1
Material Safety Data Sheet
According to 91/155 EEC
This Material Safety Data Sheet applies to all the Diaclone ELISA kit
1/ Data on components
Regarding components comprised in the kit, the following products are defined as hazardous chemical according the
Directive 67/548/EEC
Accordingly, Material Safety Data Sheet(s) for the following chemical(s) are enclosed:
Sodium Azide: at a final concentration of 0,1-1% is used as a preservative in the following components of the kit:
Standard Diluent Buffer, Standard Diluent Human Serum, Detection Antibody, Biotinylated Antibody Diluent.
ProClin 300: at a final concentration from 0,01-0,1% is used as a preservative in the following components of the kit:
Streptavidin-HRP, HRP Diluent
Sulfuric Acid:at a final concentration 1N in aqueous solution is used in Stop Reagent
Tetramethylbenzidine (TMB): is used as a chromogen in the TMB Solution .
Other chemical contained within these products do not meet definition of a hazardous chemical according EC
Directives.
2/ General information
The above information is believed to be correct but does not purport to be al inclusive and shall be used only as a
guide. DIACLONE SAS shall not be liable for any damages resulting from handling or from contact with the above
products.
Section 1 - Identification of substance
Product Identification: ELISA kit
Standard Diluent Buffer
Standard Diluent Human Serum
Detection Antibody
Biotinylated Antibody Diluent
Manufacturer: DIACLONE SAS
1, Bd Fleming
F-25000 Besan鏾n
France
Tel: +33 3 81 41 38 38
Fax: +33 3 81 41 36 36
e-mail: info@diaclone.com
www.diaclone.com
Section 2 - Data on components
Chemical characterization: Preparation
Hazardous ingredients: % CAS Number EC Number
Sodium Azide 0,1-1% 26628-22-8 247-852-1
T+ , N; 28-32-50/53
Additional information:
The data and advice relate to the concentrations not of the lyophilized powder, but of the prepared solution
Section 3 - Hazard Identification
Hazard Description: not applicable
Information concerning to particular hazards to man and environment: not applicable
Section 4 - First Aid Measures
General information No special measures required
After inhalation Supply fresh air; consult doctor in case of complaints
After skin contact Generally the product does not irritate the skin
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Issue N? July,15 2002 S.Thomas / J. Wijdenes
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Material Safety Data Sheet
According to 91/155 EEC
After eye contact Rinse opened eye for several minutes under running water
After ingestion If symptoms persist consult doctor
Section 5 - Fire Fighting Measures
Suitable extinguishing agents
CO2, powder or water spray. Fight larger fires with water spray or alcohol resistant foam.
Protective equipement No special measures required
Section 6 - Accidental Release Measures
Person-related safety precautions: Not required
Measures for environmental protections
Dilute with plenty of water.
Do not allow to enter sewers / surface or ground water.
Measures for cleaning / collecting
Absorb with liquid-binding material (sand, diatomite, acid binders, universal binders, sawdust).
Section 7- Handling and storage
Handling:
Information for safe handling: Avoid breathing dust, vapor, mist or gas. Avoid contact with eyes, skin and clothing.
Keep container tightly closed. Use with adequate ventilation.
Information about fire - and explosion protection:
The product listed in this MSDS contains sodium azide, a chemical highly toxic in pure form. At product
concentrations, though not classified as hazardous, concentration build-ups of sodium azide may react with lead and
copper plumbing to form highly explosive metal oxides. Flush with large volumes of water to prevent azide build-up in
plumbing.
Storage:
Requirement to be met by storerooms and receptables: Store in a cool, dry, well-ventilated area away from
incompatible substances. Keep away from metals.
Information about storage in one common storage facility: Not required
Further information about storage conditions: None
Storage class:
Class according to regulation on flammable liquids: void
Section 8 - Exposure Controls / Personal Protection
Additional information about design of technical facilities: No further data; see section 7
Ingredients with limit values that require monitoring at the workplace
The product does not contain any relevant quantities of material with critical values that have to be
monitored at the workplace.
