06/24/2008 Kit ingredients
Product: CaspACETM Assay System Colorimetric, 100rxns
Product code: G7220
Substance number Description Count Symbols
G595 Caspase Assay Buffer 1 -
G725 pNA Standard 1 Xn
G726 Cell Lysis Buffer, 20ml 1 -
G723 Caspase Inhibitor, Z-VAD-FMK 4 Xn
G724 Caspase-3 Substrate Ac-DEVD-pNA 8 Xn
Page 1/5
Safety Data Sheet
according to 1907/2006/EC, Article 31
Printing date 24.06.2008 Revision: 24.06.2008
* 1 Identification of substance
Trade name: Caspase Assay Buffer
Article number: G595
Application of the substance / the preparation: Laboratory chemicals
Manufacturer/Supplier:
Promega Corporation
2800 Woods Hollow Road
Madison, WI 53711
U.S.A.
1-608-274-4330
1-800-356-9526
MSDS author: Regulatory.Affairs@promega.com
Further information obtainable from:
Promega UK Ltd.
Delta House
Southampton Science Park
Southampton SO16 7NS
UNITED KINGDOM
Tel: (44) 23 8076 0225
Fax: (44) 23 8076 7014
FREEPHONE: 0800 378994
E-mail Address: ukcustserve@promega.com
Web Address: www.promega.com/uk/
Information in case of emergency:
CHEMTREC 1-800-424-9300
For call originating outside the United States dial 001-703-527-3887
2 Hazards identification
Hazard description: not applicable
Information concerning to particular hazards to man and environment
The product does not have to be labelled due to the calculation procedure of the "General Classification
guideline for preparations of the EU" in the latest valid version.
Classification system:
The classification is in line with current EC lists. It is extended, by information from technical literature and
company information.
* 3 Composition/information on ingredients
Chemical characterization
Description: Mixture of substances listed below with nonhazardous additions.
(Contd. on page 2)
GB
DR
Page 2/5
Safety Data Sheet
according to 1907/2006/EC, Article 31
Printing date 24.06.2008 Revision: 24.06.2008
Trade name: Caspase Assay Buffer
(Contd. of page 1)
Components:
CAS: 57-50-1 sucrose, pure 25-50%
EINECS: 200-334-9
Additional information: For the wording of the listed risk phrases refer to section 16.
4 First aid measures
General information: No special measures required.
After inhalation: Supply fresh air; consult doctor in case of complaints.
After skin contact: Generally the product does not irritate the skin.
After eye contact: Rinse opened eye for several minutes under running water.
After swallowing: If symptoms persist consult doctor.
5 Fire fighting measures
Suitable extinguishing agents:
CO2, powder or water spray. Fight larger fires with water spray or alcohol resistant foam.
Protective equipment: No special measures required.
6 Accidental release measures
Person-related safety precautions: Not required.
Measures for environmental protection: No special measures required.
Measures for cleaning/collecting:
Absorb with liquid-binding material (sand, diatomite, acid binders, universal binders, sawdust).
Additional information: No dangerous substances are released.
7 Handling and storage
Handling
Information for safe handling: No special measures required.
Information about fire - and explosion protection: No special measures required.
Storage
Requirements to be met by storerooms and receptacles: No special requirements.
Information about storage in one common storage facility: Not required.
Further information about storage conditions: None.
8 Exposure controls and personal protection gear
Additional information about design of technical facilities: No further data; see item 7.
Ingredients with limit values that require monitoring at the workplace:
57-50-1 sucrose, pure
WEL Short-term value: 20 mg/m?br>
Long-term value: 10 mg/m?br>
Additional information: The lists valid during the making were used as basis.
(Contd. on page 3)
GB
DR
Page 3/5
Safety Data Sheet
according to 1907/2006/EC, Article 31
Printing date 24.06.2008 Revision: 24.06.2008
Trade name: Caspase Assay Buffer
(Contd. of page 2)
Personal protective equipment
General protective and hygienic measures
The usual precautionary measures are to be adhered to when handling chemicals.
Respiratory protection: Not required.
Protection of hands:
Protective gloves.
Selection of the glove material on consideration of the penetration times, rates of diffusion and the degradation
Material of gloves:
The selection of the suitable gloves does not only depend on the material, but also on further marks of quality and
varies from manufacturer to manufacturer. As the product is a preparation of several substances, the resistance
of the glove material can not be calculated in advance and has therefore to be checked prior to the application.
Penetration time of glove material:
The exact break trough time has to be found out by the manufacturer of the protective gloves and has to be
observed.
Eye protection: Goggles recommended during refilling.
9 Physical and chemical properties
General Information
Fluid
Form:
Colourless
Colour:
Characteristic
Odour:
Change in condition
Melting point/Melting range: undetermined
Boiling point/Boiling range: 100癈
Not applicable
Flash point:
Product is not selfigniting.
Self-igniting:
Product does not present an explosion hazard.
Danger of explosion:
Not determined
Density:
Solubility in / Miscibility with
Not miscible or difficult to mix
Water:
Solvent content:
0.0 %
Organic solvents:
67.4 %
Water:
32.6 %
Solids content:
10 Stability and reactivity
Thermal decomposition / conditions to be avoided: No decomposition if used according to specifications.
