Date revised: 10/20/2009
MATERIAL SAFETY DATA SHEET
Conforms to 93/112/EC and ISO 11014-1
1. Chemical Product and Company Identification
Product Name: Triple Dye Loading Buffer (6X) Product Number: EC-855
Chemical Names/
Aqeuous solution of sucrose, tris base, and dyes.
Description:
Manufacturer Telephone Numbers
National Diagnostics
(800) 526-3867
305 Patton Drive
(404) 699-2121
Atlanta, GA 30338
Emergency Number
Chemtrec
(800) 424-9300
2. Composition/Information on Ingredients
Component % Comp. CAS # EINECS # TLV (Units)
Sucrose 50 57-50-1 10 mg/m3
Xylene Cyanol 2650-17-1 224-728-5 none established
Orange G 1936-15-8 none established
Bromophenol Blue 115-39-9 204-086-2 none established
Tris-Base 77-86-1 201-064-4 none established
3. Hazards Identification
Appearance and Odor
dark blue, odorless liquid
EMERGENCY OVERVIEW - IMMEDIATE HAZARD
HIGH CONCENTRATIONS MAY IRRITATE EYES AND RESPIRATORY TRACT.
EMERGENCY OVERVIEW - CHRONIC HAZARD WARNING:
AS PART OF GOOD LABORATORY AND PERSONAL HYGIENE AND SAFETY
PROCEDURE, AVOID ALL UNNECESSARY EXPOSURE TO THE CHEMICAL
SUBSTANCE AND ENSURE PROMPT REMOVAL FROM SKIN, EYES, AND CLOTHING.
Potential Health Effects
Inhalation
Not expected to be a health hazard. In dry form, sucrose is a nuisance dust. Inhalation of high
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concentration may cause upper respiratory tract irritation.
Ingestion
Extremely large oral doses may produce gastrointestinal disturbances.
Skin
No adverse effects expected.
Eyes
No adverse effects expected but in dry form, dust may cause mechanical irritation.
Signs and Symptoms of Overexposure
Inhalation
Coughing.
Ingestion
Nausea and stomach upset possible with extremely large oral doses.
Skin
No adverse effects expected.
Eyes
Redness and itching with mechanical irritation.
Carcinogenicity
Not listed as a known or anticipated carcinogen by NTP or IARC.
Mutagenicity
No information found.
Reproductive Toxicitiy
No information found.
Teratogenic Effects
No information found.
Routes of Entry
No information found.
Target Organ Statement
No information found.
4. First Aid Measures
Inhalation
Remove to fresh air. If not breathing, give artificial respiration. If breathing is difficult, give oxygen. Call
a physician.
Ingestion
Induce vomiting immediately as directed by medical personnel. Never give anything by mouth to an
unconscious person. Call a physician.
Skin
Immediately flush skin with plenty of soap and water for at least 15 minutes while removing
contaminated clothing and shoes. Get medical attention. Wash clothing before reuse. Thoroughly clean
shoes before reuse.
Eyes
Immediately flush eyes with plenty of water for at least fifteen minutes, lifting lower and upper eyelids
occasionally. Get medical attention immediately.
5. Fire Fighting Measures
Flash Point N.A. Flammable Limits N.A.
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Flash Point Method N.A. Autoignition N.A.
temperature
Extinguishing media
Use media appropriate to the primary cause of fire.
Protective Equipment
In the event of a fire, wear full protective clothing and NIOSH-approved self-contained breathing
apparatus with full facepiece operated in the pressure demand or other positive pressure mode.
Hazardous Combustion Products
Thermal decomposition products may include toxic oxides of nitrogen and carbon.
Unusual Fire and Explosion Hazards
Not considered an explosion hazard.
NFPA Codes: Health 1 Flammability 0 Reactivity 0
6. Accidential Release Measures
Steps to be taken in case material is released or spilled
Contain and clean up spill immediately, prevent from entering floor drains. Contain liquids using
absorbents. Shovel all spill materials into disposal drum. Scrub spill area with detergent, flush with
copious amounts of water.
Waste Disposal Method
Disposal must be made in accordance with applicable federal, state, and local regulations.
Personal Precautions
Wear appropriate protective equipment as specified in section 8.
7. Handling and Storage
Handling
Avoid contact and inhalation. Do not get in eyes, on skin, on clothing. Wash thoroughly after handling.
