FP09 I21 R02A.3
Date: 21/09/2009
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MATERIAL SAFETY DATA SHEET
FertiPro N.V.- Belgium
1. CHEMICAL PRODUCT AND COMPANY IDENTIFICATION
Product name : Spermac stain
Product use : Stain for morphology evaluation of spermatozoa
Manufacturer : FertiPro N.V.
Distributor : FertiPro N.V.
Industriepark Noord 32
B-8730 Beernem
Belgium
For emergency information : 32 50 79 18 05
E-mail : info@fertipro.com
2. COMPOSITION / INFORMATION ON THE INGREDIENTS
Product formal name: diagnostic reagent
Product components: Component CAS-code Approx. %
Spermac Stain Fix
Formalin (fix) 50-00-0 4%*
Water 7732-18-5 96%
Spermac Stain A
Ethyl alcohol 64-17-5 < 10%
Rose Bengal 632-69-9 < 1%
Neutral Red 553-24-2 < 1%
Water 7732-18-5 Remaining
Spermac Stain B
Ethyl alcohol 64-17-5 < 10%
Pyronin Y 92-32-0 < 1%
Orange G 1936-15-8 < 1%
Phophomolybdic acid 51429-74-4 < 1%
Water 7732-18-5 Remaining
Spermac Stain C
Janus Green 2869-83-2 < 1%
Fast Green FCF 2353-45-9 < 1%
Water 7732-18-5 Remaining
3. HAZARD IDENTIFICATION
Ethyl alcohol: flammable.
Formalin: Toxic by inhalation, in contact with skin and if swallowed. Causes burns. Possible risk of
irreversible effects. May cause sensitisation by skin contact. Canc. Cat 3.
Phosphomolibdic acid: Contact with combustible material may cause fire. Causes burns.
Pyronin Y: Harmful by inhalation, in contact with skin and if swallowed. Possible risk of irreversible effects.
Orange G: Irritating to eyes, respiratory system and skin.
Fast Green FCF: Harmful by inhalation, in contact with skin and if swallowed. Possible risk of irreversible
effects.
4. FIRST AID MEASURES
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Inhalation : remove to fresh air, If not breathing give artificial
respiration. Call a physician
Eyes : flush with copious amounts of water for at least 15
minutes. Assure adequate flushing by separating the
eyelids with fingers
Skin : flush with copious amounts of water for at least 15
minutes, stubborn stains may be removed with repeated
alcohol swaps followed by soap and water. Remove
contaminated clothing and shoes
Ingestion : wash out mouth with water provided person is conscious.
Call a physician immediately. Do not induce vomiting.
5. FIRE FIGHTING MEASURES
Fire hazard : flammable at temperatures above 100? C.
Extinguishing media : water spray, carbon dioxide, dry chemical powder or
appropriate foam.
Special fire fighting procedure : wear self contained breathing apparatus and protective
clothing to prevent contact with skin and eyes. Toxic
fumes may be emitted under fire conditions.
Unusual fire and explosion hazards : not established.
6. ACCIDENTAL RELEASE MEASURES
Rinse spill area with water, wash contaminated clothing before reuse.
7. HANDLING AND STORAGE
Store at room temperature (15-25?Avoid direct contact with eyes, skin or clothing. Do not breath vapour.
C).
Avoid prolonged or repeated exposure.
8. EXPOSURE CONTROL / PERSONAL PROTECTION
The use of rubber gloves and safety goggles is indicated when working with the reagents in the laboratory.
Avoid contact and inhalation, wash hands thoroughly after handling. Wash contaminated clothing before
reuse.
9. PHYSICAL AND CHEMICAL PROPERTIES
Appearance: clear liquid (fixative)
red liquid (Stain )
yellow liquid (Stain B)
green liquid (Stain C)
Solubility in water: complete
10. STABILITY AND REACTIVITY
Stability : stable
Incompatibility : strong oxidizing agents
Hazardous decomposition products : carbon monoxide, carbon dioxide
11. TOXICOLOGICAL INFORMATION
Formalin : harmful if swallowed, inhaled or absorbed through skin.
Irritant to mucous membranes. Symptoms of exposure
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may include burning sensation, coughing, wheezing,
laryngitis, shortness of breath, headache, nausea and
vomiting. Inhalation may result in spasm, inflammation
and edema of the larynx and bronchi, chemical
pneumonitis, and pulmonary edema. Coughing, chest
pains, difficulty in breathing. Gastrointestinal
disturbances. May cause convulsions. Listed as
carcinogen by NTP, IARC, OSHA, ACGIH and EPA. To
the best of our knowledge, the chemical, physical and
toxicological properties have not been thoroughly
investigated.
Target organs: Eyes, kidneys, liver and heart.
Phophomoliybdic acid : harmful if swallowed, inhaled or absorbed through skin.
Irritant to mucous membranes. To the best of our
knowledge, the chemical, physical and toxicological
properties have not been thoroughly investigated.
Rose Bengal : may be harmful if swallowed, inhaled or absorbed through
skin. May be irritant to mucous membranes. To the best
of our knowledge, the chemical, physical and toxicological
properties have not been thoroughly investigated
Neutral Red : may be harmful if swallowed, inhaled or absorbed through
skin. May be irritant to mucous membranes. To the best
of our knowledge, the chemical, physical and toxicological
properties have not been thoroughly investigated
Pyronin Y : may be harmful if swallowed, inhaled or absorbed through
skin. May be irritant to mucous membranes. To the best
of our knowledge, the chemical, physical and toxicological
properties have not been thoroughly investigated
Orange G : may be harmful if swallowed, inhaled or absorbed through
skin. May be irritant to mucous membranes. To the best
of our knowledge, the chemical, physical and toxicological
properties have not been thoroughly investigated
Janus Green : may be harmful if swallowed, inhaled or absorbed through
skin. May be irritant to mucous membranes. To the best
of our knowledge, the chemical, physical and toxicological
properties have not been thoroughly investigated
Fast Green : may be harmful if swallowed, inhaled or absorbed through
skin. May be irritant to mucous membranes. To the best
of our knowledge, the chemical, physical and toxicological
properties have not been thoroughly investigated
Ethyl alcohol : potential fire hazard
12. ECOLOGICAL INFORMATION
No data available.
13. DISPOSAL
Rinse spill area with water, wash contaminated clothing before reuse.
Fixative
Contact a licensed professional waste disposal service to dispose of this material. Dissolve or mix the
material with a combustible solvent and burn in a chemical incinerator equipped with an afterburner and
scrubber. Observe all local environmental regulations.
14. TRANSPORT INFORMATION
Not classified as Hazardous for Transport.
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15. REGULATORY INFORMATION
Fixative
Identification of danger: Xn ?Harmful
R-phrases: 40-43: Limited evidence of carcinogenic effect. May cause sensitization by skin contact.
S-phrases: 36/37: Wear suitable protective clothing and gloves.
Stain A and B
R-Phrases: 10: Flammable
16. OTHER INFORMATIONS
The information contained herein is furnished without warranty of any kind. Users should consider these data
only as a supplement to other information gathered by them and must take independent determinations of the
suitability and completeness of information from all other sources to assure proper use and disposal of these
materials and the safety and health of employees and customers.
Nor Stain Enterprises Inc., nor Dr. EE Oettl? nor FertiPro N.V. shall be held liable for any damage resulting
from handling or from contact with its products.
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