Dangerous reactions: No dangerous reactions known
Dangerous decomposition products: No dangerous decomposition products known
GB
(Contd. on page 4)
DR
Page 4/5
Safety Data Sheet
according to 1907/2006/EC, Article 31
Printing date 24.06.2008 Revision: 24.06.2008
Trade name: Caspase Assay Buffer
(Contd. of page 3)
11 Toxicological information
Acute toxicity:
Primary irritant effect:
on the skin: No irritant effect.
on the eye: Irritating effect.
Sensitization: No sensitizing effects known.
Additional toxicological information:
The product is not subject to classification according to the calculation method of the General EU Classification
Guidelines for Preparations as issued in the latest version.
When used and handled according to specifications, the product does not have any harmful effects to our
experience and the information provided to us.
12 Ecological information
General notes: Generally not hazardous for water.
13 Disposal considerations
Product:
Recommendation
Smaller quantities can be disposed of with household waste.
Must not be disposed together with household garbage. Do not allow product to reach sewage system.
Uncleaned packaging:
Recommendation: Disposal must be made according to official regulations.
* 14 Transport information
Contact Promega Safety Department for additional transport information.
Land transport ADR/RID (cross-border)
ADR/RID class: -
Not regulated
Maritime transport IMDG:
-
IMDG Class:
Marine pollutant: No
Air transport ICAO-TI and IATA-DGR:
ICAO/IATA Class: -
15 Regulations
Labelling according to EU guidelines:
Observe the general safety regulations when handling chemicals
The product is not subject to identification regulations under EU Directives and the Ordinance on Hazardous
Materials (German GefStoffV).
(Contd. on page 5)
GB
DR
Page 5/5
Safety Data Sheet
according to 1907/2006/EC, Article 31
Printing date 24.06.2008 Revision: 24.06.2008
Trade name: Caspase Assay Buffer
(Contd. of page 4)
Special labelling of certain preparations:
Safety data sheet available for professional user on request.
National regulations
Waterhazard class: Generally not hazardous for water.
16 Other information
This information is based on our present knowledge. However, this shall not constitute a guarantee for any
specific product features and shall not establish a legally valid contractual relationship.
Department issuing MSDS:
Promega Corporation
Safety Department
2800 Woods Hollow Road
Madison, WI 53711
U.S.A.
Contact:
Promega UK
Delta House, Enterprise Road
Chilworth Science Park
Southampton SO16 7NS
UNITED KINGDOM
Free Phone +44 800 559900
Free Fax +44 800 181037
* Data compared to the previous version altered.
GB
DR
Page 1/6
Safety Data Sheet
according to 1907/2006/EC, Article 31
Printing date 24.06.2008 Revision: 24.06.2008
* 1 Identification of substance
Trade name: pNA Standard
Article number: G725
Application of the substance / the preparation: Laboratory chemicals
Manufacturer/Supplier:
Promega Corporation
2800 Woods Hollow Road
Madison, WI 53711
U.S.A.
1-608-274-4330
1-800-356-9526
MSDS author: Regulatory.Affairs@promega.com
Further information obtainable from:
Promega UK Ltd.
Delta House
Southampton Science Park
Southampton SO16 7NS
UNITED KINGDOM
Tel: (44) 23 8076 0225
Fax: (44) 23 8076 7014
FREEPHONE: 0800 378994
E-mail Address: ukcustserve@promega.com
Web Address: www.promega.com/uk/
Information in case of emergency:
CHEMTREC 1-800-424-9300
For call originating outside the United States dial 001-703-527-3887
2 Hazards identification
Hazard description:
Xn Harmful
Information concerning to particular hazards to man and environment
The product has to be labelled due to the calculation procedure of the "General Classification guideline for
preparations of the EU" in the latest valid version.
R 22 Harmful if swallowed.
R 36/38 Irritating to eyes and skin.
Classification system:
The classification is in line with current EC lists. It is extended, by information from technical literature and
company information.
GB
(Contd. on page 2)
DR
Page 2/6
Safety Data Sheet
according to 1907/2006/EC, Article 31
Printing date 24.06.2008 Revision: 24.06.2008
Trade name: pNA Standard
(Contd. of page 1)
3 Composition/information on ingredients
Chemical characterization
Description: Mixture of substances listed below with nonhazardous additions.
Components:
CAS: 67-68-5 dimethyl sulfoxide Xn, Xi; R 22-36/38 75-100%
EINECS: 200-664-3
CAS: 100-01-6 p-nitroaniline T; R 23/24/25-33-52/53 <1.00%
EINECS: 202-810-1
Additional information: For the wording of the listed risk phrases refer to section 16.
4 First aid measures
General information:
Symptoms of poisoning may even occur after several hours; therefore medical observation for at least 48 hours
after the accident.
After inhalation: In case of unconsciousness place patient stably in side position for transportation.
After skin contact: Immediately wash with soap, water and rinse thoroughly.
After eye contact:
Rinse opened eye for several minutes under running water. If symptoms persist, consult a doctor.
After swallowing: Call for a doctor immediately.
5 Fire fighting measures
Suitable extinguishing agents:
CO2, powder or water spray. Fight larger fires with water spray or alcohol resistant foam.
* 6 Accidental release measures
Person-related safety precautions: Not required.
Measures for environmental protection:
Dilute with plenty of water.
Do not allow to enter sewers/ surface or ground water.
Measures for cleaning/collecting:
Absorb with liquid-binding material (sand, diatomite, acid binders, universal binders, sawdust).
Dispose contaminated material as waste according to item 13.
Ensure adequate ventilation.
* 7 Handling and storage
Handling
Information for safe handling:
Keep receptacles tightly sealed.
Ensure good ventilation/exhaustion at the workplace.