Personal protective equipment
General protective and hygienic measures
The usual precautionary measures are to be adhered to when handling chemicals
Respiratory protection: Not required
Protection of hands: Protective gloves
Eye Protection: Goggles recommended during refilling
Section 9 - Physical and Chemical Properties
Form: Liquid
Standard Diluent Detection Biotinylated
Human Serum Antibody Antibody Diluent
Colour: Standard Diluent Buffer
Light yellow opaque yellow colourless blue
Odour: Odourless
Melting point/Melting range: N/A
Boiling point/Boiling range: N/A
Flash point: Not applicable
Autoignition temperature: N/A
Danger of explosion: Product does not present an explosion hazard
Densitiy: N/A
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Issue N? July,15 2002 S.Thomas / J. Wijdenes
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Material Safety Data Sheet
According to 91/155 EEC
Solubility in / Miscibility with
water : fully miscible
Section 10 - Stability and Reactivity
Thermal decomposition: No decomposition if used according to specifications
Dangerous reaction: No dangerous reactions known
Dangerous decompositon products: No dangerous decomposition products known
Section 11 - Toxicological Information
Route of entry: Skin Contact, Eye contact, Inhalation, Ingestion
Effects of acute exposure to Product:
Acute poisoning may cause gastrointestinal irritation and renal failure. May be fatal if inhaled, swallowed or absorbed
through the skin.
Effects of chronic exposure to Product:
Chronic ingestion may result in salicylism which is charcaterized by nausea, vomiting, gastrointestinal ulcers, and
hemorrhagic strokes. Laboratory experiments have shown mutagenic effects. Target organs: Kidneys and cnatral
nervous system.
Exposure Limits Irritancy Sensitization To Product Carcinogenicity
N/A N/A N/A N/A
Terratogenicity Reproductive Toxicity Mutagenicity Synergistic Products
N/A N/A N/A N/A
Section 12 - Ecological Information
General Notes: hazardous for water
Do not allow product to reach ground water, water course or sewage system
Danger to drinking water even if small quantities leak into the ground.
Section 13 - Disposal considerations
Waste disposal: dispose of in accordance with local official environmental regulations
Section 14 - Transport Information
Special shipping information: N/A
Section 15 - Regulations
Labelling according to European labelling guidelines:
Observe the general safety regulations when handling chemicals.
The product is not subject to identification regulations under EC Directives.
Section 16 - Other information
This information is prepared on our present knowledge. However, this shall not constitute a guarantee for any specific
product features and shall not establish a legally valid contractual relationship.
Prepared by: Marketing Department Phone N?+33 3 81 41 38 38 Date: July 02
Approved by / Approuv?par
Issue N? July,15 2002 S.Thomas / J. Wijdenes
4
Material Safety Data Sheet
According to 91/155 EEC
Section 1 - Identification of substance
Product Identification: ELISA kit
HRP Diluent
Streptavidin-HRP
Manufacturer: DIACLONE SAS
1, Bd Fleming
F-25000 Besan鏾n
France
Tel: +33 3 81 41 38 38
Fax: +33 3 81 41 36 36
e-mail: info@diaclone.com
www.diaclone.com
Section 2 - Data on components
Chemical characterization: Preparation
Hazardous ingredients: % CAS Number EC Number
Proclin 300 0,01-0,1% N/A 203-821-4
T ; R 20-41-43-48/24/25
Additional information:
The data and advice relate to the concentrations not of the lyophilized powder, but of the prepared solution
Section 3 - Hazard Identification
Hazard Description: not applicable
Information concerning to particular hazards to man and environment: not applicable
Section 4 - First Aid Measures
General information No special measures required
After inhalation Supply fresh air; consult doctor in case of complaints
After skin contact Generally the product does not irritate the skin
After eye contact Rinse opened eye for several minutes under running water
After ingestion If symptoms persist consult doctor
Section 5 - Fire Fighting Measures
Suitable extinguishing agents
CO2, powder or water spray. Fight larger fires with water spray or alcohol resistant foam.
Protective equipement No special measures required
Section 6 - Accidental Release Measures
Person-related safety precautions: Not required
Measures for environmental protections
Dilute with plenty of water.
Do not allow to enter sewers / surface or ground water.
Measures for cleaning / collecting
Absorb with liquid-binding material (sand, diatomite, acid binders, universal binders, sawdust).
Section 7- Handling and storage
Handling:
Information for safe handling: Avoid breathing dust, vapor, mist or gas. Avoid contact with eyes, skin and clothing.
Keep container tightly closed. Use with adequate ventilation.
Information about fire - and explosion protection: Not special measures required
Storage:
Requirement to be met by storerooms and receptables: Store in a cool, dry, well-ventilated area away from
incompatible substances. Keep away from metals.
