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MSDS Material Safety Data Sheet
CAS

56-81-5 9005-64-5 127087-87-0 2650-17-1 1936-15-8

File Name: 56-81-5_9005-64-5_127087-87-0_2650-17-1_1936-15-8.asp

                                             MATERIAL SAFETY DATA SHEET
Telephone: (978)927-5054
Polymerase
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New England Biolabs
e-mail: info@neb.com
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Ipswich, MA 01938
Msds Revision Date: 10/06




SECTION 1瑿HEMICAL INFORMATION

Product Name: Quick Load Taq 2X Master Mix SARA 313 NO

SECTION 2瑿OMPOSITION/INFORMATION ON INGREDIENT

NAME OF CHEMICALS:
1. Glycerol 10 % Cas. #56-81-5 NO
2. Tween-20 < 1% Cas. #9005-64-5 NO
3. NP-40 < 1% Cas. #127087-87-0 NO
4. xylene cyanol FF < 1% Cas. #2650-17-1 NO
5. orange G < 1% Cas. #1936-15-8 NO


SECTION 3璈AZARDOUS IDENTIFICATION
EMERGENCY OVERVIEW
Caution: Avoid contact and inhalation.
HMIS RATING
HEALTH: 2
FLAMMABILITY: 0
REACTIVITY: 0
NFPA RATING
HEALTH: 2
FLAMMABILITY: 0
REACTIVITY: 0
For additional information on toxicity, please refer to Section 11.

SECTION 4璅IRST AID MEASURES
ORAL EXPOSURE: If swallowed, wash out mouth with water provided person is conscious. Call a physician.
INHALATION EXPOSURE: If inhaled, remove to fresh air.If breathing becomes difficult, call a physician.
DERMAL EXPOSURE: In case of contact, immediately wash skin with soap and copious amounts of water. Remove clothing and call a
physician.
EYE EXPOSURE: In case of contact, immediately flush eyes with copious amounts of water for at least 15 minutes. Assure adequate
flushing by separating the eyelids with fingers. Call a physician.


SECTION 5璅IRE FIGHTING MEASURES
Extinguishing Media: Suitable: Water spray.
Carbon Dioxide, dry chemical powder or appropriate foam.
Special Firefighting Procedures: Wear self contained breathing apparatus and protective clothing to prevent contact with skin and eyes.
Specific Hazard(s): Emits toxic fumes under fire conditions.

Flash point: N/A
Flammability: N/A
Autoignition Temp: N/A



Page 1
SECTION 6瑼CCIDENTAL RELEASE MEASURES
PROCEDURE(S) OF PERSONAL PRECAUTION(S): Wear self-contained breathing apparatus, rubber boots and heavy rubber
gloves and Chemical safety googles.
METHODS FOR CLEANING UP: Cover with dry lime, sand, or soda ash. Place in covered containers using non-sparking tools and
transport outdoors. Ventilate area and wash spill site after material pickup is complete.



SECTION 7璈ANDLING AND STORAGE
Handling: User Exposure: Avoid inhalation. Avoid contact with eyes, skin and clothing. Avoid prolonged or repeated exposure.
Storage: Suitable: Keep tightly closed.

SECTION 8璄XPOSURE CONTROLS/PPE
Engineering Controls: Safety shower and eye bath. Mechanical exhaust required.
Personal Protective Equipment: Goverment approved respirator.
Hand: Compatible chemical-resistant gloves.
Eye: Chemical safety goggles.
General Hygiene Measures: Wash hands thoroughly after handling. Wash contaminated clothing before use.