Prevent formation of aerosols.
No special precautions are necessary if used correctly.
Information about fire - and explosion protection: No special measures required.
(Contd. on page 3)
GB
DR
Page 3/6
Safety Data Sheet
according to 1907/2006/EC, Article 31
Printing date 24.06.2008 Revision: 24.06.2008
Trade name: pNA Standard
(Contd. of page 2)
Storage
Requirements to be met by storerooms and receptacles: No special requirements.
Information about storage in one common storage facility: Not required.
Further information about storage conditions: Keep receptacle tightly sealed.
* 8 Exposure controls and personal protection gear
Additional information about design of technical facilities: No further data; see item 7.
Ingredients with limit values that require monitoring at the workplace:
The product does not contain any relevant quantities of materials with critical values that have to be monitored at
the workplace.
Additional information: The lists valid during the making were used as basis.
Personal protective equipment
General protective and hygienic measures
Keep away from foodstuffs, beverages and feed.
Immediately remove all soiled and contaminated clothing
Wash hands before breaks and at the end of work.
Avoid contact with the eyes and skin.
Respiratory protection:
In case of brief exposure or low pollution use respiratory filter device. In case of intensive or longer exposure use
self-contained respiratory protective device.
Protection of hands:
Protective gloves.
Selection of the glove material on consideration of the penetration times, rates of diffusion and the degradation
Material of gloves:
The selection of the suitable gloves does not only depend on the material, but also on further marks of quality and
varies from manufacturer to manufacturer. As the product is a preparation of several substances, the resistance
of the glove material can not be calculated in advance and has therefore to be checked prior to the application.
Penetration time of glove material:
The exact break trough time has to be found out by the manufacturer of the protective gloves and has to be
observed.
Eye protection: Tightly sealed goggles.
* 9 Physical and chemical properties
General Information
Fluid
Form:
Colourless
Colour:
Characteristic
Odour:
Change in condition
Melting point/Melting range: 18.45癈
Boiling point/Boiling range: 189癈
> 55癈
Flash point:
270癈
Ignition temperature:
Product is not selfigniting.
Self-igniting:
Product does not present an explosion hazard.
Danger of explosion:
(Contd. on page 4)
GB
DR
Page 4/6
Safety Data Sheet
according to 1907/2006/EC, Article 31
Printing date 24.06.2008 Revision: 24.06.2008
Trade name: pNA Standard
(Contd. of page 3)
Explosion limits:
1.8 Vol %
Lower:
Zers Vol %
Upper:
2.5 hPa
Vapour pressure at 20癈:
1.1 g/cm?br>
Density at 20癈:
Solubility in / Miscibility with
Fully miscible
Water:
Solvent content:
99.9 %
Organic solvents:
0.1 %
Solids content:
10 Stability and reactivity
Thermal decomposition / conditions to be avoided: No decomposition if used according to specifications.
Dangerous reactions:
Reacts with strong acids
Reacts with strong oxidizing agents
Dangerous decomposition products:
Sulphur oxides (SOx)
Carbon monoxide and carbon dioxide
11 Toxicological information
Acute toxicity:
LD/LC50 values relevant for classification:
67-68-5 dimethyl sulfoxide
Oral LD50 14500 mg/kg (Rat)
Irritation of eyes acute 500 mg (Rabbit)
Primary irritant effect:
on the skin: Irritant to skin and mucous membranes.
on the eye: Irritating effect.
Sensitization: No sensitizing effects known.
Additional toxicological information:
The product shows the following dangers according to the calculation method of the General EU Classification
Guidelines for Preparations as issued in the latest version.
Harmful
Irritant
12 Ecological information
General notes:
Water hazard class 1 (German Regulation) (Self-assessment): slightly hazardous for water.
Do not allow undiluted product or large quantities of it to reach ground water, water course or sewage system.
GB
(Contd. on page 5)
DR
Page 5/6
Safety Data Sheet
according to 1907/2006/EC, Article 31
Printing date 24.06.2008 Revision: 24.06.2008
Trade name: pNA Standard
(Contd. of page 4)
13 Disposal considerations
Product:
Recommendation
Must not be disposed together with household garbage. Do not allow product to reach sewage system.
Uncleaned packaging:
Recommendation: Disposal must be made according to official regulations.
Recommended cleansing agents: Water, if necessary together with cleansing agents.
* 14 Transport information
Contact Promega Safety Department for additional transport information.
Land transport ADR/RID (cross-border)
ADR/RID class: -
Not regulated
Maritime transport IMDG:
-
IMDG Class:
Marine pollutant: No
Air transport ICAO-TI and IATA-DGR:
ICAO/IATA Class: -
* 15 Regulations
Labelling according to EU guidelines:
The product has been classified and marked in accordance with EU Directives / Ordinance on Hazardous
Materials
Hazard-determining components of labelling:
dimethyl sulfoxide
Risk phrases:
22 Harmful if swallowed.
36/38 Irritating to eyes and skin.
Safety phrases:
23 Do not breathe gas/fumes/vapour/spray (appropriate wording to be specified by the manufacturer).
25 Avoid contact with eyes.
26 In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
36/37 Wear suitable protective clothing and gloves.
60 This material and its container must be disposed of as hazardous waste.
National regulations
Technical instructions (air):
Class Share in %
I 0.1
NK 99.9
(Contd. on page 6)
GB
DR
Page 6/6
Safety Data Sheet
according to 1907/2006/EC, Article 31
Printing date 24.06.2008 Revision: 24.06.2008
Trade name: pNA Standard
(Contd. of page 5)
Waterhazard class: Water hazard class 1 (Self-assessment): slightly hazardous for water.
