HEPES
sc-29097
Material Safety Data Sheet
Hazard Alert Code Key: EXTREME HIGH MODERATE LOW
Section 1 - CHEMICAL PRODUCT AND COMPANY IDENTIFICATION
PRODUCT NAME
HEPES
SYNONYMS
C8-H18-N2-O4-S, "[4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid]", "[4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid]", "N-[2-
hydroxyethyl]piperazine-N' -[2-ethanesulfonic acid]", "N-[2-hydroxyethyl]piperazine-N' -[2-ethanesulfonic acid]", "physiological buffer"
PRODUCT USE
Buffer, useful in the physiological pH range.
SUPPLIER
Company: Santa Cruz Biotechnology, Inc.
Address:
2145 Delaware Ave
Santa Cruz, CA 95060
Telephone: 800.457.3801 or 831.457.3800
Emergency Tel: Luis Yanez at 831.251.2170
HAZARD RATINGS
Min Max
Flammability: 1
Toxicity: 1
Min/Nil=0
Body Contact: 1
Low=1
Moderate=2
Reactivity: 0
High=3
Chronic: 0 Extreme=4
Section 2 - HAZARDS IDENTIFICATION
STATEMENT OF HAZARDOUS NATURE
NON-HAZARDOUS SUBSTANCE. NON-DANGEROUS GOODS. According to NOHSC Criteria, and ADG Code.
POISONS SCHEDULE
None
RISK SAFETY
None under normal operating conditions. None under normal operating conditions.
Section 3 - COMPOSITION / INFORMATION ON INGREDIENTS
NAME CAS RN %
HEPES 7365-45-9 >98
Section 4 - FIRST AID MEASURES
SWALLOWED
!
Immediately give a glass of water.
HEPES
sc-29097
Material Safety Data Sheet
Hazard Alert Code Key: EXTREME HIGH MODERATE LOW
First aid is not generally required. If in doubt, contact a Poisons Information Centre or a doctor.
EYE
! If this product comes in contact with eyes:
Wash out immediately with water.
If irritation continues, seek medical attention.
Removal of contact lenses after an eye injury should only be undertaken by skilled personnel.
SKIN
! If skin contact occurs:
Immediately remove all contaminated clothing, including footwear.
Flush skin and hair with running water (and soap if available).
Seek medical attention in event of irritation.
INHALED
!
If dust is inhaled, remove from contaminated area.
Encourage patient to blow nose to ensure clear passage of breathing.
If irritation or discomfort persists seek medical attention.
NOTES TO PHYSICIAN
! Treat symptomatically.
Section 5 - FIRE FIGHTING MEASURES
EXTINGUISHING MEDIA
!
Water spray or fog.
Foam.
Dry chemical powder.
BCF (where regulations permit).
Carbon dioxide.
FIRE FIGHTING
!
Alert Fire Brigade and tell them location and nature of hazard.
Wear breathing apparatus plus protective gloves for fire only.
Prevent, by any means available, spillage from entering drains or water courses.
Use fire fighting procedures suitable for surrounding area.
DO NOT approach containers suspected to be hot.
Cool fire exposed containers with water spray from a protected location.
If safe to do so, remove containers from path of fire.
Equipment should be thoroughly decontaminated after use.
FIRE/EXPLOSION HAZARD
!
Combustible.
Slight fire hazard when exposed to heat or flame.
Heating may cause expansion or decomposition leading to violent rupture of containers.
On combustion, may emit toxic fumes of carbon monoxide (CO).
May emit acrid smoke.
Mists containing combustible materials may be explosive.
Other combustion products include: carbon dioxide (CO2), sulfur oxides (SOx), ammonia and nitrogen oxides (NOx).
HAZCHEM
None
PERSONAL PROTECTION
Glasses: Respirator:
Chemical goggles. Particulate
HEPES
sc-29097
Material Safety Data Sheet
Hazard Alert Code Key: EXTREME HIGH MODERATE LOW
Section 6 - ACCIDENTAL RELEASE MEASURES
EMERGENCY PROCEDURES
MINOR SPILLS
!
Clean up all spills immediately.
Avoid contact with skin and eyes.
Wear protective clothing, gloves, safety glasses and dust respirator.
Use dry clean up procedures and avoid generating dust.
Sweep up or
Vacuum up (consider explosion-proof machines designed to be grounded during storage and use).
Place in clean drum then flush area with water.
MAJOR SPILLS
!
Clear area of personnel and move upwind.
Alert Fire Brigade and tell them location and nature of hazard.
Control personal contact by using protective equipment and dust respirator.
Prevent spillage from entering drains, sewers or water courses.
Avoid generating dust.
Sweep, shovel up. Recover product wherever possible.
Put residues in labelled plastic bags or other containers for disposal.
If contamination of drains or waterways occurs, advise emergency services.
Personal Protective Equipment advice is contained in Section 8 of the MSDS.
Section 7 - HANDLING AND STORAGE
PROCEDURE FOR HANDLING
! Avoid generating and breathing dust.
Limit all unnecessary personal contact.
Wear protective clothing when risk of exposure occurs.
Use in a well-ventilated area.