SECTION 9璓HYSICAL AND CHEMICAL PROPERTIES

Physical Properties:
Melting Point: 20癈
Boiling Point: 182癈
Flash Point: 320癋, 160癈 Method: closed up
Autoignition Temp: 370癈

Explosion Limits in Air:
Lower: 0.9%
Specific Gravity: 1.262
Solubility: Water -Z26130
Vapor Pressure: < 1 MMHG @ 20癈
Vapor Density: 3.1 G/L
PH: 5.5?.0


SECTION 10璖TABILITY AND REACTIVITY

Stability: Stable
Materials to Avoid: Strong oxidizing agents.
Hazardous Decomposition Products: Carbon monoxide, carbon dioxide, sulfur dioxides.
Hazardous Polymerization: Hazardous Polymerization: Will not occur


SECTION 11璗OXICOLOGICAL INFORMATION

Route of exposure:
Skin contact: May cause skin irritaiton.
Multiple Routes: May be harmful by inhalation, ingestion, or skin absorption.
Eye contact: May cause eye irritation.
Inhalation: May be harmful if inhaled. Material may be irritating to mucous membranes and upper respiratory tract.
Signs and Symptoms of Exposure: Nausea, headache and vomiting. To the best of our knowledge, the properties
have not been thoroughly investigated.

IRRITATION DATA:
SKN-RBT 5OO MG/24H MLD 85JCAE -, 207, 1986
EYE-RBT 126 MG MLD BIOFX* 9-4/970
EYE-RBT 5OO MG/24H MLD 85JCAE -, 207, 1986




Page 2
TOXICITY DATA:
ORL-RAT LD5O: 12600 MG/KG FEPRA7 4, 142, 1945
IHL-RAT LC5O: > 570 MG/M3/1H BIOFX* 9-4/970
IPR-RAT LD5O: 4420 MG/KG RCOCB8 56, 125,1987
SCU-RAT LD5O: 100 MG/KG NIIRDN 6, 215, 1982
IVN-RAT LD5O: 5566 MG/KG ARZNAD 26,1581,1976
ORL-MUS LD50: 4090 MG/KG FRZKAP 6), 56, 1977
IPR-MUS LD50: 8700 MG/KG ARZNAD 28,1579,1978
SCU-MUS LD5O: 91 MG/KG NIIRDN 6, 215, 1982
IVN-MUS LD5O: 4250 MG/KG JAPMA8 39, 583, 1950
ORL-RBT LD5O: 27 GM/KG DMDJAP 31, 276,1959
SKN-RBT LD5O: >10 GM/KG BIOFX* 9-4/970
IVN-RBT LD5O: 53 GM/KG NIIRDN 6, 215, 1982
ORL-GPG LD5O: 7750 MG/KG JIHTAB 23, 259, 1941


TARGET ORGAN DATA:
Behavioral (headache)
Gastrointestinal (nausea or vomiting)
Kidney, ureter, bladder (changes in tubules)
Kidney, ureter, bladder (changes in urine comp)
Paternal effects (spermatogenesis)
Paternal effects (testes, epididymis, sperm duct)
Effects on fertility (male ferility index)
Effects on fertility (post-implantation mortality
Only selected registry of toxic effects of chemical substance
(RTECS) data is presented here. See actual entry in RTECS


SECTION 12璄COLOGICAL INFORMATION
No Data Available.


SECTION 13璂ISPOSAL CONSIDERATIONS
Contact a licensed professional waste disposal service to dispose of this material. This combustible material may be burned in a chemi-
cal incinerator equipped with an afterburner and scrubber. Observe all federal, state and local environmental regulations.


SECTION 14璗RANSPORT INFORMATION
DOT
Proper Shipping Name: None
Non-Hazardous for Air Transport: non-hazardous for air transport.

IATA
Non-Hazardous for Air Transport: non-hazardous for air transport.

SECTION 15璕EGULATORY INFORMATION
US Classification and Label Text
US Statements: Avoid contact and inhalation.
United States Regulatory Information:
Sara Listed: No
Canada Regulatory Information WHMIS Classification: This product has been classified in accordance with the hazard criteria of the CPR
and the MSDS contains all the information required by the CPR.
DSL: Yes
NDSL: No


SECTION 16璒THER INFORMATION
DISCLAIMER
For R&D use only. Not for drug, household or other uses.
The above information is believed to be correct but does not purport to be all inclusive and shall be used only as a guide.
New England Biolabs shall not be held liable for any damage resulting from handling or from contact with the above product.

Page 3

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