16 Other information
This information is based on our present knowledge. However, this shall not constitute a guarantee for any
specific product features and shall not establish a legally valid contractual relationship.
Relevant R-phrases
22 Harmful if swallowed.
23/24/25 Toxic by inhalation, in contact with skin and if swallowed.
33 Danger of cumulative effects.
36/38 Irritating to eyes and skin.
52/53 Harmful to aquatic organisms, may cause long-term adverse effects in the aquatic environment.
Department issuing MSDS:
Promega Corporation
Safety Department
2800 Woods Hollow Road
Madison, WI 53711
U.S.A.
Contact:
Promega UK
Delta House, Enterprise Road
Chilworth Science Park
Southampton SO16 7NS
UNITED KINGDOM
Free Phone +44 800 559900
Free Fax +44 800 181037
* Data compared to the previous version altered.
GB
DR
Page 1/5
Safety Data Sheet
according to 1907/2006/EC, Article 31
Printing date 24.06.2008 Revision: 24.06.2008
* 1 Identification of substance
Trade name: Cell Lysis Buffer, 20ml
Article number: G726
Application of the substance / the preparation: Laboratory chemicals
Manufacturer/Supplier:
Promega Corporation
2800 Woods Hollow Road
Madison, WI 53711
U.S.A.
1-608-274-4330
1-800-356-9526
MSDS author: Regulatory.Affairs@promega.com
Further information obtainable from:
Promega UK Ltd.
Delta House
Southampton Science Park
Southampton SO16 7NS
UNITED KINGDOM
Tel: (44) 23 8076 0225
Fax: (44) 23 8076 7014
FREEPHONE: 0800 378994
E-mail Address: ukcustserve@promega.com
Web Address: www.promega.com/uk/
Information in case of emergency:
CHEMTREC 1-800-424-9300
For call originating outside the United States dial 001-703-527-3887
2 Hazards identification
Hazard description: not applicable
Information concerning to particular hazards to man and environment
The product does not have to be labelled due to the calculation procedure of the "General Classification
guideline for preparations of the EU" in the latest valid version.
Classification system:
The classification is in line with current EC lists. It is extended, by information from technical literature and
company information.
3 Composition/information on ingredients
Chemical characterization
Description: Mixture of substances listed below with nonhazardous additions.
Components: Void
(Contd. on page 2)
GB
DR
Page 2/5
Safety Data Sheet
according to 1907/2006/EC, Article 31
Printing date 24.06.2008 Revision: 24.06.2008
Trade name: Cell Lysis Buffer, 20ml
(Contd. of page 1)
Additional information: For the wording of the listed risk phrases refer to section 16.
4 First aid measures
General information: No special measures required.
After inhalation: Supply fresh air; consult doctor in case of complaints.
After skin contact: Generally the product does not irritate the skin.
After eye contact: Rinse opened eye for several minutes under running water.
After swallowing: If symptoms persist consult doctor.
5 Fire fighting measures
Suitable extinguishing agents:
CO2, powder or water spray. Fight larger fires with water spray or alcohol resistant foam.
Protective equipment: No special measures required.
6 Accidental release measures
Person-related safety precautions: Not required.
Measures for environmental protection: Dilute with plenty of water.
Measures for cleaning/collecting:
Absorb with liquid-binding material (sand, diatomite, acid binders, universal binders, sawdust).
Additional information: No dangerous substances are released.
7 Handling and storage
Handling
Information for safe handling: No special measures required.
Information about fire - and explosion protection: No special measures required.
Storage
Requirements to be met by storerooms and receptacles: No special requirements.
Information about storage in one common storage facility: Not required.
Further information about storage conditions: None.
8 Exposure controls and personal protection gear
Additional information about design of technical facilities: No further data; see item 7.
Ingredients with limit values that require monitoring at the workplace:
The product does not contain any relevant quantities of materials with critical values that have to be monitored at
the workplace.
Additional information: The lists valid during the making were used as basis.
Personal protective equipment
General protective and hygienic measures
The usual precautionary measures are to be adhered to when handling chemicals.
Respiratory protection: Not required.
Protection of hands:
Protective gloves.
Selection of the glove material on consideration of the penetration times, rates of diffusion and the degradation
(Contd. on page 3)
GB
DR
Page 3/5
Safety Data Sheet
according to 1907/2006/EC, Article 31
Printing date 24.06.2008 Revision: 24.06.2008
Trade name: Cell Lysis Buffer, 20ml
(Contd. of page 2)
Material of gloves:
The selection of the suitable gloves does not only depend on the material, but also on further marks of quality and
varies from manufacturer to manufacturer. As the product is a preparation of several substances, the resistance
of the glove material can not be calculated in advance and has therefore to be checked prior to the application.
Penetration time of glove material:
The exact break trough time has to be found out by the manufacturer of the protective gloves and has to be
observed.
Eye protection: Goggles recommended during refilling.
9 Physical and chemical properties
General Information
Fluid
Form:
Colourless
Colour:
Characteristic
Odour:
Change in condition
Melting point/Melting range: 0癈
Boiling point/Boiling range: 100癈
Not applicable
Flash point:
Product is not selfigniting.
Self-igniting:
Product does not present an explosion hazard.