Information about storage in one common storage facility: Not required
Further information about storage conditions: None
Storage class:
Class according to regulation on flammable liquids: void
Approved by / Approuv?par
Issue N? July,15 2002 S.Thomas / J. Wijdenes
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Material Safety Data Sheet
According to 91/155 EEC
Section 8 - Exposure Controls / Personal Protection
Additional information about design of technical facilities: No furhter data; see section 7
Ingredients with limit values that require monitoring at the workplace
The product does not contain any relevant quantities of material with critical values that have to be
monitored at the workplace.
Personal protective equipment
General protective and hygienic measures
The usual precautionary measures are to be adhered to when handling chemicals
Respiratory protection: Not required
Protection of hands: Protective gloves
Eye Protection: Goggles recommended during refilling
Section 9 - Physical and Chemical Properties
Form: Liquid
Colour: colourless
Odour: Odourless
Melting point/Melting range: N/A
Boiling point/Boiling range: N/A
Flash point: Not applicable
Autoignition temperature: N/A
Danger of explosion: Product does not present an explosion hazard
Densitiy: N/A
Solubility in / Miscibility with
water : fully miscible
Section 10 - Stability and Reactivity
Thermal decomposition: No decomposition if used according to specifications
Dangerous reaction: No dangerous reactions known
Dangerous decompositon products: No dangerous decomposition products known
Section 11 - Toxicological Information
Route of entry: Skin Contact, Eye contact, Inhalation, Ingestion
Effects of acute exposure to Product:
No irritant effect on the skin, eyes. No sensitizating effects known.
Additional toxicological information:
The product is not subject to classification according to the calculation method of the General EC Guidelines.
Exposure Limits Irritancy Sensitization To Product Carcinogenicity
N/A N/A N/A N/A
Terratogenicity Reproductive Toxicity Mutagenicity Synergistic Products
N/A N/A N/A N/A
Section 12 - Ecological Information
General Notes: Generally not hazardous for water
Section 13 - Disposal considerations
Waste disposal: dispose of in accordance with local official environmental regulations
Section 14 - Transport Information
Special shipping information: N/A
Section 15 - Regulations
Labelling according to European labelling guidelines:
Observe the general safety regulations when handling chemicals.
The product is not subject to identification regulations under EC Directives.
Approved by / Approuv?par
Issue N? July,15 2002 S.Thomas / J. Wijdenes
6
Material Safety Data Sheet
According to 91/155 EEC
Section 16 - Other information
This information is prepared on our present knowledge. However, this shall not constitute a guarantee for any specific
product features and shall not establish a legally valid contractual relationship.
Prepared by: Marketing Department Phone N?+33 3 81 41 38 38 Date: July 02
Approved by / Approuv?par
Issue N? July,15 2002 S.Thomas / J. Wijdenes
7
Material Safety Data Sheet
According to 91/155 EEC
Section 1 - Identification of substance
Product Identification: ELISA kit
Stop Reagent
Manufacturer: DIACLONE SAS
1, Bd Fleming
F-25000 Besan鏾n
France
Tel: +33 3 81 41 38 38
Fax: +33 3 81 41 36 36
e-mail: info@diaclone.com
www.diaclone.com
Section 2 - Data on components
Chemical characterization: Preparation
Hazardous ingredients: Concentration CAS Number EC Number
Sulfuric Acid 1N 7664-93-9 N/A
C ; R 35
Section 3 - Hazard Identification
Hazard Description: C Corrosive
Information concerning to particular hazards to man and environment: causes burns
Section 4 - First Aid Measures
General information Immediately remove any clothing soiled by the product.
After inhalation In case of unconsciousness place patient stably in side position for transportation.
After skin contact Immediately wash with plenty of water and soap and rinse thoroughly.
After eye contact Rinse opened eye for several minutes under running water. Then consult a physician.
After ingestion Do not induce vomiting; if conscious, give water, milk or milk magnesia.Call for a physician
immediately.
Section 5 - Fire Fighting Measures
Suitable extinguishing agents
Use dry chemical or carbon dioxyde. Do not use water.
Protective equipement No special measures required
Section 6 - Accidental Release Measures
Person-related safety precautions: Wear protective equipment. Keep unprotected persons away.
Measures for environmental protections
Prevent seepage into sewage system, workpits and cellars.
Dilute with plenty of water.
Do not allow to enter sewers/ surface or ground water.
Measures for cleaning / collecting
Absorb with liquid-binding material (sand, diatomite, acid binders, universal binders, sawdust).
Use neutralizing agent.
Dispose contaminated material as waste according to section 13.
Ensure adequate ventilation.
Section 7- Handling and storage
Handling:
Information for safe handling: Avoid contact with eyes, skin and clothing.Do not breath vapor. Keep container
tightly closed.
