composition)
Paternal effects (spermatogenesis)
Only selected registry of toxic effects of chemical substance (RTECS) data is presented here. See actual entry in RTECS.
SECTION 13 - ECOLOGICAL INFORMATION
Data not yet available.
SECTION 14 - DISPOSAL CONSIDERATIONS
Contact a licensed professional waste disposal service to dispose of this material.
Observe all federal, state and local environmental regulations.
Dissolve or mix the material with a combustible solvent and burn in a chemical incinerator equipped with an afterburner
and scrubber.
SECTION 15 - TRANSPORT INFORMATION
Contact BioHelix?Corporation for transport information.
SECTION 16 - REGULATORY INFORMATION
Reviews, standards and regulations:
OEL=MAK
ACGIH TLV-TWA 10 MG/M3 DTLVS* TLV/BEI, 1999
EPA FIFRA 1988 PESTICIDE SUBJECT TO REGISTRATION OR RE-REGISTER FERAC 54, 7740, 1989.
MSHA STANDARD: NUISANCE PARTICULATES (MIST)
DTLWS* 3,20, 1973
OSHA PEL (GEN INDU): 8H TWA 15 MG/M3, TOTAL DUST
CFRGBR 29, 1910. 1000, 1994
OSHA PEL (GEN INDU): 8H TWA 5 MG/M3, RESPIRABLE FRACTION
CFRGBR 29, 1910. 1000, 1994
OSHA PEL (CONSTRUC): 8H TWA 15 MG/M3, TOTAL DUST
CFRGBR 29, 1926.55, 1994
OSHA PEL (CONSTRUC): 8H TWA 5 MG/M3, RESPIRABLE FRACTION
CFRGBR 29, 1926.55, 1994
OSHA PEL (SHIPYARD): 8H TWA 15MG/M3, TOTAL DUST
CFRGBR 29, 1915. 1000, 1993
OSHA PEL (SHIPYARD): 8H TWA 5 MG/M3, RESPIRABLE FRACTION
CFRGBR 29, 1915.1000, 1993
OEL 瑼USTRALIA: TWA 10 MG/M3, JAN 1993
OEL 瑽ELGIUM: TWA 10 MG/M3, JAN 1993
OEL 璅INLAND: TWA 20 MG/M3, JAN 1999
OEL 璅RANCE: VME 10 MG/M3, JAN 1999
OEL - THE NETHERLANDS: MAC-TGG 10 MG/M3, JAN 1999
OEL 璘NITED KINGDOM: TWA 10 MG/M3, MIST SEPT 2000
OEL 瑼RGENTINA, BULGARIA, COLUMBIA, JAPAN, KOREA CHECK ACC
NOHS 1974: HZD 35085; NIS 358; TNF 86657; NOS 198; TNE 1085329
NOHS 1983: HZD 35085; NIS 310; TNF 67054; NOS 215; TNE 2135546; TFE 1346631
EPA TSCA SECTION 8 (B) CHEMICAL INVENTORY
EPA TSCA SECTION 8 (D) UNPUBLISHED HEALTH/SAFETY STUDIES
EPA TSCA TEST SUBMISSION (TSCATS) DATA BASE, JAN 2001
SECTION 17 - OTHER INFORMATION
The above information is believed to be correct but does BioHelix?Corporation shall not be held liable for any
not purport to be all-inclusive and shall be used only as damage resulting from handlin or from contact with the
a guide. above product.
MATERIAL SAFETY DATA SHEET
1-866-800-5458
NFORMATION CALLS
Protein
1-978-927-3382
FAX
#H0250
information@biohelix.com
E-MAIL
? April 2007
DATE
SECTION 1 - PRODUCT
Product Name: 10X Gp4A Buffer
SECTION 2 - CHEMICAL INFORMATION
1. Tris-Acetate <1% Cas. #none
2. Magnesium Acetate <1% Cas. #142-72-3
3. Dithiothreitol <1% Cas. #3483-12-3
4. Triton X-100 <1% Cas. #9002-93-1
SECTION 3 - HADARDOUS IDENTIFICATION
Many Bioheix products are classified as mixtures which contain hazardous ingredients in concentrations (less than 1%). The
quantity of hazardous chemical component does not exceed the permissible exposure limit or "Threshold Limit Value" .
MSDS' are offered by Biohelix since The Hazards Communication Standard set by OSHA does not exempt these mixtures
from disclosure.
SECTION 15 - REGULATORY INFORMATION
United States Regulatory Information: Canada Regulatory Information:
Sara Listed: No WHMIS Classification:
This product has been classification in accordance with the
hazard criteria of the CPR and the MSDS contains all the
information required by the CPR
DSL: No
NDSL: No
SECTION 16 - OTHER INFORMATION
The above information is believed to be correct but does BioHelix Corporation shall not be held liable for any
not purport to be all-inclusive and shall be used only as damage resulting from handlin or from contact with the
a guide. above product.
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