Danger of explosion:
1 g/cm?br>
Density at 20癈:
Solubility in / Miscibility with
Fully miscible
Water:
Solvent content:
0.0 %
Organic solvents:
97.4 %
Water:
2.0 %
Solids content:
10 Stability and reactivity
Thermal decomposition / conditions to be avoided: No decomposition if used according to specifications.
Dangerous reactions: No dangerous reactions known
Dangerous decomposition products: No dangerous decomposition products known
11 Toxicological information
Acute toxicity:
Primary irritant effect:
on the skin: No irritant effect.
on the eye: Irritating effect.
Sensitization: No sensitizing effects known.
Additional toxicological information:
The product is not subject to classification according to the calculation method of the General EU Classification
Guidelines for Preparations as issued in the latest version.
(Contd. on page 4)
GB
DR
Page 4/5
Safety Data Sheet
according to 1907/2006/EC, Article 31
Printing date 24.06.2008 Revision: 24.06.2008
Trade name: Cell Lysis Buffer, 20ml
(Contd. of page 3)
When used and handled according to specifications, the product does not have any harmful effects to our
experience and the information provided to us.
12 Ecological information
General notes: Generally not hazardous for water.
13 Disposal considerations
Product:
Recommendation
Smaller quantities can be disposed of with household waste.
Must not be disposed together with household garbage. Do not allow product to reach sewage system.
Uncleaned packaging:
Recommendation: Disposal must be made according to official regulations.
Recommended cleansing agents: Water, if necessary together with cleansing agents.
* 14 Transport information
Contact Promega Safety Department for additional transport information.
Land transport ADR/RID (cross-border)
ADR/RID class: -
Not regulated
Maritime transport IMDG:
-
IMDG Class:
Marine pollutant: No
Air transport ICAO-TI and IATA-DGR:
ICAO/IATA Class: -
15 Regulations
Labelling according to EU guidelines:
Observe the general safety regulations when handling chemicals
The product is not subject to identification regulations under EU Directives and the Ordinance on Hazardous
Materials (German GefStoffV).
National regulations
Waterhazard class: Generally not hazardous for water.
16 Other information
This information is based on our present knowledge. However, this shall not constitute a guarantee for any
specific product features and shall not establish a legally valid contractual relationship.
(Contd. on page 5)
GB
DR
Page 5/5
Safety Data Sheet
according to 1907/2006/EC, Article 31
Printing date 24.06.2008 Revision: 24.06.2008
Trade name: Cell Lysis Buffer, 20ml
(Contd. of page 4)
Department issuing MSDS:
Promega Corporation
Safety Department
2800 Woods Hollow Road
Madison, WI 53711
U.S.A.
Contact:
Promega UK
Delta House, Enterprise Road
Chilworth Science Park
Southampton SO16 7NS
UNITED KINGDOM
Free Phone +44 800 559900
Free Fax +44 800 181037
* Data compared to the previous version altered.
GB
DR
Page 1/6
Safety Data Sheet
according to 1907/2006/EC, Article 31
Printing date 24.06.2008 Revision: 24.06.2008
* 1 Identification of substance
Trade name: Caspase Inhibitor, Z-VAD-FMK
Article number: G723
Application of the substance / the preparation: Laboratory chemicals
Manufacturer/Supplier:
Promega Corporation
2800 Woods Hollow Road
Madison, WI 53711
U.S.A.
1-608-274-4330
1-800-356-9526
MSDS author: Regulatory.Affairs@promega.com
Further information obtainable from:
Promega UK Ltd.
Delta House
Southampton Science Park
Southampton SO16 7NS
UNITED KINGDOM
Tel: (44) 23 8076 0225
Fax: (44) 23 8076 7014
FREEPHONE: 0800 378994
E-mail Address: ukcustserve@promega.com
Web Address: www.promega.com/uk/
Information in case of emergency:
CHEMTREC 1-800-424-9300
For call originating outside the United States dial 001-703-527-3887
2 Hazards identification
Hazard description:
Xn Harmful
Information concerning to particular hazards to man and environment
The product has to be labelled due to the calculation procedure of the "General Classification guideline for
preparations of the EU" in the latest valid version.
R 22 Harmful if swallowed.
R 36/38 Irritating to eyes and skin.
Classification system:
The classification is in line with current EC lists. It is extended, by information from technical literature and
company information.
GB
(Contd. on page 2)
DR
Page 2/6
Safety Data Sheet
according to 1907/2006/EC, Article 31
Printing date 24.06.2008 Revision: 24.06.2008
Trade name: Caspase Inhibitor, Z-VAD-FMK
(Contd. of page 1)
3 Composition/information on ingredients
Chemical characterization
Description: Mixture of substances listed below with nonhazardous additions.
Components:
CAS: 67-68-5 dimethyl sulfoxide Xn, Xi; R 22-36/38 75-100%
EINECS: 200-664-3
Additional information: For the wording of the listed risk phrases refer to section 16.
4 First aid measures
General information:
Symptoms of poisoning may even occur after several hours; therefore medical observation for at least 48 hours
after the accident.
After inhalation: In case of unconsciousness place patient stably in side position for transportation.
After skin contact: Immediately wash with soap, water and rinse thoroughly.
After eye contact:
Rinse opened eye for several minutes under running water. If symptoms persist, consult a doctor.
After swallowing: Call for a doctor immediately.
5 Fire fighting measures
Suitable extinguishing agents:
CO2, powder or water spray. Fight larger fires with water spray or alcohol resistant foam.
* 6 Accidental release measures
Person-related safety precautions: Not required.
