SIGMA-ALDRICH
MATERIAL SAFETY DATA SHEET
Date Printed: 03/03/2004
Date Updated: 09/01/2002
Version 1.4
Section 1 - Product and Company Information
Product Name CRYSTAL VIOLET ACS REAGENT
Product Number C6158
Brand SIGMA
Company Sigma-Aldrich
Street Address 3050 Spruce Street
City, State, Zip, Country SAINT LOUIS MO 63103 US
Technical Phone: 314 771 5765
Emergency Phone: 414 273 3850 Ext. 5996
Fax: 800 325 5052
Section 2 - Composition/Information on Ingredient
Substance Name CAS # SARA 313
CRYSTAL VIOLET 548-62-9 Yes
Ingredient Name CAS # Percent SARA 313
CRYSTAL VIOLET 548-62-9 90 No
ZINC FOIL 7440-66-6 1 No
MICHLER'S KETONE 90-94-8 0.2 Yes
MINERAL OIL 8042-47-5 3 No
Formula C25H30ClN3
Synonyms Adergon * Aizen Crystal Violet * Aizen Crystal
Violet Extra Pure * Aniline Violet * Aniline
Violet Pyoktanine * Atmonil * Avermin * Axuris *
Badil * Basic Violet 3 * Basic Violet BN *
Bismuth Violet * Blaues pyoktanin (German) *
Brilliant Violet 5B * Calcozine Violet C *
Calcozine Violet 6BN * C.I. 42555 * C.I. Basic
Violet 3 * Crystal Violet * Crystal Violet 6B *
Crystal Violet O * Crystal Violet 5BO * Crystal
Violet 6BO * Crystal Violet 10B * Crystal Violet
AO * Crystal Violet AON * Crystal Violet BP *
Crystal Violet BPC * Crystal Violet Chloride *
Crystal Violet Extra Pure * Crystal Violet Extra
Pure APN * Crystal Violet Extra Pure APNX *
Crystal Violet FN * Crystal Violet HL2 * Crystal
Violet Pure DSC * Crystal Violet Pure DSC
Brilliant * Crystal Violet SS * Crystal Violet
Technical * Crystal Violet USP * Gentersal *
Gentian Violet * Gentianaviolett (German) *
Gentiaverm * Genticid * Gentioletten * Hectograph
Violet SR * Hecto Violet R *
Hexamethyl-p-rosaniline chloride * Hexamethy
RTECS Number: BO9000000
Section 3 - Hazards Identification
EMERGENCY OVERVIEW
Toxic. Dangerous for the environment.
May cause cancer. May cause heritable genetic damage. Harmful if
swallowed. Irritating to respiratory system and skin. Risk of
serious damage to eyes. Very toxic to aquatic organisms, may cause
long-term adverse effects in the aquatic environment.
HMIS RATING
HEALTH: 2*
FLAMMABILITY: 0
REACTIVITY: 1
NFPA RATING
HEALTH: 2
FLAMMABILITY: 0
REACTIVITY: 1
*additional chronic hazards present.
For additional information on toxicity, please refer to Section 11.
Section 4 - First Aid Measures
ORAL EXPOSURE
If swallowed, wash out mouth with water provided person is
conscious. Call a physician.
INHALATION EXPOSURE
If inhaled, remove to fresh air. If breathing becomes difficult,
call a physician.
DERMAL EXPOSURE
In case of contact, immediately wash skin with soap and copious
amounts of water.
EYE EXPOSURE
In case of contact with eyes, flush with copious amounts of
water for at least 15 minutes. Assure adequate flushing by
separating the eyelids with fingers. Call a physician.
Section 5 - Fire Fighting Measures
FLASH POINT
N/A
AUTOIGNITION TEMP
N/A
FLAMMABILITY
N/A
EXTINGUISHING MEDIA
Suitable: Water spray. Carbon dioxide, dry chemical powder, or
appropriate foam.
FIREFIGHTING
Protective Equipment: Wear self-contained breathing apparatus
and protective clothing to prevent contact with skin and eyes.
Specific Hazard(s): Emits toxic fumes under fire conditions.
Section 6 - Accidental Release Measures
PROCEDURE TO BE FOLLOWED IN CASE OF LEAK OR SPILL
Evacuate area.
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PROCEDURE(S) OF PERSONAL PRECAUTION(S)
Wear self-contained breathing apparatus, rubber boots, and heavy
rubber gloves. Wear disposable coveralls and discard them after
use.
METHODS FOR CLEANING UP
Sweep up, place in a bag and hold for waste disposal. Avoid
raising dust. Ventilate area and wash spill site after material
pickup is complete.
Section 7 - Handling and Storage
HANDLING
User Exposure: Do not breathe dust. Do not get in eyes, on skin,
on clothing. Avoid prolonged or repeated exposure.
STORAGE
Suitable: Keep tightly closed. Store in a cool dry place.