Information about fire - and explosion protection: No special measures required.
Approved by / Approuv?par
Issue N? July,15 2002 S.Thomas / J. Wijdenes
8
Material Safety Data Sheet
According to 91/155 EEC
Storage:
Storage requirement:
Keep in tightly closed container. Store separately and away from flammables and combustible materials.
Section 8 - Exposure Controls / Personal Protection
Additional information about design of technical facilities: No furhter data; see section 7
Ingredients with limit values that require monitoring at the workplace
The product does not contain any relevant quantities of material with critical values that have to be
monitored at the workplace.
Personal protective equipment
General protective and hygienic measures
Keep away from foodstuffs, beverages and feed.
Imediately remove all soiled and contaminated clothing.
Wash hands before breaks and at the end of work. Avoid contact with eyes and skin.
Respiratory protection: Not required
Protection of hands: Protective acid resistant gloves
Eye Protection: Tightly sealed goggles
Section 9 - Physical and Chemical Properties
Form: Liquid
Colour: Colourless
Odour: Odourless
Melting point/Melting range: N/A
Boiling point/Boiling range: N/A
Flash point: Not applicable
Autoignition temperature: N/A
Danger of explosion: Product does not present an explosion hazard
Densitiy: N/A
Solubility in / Miscibility with
water : fully miscible
Section 10 - Stability and Reactivity
Thermal decomposition: No decompsotion if used according to specifications
Reactivity and under what conditions:
A violent exothermic reaction occurs with water. Avoid contact with alkaline solutions, metals, metals powder,
chlorates, nitrates, strong oxiditing or reducing materials, and combustible organic materials.
Hazardous decompositon products (concentrated SULFURIC ACID):
Toxic fumes of oxides of sulfur when heated to decomposition. Will react with water or steam to produce toxic and
corrosive fumes. Reacts with carbonates to generate carbon dioxide gas, and with cyanides and sulfides to form
poisonous hydrogen cyanide and hydrogen sulfide respectively.
Section 11 - Toxicological Information
Route of entry: Skin Contact, Eye contact, Inhalation, Ingestion
Effects of acute exposure to Product:
Contact woith skin or eyes may cause severe irritation or burns. Vapors may be irritating to eyes, nose, and throat.
Poison may be fatal if swallowed, causes burns.
Effects of chronic exposure to Product:
Long term exposure to mist or vapors may cause damage to teeth. Chronic exposure to mists containing sulfuric acid
is a cancer hazard.
Exposure Limits Irritancy Sensitization To Product Carcinogenicity
N/A N/A N/A N/A
Terratogenicity Reproductive Toxicity Mutagenicity Synergistic Products
N/A N/A N/A N/A
Section 12 - Ecological Information
General Notes: hazardous for water
Do not allow product to reach ground water, water course or sewage system.
Must not reach sewage water or drainage ditch undiluted or uneutralized.
Danger to drinking water even if small quantities leak into the ground.
Approved by / Approuv?par
Issue N? July,15 2002 S.Thomas / J. Wijdenes
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Material Safety Data Sheet
According to 91/155 EEC
Section 13 - Disposal considerations
Waste disposal: dispose of in accordance with local official environmental regulations
Section 14 - Transport Information
Special shipping information:
Domestic (D.O.T) International (I.M.O)
Sulfuric Acid (with not more than 51% acid) Sulfuric Acid (with not more than 51% acid)
Proper Shipping Name:
Class 8 - UN2796 Class 8 - UN2796
Hazard Class:
Reportable quantity -
Packaging Group 200L - III 200L - III
Section 15 - Regulations
Labelling according to European labelling guidelines:
Observe the general safety regulations when handling chemicals.
The product has been classified and marked according EC Directives.
Code letter and hazard designation of product: C Corrosive
Section 16 - Other information
This information is prepared on our present knowledge. However, this shall not constitute a guarantee for any specific
product features and shall not establish a legally valid contractual relationship.
Prepared by: Marketing Department Phone N?+33 3 81 41 38 38 Date: July 02
Approved by / Approuv?par
Issue N? July,15 2002 S.Thomas / J. Wijdenes
10
Material Safety Data Sheet
According to 91/155 EEC
Section 1 - Identification of substance
Product Identification: ELISA kit
TMB (3,3',5,5'-Tetramethylbenzidine dihydrochloride hydrate)
Manufacturer: DIACLONE SAS
1, Bd Fleming
F-25000 Besan鏾n
France
Tel: +33 3 81 41 38 38
Fax: +33 3 81 41 36 36
e-mail: info@diaclone.com
www.diaclone.com
Section 2 - Data on components
Chemical characterization: Preparation
The following data is for the 100% chemical as no data is available on the solution.