Measures for environmental protection:
Dilute with plenty of water.
Do not allow to enter sewers/ surface or ground water.
Measures for cleaning/collecting:
Absorb with liquid-binding material (sand, diatomite, acid binders, universal binders, sawdust).
Dispose contaminated material as waste according to item 13.
Ensure adequate ventilation.
* 7 Handling and storage
Handling
Information for safe handling:
Keep receptacles tightly sealed.
Ensure good ventilation/exhaustion at the workplace.
Prevent formation of aerosols.
No special precautions are necessary if used correctly.
Information about fire - and explosion protection: No special measures required.
Storage
Requirements to be met by storerooms and receptacles: No special requirements.
Information about storage in one common storage facility: Not required.
(Contd. on page 3)
GB
DR
Page 3/6
Safety Data Sheet
according to 1907/2006/EC, Article 31
Printing date 24.06.2008 Revision: 24.06.2008
Trade name: Caspase Inhibitor, Z-VAD-FMK
(Contd. of page 2)
Further information about storage conditions: Keep receptacle tightly sealed.
* 8 Exposure controls and personal protection gear
Additional information about design of technical facilities: No further data; see item 7.
Ingredients with limit values that require monitoring at the workplace:
The product does not contain any relevant quantities of materials with critical values that have to be monitored at
the workplace.
Additional information: The lists valid during the making were used as basis.
Personal protective equipment
General protective and hygienic measures
Keep away from foodstuffs, beverages and feed.
Immediately remove all soiled and contaminated clothing
Wash hands before breaks and at the end of work.
Avoid contact with the eyes and skin.
Respiratory protection:
In case of brief exposure or low pollution use respiratory filter device. In case of intensive or longer exposure use
self-contained respiratory protective device.
Protection of hands:
Protective gloves.
Selection of the glove material on consideration of the penetration times, rates of diffusion and the degradation
Material of gloves:
The selection of the suitable gloves does not only depend on the material, but also on further marks of quality and
varies from manufacturer to manufacturer. As the product is a preparation of several substances, the resistance
of the glove material can not be calculated in advance and has therefore to be checked prior to the application.
Penetration time of glove material:
The exact break trough time has to be found out by the manufacturer of the protective gloves and has to be
observed.
Eye protection: Tightly sealed goggles.
9 Physical and chemical properties
General Information
Fluid
Form:
Colourless
Colour:
Characteristic
Odour:
Change in condition
Melting point/Melting range: 18.45癈
Boiling point/Boiling range: 189癈
> 55癈
Flash point:
270癈
Ignition temperature:
Product is not selfigniting.
Self-igniting:
Product does not present an explosion hazard.
Danger of explosion:
Explosion limits:
1.8 Vol %
Lower:
Zers Vol %
Upper:
(Contd. on page 4)
GB
DR
Page 4/6
Safety Data Sheet
according to 1907/2006/EC, Article 31
Printing date 24.06.2008 Revision: 24.06.2008
Trade name: Caspase Inhibitor, Z-VAD-FMK
(Contd. of page 3)
2.5 hPa
Vapour pressure at 20癈:
1.1 g/cm?br>
Density at 20癈:
Solubility in / Miscibility with
Fully miscible
Water:
Solvent content:
99.0 %
Organic solvents:
1.0 %
Solids content:
10 Stability and reactivity
Thermal decomposition / conditions to be avoided: No decomposition if used according to specifications.
Dangerous reactions:
Reacts with strong oxidizing agents
Reacts with strong acids
Dangerous decomposition products:
Sulphur oxides (SOx)
Carbon monoxide and carbon dioxide
11 Toxicological information
Acute toxicity:
LD/LC50 values relevant for classification:
67-68-5 dimethyl sulfoxide
Oral LD50 14500 mg/kg (Rat)
Irritation of eyes acute 500 mg (Rabbit)
Primary irritant effect:
on the skin: Irritant to skin and mucous membranes.
on the eye: Irritating effect.
Sensitization: No sensitizing effects known.
Additional toxicological information:
The product shows the following dangers according to the calculation method of the General EU Classification
Guidelines for Preparations as issued in the latest version.
Harmful
Irritant
12 Ecological information
General notes:
Water hazard class 1 (German Regulation) (Self-assessment): slightly hazardous for water.
Do not allow undiluted product or large quantities of it to reach ground water, water course or sewage system.
GB
(Contd. on page 5)
DR
Page 5/6
Safety Data Sheet
according to 1907/2006/EC, Article 31
Printing date 24.06.2008 Revision: 24.06.2008
Trade name: Caspase Inhibitor, Z-VAD-FMK
(Contd. of page 4)
13 Disposal considerations
Product:
Recommendation
Must not be disposed together with household garbage. Do not allow product to reach sewage system.
Uncleaned packaging:
Recommendation: Disposal must be made according to official regulations.
Recommended cleansing agents: Water, if necessary together with cleansing agents.
* 14 Transport information
Contact Promega Safety Department for additional transport information.
Land transport ADR/RID (cross-border)
ADR/RID class: -
Not regulated
Maritime transport IMDG:
-
IMDG Class:
Marine pollutant: No
Air transport ICAO-TI and IATA-DGR:
ICAO/IATA Class: -
* 15 Regulations
Labelling according to EU guidelines:
The product has been classified and marked in accordance with EU Directives / Ordinance on Hazardous
Materials
Hazard-determining components of labelling:
dimethyl sulfoxide
Risk phrases:
22 Harmful if swallowed.
36/38 Irritating to eyes and skin.
