SIGMA-ALDRICH
Material Safety Data Sheet
Date Printed: 12/JUL/2005
Date Updated: 21/DEC/2004
Version 1.2
According to 91/155/EEC
Classified as Hazardous according to the criteria of EU Annex 1 and
NOHSC.
1 - Product and Company Information
Product Name CRYSTAL VIOLET
Product Number C3886
Company Sigma-Aldrich Pty. Ltd.
12 Anella Avenue
Castle Hill NSW 2154
Australia
Technical Phone # +61 2 9841 0555 (1800 800 097)
Fax +61 2 9841 0500 (1800 800 096)
Emergency Phone # +44 8701906777 (1800 448 465)
2 - Composition/Information on Ingredients
Product Name CAS # EC no Annex I
Index Number
CRYSTAL VIOLET None None None
Ingredient Name Percent CAS # EC no Annex I
Index Number
CRYSTAL VIOLET 90 548-62-9 208-953-6 612-204-00-2
Carc. Cat.3
Symbols: Xn N
R-Phrases: 22 40 41 50/53
Harmful if swallowed. Limited evidence of a carcinogenic effect.
Risk of serious damage to eyes. Very toxic to aquatic organisms,
may cause long-term adverse effects in the aquatic environment.
ZINC FOIL 1 7440-66-6 231-175-3 None
MICHLER'S KETONE 0.2 90-94-8 202-027-5 606-073-00-0
Carc. Cat.2 Muta. Cat.3
Symbols: T
R-Phrases: 45 41 68
May cause cancer. Risk of serious damage to eyes. Possible risk of
irreversible effects.
MINERAL OIL 3 8042-47-5 232-455-8 None
Molecular Weight 407.99 AMU
Synonyms Adergon * Aizen Crystal Violet * Aizen Crystal
Violet Extra Pure * Aniline Violet * Aniline
Violet Pyoktanine * Atmonil * Avermin * Axuris *
Badil * Basic Violet 3 * Basic Violet BN *
Bismuth Violet * Blaues pyoktanin (German) *
Brilliant Violet 5B * Calcozine Violet C *
Calcozine Violet 6BN * C.I. 42555 * C.I. Basic
Violet 3 * Crystal Violet * Crystal Violet 6B *
Crystal Violet O * Crystal Violet 5BO * Crystal
Violet 6BO * Crystal Violet 10B * Crystal Violet
AO * Crystal Violet AON * Crystal Violet BP *
Crystal Violet BPC * Crystal Violet Chloride *
Crystal Violet Extra Pure * Crystal Violet Extra
Pure APN * Crystal Violet Extra Pure APNX *
Crystal Violet FN * Crystal Violet HL2 * Crystal
Violet Pure DSC * Crystal Violet Pure DSC
Brilliant * Crystal Violet SS * Crystal Violet
Technical * Crystal Violet USP * Gentersal *
Gentian Violet * Gentianaviolett (German) *
Gentiaverm * Genticid * Gentioletten *
Hectograph Violet SR * Hecto Violet R *
Hexamethyl-p-rosaniline chloride * Hexamethyl
p-rosaniline hydrochloride * Hexamethyl Violet *
Hidaco Brilliant Crystal Violet * Hidaco Crystal
3 - Hazards Identification
SPECIAL INDICATION OF HAZARDS TO HUMANS AND THE ENVIRONMENT
May cause cancer. Also harmful if swallowed. Risk of serious
damage to eyes. Very toxic to aquatic organisms, may cause
long-term adverse effects in the aquatic environment.
4 - First Aid Measures
AFTER INHALATION
If inhaled, remove to fresh air. If breathing becomes difficult,
call a physician.
AFTER SKIN CONTACT
In case of contact, immediately wash skin with soap and copious
amounts of water.
AFTER EYE CONTACT
In case of contact with eyes, flush with copious amounts of
water for at least 15 minutes. Assure adequate flushing by
separating the eyelids with fingers. Call a physician.
AFTER INGESTION
If swallowed, wash out mouth with water provided person is
conscious. Call a physician.
5 - Fire Fighting Measures
EXTINGUISHING MEDIA
Suitable: Water spray. Carbon dioxide, dry chemical powder, or
appropriate foam.
SPECIAL RISKS
Specific Hazard(s): Emits toxic fumes under fire conditions.
SPECIAL PROTECTIVE EQUIPMENT FOR FIREFIGHTERS
Wear self-contained breathing apparatus and protective clothing
to prevent contact with skin and eyes.
6 - Accidental Release Measures
PERSONAL PRECAUTION PROCEDURES TO BE FOLLOWED IN CASE OF LEAK OR SPILL
Evacuate area.
