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MSDS Material Safety Data Sheet
CAS

4368-28-9

File Name: 4368-28-9.asp

                              SIGMA-ALDRICH

Material Safety Data Sheet

Date Printed: 31/MAY/2005
Date Updated: 10/FEB/2005
Version 1.4
According to 91/155/EEC

Classified as Hazardous according to the criteria of EU Annex 1 and
NOHSC.

1 - Product and Company Information

Product Name TETRODOTOXIN WITHOUT CITRATE BUFFER
Product Number T8024

Company Sigma-Aldrich Pty, Ltd
Unit 2, 14 Anella Avenue
Castle Hill NSW 1765
Australia
Technical Phone # +61 2 9841 0555
Fax +61 2 9841 0500
Emergency Phone # +61 2 9841 0566

2 - Composition/Information on Ingredients

Product Name CAS # EC no Annex I
Index Number
TETRODOTOXIN 4368-28-9 224-458-8 None

Formula C11H17N3O8
Molecular Weight 319.3 AMU
Synonyms Fugu poison * Maculotoxin * Spheroidine *
Tarichatoxin * Tetrodontoxin * Tetrodotoxin *
Tetrodoxin * TTX

3 - Hazards Identification

SPECIAL INDICATION OF HAZARDS TO HUMANS AND THE ENVIRONMENT
Very toxic by inhalation, in contact with skin and if swallowed.


4 - First Aid Measures

AFTER INHALATION
If inhaled, remove to fresh air. If not breathing give
artificial respiration. If breathing is difficult, give oxygen.

AFTER SKIN CONTACT
In case of skin contact, flush with copious amounts of water for
at least 15 minutes. Remove contaminated clothing and shoes.
Call a physician.

AFTER EYE CONTACT
In case of contact with eyes, flush with copious amounts of
water for at least 15 minutes. Assure adequate flushing by
separating the eyelids with fingers. Call a physician.

AFTER INGESTION
If swallowed, wash out mouth with water provided person is
conscious. Call a physician immediately.
5 - Fire Fighting Measures

EXTINGUISHING MEDIA
Suitable: Water spray. Carbon dioxide, dry chemical powder, or
appropriate foam.

SPECIAL RISKS
Specific Hazard(s): Emits toxic fumes under fire conditions.

SPECIAL PROTECTIVE EQUIPMENT FOR FIREFIGHTERS
Wear self-contained breathing apparatus and protective clothing
to prevent contact with skin and eyes.

6 - Accidental Release Measures

PERSONAL PRECAUTION PROCEDURES TO BE FOLLOWED IN CASE OF LEAK OR SPILL
Evacuate area.

PROCEDURE(S) OF PERSONAL PRECAUTION(S)
Wear self-contained breathing apparatus, rubber boots, and heavy
rubber gloves.

METHODS FOR CLEANING UP
Spilled material should be carefully wiped up or moistened with
water and removed. Decontaminate any spill with 10% caustic
solution Ventilate area and wash spill site after material
pickup is complete.

7 - Handling and Storage

HANDLING
Directions for Safe Handling: Do not breathe dust. Do not get in
eyes, on skin, on clothing. Avoid prolonged or repeated exposure.

STORAGE
Conditions of Storage: Keep tightly closed.
Store at 2-8掳C

8 - Exposure Controls / Personal Protection

ENGINEERING CONTROLS
Safety shower and eye bath. Use only in a chemical fume hood.

GENERAL HYGIENE MEASURES
Wash contaminated clothing before reuse. Wash thoroughly after
handling.

PERSONAL PROTECTIVE EQUIPMENT
Respiratory Protection: Government approved respirator.
Hand Protection: Compatible chemical-resistant gloves.
Eye Protection: Chemical safety goggles.

9 - Physical and Chemical Properties

Appearance Physical State: Solid
Color: White
Form: Powder

Property Value At Temperature or Pressure

pH N/A


SIGMA - T8024 www.sigma-aldrich.com Page 2
BP/BP Range N/A
MP/MP Range N/A
Flash Point N/A
Flammability N/A
Autoignition Temp N/A
Oxidizing Properties N/A
Explosive Properties N/A
Explosion Limits N/A
Vapor Pressure N/A
SG/Density N/A
Partition Coefficient N/A
Viscosity N/A
Vapor Density N/A
Saturated Vapor Conc. N/A
Evaporation Rate N/A
Bulk Density N/A
Decomposition Temp. N/A
Solvent Content N/A
Water Content <5%
Surface Tension N/A
Conductivity N/A
Miscellaneous Data N/A
Solubility N/A

10 - Stability and Reactivity

STABILITY
Stable: Stable.
Materials to Avoid: Strong oxidizing agents.