Safety phrases:
23 Do not breathe gas/fumes/vapour/spray (appropriate wording to be specified by the manufacturer).
25 Avoid contact with eyes.
26 In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
36/37 Wear suitable protective clothing and gloves.
60 This material and its container must be disposed of as hazardous waste.
National regulations
Technical instructions (air):
Class Share in %
NK 99.0
Waterhazard class: Water hazard class 1 (Self-assessment): slightly hazardous for water.
GB
(Contd. on page 6)
DR
Page 6/6
Safety Data Sheet
according to 1907/2006/EC, Article 31
Printing date 24.06.2008 Revision: 24.06.2008
Trade name: Caspase Inhibitor, Z-VAD-FMK
(Contd. of page 5)
16 Other information
This information is based on our present knowledge. However, this shall not constitute a guarantee for any
specific product features and shall not establish a legally valid contractual relationship.
Relevant R-phrases
22 Harmful if swallowed.
36/38 Irritating to eyes and skin.
Department issuing MSDS:
Promega Corporation
Safety Department
2800 Woods Hollow Road
Madison, WI 53711
U.S.A.
Contact:
Promega UK
Delta House, Enterprise Road
Chilworth Science Park
Southampton SO16 7NS
UNITED KINGDOM
Free Phone +44 800 559900
Free Fax +44 800 181037
* Data compared to the previous version altered.
GB
DR
Page 1/6
Safety Data Sheet
according to 1907/2006/EC, Article 31
Printing date 24.06.2008 Revision: 24.06.2008
* 1 Identification of substance
Trade name: Caspase-3 Substrate Ac-DEVD-pNA
Article number: G724
Application of the substance / the preparation: Laboratory chemicals
Manufacturer/Supplier:
Promega Corporation
2800 Woods Hollow Road
Madison, WI 53711
U.S.A.
1-608-274-4330
1-800-356-9526
MSDS author: Regulatory.Affairs@promega.com
Further information obtainable from:
Promega UK Ltd.
Delta House
Southampton Science Park
Southampton SO16 7NS
UNITED KINGDOM
Tel: (44) 23 8076 0225
Fax: (44) 23 8076 7014
FREEPHONE: 0800 378994
E-mail Address: ukcustserve@promega.com
Web Address: www.promega.com/uk/
Information in case of emergency:
CHEMTREC 1-800-424-9300
For call originating outside the United States dial 001-703-527-3887
2 Hazards identification
Hazard description:
Xn Harmful
Information concerning to particular hazards to man and environment
The product has to be labelled due to the calculation procedure of the "General Classification guideline for
preparations of the EU" in the latest valid version.
R 22 Harmful if swallowed.
R 36/38 Irritating to eyes and skin.
Classification system:
The classification is in line with current EC lists. It is extended, by information from technical literature and
company information.
GB
(Contd. on page 2)
DR
Page 2/6
Safety Data Sheet
according to 1907/2006/EC, Article 31
Printing date 24.06.2008 Revision: 24.06.2008
Trade name: Caspase-3 Substrate Ac-DEVD-pNA
(Contd. of page 1)
3 Composition/information on ingredients
Chemical characterization
Description: Mixture of substances listed below with nonhazardous additions.
Components:
CAS: 67-68-5 dimethyl sulfoxide Xn, Xi; R 22-36/38 75-100%
EINECS: 200-664-3
Additional information: For the wording of the listed risk phrases refer to section 16.
4 First aid measures
General information:
Symptoms of poisoning may even occur after several hours; therefore medical observation for at least 48 hours
after the accident.
After inhalation: In case of unconsciousness place patient stably in side position for transportation.
After skin contact: Immediately wash with soap, water and rinse thoroughly.
After eye contact:
Rinse opened eye for several minutes under running water. If symptoms persist, consult a doctor.
After swallowing: Call for a doctor immediately.
5 Fire fighting measures
Suitable extinguishing agents:
CO2, powder or water spray. Fight larger fires with water spray or alcohol resistant foam.
* 6 Accidental release measures
Person-related safety precautions: Not required.
Measures for environmental protection:
Dilute with plenty of water.
Do not allow to enter sewers/ surface or ground water.
Measures for cleaning/collecting:
Absorb with liquid-binding material (sand, diatomite, acid binders, universal binders, sawdust).
Dispose contaminated material as waste according to item 13.
Ensure adequate ventilation.
* 7 Handling and storage
Handling
Information for safe handling:
Keep receptacles tightly sealed.
Ensure good ventilation/exhaustion at the workplace.
Prevent formation of aerosols.
No special precautions are necessary if used correctly.
Information about fire - and explosion protection: No special measures required.
Storage
Requirements to be met by storerooms and receptacles: No special requirements.
Information about storage in one common storage facility: Not required.
(Contd. on page 3)
GB
DR
Page 3/6
Safety Data Sheet
according to 1907/2006/EC, Article 31
Printing date 24.06.2008 Revision: 24.06.2008
Trade name: Caspase-3 Substrate Ac-DEVD-pNA
(Contd. of page 2)
Further information about storage conditions: Keep receptacle tightly sealed.
* 8 Exposure controls and personal protection gear
Additional information about design of technical facilities: No further data; see item 7.
Ingredients with limit values that require monitoring at the workplace:
The product does not contain any relevant quantities of materials with critical values that have to be monitored at
the workplace.
Additional information: The lists valid during the making were used as basis.
Personal protective equipment
General protective and hygienic measures
Keep away from foodstuffs, beverages and feed.