PROCEDURE(S) OF PERSONAL PRECAUTION(S)
SIGMA - C3886 www.sigma-aldrich.com Page 2
Wear self-contained breathing apparatus, rubber boots, and heavy
rubber gloves. Wear disposable coveralls and discard them after
use.
METHODS FOR CLEANING UP
Sweep up, place in a bag and hold for waste disposal. Avoid
raising dust. Ventilate area and wash spill site after material
pickup is complete.
7 - Handling and Storage
HANDLING
Directions for Safe Handling: Do not breathe dust. Do not get in
eyes, on skin, on clothing. Avoid prolonged or repeated exposure.
STORAGE
Conditions of Storage: Keep tightly closed.
SPECIAL REQUIREMENTS: Light sensitive.
8 - Exposure Controls / Personal Protection
ENGINEERING CONTROLS
Use only in a chemical fume hood. Safety shower and eye bath.
GENERAL HYGIENE MEASURES
Wash contaminated clothing before reuse. Wash thoroughly after
handling.
PERSONAL PROTECTIVE EQUIPMENT
Respiratory Protection: Government approved respirator in
nonventilated areas and/or for exposure above the TLV or PEL.
Hand Protection: Compatible chemical-resistant gloves.
Eye Protection: Chemical safety goggles.
9 - Physical and Chemical Properties
Appearance Color: Light blue
Form: Fine crystals
Property Value At Temperature or Pressure
pH N/A
BP/BP Range N/A
MP/MP Range 173 掳C
Flash Point N/A
Flammability N/A
Autoignition Temp N/A
Oxidizing Properties N/A
Explosive Properties N/A
Explosion Limits N/A
Vapor Pressure N/A
SG/Density N/A
Partition Coefficient N/A
Viscosity N/A
Vapor Density N/A
Saturated Vapor Conc. N/A
Evaporation Rate N/A
Bulk Density N/A
Decomposition Temp. N/A
Solvent Content N/A
Water Content N/A
SIGMA - C3886 www.sigma-aldrich.com Page 3
Surface Tension N/A
Conductivity N/A
Miscellaneous Data N/A
Solubility Solvent: 5 mg/ml EtOH Clear
10 - Stability and Reactivity
STABILITY
Stable: Stable.
Materials to Avoid: Strong oxidizing agents, Strong reducing
agents.
HAZARDOUS DECOMPOSITION PRODUCTS
Hazardous Decomposition Products: Carbon monoxide, Carbon dioxide,
Nitrogen oxides.
HAZARDOUS POLYMERIZATION
Hazardous Polymerization: Will not occur
11 - Toxicological Information
RTECS NUMBER: BO9000000
ACUTE TOXICITY
LD50
Oral
Rat
420 mg/kg
LD50
Intraperitoneal
Rat
8900 UG/KG
LD50
Oral
Mouse
96 mg/kg
LD50
Intraperitoneal
Mouse
5100 UG/KG
LD50
Oral
Rabbit
150 mg/kg
LD50
Intraperitoneal
Rabbit
5 MG/KG
LD50
Intraduodenal
Rabbit
160 MG/KG
IRRITATION DATA
SIGMA - C3886 www.sigma-aldrich.com Page 4
Skin
Human
3 mg
3D
I
Remarks: Mild irritation effect
Skin
Human
2 mg
2D
I
Remarks: Mild irritation effect
Skin
Guinea pig
6 mg
3D
I
SIGNS AND SYMPTOMS OF EXPOSURE
Prolonged exposure can cause: Nausea, headache, and vomiting.
ROUTE OF EXPOSURE
Skin Contact: Causes skin irritation.
Skin Absorption: May be harmful if absorbed through the skin.
Eye Contact: Causes severe eye irritation.
Inhalation: May be harmful if inhaled. Material is irritating to
mucous membranes and upper respiratory tract.
Ingestion: Harmful if swallowed.
CHRONIC EXPOSURE - CARCINOGEN
Result: This product is or contains a component that has been
reported to be probably carcinogenic based on its IARC, OSHA,
ACGIH, NTP, or EPA classification.
Rat
Route of Application: Oral
Exposure Time: 2Y
Result: Tumorigenic:Carcinogenic by RTECS criteria.
Endocrine:Thyroid tumors.
Mouse
Route of Application: Oral
Exposure Time: 2Y
Result: Tumorigenic:Carcinogenic by RTECS criteria. Kidney,
Ureter, Bladder:Tumors. Tumorigenic Effects: 0varian tumors.
CHRONIC EXPOSURE - MUTAGEN
Result: May alter genetic material.
