Material Safety Data Sheet
Revision: 08/11/2005
Hazard information is provided for compliance with both the UK Chemicals
(Hazard Information and Packaging) (CHIP) Regulations and the US Hazard
Communication Standard (HCS)
IDENTIFICATION OF THE PRODUCT NAME: PRODUCT CODE: EEC NUMBER:
SUBSTANCE/PREPARATION DNA Sequencing Gel Mix, 6%, TBE Buffer 72990 None
AND COMPANY
SUPPLIER: Emergency Contact:
USB Corporation, 26111 Miles Road, Cleveland, Ohio 44128 Phone: (216) 765-5000 Chemtrec (800) 424-9300
Please visit our website at www.usbweb.com for contact information on USB Outside USA & Canada 703 527 3887
product distributors within your area.
COMPOSITION/
HAZARDOUS COMPONENTS HAZARD CAS NO. %WT TLV
Urea 57-13-6 ~81% ---
Acrylamide 79-06-1 ~15% 0.03mg/m3
Tris 77-86-1 ~1.9% --
Boric Acid 10043-35-3 ~1% --
For the mixture/preparation: CHIP R & S Phrases
R:45 May cause cancer.
R:46 May cause heritable genetic damage.
R:20/21 Harmful by inhalation and in contact with skin.
R:25 Toxic if swallowed.
R:36/38 Irritating to eyes and skin.
R:43 May cause sensitization by skin contact.
R:48/23/24/25 Toxic: danger of serious damage to health by prolonged
exposure through inhalation, in contact with skin and if swallowed.
R:62 Possible risk of impaired fertility.
S:53 Avoid exposure - Obtain special instructions before use.
S:45 In case of accident or if you feel unwell seek medical advice
immediately (show the label where possible).
HAZARDS IDENTIFICATION CHIP
Acrylamide: Carcinogen Category 2, Mutagen Category 2, Reprod. Category 3 and Toxic. Acrylamide, Urea, Boric
Acid and Tris: Irritant.
HCS
Acrylamide: Toxic, Suspected Carcinogen, Reprod. Hazard and Neurotoxin. Acrylamide, Urea, Boric Acid and Tris:
Irritant.
FIRST-AID MEASURES EYES: Flush with water for 15 minutes. Seek medical advice if irritation persists.
SKIN: Flush with water, then wash thoroughly with soap and water. Remove contaminated clothing and wash
before reuse. Seek medical attention if irritation persists.
INHALATION: Remove the victim from exposure and move to fresh air. If breathing is difficult, give oxygen. If
not breathing, give artificial respiration. Keep victim quiet and warm. Seek immediate medical attention.
INGESTION: Drink water and seek immediate medical attention. Avoid alcoholic beverages. Never give
anything by mouth to an unconscious person.
FIRE-FIGHTING INFORMATION Use media suitable to extinguish the supporting or surrounding fire. Wear NIOSH (or equivalent) approved self
contained breathing apparatus. For small fires only: use carbon dioxide, dry powder or foam. Container explosion
may occur under fire conditions. Emits toxic fumes under fire conditions.
Flash Point = No data available.
ACCIDENTAL RELEASE MEASURES Wear appropriate personal protective equipment and clothing including lab coat, safety glasses, gloves and NIOSH-
approved respirator. Collect in a manner that does not create dust and place in a suitable waste container. Avoid
contact of material with skin or eyes. Use adequate ventilation.
HANDLING AND STORAGE Wear appropriate personal protective equipment and clothing including lab coat, safety glasses, gloves and NIOSH-
approved respirator. Avoid contact of material with skin or eyes. Use adequate ventilation. Avoid excess heat and
direct sunlight. Store ambient away from incompatible materials.
MSDS information may be continued on back of page.
PERSONAL PROTECTION Wear appropriate personal protective equipment and clothing including lab coat, safety glasses, gloves and NIOSH-
approved respirator. A qualified industrial hygienist should evaluate the need for respiratory protection. Use
respiratory protection approved by NIOSH (or equivalent) and appropriate to the hazard. Avoid contact of material
with skin or eyes. Mechanical ventilation or local exhaust as needed to control exposure to dust, vapors or mists.
Access to a safety shower and eye-wash.
PHYSICAL AND CHEMICAL Appearance: White crystalline powder Chemical Formula: Mixture
PROPERTIES Vapor Pressure: No data available Boiling Point: No data available
STABILITY AND REACTIVITY Aqueous solutions of Acrylamide are unstable if heated. May polymerize at high temperatures. Avoid excess heat
and direct sunlight. Incompatible with acids, bases, oxidizing agents, reducing agents, iron and iron salts, copper,
aluminum, brass, bisulfites, peroxides and free radical initiators. Hazardous decomposition products include carbon
and nitrogen oxides and ammonia.
