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MSDS Material Safety Data Sheet
CAS

314-13-1 26628-22-8 280-57-9 56-81-5

File Name: 314-13-1_26628-22-8_280-57-9_56-81-5.asp

Australian Supplier

In Vitro Technologies Pty. Ltd.
7-9 Summit Road
Noble Park, Melbourne, VIC 3174
T: +61397713700 / 1300 552 003
HS MSD 079/200406
Emergency information: Posions Info. Centre: 131126
Page 1 of 4
MATERIAL SAFETY DATA SHEET


1.0 Product and Company Identification


Product Name: ANCA kits


Product Code: FK016, FK016.1, FK017, FK017.1, FK018,

Manufacturer: The Binding Site Ltd
PO Box 11712
Birmingham
B14 4ZB
England

Tel: +44 (0)121 436 1000


2.0 Composition, Information on Ingredients

Whole kits and individual components contain the following:-

Component Chemical % CAS# EINECS#
Evans Blue Evans Blue 1 314-13-16 206-242-5
containing sodium azide 0.099 26628-22-8 247-852-1
Positive & Sodium azide 0.099 26628-22-8 247-852-1
Negative
Controls
Mounting Media 1,4 Diazaobicyclo (2,22) Octane 2.5 280-57-9 205-999-9
(DABCO) in
Glycerol/PBS solution 97.5 56-81-5 200-289-5
(9parts glycerol to 1 part PBS) (Glycerol) (Glycerol)
PBS Phosphate Buffered Saline N/A N/A
Tissue Slide N/A N/A N/A N/A
Conjugate Sodium azide 0.099 26628-22-8 247-852-1
Evans Blue 0.0002 314-13-16 206-242-5


Evans Blue - Hazard Symbol : T



------------------------------------------------------------------------------------------------------------
HS MSD 079/200406
Page 2 of 4
3.0 Hazard Identification

Evans Blue ?May cause harm to the unborn child.
Harmful by inhalation, in contact with skin and if swallowed.
Possible carcinogen

The mounting media must be treated with care and gloves should be worn when handling.

All other components of this product contain substances that are either non hazardous or at
concentrations below that requiring the issue of an individual specific Material Safety Data
Sheet. However, general safety precautions should be observed and appropriate procedures
used to prevent direct contact with the eyes or skin and to prevent inhalation.


4.0 First Aid Measures

Eyes: Flush with water for at least 15 minutes.

Skin: Flush skin with plenty of soap and water for at least 15 minutes while
removing contaminated clothing and shoes.

Ingestion: Wash mouth out with water. Call a physician

Inhalation: Remove from exposure to fresh air immediately.

Note: If symptoms persist seek medical advice.


5.0 Fire Fighting Measures

As in any fire wear self-contained breathing apparatus and full protective gear. During a
fire irritating and highly toxic gases may be generated by thermal decomposition or
combustion. Use appropriate extinguishers when fire fighting.


6.0 Accidental Release Measure

Clean up area and wash with water and an appropriate cleaning agent. Use appropriate
personal protective equipment (see section 8).


7.0 Handling & Storage

Any material which is of human origin has been tested at donor level and found negative
for hepatitis B surface antigen (HbsAG), antibodies to HIV1 and 2 and HCV. However,
these tests cannot guarantee the absence of infective agents and full safety precautions
should be taken as in the handling of any potentially infective body fluid. Only personnel
adequately trained in such methods should be permitted to perform the procedures.

All products should be stored as per specific instructions.
HS MSD 079/200406
Page 3 of 4

Wash thoroughly after handling. Remove contaminated clothing and wash before re-use.
Avoid ingestion and inhalation.

Equipment should be regularly cleaned as per manufacturers instructions, particularly prior
to maintenance or repair.

8.0 Exposure Controls, Personal Protection

Where appropriate use adequate general or local exhaust ventilation to control airborne
levels.

Protective laboratory clothing should be worn along with other suitable protection (eg
gloves) if appropriate Protective gloves should be worn when handling the mounting
media and Evans Blue.

9.0 Physical and Chemical Properties

No extra information available.

10.0 Stability and Reactivity

For product stability see specific product instructions.

11.0 Toxicological Information

Evans Blue ?Acute Harmful if swallowed, inhaled or absorbed through skin
May cause irritation

Chronic Possible carcinogen
Overexposure may cause reproductive disorders based on
tests with laboratory animals

The chemical, physical and toxicological properties of this product have not been fully
investigated.

12.0 Ecological Information

No environmental hazard is anticipated if the product is handled and disposed of with due
care.

13.0 Disposal Considerations

Use appropriate methods for disposal of kit contents and other consumables used. Observe
all federal, state and local environmental regulations

Some of the components of this kit contain sodium azide & must be handled with caution ?br> Explosive metal azides may be formed with lead or copper plumbing; on disposal of
reagent, flush with a large volume of water to prevent azide build up.
HS MSD 079/200406
Page 4 of 4

14.0 Transport Information

Product is not classified as hazardous for transport

15.0 Regulatory Information

Evans Blue - Toxic
R 61 - May cause harm to the unborn child
R20/21/22 ?Harmful by inhalation, in contact with skin and if swallowed
S 45 ?In case of accident or if you feel unwell, seek medical advice. (Show the label
where possible)
S 36/37/38 ?wear suitable protective clothing, gloves and eye/face protection


16.0 Other Information

All products should be handled only by persons experienced in laboratory techniques.
Storage and stability information is given on the product profile. The products are for `in
vitro' use only and should not be used for `in vivo' for therapeutic purposes.

This information is believed to be correct but does not purport to be all inclusive. The
Binding Site will not be held liable for any damage resulting from handling or from contact
with the above product.

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