SPECIAL REQUIREMENTS
Light sensitive.
Section 8 - Exposure Controls / PPE
ENGINEERING CONTROLS
Use only in a chemical fume hood. Safety shower and eye bath.
PERSONAL PROTECTIVE EQUIPMENT
Respiratory: Government approved respirator in nonventilated areas
and/or for exposure above the TLV or PEL.
Hand: Compatible chemical-resistant gloves.
Eye: Chemical safety goggles.
GENERAL HYGIENE MEASURES
Wash contaminated clothing before reuse. Wash thoroughly after
handling.
Section 9 - Physical/Chemical Properties
Appearance Color: Light blue
Form: Fine crystals
Property Value At Temperature or Pressure
Molecular Weight 407.99 AMU
pH N/A
BP/BP Range N/A
MP/MP Range 173 掳C
Freezing Point N/A
Vapor Pressure N/A
Vapor Density N/A
Saturated Vapor Conc. N/A
Bulk Density N/A
Odor Threshold N/A
Volatile% N/A
VOC Content N/A
Water Content N/A
Solvent Content N/A
Evaporation Rate N/A
Viscosity N/A
Surface Tension N/A
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Partition Coefficient N/A
Decomposition Temp. N/A
Flash Point N/A
Explosion Limits N/A
Flammability N/A
Autoignition Temp N/A
Refractive Index N/A
Optical Rotation N/A
Miscellaneous Data N/A
Solubility Solvent: 5 mg/ml EtOH Clear
N/A = not available
Section 10 - Stability and Reactivity
STABILITY
Stable: Stable.
Materials to Avoid: Strong oxidizing agents, Strong reducing
agents.
HAZARDOUS DECOMPOSITION PRODUCTS
Hazardous Decomposition Products: Carbon monoxide, Carbon dioxide,
Nitrogen oxides.
HAZARDOUS POLYMERIZATION
Hazardous Polymerization: Will not occur
Section 11 - Toxicological Information
ROUTE OF EXPOSURE
Skin Contact: Causes skin irritation.
Skin Absorption: May be harmful if absorbed through the skin.
Eye Contact: Causes severe eye irritation.
Inhalation: May be harmful if inhaled. Material is irritating to
mucous membranes and upper respiratory tract.
Ingestion: Harmful if swallowed.
SIGNS AND SYMPTOMS OF EXPOSURE
Prolonged exposure can cause: Nausea, headache, and vomiting.
TOXICITY DATA
Oral
Rat
420 mg/kg
LD50
Intraperitoneal
Rat
8900 UG/KG
LD50
Oral
Mouse
96 mg/kg
LD50
Intraperitoneal
Mouse
5100 UG/KG
LD50
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Oral
Rabbit
150 mg/kg
LD50
Intraperitoneal
Rabbit
5 MG/KG
LD50
Intraduodenal
Rabbit
160 MG/KG
LD50
IRRITATION DATA
Skin
Human
3 mg
3D
I
Remarks: Mild irritation effect
Skin
Human
2 mg
2D
I
Remarks: Mild irritation effect
Skin
Guinea pig
6 mg
3D
I
CHRONIC EXPOSURE - CARCINOGEN
Result: This product is or contains a component that has been
reported to be probably carcinogenic based on its IARC, OSHA,
ACGIH, NTP, or EPA classification.
Species: Rat
Route of Application: Oral
Dose: 10950 MG/KG
Exposure Time: 2Y
Frequency: C
Result: Tumorigenic:Carcinogenic by RTECS criteria.
Endocrine:Thyroid tumors.
Species: Mouse
Route of Application: Oral
Dose: 25750 MG/KG
Exposure Time: 2Y
Frequency: C
Result: Tumorigenic:Carcinogenic by RTECS criteria. Kidney,
Ureter, Bladder:Tumors. Tumorigenic Effects: 0varian tumors.
CHRONIC EXPOSURE - TERATOGEN
Species: Rat
Dose: 100 MG/KG
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Route of Application: Oral
Exposure Time: (6-15D PREG)
Result: Specific Developmental Abnormalities: Musculoskeletal
system. Specific Developmental Abnormalities: Urogenital system.
Species: Rat
Dose: 100 MG/KG
Route of Application: Oral
Exposure Time: (6-15D PREG)
Result: Specific Developmental Abnormalities: Other
developmental abnormalities.
Species: Rabbit
Dose: 7 MG/KG
Route of Application: Oral
Exposure Time: (6-19D PREG)
Result: Effects on Embryo or Fetus: Fetotoxicity (except death,
e.g., stunted fetus).
CHRONIC EXPOSURE - MUTAGEN
Result: May alter genetic material.