TOXICOLOGICAL INFORMATION EFFECTS OF OVEREXPOSURE:
For Acylamide: Oral Rat LD50 = 124 mg/kg (1975). Complete irritation, mutation, tumorigenic, reproductive
effects, and toxicity data for Acrylamide listed in RTECS under AS3325000.
SKIN AND EYES: Aqueous solutions are mildly irritating to the eyes and skin. Chronic skin contact may
result in peeling and redness of skin. May cause allergic reaction. Excessive skin contact may lead to
significant absorption through the skin, resulting in systemic symptoms listed under ingestion.
INHALATION: Airborne Acrylamide, dust or vapors, is readily absorbed through the lungs and may be
cumulative leading to systemic symptoms listed under ingestion. May cause allergic reaction.
INGESTION: Ingestion or excessive exposure may cause neuropathy exemplified by muscular weakness,
ataxia, erythema, excessive sweating of the hands and arms, cold fingers and hands, numbness,
parenthesis and fatigue. Acrylamide is a human neurotoxin. Acrylamide has been shown to be a
mutagenic agent in both in-vivo and in-vitro tests. May cause cancer and heritable genetic damage.
May cause reproductive disorders. Toxic by inhalation, in contact with skin and if swallowed.
For Urea: Oral Rat LD50 = 8471 mg/kg (1986). Complete irritation, mutation, tumorigenic, reproductive effects,
and toxicity data for Urea listed in RTECS under YR6250000.
EYES: Contact may cause irritation and slight corneal injury.
SKIN: Contact may cause irritation and/or allergic reaction.
INHALATION: May cause irritation to mucous membranes and upper respiratory tract.
INGESTION: Chronic ingestion or excessive dosage may cause nausea, vomiting, gastrointestinal tract
irritation, venous thrombis and phlebitis. Laboratory experiments have shown mutagenic effects.
For Tris: Oral Rat LD50 = 5900 mg/kg. Complete toxicity data for Tris listed in RTECS under TY2900000.
EYES: Contact may cause irritation and slight corneal injury.
SKIN: Contact may cause irritation and/or allergic reaction.
INHALATION: May cause irritation to mucous membranes and upper respiratory tract.
INGESTION: Chronic ingestion or excessive dosage may cause nausea, vomiting and diarrhea. Large
amounts may cause weakness, collapse or coma. Ingestion may cause irritation to mouth, throat and
esophagus and gastrointestinal tract.
For Boric Acid: Oral Rat LD50 = 2660 mg/kg (1945). Complete irritation, mutation, reproductive effects and
toxicity data for Boric Acid listed in RTECS under ED4550000.
EYES: Contact may cause irritation. Prolonged exposure may cause conjunctivitis and edema.
SKIN: Contact may cause mild irritation. May be absorbed through the skin to cause systemic effects
including nausea, vomiting, diarrhea, abdominal cramps, erythematous lesions on skin and mucous
membranes, circulatory collapse, tachycardia, cyanosis, delirium, convulsions and coma.
INHALATION: May cause irritation of mucous membranes and possible systemic effects (see Skin above).
INGESTION: May cause systemic effects listed under skin as well as anorexia, reddening of the tongue, loss
of hair, palpebral edema, gastrointestinal disturbances, kidney and liver damage.
Carcinogenicity data for Acrylamide: IARC Cancer Review: Animal - Sufficient Evidence (1994).
Human - Inadequate Evidence (1994). Group 2A - Agent is probably carcinogenic to Humans.
NTP Carcinogenesis Studies: Reasonably anticipated to be Human Carcinogen (1998).
Tumorigenic data: For Acrylamide and Urea: Carcinogenic and Neoplastic by RTECS criteria.
ECOLOGICAL INFORMATION No information available.
DISPOSAL CONSIDERATIONS Dispose of material in accordance with applicable local, state, and federal regulations.
TRANSPORTATION INFORMATION US DOT / IATA: Acrylamide, solid, class 6.1, UN2074, PG III. Label: Toxic.
REGULATORY INFORMATION RCRA - For Acrylamide: Hazardous Waste (U007).
OSHA - For Acrylamide: Air Contaminant.
SARA 302 - For Acrylamide: Extremely Hazardous Chemical.
SARA 313 - For Acrylamide: Toxic Chemical.
EPA TSCA Section 8(b) - For Acrylamide, Urea, Boric Acid and Tris: Chemical Inventory.
Exposure Limits - For Acrylamide: ACGIH TLV-TWA: 0.03 mg/m3 (skin). OSHA PEL: 8H TWA 0.3 mg/m3
(skin). NIOSH REL to Acrylamide-air: 10H CA TWA 0.03 mg/m3 (Sk).
California Proposition 65 - This product is or contains chemical(s) known to the State of California to cause
cancer.
This data sheet is based upon information believed to be reliable. The Company makes no statement or warranty as to the accuracy or completeness of the
information contained herein which is offered for your consideration, investigation and verification. Any use of the information contained in this data sheet
must be determined by the user to be in accordance with appropriate applicable regulations.
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