Avoid contact with incompatible materials.
When handling, DO NOT eat, drink or smoke.
Keep containers securely sealed when not in use.
Avoid physical damage to containers.
Always wash hands with soap and water after handling.
Work clothes should be laundered separately.
Use good occupational work practice.
Observe manufacturer's storing and handling recommendations.
Atmosphere should be regularly checked against established exposure standards to ensure safe working conditions are maintained.
SUITABLE CONTAINER
!
Glass container is suitable for laboratory quantities
Plastic container.
Multi-ply woven plastic or paper bag with sealed plastic liner
NOTE: Bags should be stacked, blocked, interlocked, and limited in height so that they are stable and secure against sliding or collapse.
Check that containers are clearly labelled
Packaging as recommended by manufacturer.
STORAGE INCOMPATIBILITY
Avoid storage with oxidising agents.
STORAGE REQUIREMENTS
!
Store in original containers.
HEPES
sc-29097
Material Safety Data Sheet
Hazard Alert Code Key: EXTREME HIGH MODERATE LOW
Keep containers securely sealed.
No smoking, naked lights or ignition sources.
Store in a cool, dry, well-ventilated area.
Store away from incompatible materials and foodstuff containers.
Protect containers against physical damage and check regularly for leaks.
Observe manufacturer's storing and handling recommendations.
SAFE STORAGE WITH OTHER CLASSIFIED CHEMICALS
+ + + + X +
X: Must not be stored together
O: May be stored together with specific preventions
+: May be stored together
Section 8 - EXPOSURE CONTROLS / PERSONAL PROTECTION
EXPOSURE CONTROLS
The following materials had no OELs on our records
?HEPES: CAS:7365-45-9
PERSONAL PROTECTION
EYE
!
Safety glasses with side shields
Chemical goggles.
Contact lenses may pose a special hazard; soft contact lenses may absorb and concentrate irritants. A written policy document,
describing the wearing of lens or restrictions on use, should be created for each workplace or task. This should include a review of lens
absorption and adsorption for the class of chemicals in use and an account of injury experience. Medical and first-aid personnel should be
trained in their removal and suitable equipment should be readily available. In the event of chemical exposure, begin eye irrigation
immediately and remove contact lens as soon as practicable. Lens should be removed at the first signs of eye redness or irritation - lens
should be removed in a clean environment only after workers have washed hands thoroughly. [CDC NIOSH Current Intelligence Bulletin
59]
HANDS/FEET
! Wear general protective gloves: i.e. Disposable polythene gloves or Cotton gloves or Light weight rubber gloves, with Barrier cream
preferably Safety footwear.
OTHER
!
Overalls.
Barrier cream
Eyewash unit.
RESPIRATOR
!
HEPES
sc-29097
Material Safety Data Sheet
Hazard Alert Code Key: EXTREME HIGH MODERATE LOW
Protection Factor Half-Face Respirator Full-Face Respirator Powered Air Respirator
10 x ES P1 Air-line* -- PAPR-P1 -
50 x ES Air-line** P2 PAPR-P2
100 x ES - P3 -
Air-line* -
100+ x ES - Air-line** PAPR-P3
* - Negative pressure demand ** - Continuous flow.
The local concentration of material, quantity and conditions of use determine the type of personal protective equipment required.
For further information consult site specific CHEMWATCH data (if available), or your Occupational Health and Safety Advisor.
ENGINEERING CONTROLS
! Use in a well-ventilated area.
Local exhaust ventilation is required where solids are handled as powders or crystals; even when particulates are relatively large, a
certain proportion will be powdered by mutual friction.
Exhaust ventilation should be designed to prevent accumulation and recirculation of particulates in the workplace.
If in spite of local exhaust an adverse concentration of the substance in air could occur, respiratory protection should be considered. Such
protection might consist of:
(a): particle dust respirators, if necessary, combined with an absorption cartridge;
(b): filter respirators with absorption cartridge or canister of the right type;
(c): fresh-air hoods or masks
Build-up of electrostatic charge on the dust particle, may be prevented by bonding and grounding.
Powder handling equipment such as dust collectors, dryers and mills may require additional protection measures such as explosion
venting.
Air contaminants generated in the workplace possess varying "escape" velocities which, in turn, determine the "capture velocities" of fresh
circulating air required to efficiently remove the contaminant.
Type of Contaminant: Air Speed:
direct spray, spray painting in shallow booths, drum filling, conveyer
loading, crusher dusts, gas discharge (active generation into zone of 1-2.5 m/s (200-500 f/min.)
rapid air motion)
grinding, abrasive blasting, tumbling, high speed wheel generated
dusts (released at high initial velocity into zone of very high rapid air 2.5-10 m/s (500-2000 f/min.)
motion).