Species: Human
Dose: 10 UMOL/L
Cell Type: HeLa cell
Mutation test: DNA inhibition
Species: Human
Dose: 500 UG/L
Cell Type: HeLa cell
Mutation test: Cytogenetic analysis
Species: Human
Dose: 500 UG/L
Cell Type: lymphocyte
Mutation test: Cytogenetic analysis
Species: Rat
Dose: 10 NMOL/L
Cell Type: liver
Mutation test: DNA inhibition
Species: Mouse
Dose: 1 MG/KG
Cell Type: lymphocyte
Mutation test: DNA damage
Species: Hamster
Dose: 500 UG/L
Cell Type: ovary
Mutation test: Cytogenetic analysis
Species: Mammal
Dose: 10 PPH
Cell Type: lymphocyte
Mutation test: DNA
Species: Mammal
Dose: 500 UG/L
Cell Type: Other cell types
Mutation test: Cytogenetic analysis
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Species: Non-mammalian
Dose: 500 UG/L
Cell Type: Other cell types
Mutation test: Cytogenetic analysis
Mutation test: Histidine reversion (Ames)
CHRONIC EXPOSURE - REPRODUCTIVE HAZARD
Species: Rat
Dose: 40 MG/KG
Route of Application: Oral
Exposure Time: (6-15D PREG)
Result: Effects on Fertility: Post-implantation mortality (e.g.,
dead and/or resorbed implants per total number of implants).
Species: Rabbit
Dose: 7 MG/KG
Route of Application: Oral
Exposure Time: (6-19D PREG)
Result: Effects on Fertility: Post-implantation mortality (e.g.,
dead and/or resorbed implants per total number of implants).
Effects on Embryo or Fetus: Fetotoxicity (except death, e.g.,
stunted fetus).
Section 12 - Ecological Information
No data available.
Section 13 - Disposal Considerations
APPROPRIATE METHOD OF DISPOSAL OF SUBSTANCE OR PREPARATION
Contact a licensed professional waste disposal service to dispose
of this material. Observe all federal, state, and local
environmental regulations.
Section 14 - Transport Information
DOT
Proper Shipping Name: Environmentally hazardous
substances, solid, n.o.s.
UN#: 3077
Class: 9
Packing Group: Packing Group III
Hazard Label: Class 9
PIH: Not PIH
IATA
Proper Shipping Name: Environmentally hazardous
substance, solid, n.o.s
IATA UN Number: 3077
Hazard Class: 9
Packing Group: III
Section 15 - Regulatory Information
EU ADDITIONAL CLASSIFICATION
Symbol of Danger: T N
Indication of Danger: Toxic. Dangerous for the environment.
R: 45 46 22 41 50/53
Risk Statements: May cause cancer. May cause heritable genetic
damage. Harmful if swallowed. Risk of serious damage to eyes.
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Very toxic to aquatic organisms, may cause long-term adverse
effects in the aquatic environment.
S: 45 26 36/37/39 22
Safety Statements: In case of accident or if you feel unwell,
seek medical advice immediately (show the label where possible).
In case of contact with eyes, rinse immediately with plenty of
water and seek medical advice. Wear suitable protective
clothing, gloves, and eye/face protection. Do not breathe dust.
US CLASSIFICATION AND LABEL TEXT
Indication of Danger: Toxic. Dangerous for the environment.
Risk Statements: May cause cancer. May cause heritable genetic
damage. Harmful if swallowed. Irritating to respiratory system
and skin. Risk of serious damage to eyes. Very toxic to aquatic
organisms, may cause long-term adverse effects in the aquatic
environment.
Safety Statements: Avoid exposure - obtain special instructions
before use. Do not breathe dust. In case of contact with eyes,
rinse immediately with plenty of water and seek medical advice.
Wear suitable protective clothing, gloves, and eye/face
protection. In case of accident or if you feel unwell, seek
medical advice immediately (show the label where possible).
UNITED STATES REGULATORY INFORMATION
SARA LISTED: Yes
NOTES: This product is or contains a component that is subject
to SARA313 reporting requirements.
TSCA INVENTORY ITEM: Yes
UNITED STATES - STATE REGULATORY INFORMATION
CALIFORNIA PROP - 65
California Prop - 65: This product is or contains chemical(s)
known to the state of California to cause cancer.
CANADA REGULATORY INFORMATION
WHMIS Classification: This product has been classified in
accordance with the hazard criteria of the CPR, and the MSDS
contains all the information required by the CPR.
DSL: Yes
NDSL: No
Section 16 - Other Information
DISCLAIMER
For R&D use only. Not for drug, household or other uses.
WARRANTY
The above information is believed to be correct but does not
purport to be all inclusive and shall be used only as a guide. The
information in this document is based on the present state of our
knowledge and is applicable to the product with regard to
appropriate safety precautions. It does not represent any
guarantee of the properties of the product. Sigma-Aldrich Inc.,
shall not be held liable for any damage resulting from handling or
from contact with the above product. See reverse side of invoice
or packing slip for additional terms and conditions of sale.
Copyright 2004 Sigma-Aldrich Co. License granted to make unlimited
paper copies for internal use only.
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