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MSDS Material Safety Data Sheet
CAS

77-86-1 7647-01-0 139-33-3 7732-18-5

File Name: 77-86-1_7647-01-0_139-33-3_7732-18-5.asp

                                 SibEnzyme Ltd.
Ak. Timakov str., 2/12, Novosibirsk, 630117, Russia Tel.: +7 383 333 49 91
E-mail: info@sibenzyme.com Tel/Fax: +7 383 333 68 53
http://www.sibenzyme.com




MATERIAL SAFETY DATA SHEET
(The format of the MSDS complies with Commission Directive 2001/58/EC as amended.)
Revised: 01 December 2006


1. IDENTIFICATION OF THE PREPARATION/SUBSTANCE AND OF THE COMPANY

Preparation Name:

Lambda DNA/Bgl I ?DNA ladder - buffered aqueous solution.

(Supplied with Loading Dye Solution).

Catalogue numbers of the product:
M17, M18, SM18, MM18, LM18 (depending on the quantity).

Use of the preparation:
For laboratory use.
Suitable for use as molecular weight standards for agarose gel electrophoresis.

Company identification:
SibEnzyme Ltd.
2/12 Timakova Str.
630117 Novosibirsk
Russia
Telephone: +7 383 333 4991
Telephone/Fax: +7 383 333 6853
Site: http://www.sibenzyme.com
E-mail: info@sibenzyme.com

Emergency telephone number:
+7 383 333 6853

2. COMPOSITION/ INFORMATION ON INGREDIENTS
Ingredients:
1. Lambda DNA/Bgl I ~ 0.5% (w/v) EC# CAS#
2. Tris-HCl < 1% (w/v)
(mix of Tris (Trometamol) EC#201-064-4 CAS#77-86-1
and Hydrochloric acid EC#231-595-7 CAS#7647-01-0)
3. EDTA (disodium salt) < 0.1% (w/v) EC#205-358-3 CAS#139-33-3
4. Water ~ 99% (v/v) EC#231-791-2 CAS#7732-18-5

1
Ingredients indicated above do not present a health or environmental hazard within the meaning of
Council Directive 67/548/EEC, or their concentrations do not exceed the limits specified in Council
Directive 1999/45/EC, and in Commission Directive 2001/58/EC.

3. HAZARDS IDENTIFICATION

The preparation is not classified as dangerous according to Directive 1999/45/EC or Directive
67/548/EC, and is not labelled with danger symbols or indications of danger.

Hazards deriving from physical-chemical properties:
The data available do not support any physical or chemical hazard.

Human health hazards:
Inappropriate handling may cause formation of aerosols. Inhalation of aerosols may cause
allergic type reactions in sensitized individuals. Prolonged skin contact may cause minor
irritation.
Caution: Avoid inhalation, ingestion, and skin contacts.

Environmental hazards:
The data available do not support any environmental hazard.

4. FIRST-AID MEASURES

Ingestion:
If swallowed, rinse mouth and throat thoroughly with water. Consult a doctor in event of any
complaints.

Inhalation:
If inhaled, remove victim from area of exposure. If symptoms of irritation or respiratory
allergy occur, consult a doctor.

Skin contact:
Generally, the product does not irritate the skin. If symptoms of irritation after prolonged
exposure occur, consult a doctor.

Eye contact:
In case of contact, wash out opened eyes with plenty of water for several minutes. Consult a
doctor in event of any complaints.

5. FIRE-FIGHTING MEASURES

The preparation cannot cause fire.

Suitable extinguishing agents:
Water spray, Carbon Dioxide, dry chemical powder or appropriate foam.

Not suitable extinguishing agents:
None.

Special exposure hazards from decomposition products:
Not thoroughly investigated.


2
Protective equipment:
Wear self-contained breathing apparatus and protective clothing.

6. ACCIDENTAL RELEASE MEASURES

Personal precautions:
Wear appropriate gloves (see Section 8), safety glasses to clean small releases/spillage. In
case of vapour/aerosol formation, wear suitable respiratory protection means. Ventilate the
area.

Environmental precautions:
No special measures required.

Methods for cleaning up:
Absorb spillages with liquid-binding material. Ventilate and wash the area after the spillage
is completely removed.

Additional information:
No dangerous substances are released.

7. HANDLING AND STORAGE

Handling:
No further requirements.

Use appropriate personal protection measures (see Section 8). Avoid any contact with the
liquid. Avoid the formation of aerosols. Ventilate area.