Hazardous ingredients: % CAS Number EC Number
3,3',5,5'-Tetramethylbenzidine dihydrochloride 54827-17-7 259-364-6
T ; R 23/24/25
Section 3 - Hazard Identification
Hazard Description: not applicable
Information concerning to particular hazards to man and environment: Not applicable
Section 4 - First Aid Measures
After inhalation: Remove from exposure to fresh air immediately.Consult a physician in case of complaints.
After skin contact: Flush skin with plenty of soap and water for at least 15 minutes while removing contaminated
clothing and shoes.
After eye contact: Immediately flush eyes with plenty of water for at least 15 minutes, occasionally lifting the upper
and lower lid.
After ingestion Do not induce vomiting; allow the victim to rinse his mouth and then drink 2-4 cupfuls of water, and
seek medical advice.
Section 5 - Fire Fighting Measures
Suitable extinguishing agents
Use dry chemical, carbon dioxyde or water spray. Fight larger fires with water spray or alcohol resistant foam.
Protective equipement No special measures required
Section 6 - Accidental Release Measures
Person-related safety precautions: Not required.
Measures for environmental protections: No special measures required.
Measures for cleaning / collecting
Absorb with liquid-binding material (sand, diatomite, acid binders, universal binders, sawdust).
Section 7- Handling and storage
Handling:
Information for safe handling: Wash thoroughly after handling. Remove contaminated clothing and wash before
reuse. Avoid contact with eyes, skin and clothing. Avoid ingestion and inhalation.
Information about fire - and explosion protection: No special measures required.
Storage:
Storage requirement:
Store in a cool, dry place. Keep container closed when not in use. Refrigerate (~ 4癈).
Section 8 - Exposure Controls / Personal Protection
Additional information about design of technical facilities: No furhter data; see section 7
Ingredients with limit values that require monitoring at the workplace:
Approved by / Approuv?par
Issue N? July,15 2002 S.Thomas / J. Wijdenes
11
Material Safety Data Sheet
According to 91/155 EEC
The product does not contain any relevant quantities of material with critical values that have to be
monitored at the workplace.
Personal protective equipment
General protective and hygienic measures:
The usual precautionary measures are to be adhered to when handling chemicals.
Respiratory protection: Not required.
Protection of hands: Protective gloves.
Eye Protection: Goggles recommended during refilling.
Section 9 - Physical and Chemical Properties
Form: Liquid
Colour: Clear
Odour: Odourless
Melting point/Melting range: N/A
Boiling point/Boiling range: N/A
Flash point: Not applicable
Autoignition temperature: N/A
Danger of explosion: Product does not present an explosion hazard
Densitiy: N/A
Solubility in / Miscibility with
water : fully miscible
Section 10 - Stability and Reactivity
Thermal decomposition: No decompsotion if used according to specifications
Reactivity and under what conditions:
Incompatibles with bases, amines, alkali metals, copper, copper alloys
Hazardous decompositon products
Toxic fumes of oxides of carbon monoxyde, carbon dioxyde, nitrous oxydes, hydrogen chloride gas, hydrogen
bromide gas, phosphorous oxydes.
Section 11 - Toxicological Information
Route of entry: Skin Contact, Eye contact, Inhalation, Ingestion
Effects of acute exposure to Product:
Contact with skin or eyes may cause severe irritation or burns. Vapors may be irritating to eyes, nose, and throat.
Effects of chronic exposure to Product: N/A
Exposure Limits Irritancy Sensitization To Product Carcinogenicity
N/A N/A N/A N/A
Terratogenicity Reproductive Toxicity Mutagenicity Synergistic Products
N/A N/A N/A N/A
Section 12 - Ecological Information
General Notes: N/A
Section 13 - Disposal considerations
Waste disposal: dispose of in accordance with local official environmental regulations
Section 14 - Transport Information
Special shipping information:
Does not need to be shipped as hazardous.
Section 15 - Regulations
Labelling according to European labelling guidelines:
Observe the general safety regulations when handling chemicals.
Section 16 - Other information
This information is prepared on our present knowledge. However, this shall not constitute a guarantee for any specific
product features and shall not establish a legally valid contractual relationship.
Prepared by: Marketing Department Phone N?+33 3 81 41 38 38 Date: July 02
Approved by / Approuv?par
Issue N? July,15 2002 S.Thomas / J. Wijdenes
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