MATERIALS SAFETY DATA SHEET MSDS/BIO81DNA
ISSUE: 2
1. Identification of the substance/preparation and of the company/undertaking:
Identification of the substance or preparation:
OxyDNA Test. Cat No.: BIO81DNA
Use of the substance/preparation: Biotrin OxyDNA Test is an in-vitro fluorescent protein
binding method for the detection of oxidative damage to DNA in fixed permeabilised cells.
Company/Undertaking Identification: Biotrin International,
93 The Rise,
Mount Merrion,
Co. Dublin,
Ireland.
www.biotrin.com
Telephone: 00 353 1 283 11 66 Fax: 00 353 1 283 12 32
Emergency Telephone: 00 353 1 283 11 66 E-mail: info@biotrin.ie
2. Composition/Information on Ingredients:
Wash Concentrate: 25x TRIS buffered aline/Tween-20 (contains 0.01% thiomersal)
FITC Conjugate: 0.5ml Binding protein-FITC conjugate (contains 0.01%
Thiomersal)
Blocking Solution: 5ml (10x) solution (contains 15mM sodium azide). Contains
0.1% Sodium Azide (Xn - Harmful) risk phrases (R22, R32)
鈥淗armful if swallowed and contact with acids liberates very toxic gas.鈥?
3. Hazards Identification:
Sodium Azide is an irritant. Avoid contact with components containing azide. Do not ingest or
inhale. In the concentrations present in the blocking solution , sodium azide is classified as Harmful
and subject to the following risk phrases (R22, R32) 鈥淗armful if swallowed and contact with acids
liberates very toxic gas.鈥?
Thiomersal is toxic by inhalation, in contact with skin and if swallowed. It is irritating to eyes,
respiratory system and skin. It is a possible mutagen, which may affect kidneys and nerves.
However Thiomersal is not considered hazardous in the concentrations present in kit solutions.
Trizma base is irritating to eyes respiratory system and skin. However Trizma base is not
considered hazardous in the concentrations present in kit solutions.
4. First Aid Measures: Applicable for all kit components
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Date of Issue: January 2005
MATERIALS SAFETY DATA SHEET MSDS/BIO81DNA
ISSUE: 2
Inhalation: If exposure is severe remove to fresh air.
Skin: Wash skin with copious amounts of water. Remove contaminated
clothing and shoes.
Eye: Flush eyes with copious amounts of water for at least 15 minutes. Assure
adequate flushing of the eyes by separating the eyelids. If any irritation
persists, obtain medical assistance.
Ingestion: Wash out mouth with copious amounts of water. Give plenty of water to
drink. Obtain medical assistance if large quantity is ingested.
5. Fire-fighting measures:
Precautions during fire: Avoid inhalation of fumes
Suitable extinguishing media: Use media suitable to extinguish supporting or surrounding fire.
Do not use water on sodium azide containing components.
Special exposure hazards: Some components may decompose and emit toxic fumes under
fire conditions (Sodium Azide, Trizma base).
Special fire-fighting procedures: Self-contained breathing apparatus may be required if heavy
fumes are emitted. It is advisable for protective clothing to be
worn to prevent contact with skin and eyes.
6. Dispositions after accidental spilling:
N/A
7. Accidental release measures:
Personal Precautions : Wear Protective clothing for the prevention of skin and eye
contact.
Environmental precautions: Dispose of any contaminated material in biohazardous disposal
units and in accordance with established safety procedures.
Methods for cleaning up: Wipe up spills with absorbent paper, then clean area with a
concentrated chlorine solution. Discard all materials used to wipe
up spills using biohazard waste facilities. Residues of extract
solution are generally considered as potentially hazardous waste.
All such materials should be disposed of in accordance with
established safety procedures.
8. Handling and storage:
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Date of Issue: January 2005
MATERIALS SAFETY DATA SHEET MSDS/BIO81DNA
ISSUE: 2
Handling: All clinical specimens and any infected or potentially infected
material should be handled as though potentially infectious.
Store at 2 - 8oC
Storage:
Specific Use: This product is for laboratory use only.
9. Exposure controls/personal protection:
All patient specimens are considered potentially biohazardous materials. They should be handled
at the Biosafety Level 2 as recommended for any potentially infectious specimen in the
CDC/NIH manual 鈥淏iosafety in Microbiological and Biomedical Laboratories鈥?, 1998.
Some of the reagents in this kit contain toxic or irritant components (Refer to section 3). Wear
protective clothing, disposable latex gloves and eye protection while handling specimens and
performing the assay. Wash hands thoroughly when finished.
10. Physical and chemical properties:
Not Applicable
11. Stability and reactivity:
OxyDNA Test kit is stable at 2 - 8oC.
The Blocking Solution contains Sodium azide at a concentration of 0.1 %, which may form
potentially explosive metal azides with lead and copper plumbing. Sodium azide explodes when
heated and may form hazardous combustion or decomposition products.
12. Toxicological information:
Blocking Solution contains Thiomersal and Sodium azide which are toxic by inhalation, in
contact with skin and if swallowed.
13. Ecological information:
No known ecological hazards
14. Disposal considerations:
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MATERIALS SAFETY DATA SHEET MSDS/BIO81DNA
ISSUE: 2
Residues of test materials are generally considered as hazardous waste. All such materials should
be disposed of in accordance with established safety procedures.
15. Transport information:
No specific recommendations
16. Regulatory information:
Directive 2001/58/EC
17. Other information:
Training : This product should be handled only by technically qualified individuals.
The contents of this MSDS should be known before use.
Restrictions on Use: This product is for laboratory use only.
Signed: ____________________ Date: _________14/01/05_______________
Approved: _____________________ Approved: ______________________
Quality Systems Manager Health and Safety Officer
Date: ______14/01/05___________________ Date: ______14/01/05_________________
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Date of Issue: January 2005
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