SIGMA-ALDRICH
MATERIAL SAFETY DATA SHEET
Date Printed: 03/15/2007
Date Updated: 02/05/2006
Version 1.6
Section 1 - Product and Company Information
Product Name TEMED ELECTROPHORESIS REAGENT
Product Number T9281
Brand SIGMA
Company Sigma-Aldrich
Address 3050 Spruce Street
SAINT LOUIS MO 63103 US
Technical Phone: 800-325-5832
Fax: 800-325-5052
Emergency Phone: 314-776-6555
Section 2 - Composition/Information on Ingredient
Substance Name CAS # SARA 313
N,N,N',N'-TETRAMETHYLETHYLENEDIAMIN 110-18-9 No
E
Formula C6H16N2
Synonyms 1,2-Bis-(dimethylamino)ethane *
1,2-Ethanediamine, N,N,N',N'-tetramethyl- (9CI) *
Propamine D * Temed * Tetrameen *
N,N,N',N'-Tetramethyl-1,2-diaminoethane *
N,N,N',N'-Tetramethylethanediamine *
N,N,N',N'-Tetramethyl-1,2-ethanediamine *
Tetramethyl ethylene diamine *
N,N,N',N'-Tetramethylethylenediamine * TMEDA
RTECS Number: KV7175000
Section 3 - Hazards Identification
EMERGENCY OVERVIEW
Flammable (USA) Highly Flammable (EU). Corrosive.
Harmful by inhalation and if swallowed. Causes burns.
HMIS RATING
HEALTH: 3
FLAMMABILITY: 3
REACTIVITY: 0
NFPA RATING
HEALTH: 3
FLAMMABILITY: 3
REACTIVITY: 0
For additional information on toxicity, please refer to Section 11.
Section 4 - First Aid Measures
ORAL EXPOSURE
If swallowed, wash out mouth with water provided person is
conscious. Call a physician immediately. Do not induce vomiting.
INHALATION EXPOSURE
If inhaled, remove to fresh air. If not breathing give
artificial respiration. If breathing is difficult, give oxygen.
DERMAL EXPOSURE
In case of skin contact, flush with copious amounts of water for
at least 15 minutes. Remove contaminated clothing and shoes.
Call a physician.
EYE EXPOSURE
In case of contact with eyes, flush with copious amounts of
water for at least 15 minutes. Assure adequate flushing by
separating the eyelids with fingers. Call a physician.
Section 5 - Fire Fighting Measures
FLAMMABLE HAZARDS
Flammable Hazards: Yes
EXPLOSION HAZARDS
Vapor may travel considerable distance to source of ignition and
flash back. Container explosion may occur under fire conditions.
FLASH POINT
68 掳F 20 掳C Method: closed cup
EXPLOSION LIMITS
Lower: 0.98 % Upper: 9.08 %
AUTOIGNITION TEMP
N/A
FLAMMABILITY
N/A
EXTINGUISHING MEDIA
Suitable: For small (incipient) fires, use media such as
"alcohol" foam, dry chemical, or carbon dioxide. For large
fires, apply water from as far as possible. Use very large
quantities (flooding) of water applied as a mist or spray; solid
streams of water may be ineffective. Cool all affected
containers with flooding quantities of water.
FIREFIGHTING
Protective Equipment: Wear self-contained breathing apparatus
and protective clothing to prevent contact with skin and eyes.
Specific Hazard(s): Flammable liquid. Emits toxic fumes under
fire conditions.
Section 6 - Accidental Release Measures
PROCEDURE TO BE FOLLOWED IN CASE OF LEAK OR SPILL
Evacuate area. Shut off all sources of ignition. Use nonsparking
tools.
PROCEDURE(S) OF PERSONAL PRECAUTION(S)
Wear self-contained breathing apparatus, rubber boots, and heavy
rubber gloves.
METHODS FOR CLEANING UP
Cover with dry-lime, sand, or soda ash. Place in covered
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containers using non-sparking tools and transport outdoors.
Ventilate area and wash spill site after material pickup is
complete.
Section 7 - Handling and Storage
HANDLING
User Exposure: Do not breathe vapor. Do not get in eyes, on
skin, on clothing. Avoid prolonged or repeated exposure.
STORAGE
Suitable: Keep tightly closed. Keep away from heat, sparks, and
open flame.
SPECIAL REQUIREMENTS
Handle and store under inert gas. Air and moisture sensitive.
Section 8 - Exposure Controls / PPE
ENGINEERING CONTROLS
Safety shower and eye bath. Use nonsparking tools. Use only in a
chemical fume hood.
PERSONAL PROTECTIVE EQUIPMENT
Respiratory: Use respirators and components tested and approved
under appropriate government standards such as NIOSH (US) or CEN
(EU). Where risk assessment shows air-purifying respirators are
appropriate use a full-face respirator with multi-purpose
combination (US) or type ABEK (EN 14387) respirator cartridges as
a backup to engineering controls. If the respirator is the sole
means of protection, use a full-face supplied air respirator.
Hand: Compatible chemical-resistant gloves.
Eye: Chemical safety goggles.
Other: Faceshield (8-inch minimum).
GENERAL HYGIENE MEASURES
Wash contaminated clothing before reuse. Discard contaminated
shoes. Wash thoroughly after handling.