Storage:
Store at - 20癈.
None other specific requirements relating to the preparation.

8. EXPOSURE CONTROLS/PERSONAL PROTECTION

Ingredients with limit values that require monitoring at the workplace:
The product does not contain any relevant quantities of materials with critical values that
need to be monitored at the workplace.

General protective and hygienic measures:
The usual precautionary measures are the same as for chemicals handling.

Engineering measures:
None specific requirements relating to the preparation.

Personal protection:
- Respiratory protection: No special measures required.
- Hand protection: Wear rubber gloves. None special requirements to rubber.
- Eye protection: No need to wear glasses (goggles) when ordinary operations with the
preparation. Safety glasses are recommended during certain operations when aerosol
formation is possible.
- Skin protection: The usual precautionary measures accepted in research laboratories, e.g.
protective clothes (gowns). None specific requirements.
3
9. PHYSICAL AND CHEMICAL PROPERTIES

Appearance: Liquid.
Colour: Colourless.
Odour: Odourless.
pH-value at 25癈: 8.0.
Boiling point: 100癈.
Flash Point: Not applicable.
Flammability/autoflammability: The preparation is not flammable/self-igniting.
Explosive properties: The preparation does not possess explosive properties.
Oxidizing properties: Not applicable.
Vapour pressure: Not applicable.
Relative density at 20癈: ~1 g/cm3.
Solubility in/miscibility with water: Fully miscible.
Fat solubility: Not applicable.
Vapour density: Not applicable.
Evaporation rate: Not applicable.
Conductivity: Not applicable.
Viscosity: Not viscous.

10. STABILITY AND REACTIVITY

Stability:
Stable under storage conditions (see Section 7).

Conditions to avoid:
No conditions which may cause dangerous reactions.

Possibility of hazardous reactions:
No dangerous reactions known.

Materials to avoid:
No materials which may cause dangerous reactions.

Hazardous decomposition products:
No dangerous decomposition products known.

Hazardous polymerization:
Will not occur.

11. TOXICOLOGICAL INFORMATION

Routes of exposure:
Inhalation: Inhalation of aerosols may cause allergic type reactions in sensitized
individuals.
Ingestion: Repeated ingestion or excessive dosage may cause irritation of the
gastrointestinal tract.
Skin and eye contact: Generally, the product does not irritate the skin and eyes. Prolonged
skin or eye contact may cause minor irritation.




4
Additional toxicological information:
When used and handled according to specifications, the product does not have any harmful
effects.

The product is not listed in a priority list as foreseen under Council Regulation (EEC) No 793/93 on
the evaluation and control of the risks of the existing substances.

12. ECOLOGICAL INFORMATION

Ecotoxicity:
The preparation is considered not dangerous to the environment with respect to mobility,
persistence and degradability, bioaccumulation, aquatic toxicity and other data relating to
ecotoxicity.

Persistence and degradability:
The preparation is readily biodegradable.

Bioaccumulative potential:
It is considered that the preparation does not accumulate in biota, and does not pass through
the food chain.

Other adverse effects:
Not applicable.

13. DISPOSAL CONSIDERATIONS

The disposal of the product does not present a danger.

No special disposal methods required, except to be in accordance with current local environmental
regulations.

14. TRANSPORT INFORMATION

Special precautions:
None known.

Proper shipping name:
Not dangerous goods according to transport regulations.

National transport regulations:
No additional national transport regulations are known to the supplier.

IMDG: Non-hazardous for sea freight.
ADR: Non-hazardous for road transport.
RID: Non-hazardous for rail transport.
ICAO/IATA: Non-hazardous for air transport.

Other information:
The preparation may be stored in ice during the transportation.




5
15. REGULATORY INFORMATION

US
Labelling according to US Regulations: Caution: Avoid contact and inhalation.
US Regulatory information: SARA LISTED: No.

EU
Labelling according to EU Guidelines: The preparation is not dangerous within the
meaning of Directives 67/548/EC, 1999/45/EC, and 88/379/ECC, and is not labelled with
danger symbols or indications of danger.

Canada
WHMIS classification: This product has been classified in accordance with the hazard
criteria of the CPR, and the MSDS contains all the information required by the CPR.
DSL: No.
NDSL: No.

16. OTHER INFORMATION

The above information is believed to be correct but does not purport to be all-inclusive and shall be
used only as a guide. SibEnzyme shall not be held liable for any damage resulting from handling or
from contact with the above product.




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