Immediately remove all soiled and contaminated clothing
Wash hands before breaks and at the end of work.
Avoid contact with the eyes and skin.
Respiratory protection:
In case of brief exposure or low pollution use respiratory filter device. In case of intensive or longer exposure use
self-contained respiratory protective device.
Protection of hands:
Protective gloves.
Selection of the glove material on consideration of the penetration times, rates of diffusion and the degradation
Material of gloves:
The selection of the suitable gloves does not only depend on the material, but also on further marks of quality and
varies from manufacturer to manufacturer. As the product is a preparation of several substances, the resistance
of the glove material can not be calculated in advance and has therefore to be checked prior to the application.
Penetration time of glove material:
The exact break trough time has to be found out by the manufacturer of the protective gloves and has to be
observed.
Eye protection: Tightly sealed goggles.
9 Physical and chemical properties
General Information
Fluid
Form:
Colourless
Colour:
Characteristic
Odour:
Change in condition
Melting point/Melting range: 18.45癈
Boiling point/Boiling range: 189癈
> 55癈
Flash point:
270癈
Ignition temperature:
Product is not selfigniting.
Self-igniting:
Product does not present an explosion hazard.
Danger of explosion:
Explosion limits:
1.8 Vol %
Lower:
Zers Vol %
Upper:
(Contd. on page 4)
GB
DR
Page 4/6
Safety Data Sheet
according to 1907/2006/EC, Article 31
Printing date 24.06.2008 Revision: 24.06.2008
Trade name: Caspase-3 Substrate Ac-DEVD-pNA
(Contd. of page 3)
2.5 hPa
Vapour pressure at 20癈:
1.1 g/cm?br>
Density at 20癈:
Solubility in / Miscibility with
Fully miscible
Water:
Solvent content:
99.3 %
Organic solvents:
0.7 %
Solids content:
10 Stability and reactivity
Thermal decomposition / conditions to be avoided: No decomposition if used according to specifications.
Dangerous reactions:
Reacts with strong oxidizing agents
Reacts with strong acids
Dangerous decomposition products:
Sulphur oxides (SOx)
Carbon monoxide and carbon dioxide
11 Toxicological information
Acute toxicity:
LD/LC50 values relevant for classification:
67-68-5 dimethyl sulfoxide
Oral LD50 14500 mg/kg (Rat)
Irritation of eyes acute 500 mg (Rabbit)
Primary irritant effect:
on the skin: Irritant to skin and mucous membranes.
on the eye: Irritating effect.
Sensitization: No sensitizing effects known.
Additional toxicological information:
The product shows the following dangers according to the calculation method of the General EU Classification
Guidelines for Preparations as issued in the latest version.
Harmful
Irritant
12 Ecological information
General notes:
Water hazard class 1 (German Regulation) (Self-assessment): slightly hazardous for water.
Do not allow undiluted product or large quantities of it to reach ground water, water course or sewage system.
GB
(Contd. on page 5)
DR
Page 5/6
Safety Data Sheet
according to 1907/2006/EC, Article 31
Printing date 24.06.2008 Revision: 24.06.2008
Trade name: Caspase-3 Substrate Ac-DEVD-pNA
(Contd. of page 4)
13 Disposal considerations
Product:
Recommendation
Must not be disposed together with household garbage. Do not allow product to reach sewage system.
Uncleaned packaging:
Recommendation: Disposal must be made according to official regulations.
Recommended cleansing agents: Water, if necessary together with cleansing agents.
* 14 Transport information
Contact Promega Safety Department for additional transport information.
Land transport ADR/RID (cross-border)
ADR/RID class: -
Not regulated
Maritime transport IMDG:
-
IMDG Class:
Marine pollutant: No
Air transport ICAO-TI and IATA-DGR:
ICAO/IATA Class: -
* 15 Regulations
Labelling according to EU guidelines:
The product has been classified and marked in accordance with EU Directives / Ordinance on Hazardous
Materials
Hazard-determining components of labelling:
dimethyl sulfoxide
Risk phrases:
22 Harmful if swallowed.
36/38 Irritating to eyes and skin.
Safety phrases:
23 Do not breathe gas/fumes/vapour/spray (appropriate wording to be specified by the manufacturer).
25 Avoid contact with eyes.
26 In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
36/37 Wear suitable protective clothing and gloves.
60 This material and its container must be disposed of as hazardous waste.
National regulations
Technical instructions (air):
Class Share in %
NK 99.3
Waterhazard class: Water hazard class 1 (Self-assessment): slightly hazardous for water.
GB
(Contd. on page 6)
DR
Page 6/6
Safety Data Sheet
according to 1907/2006/EC, Article 31
Printing date 24.06.2008 Revision: 24.06.2008
Trade name: Caspase-3 Substrate Ac-DEVD-pNA
(Contd. of page 5)
16 Other information
This information is based on our present knowledge. However, this shall not constitute a guarantee for any
specific product features and shall not establish a legally valid contractual relationship.
Relevant R-phrases
22 Harmful if swallowed.
36/38 Irritating to eyes and skin.
Department issuing MSDS:
Promega Corporation
Safety Department
2800 Woods Hollow Road
Madison, WI 53711
U.S.A.
Contact:
Promega UK
Delta House, Enterprise Road
Chilworth Science Park
Southampton SO16 7NS
UNITED KINGDOM
Free Phone +44 800 559900
Free Fax +44 800 181037
* Data compared to the previous version altered.
GB
DR
|