Section 9 - Physical/Chemical Properties
Appearance Physical State: Clear liquid
Color: Colorless
Property Value At Temperature or Pressure
Molecular Weight 116.21 AMU
pH N/A
BP/BP Range 120.0 - 122.0 掳C 760 mmHg
MP/MP Range - 55.0 掳C
Freezing Point N/A
Vapor Pressure N/A
Vapor Density N/A
Saturated Vapor Conc. N/A
SG/Density 0.775 g/cm3
Bulk Density N/A
Odor Threshold N/A
Volatile% N/A
VOC Content N/A
Water Content N/A
Solvent Content N/A
Evaporation Rate N/A
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Viscosity N/A
Surface Tension N/A
Partition Coefficient Log Kow: 0.3
Decomposition Temp. N/A
Flash Point 68 掳F 20 掳C Method: closed cup
Explosion Limits Lower: 0.98 %
Upper: 9.08 %
Flammability N/A
Autoignition Temp N/A
Refractive Index 1.4179
Optical Rotation N/A
Miscellaneous Data N/A
Solubility Solubility in Water:10 mg/ml H2O, 20掳C
complete, colorless
Other Solvents: MOSTORGANIC SOLVENTS
N/A = not available
Section 10 - Stability and Reactivity
STABILITY
Stable: Stable.
Conditions of Instability: May discolor on exposure to air and
moisture.
Materials to Avoid: Strong oxidizing agents Carbon dioxide.
Copper, Copper alloys
HAZARDOUS DECOMPOSITION PRODUCTS
Hazardous Decomposition Products: Carbon monoxide, carbon dioxide,
and nitrogen oxides.
HAZARDOUS POLYMERIZATION
Hazardous Polymerization: Will not occur
Section 11 - Toxicological Information
ROUTE OF EXPOSURE
Skin Contact: Causes burns.
Skin Absorption: May be harmful if absorbed through the skin.
Eye Contact: Causes burns.
Inhalation: Material is extremely destructive to the tissue of
the mucous membranes and upper respiratory tract. May be harmful
if inhaled. Harmful if inhaled.
Ingestion: Harmful if swallowed.
SIGNS AND SYMPTOMS OF EXPOSURE
Material is extremely destructive to tissue of the mucous
membranes and upper respiratory tract, eyes, and skin.
Inhalation may result in spasm, inflammation and edema of the
larynxand bronchi, chemical pneumonitis, and pulmonary edema.
TOXICITY DATA
Oral
Rat
268 mg/kg
LD50
Remarks: Brain and Coverings:Other degenerative changes. Spinal
Cord:Other degenerative changes.
Inhalation
Rat
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1,318 ppm
LC50
Remarks: Sense Organs and Special Senses (Nose, Eye, Ear, and
Taste):Eye:Other. Behavioral:Ataxia. Behavioral:Irritability.
Oral
Mouse
630 mg/kg
LD50
Skin
Rabbit
5390 mg/kg
LD50
Subcutaneous
Rabbit
1230 MG/KG
LD50
Oral
Quail
> 316 mg/kg
LD50
IRRITATION DATA
Skin
Rabbit
10 mg
24H
Remarks: Open irritation test
Eyes
Rabbit
0.75 mg
Remarks: Severe irritation effect
Section 12 - Ecological Information
No data available.
Section 13 - Disposal Considerations
APPROPRIATE METHOD OF DISPOSAL OF SUBSTANCE OR PREPARATION
Contact a licensed professional waste disposal service to dispose
of this material. Burn in a chemical incinerator equipped with an
afterburner and scrubber but exert extra care in igniting as this
material is highly flammable. Observe all federal, state, and
local environmental regulations.
Section 14 - Transport Information
DOT
Proper Shipping Name: Flammable liquid, toxic,
corrosive, n.o.s.
UN#: 3286
Class: 3
Packing Group: Packing Group II
Hazard Label: Flammable liquid
Hazard Label: Toxic substances.
Hazard Label: Corrosive
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PIH: Not PIH
IATA
Proper Shipping Name: 1,2-DI-(DIMETHYLAMINO) ETHANE
IATA UN Number: 2372
Hazard Class: 3
Packing Group: II
Section 15 - Regulatory Information
EU DIRECTIVES CLASSIFICATION
Symbol of Danger: F-C
Indication of Danger: Highly Flammable. Corrosive.
R: 11-20/22-34
Risk Statements: Highly flammable. Harmful by inhalation and if
swallowed. Causes burns.
S: 16-26-36/37/39-45
Safety Statements: Keep away from sources of ignition - no
smoking. In case of contact with eyes, rinse immediately with
plenty of water and seek medical advice. Wear suitable
protective clothing, gloves, and eye/face protection. In case of
accident or if you feel unwell, seek medical advice immediately
(show the label where possible).
US CLASSIFICATION AND LABEL TEXT
Indication of Danger: Flammable (USA) Highly Flammable (EU).
Corrosive.
Risk Statements: Harmful by inhalation and if swallowed. Causes
burns.
Safety Statements: Keep away from sources of ignition - no
smoking. In case of contact with eyes, rinse immediately with
plenty of water and seek medical advice. Wear suitable
protective clothing, gloves, and eye/face protection. In case of
accident or if you feel unwell, seek medical advice immediately
(show the label where possible).
UNITED STATES REGULATORY INFORMATION
SARA LISTED: No
TSCA INVENTORY ITEM: Yes
CANADA REGULATORY INFORMATION
WHMIS Classification: This product has been classified in
accordance with the hazard criteria of the CPR, and the MSDS
contains all the information required by the CPR.
DSL: Yes
NDSL: No
Section 16 - Other Information
DISCLAIMER
For R&D use only. Not for drug, household or other uses.
WARRANTY
The above information is believed to be correct but does not
purport to be all inclusive and shall be used only as a guide. The
information in this document is based on the present state of our
knowledge and is applicable to the product with regard to
appropriate safety precautions. It does not represent any
guarantee of the properties of the product. Sigma-Aldrich Inc.,
shall not be held liable for any damage resulting from handling or
from contact with the above product. See reverse side of invoice
or packing slip for additional terms and conditions of sale.
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Copyright 2007 Sigma-Aldrich Co. License granted to make unlimited
paper copies for internal use only.
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