HAZARDOUS DECOMPOSITION PRODUCTS
Hazardous Decomposition Products: Carbon monoxide, Carbon dioxide,
Nitrogen oxides.

HAZARDOUS POLYMERIZATION
Hazardous Polymerization: Will not occur

11 - Toxicological Information

RTECS NUMBER: IO1450000

ACUTE TOXICITY

LD50
Oral
Mouse
0.334 mg/kg

LD50
Intraperitoneal
Mouse
8 UG/KG

LD50
Subcutaneous
Mouse
8 UG/KG

LD50
Intravenous
Mouse
7300 NG/KG


SIGMA - T8024 www.sigma-aldrich.com Page 3
Remarks: Behavioral:Convulsions or effect on seizure threshold.

LD50
Intracerebral
Mouse
600 NG/KG
Remarks: Behavioral:Convulsions or effect on seizure threshold.
Behavioral:Ataxia.

LD50
Intraperitoneal
Frog
14 UG/KG
Remarks: Peripheral Nerve and Sensation:Spastic paralysis with
or without sensory change.

SIGNS AND SYMPTOMS OF EXPOSURE
Tetrodotoxin is among the most toxic substances known to man.
Death can occur within 30 minutes. It is extremely dangerous by
ingestion, inhalation and skin absorption, or if it enters the
bloodstream in any manner. Symptoms of poisoning which include
numbness, tingling of the lips and inner mouth surfaces,
weakness, paralysis of the limbs and chest muscles, and a drop
in blood pressure have been reported within as little as 10
minutes after exposure. To the best of our knowledge, the
chemical, physical, and toxicological properties have not been
thoroughly investigated.

ROUTE OF EXPOSURE
Skin Contact: May cause skin irritation.
Skin Absorption: May be fatal if absorbed through skin.
Eye Contact: May cause eye irritation.
Inhalation: May be fatal if inhaled. Material may be irritating
to mucous membranes and upper respiratory tract.
Ingestion: May be fatal if swallowed.

TARGET ORGAN INFORMATION
Nerves. Skeletal muscle.

12 - Ecological Information

No data available.

13 - Disposal Considerations

SUBSTANCE DISPOSAL
Contact a licensed professional waste disposal service to dispose
of this material. After use, utensils and the toxin solution
should be soaked overnight or boiled for 20 minutes in an excess
amount of 2% sodium hydroxide solution or sodium hypochlorite (1%
available chlorine) solution in a hood or well ventilated area.
Dissolve or mix the material with a combustible solvent and burn
in a chemical incinerator equipped with an afterburner and
scrubber. Observe all federal, state, and local environmental
regulations.

14 - Transport Information

RID/ADR
UN#: 3462
Class: 6.1
PG: I


SIGMA - T8024 www.sigma-aldrich.com Page 4
Proper Shipping Name: Toxins, extracted from living
sources, solid, n.o.s.

IMDG
UN#: 3462
Class: 6.1
PG: I
Proper Shipping Name: Toxins, extracted from living
sources, solid, n.o.s.
Marine Pollutant: No
Severe Marine Pollutant: No
Technical Name: Required

IATA
UN#: 3462
Class: 6.1
PG: I
Proper Shipping Name: Toxins, extracted from living
sources, solid, n.o.s.
Inhalation Packing Group I: No
Technical Name: Required

15 - Regulatory Information

CLASSIFICATION AND LABELING ACCORDING TO EU DIRECTIVES
INDICATION OF DANGER: T+
Very toxic.
R-PHRASES: 26/27/28
Very toxic by inhalation, in contact with skin and if swallowed.
S-PHRASES: 22 36/37/39 45
Do not breathe dust. Wear suitable protective clothing, gloves,
and eye/face protection. In case of accident or if you feel
unwell, seek medical advice immediately (show the label where
possible).

COUNTRY SPECIFIC INFORMATION

Germany
WGK: 3

16 - Other Information

WARRANTY
The above information is believed to be correct but does not
purport to be all inclusive and shall be used only as a guide. The
information in this document is based on the present state of our
knowledge and is applicable to the product with regard to
appropriate safety precautions. It does not represent any
guarantee of the properties of the product. Sigma-Aldrich Inc.,
shall not be held liable for any damage resulting from handling or
from contact with the above product. See reverse side of invoice
or packing slip for additional terms and conditions of sale.
Copyright 2005 Sigma-Aldrich Co. License granted to make unlimited
paper copies for internal use only.

DISCLAIMER
For R&D use only. Not for drug, household or other uses.




SIGMA - T8024 www.sigma-aldrich.com Page 5

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