Human
10 UMOL/L
Cell Type: HeLa cell
DNA inhibition
Human
500 UG/L
Cell Type: HeLa cell
Cytogenetic analysis
Human
SIGMA - C3886 www.sigma-aldrich.com Page 5
500 UG/L
Cell Type: lymphocyte
Cytogenetic analysis
Rat
10 NMOL/L
Cell Type: liver
DNA inhibition
Mouse
1 MG/KG
Cell Type: lymphocyte
DNA damage
Hamster
500 UG/L
Cell Type: ovary
Cytogenetic analysis
Mammal
10 PPH
Cell Type: lymphocyte
DNA
Mammal
500 UG/L
Cell Type: Other cell types
Cytogenetic analysis
Non-mammalian
500 UG/L
Cell Type: Other cell types
Cytogenetic analysis
ZINCL
Histidine reversion (Ames)
CHRONIC EXPOSURE - TERATOGEN
Species: Rat
Dose: 100 MG/KG
Route of Application: Oral
Exposure Time: (6-15D PREG)
Result: Specific Developmental Abnormalities: Musculoskeletal
system. Specific Developmental Abnormalities: Urogenital system.
Species: Rat
Dose: 100 MG/KG
Route of Application: Oral
Exposure Time: (6-15D PREG)
Result: Specific Developmental Abnormalities: Other
developmental abnormalities.
Species: Rabbit
Dose: 7 MG/KG
Route of Application: Oral
Exposure Time: (6-19D PREG)
Result: Effects on Embryo or Fetus: Fetotoxicity (except death,
e.g., stunted fetus).
CHRONIC EXPOSURE - REPRODUCTIVE HAZARD
SIGMA - C3886 www.sigma-aldrich.com Page 6
Species: Rat
Dose: 40 MG/KG
Route of Application: Oral
Exposure Time: (6-15D PREG)
Result: Effects on Fertility: Post-implantation mortality (e.g.,
dead and/or resorbed implants per total number of implants).
Species: Rabbit
Dose: 7 MG/KG
Route of Application: Oral
Exposure Time: (6-19D PREG)
Result: Effects on Fertility: Post-implantation mortality (e.g.,
dead and/or resorbed implants per total number of implants).
Effects on Embryo or Fetus: Fetotoxicity (except death, e.g.,
stunted fetus).
12 - Ecological Information
No data available.
13 - Disposal Considerations
SUBSTANCE DISPOSAL
Contact a licensed professional waste disposal service to dispose
of this material. Observe all federal, state, and local
environmental regulations.
14 - Transport Information
RID/ADR
UN#: 3077
Class: 9
PG: III
Proper Shipping Name: Environmentally hazardous
substance, solid, n.o.s
IMDG
UN#: 3077
Class: 9
PG: III
Proper Shipping Name: Environmentally hazardous
substance, solid, n.o.s
Marine Pollutant: No
Severe Marine Pollutant: No
Technical Name: Required
IATA
UN#: 3077
Class: 9
PG: III
Proper Shipping Name: Environmentally hazardous
substance, solid, n.o.s
Inhalation Packing Group I: No
Technical Name: Required
15 - Regulatory Information
CLASSIFICATION AND LABELING ACCORDING TO EU DIRECTIVES
INDICATION OF DANGER: T N
Toxic. Dangerous for the environment.
R-PHRASES: 45 22 41 50/53
May cause cancer. Also harmful if swallowed. Risk of serious
SIGMA - C3886 www.sigma-aldrich.com Page 7
damage to eyes. Very toxic to aquatic organisms, may cause
long-term adverse effects in the aquatic environment.
S-PHRASES: 53 26 39 45 60 61
Restricted to professional users. Attention - Avoid exposure -
obtain special instructions before use. In case of contact with
eyes, rinse immediately with plenty of water and seek medical
advice. Wear eye/face protection. In case of accident or if you
feel unwell, seek medical advice immediately (show the label
where possible). This material and its container must be
disposed of as hazardous waste. Avoid release to the
environment. Refer to special instructions/safety data sheets.
SWITZERLAND
SWISS POISON CLASS: 3
16 - Other Information
WARRANTY
The above information is believed to be correct but does not
purport to be all inclusive and shall be used only as a guide. The
information in this document is based on the present state of our
knowledge and is applicable to the product with regard to
appropriate safety precautions. It does not represent any
guarantee of the properties of the product. Sigma-Aldrich Inc.,
shall not be held liable for any damage resulting from handling or
from contact with the above product. See reverse side of invoice
or packing slip for additional terms and conditions of sale.
Copyright 2005 Sigma-Aldrich Co. License granted to make unlimited
paper copies for internal use only.
DISCLAIMER
For R&D use only. Not for drug, household or other uses.
SIGMA - C3886 www.sigma-aldrich.com Page 8
|