Within each range the appropriate value depends on:
Lower end of the range Upper end of the range
1: Room air currents minimal or favourable to capture 1: Disturbing room air currents
2: Contaminants of low toxicity or of nuisance value only 2: Contaminants of high toxicity
3: Intermittent, low production. 3: High production, heavy use
4: Large hood or large air mass in motion 4: Small hood-local control only
Simple theory shows that air velocity falls rapidly with distance away from the opening of a simple extraction pipe. Velocity generally
decreases with the square of distance from the extraction point (in simple cases). Therefore the air speed at the extraction point should be
adjusted, accordingly, after reference to distance from the contaminating source. The air velocity at the extraction fan, for example, should be
a minimum of 4-10 m/s (800-2000 f/min) for extraction of crusher dusts generated 2 metres distant from the extraction point. Other
mechanical considerations, producing performance deficits within the extraction apparatus, make it essential that theoretical air velocities are
multiplied by factors of 10 or more when extraction systems are installed or used.
Section 9 - PHYSICAL AND CHEMICAL PROPERTIES
APPEARANCE
White powder; mixes with water.
PHYSICAL PROPERTIES
Solid.
Mixes with water.
State Divided solid Molecular Weight 238.31
Melting Range (癈) 234 (decomp) Boiling Range (癈) Not applicable
Solubility in water (g/L) Miscible Flash Point (癈) 123
HEPES
sc-29097
Material Safety Data Sheet
Hazard Alert Code Key: EXTREME HIGH MODERATE LOW
pH (1% solution) 7 approx. Decomposition Temp (癈) Not available
pH (as supplied) Not applicable Autoignition Temp (癈) Not available
Vapour Pressure (kPa) Negligible Upper Explosive Limit (%) Not available
Specific Gravity (water=1) Not available Lower Explosive Limit (%) Not available
Relative Vapour Density (air=1) Not applicable Volatile Component (%vol) Negligible
Evaporation Rate Not applicable
Section 10 - CHEMICAL STABILITY
CONDITIONS CONTRIBUTING TO INSTABILITY
!
Presence of incompatible materials.
Product is considered stable.
Hazardous polymerisation will not occur.
For incompatible materials - refer to Section 7 - Handling and Storage.
Section 11 - TOXICOLOGICAL INFORMATION
POTENTIAL HEALTH EFFECTS
ACUTE HEALTH EFFECTS
SWALLOWED
! The material may be mildly discomforting to the gastro-intestinal tract and may be harmful if swallowed in large quantity.
Considered an unlikely route of entry in commercial/industrial environments.
EYE
! The dust may produce eye discomfort causing smarting, pain and redness.
SKIN
! The material may be slightly discomforting to the skin.
INHALED
! Not normally a hazard due to non-volatile nature of product.
The dust is discomforting to the upper respiratory tract.
Persons with impaired respiratory function, airway diseases and conditions such as emphysema or chronic bronchitis, may incur further
disability if excessive concentrations of particulate are inhaled.
If prior damage to the circulatory or nervous systems has occurred or if kidney damage has been sustained, proper screenings should be
conducted on individuals who may be exposed to further risk if handling and use of the material result
in excessive exposures.
CHRONIC HEALTH EFFECTS
! Principal routes of exposure are usually by skin contact and inhalation of generated dust.
As with any chemical product, contact with unprotected bare skin; inhalation of vapour, mist or dust in work place atmosphere; or ingestion in
any form, should be avoided by observing good occupational work practice.
TOXICITY AND IRRITATION
! No data of toxicological significance identified in literature search.
None available.
Section 12 - ECOLOGICAL INFORMATION
Refer to data for ingredients, which follows:
HEPES:
Ecotoxicity
Ingredient Persistence: Water/Soil Persistence: Air Bioaccumulation Mobility
HEPES HIGH LOW HIGH
HEPES
sc-29097
Material Safety Data Sheet
Hazard Alert Code Key: EXTREME HIGH MODERATE LOW
Section 13 - DISPOSAL CONSIDERATIONS
!
Consult manufacturer for recycling options and recycle where possible .
Consult State Land Waste Management Authority for disposal.
Incinerate residue at an approved site.
Recycle containers if possible, or dispose of in an authorised landfill.
Section 14 - TRANSPORTATION INFORMATION
HAZCHEM:
None (ADG6)
NOT REGULATED FOR TRANSPORT OF DANGEROUS GOODS: UN, IATA, IMDG
Section 15 - REGULATORY INFORMATION
POISONS SCHEDULE
None
REGULATIONS
HEPES (CAS: 7365-45-9) is found on the following regulatory lists;
"Australia Inventory of Chemical Substances (AICS)"
Section 16 - OTHER INFORMATION
! Classification of the preparation and its individual components has drawn on official and authoritative sources as well as independent
review by the Chemwatch Classification committee using available literature references.
A list of reference resources used to assist the committee may be found at:
www.chemwatch.net/references.
! The (M)SDS is a Hazard Communication tool and should be used to assist in the Risk Assessment. Many factors determine whether the
reported Hazards are Risks in the workplace or other settings. Risks may be determined by reference to Exposures Scenarios. Scale of use,
frequency of use and current or available engineering controls must be considered.
This document is copyright. Apart from any fair dealing for the purposes of private study, research, review or
criticism, as permitted under the Copyright Act, no part may be reproduced by any process without written
permission from CHEMWATCH. TEL (+61 3) 9572 4700.
Issue Date: 12-May-2005
Print Date:5-Feb-2010
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