Storage
Keep in a tightly closed container, stored in a cooled, dry, ventilated area.
Storage Temperature
4C
Disposal
Observe all national, state, and local regulations regarding disposal.
8. Exposure Controls/Personal Protection
Airborne Exposure Limits
Component: Sucrose
ACGIH Threshold Limit Value (TLV): 10 mg/m3
OSHA Permissable Exposure Limit
15 mg/m3 total dust, 5 mg/m3 respirable fraction
(PEL):
Engineering Controls
A system of local and/or general exhaust is recommended to keep employee exposures below the
Airborn Exposure Limits. Local exhaust ventilation is generally preferred because it can control the
emissions of the contaminant at its source.
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Respiratory Protection
For conditions of use where exposure to the dust or mist is apparent, a full-face dust/mist respirator
may be worn. For emergencies or instances where the exposure levels are not known, use a full-face
positive-pressure, air-supplied respirator.
Eye Protection
Use chemical safety goggles and/or a full face shield where splashing is possible. Maintain eye wash
fountain and quick-drench facilities in work area.
Skin Protection
Wear protective gloves and clean body covering clothing.
Other Control Measures
N.A.
9. Physical Properties
Boiling point 220 F Evaporation Rate 1.0
Melting point N.A. Solubity in water Soluble
Vapor pressure (mmHg) Water pH 8.3
Vapor density (Air = 1) N.A. Specific gravity (H2O = 1) 1.16
% volatile by volume 31
10. Stability and Reactivity
Stability
Stable under ordinary conditions of use and storage.
Conditions to Avoid
Heat, incompatibles.
Hazardous Decomposition Products
Thermal decomposition can produce carbon monoxide and carbon dioxide.
Hazardous Polymerization
Will not occur
Incompatibles
Sucrose:
Oxidizers, sulfuric acid and nitric acid.
Xylene Cyanol:
Strong oxidizing agents, strong reducing agents.
Orange G:
No information found.
Bromophenol Blue:
Strong oxidizers.
Tris-Base:
No incompatibility data found.
11. Toxicological Information
Product LD50 Values
Triple Dye Loading Buffer
Oral Rat LD50 (mg/kg): 59400
(6X)
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Triple Dye Loading Buffer
Dermal Rabbit LD50 (mg/kg): N.A.
(6X)
Component Cancer List Status
NTP Carcinogen
IARC
Known Anticipated
Category
Sucrose No No None
Xylene Cyanol No No None
Orange G No No None
Bromophenol Blue No No None
Tris-Base No No None
12. Ecological Information
Sucrose
No information found.
Xylene Cyanol
No information found.
Orange G
No information found.
Bromophenol Blue
No information found.
Tris-Base
No information found on either the environmental fate or environmental toxicity of this material.
13. Disposal Considerations
Observe all national, state, and local regulations regarding disposal.
14. Transport Information
D.O.T.
Proper Shipping Name: Not Regulated
Hazard Class: N.A.
UN Number: N.A.
Packing Group: N.A.
I.A.T.A.
Proper Shipping Name: Not Regulated
Hazard Class: N.A.
UN Number: N.A.
Packing Group: N.A.
I.M.O.
Proper Shipping Name: Not Regulated
Hazard Class: N.A.
UN Number: N.A.
Packing Group: N.A.
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15. Regulatory Information
United States
TSCA Regulatory Statement
All intentional ingredients are listed on the TSCA Inventory.
SARA 311/312 Hazard Categories
Component Fire Pressure Reactivity Acute Chronic
Sucrose Yes No No No No
Xylene Cyanol No No No Yes Yes
Orange G No No No No No
Bromophenol Blue No No No Yes No
Tris-Base No No No Yes No
Europe
EEC Regulatory
All intentional ingredients are listed on the European EINECS Inventory.
16. Other Information
NFPA Codes: Health 1 Flammability 0 Reactivity 0
MANUFACTURER DISCLAIMER: The information given herein is offered in good faith as accurate, but without
guarantee. Conditions of the use and suitability of the product for particular uses are beyond our control. All
risks of use of the product are therefore assumed by the user. Nothing is intended as a recommendation for
uses which infringe valid patents or as extending license under valid patents. Appropriate warnings and safe
handling procedures should be provided to handlers and users.
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