MSDS8878







                               T N te E ina n C., Inc

                                he itra lim tio o    .

                                      334 Hecla S Lake Linden MI 49945

                                                   t.,

                                     T 906/296-1000 Fax: 906/296-8003

                                      el:

                                 ellenr@nitrate.c om   Web: www.nitrate.com

Nitrate Test Kits: Agricultural Field Kit Reagent System, all Kit Formats

The active, nitrate reducing reagents are the enzyme nitrate reductase and the biological electron donor molecule,

NADH (E-Nicotinamide adenine dinucleotide, reduced disodium salt hydrate).

Assay Buffer: 28 mM Potassium Phosphate @ pH 7.5, 0.025 mM EDTA (Ethylenediaminetetraacetic acid).

Color Reagent: Citric Acid and Griess Reagents (less than 1 mg each of Sulfanilamide and N-(1-

Napthyl)ethylenediamine Hydrochloride).



None of these reagents or solutions is classed as hazardous. No UN/NA codes.

No applicable regulations for international transportation. No labels required.



We provide a full MSDS for the Nitrate Reductase Enzyme. MSDS for all chemicals included in the kits are available upon request.

         Chemical Name                     CAS Number               Chemical Formula                Quantity per Liter

                                          9013-03-0             Complex Peptide                     <1 microgram

 Nitrate Reductase

 NADH (E-Nicotinamide

 adenine dinucleotide, reduced

 disodium salt hydrate)                   606-68-8              C21H27N7Na2O14P2                    <1 milligram

 Potassium Phosphate,

                                          7778-77-0             KH2PO4                              <1 gram

 Monobasic

 EDTA

 (Ethylenediaminetetraacetic

 acid disodium salt dihydrate)            6381-92-6             C10H14N2O8.2Na.2H2O                 <1 milligram

                                          77-92-9               C6H8O7                              ~5 grams

 Citric Acid

                                          63-74-1               C6H8N2O2S                           <10 milligrams

 Sulfanilamide

 NED (N-(1-

 Napthyl)ethylenediamine

 Hydrochloride)                           1465-25-4             C12H14N2                            <1 milligram



Section 1: Chemical Identification

Common name, Chemical name, CAS number, Formula: See Table Above



Section 2: Composition

Chemical and Common names: see Table Above

Principal Hazardous Component: NA     Biologic Activity: NA

Exposure Limits: Not established



Section 3: Hazards Identification

Threshold Limit Value: varied, but below known limits as supplied in kits



Section 4: First Aid Measures

Enzymes/Proteins may cause allergic reactions in certain sensitive individuals. Any chemical may cause skin or eye

irritation. Avoid contact with Color Reagent.

Inhalation: Remove affected individual from source, then seek medical attention.

Eyes: Remove contact lenses if present. Flush thoroughly with saline or water. Contact physician if irritation or

redness persists.

Skin: Wash material from clothing or skin using soap and water. Contact physician if irritation develops. Clean

clothing before reuse.

Ingestion: May be harmful if swallowed. Obtain medical attention as required.







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Section 5: Fire Fighting Measures

No special fire fighting equipment, media, or clothing is required.

Auto-ignition temperature: N/A Flash Point: N/A                 Flammable Limits in Air (% by volume): N/A

Unusual fire or Explosion Hazards: N/A Stability: Degradation products are not hazardous

Section 6: Accidental Release Measures

Wear appropriate protective equipment for storage and when handling. No additional precautions are required.

If Released or Spilled: Wear approved goggles and protective gloves. Collect spilled powders or solids into waste

container for disposal. Avoid physical contact during removal, especially any dust. Use normal cleanup procedures

for liquid spills. Wash area thoroughly with water. Wash contaminated clothing prior to reuse.

Waste Disposal: Dispose of waste in accordance with all applicable Federal, State, and Local regulations.

Section 7: Handling and Storage

Refer to product label for storage conditions. Store lyophilize and freeze-dried proteins in a cool, dry area in tightly

closed containers when not in use. Some proteins are temperature-sensitive: store at temperature suggested on label.

Handling: Wear appropriate protective equipment; see Section 8. Avoid generation of dust or aerosols.

Section 8: Exposure Controls and Personal Protection

Respiratory Protection: (approved dust mask or respirator) is advisable when handling a powdered form of a

product in order to avoid breathing particulates. Wear protective gloves and goggles or safety glasses to prevent

direct contact with skin or eyes. Sensitive individuals should wear protective clothing.

Ventilation: Advisable, standard vent fan and local exhaust as required.

Section 9: Physical and Chemical Properties

Appearance and Odor: Powders, white

Solubility in Water: Soluble in water

Section 10: Stability and Reactivity

Stability: Stable        Hazardous Decomposition Products: None Known                       Hazardous

Polymerization: Will Not Occur

Section 11: Toxicological Information

Acute Overexposure: May cause skin, eye, or respiratory irritation upon contact

Chronic Overexposure: May cause allergic reactions in certain sensitive individuals

The toxicological properties of this material have not been fully investigated. Always follow Good Laboratory and

Industrial Hygiene Practices and wear personal protective equipment when handling chemicals.

Section 12: Ecological Information

Environmental Hazard: None Known

Section 13: Disposal Considerations

Dispose of waste in accordance with all Local, State, and Federal regulations. Chemical residues are generally

classified as special waste: therefore transportation, storage, treatment, and disposal must be conducted in

compliance with all applicable regulations. Rinse empty containers thoroughly before recycling.

Section 14: Transport Information

Proper Shipping Name: Not regulated     Hazard Class: N/A                Packaging Group: N/A

Reportable Quantity: N/A        Labels Required: N/A

International Transportation Requirements: Not Regulated                          UN/NA Code: N/A

Section 15: Regulatory Information

US Federal and State: Materials are exempt for the US Environmental Protection Agency Toxic Controlled

Substances Control Act (TSCA) inventory. Not known to be listed or regulated under any State jurisdiction.

International: EC: /R42/43, May cause sensitization by inhalation and skin contact. S36, wear suitable, protective

clothing.

Harmonized shipping code: Lab Reagents 3822.00.20



Section 16: Other Useful Information

The information provided in this MSDS is believed to be correct but does not purport to be all inclusive. The

information is presented as a guide. NECi assumes no liability for its accuracy or completeness. NECi does not

assume liability for damages resulting from handling, use, and/or disposal of these products.









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