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MSDS Material Safety Data Sheet
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File Name: addgene_org_msds03272007.asp

                                                   One Kendall Square, Building 600 3rd Flr, Cambridge, MA 02139
info@addgene.org 鈥? Tel: 617-225-9000 鈥? Fax: 888-734-0533



MATERIAL SAFETY DATA SHEET Last Updated: 03/10/2006
E. coli bacteria with plasmids Version 1.02

SECTION 1: PRODUCT AND COMPANY IDENTIFICATION

PRODUCT NAME: E. coli bacteria with plasmids

COMPANY: Addgene
rd
STREET ADDRESS: One Kendall Square, Building 600, 3 Floor
CITY, STATE, ZIP, COUNTRY: Cambridge, MA 02139 US

TELEPHONE: (617) 225-9000
FAX: (888) 734-0533

PRODUCT USE: For laboratory research use only.


SECTION 2: COMPOSITION/INFORMATION ON INGREDIENTS


SUBSTANCE: E. coli bacteria

CAS NO. None

SARA 313 REPORTABLE No


SECTION 3: HAZARDS IDENTIFICATION

ROUTES OF ENTRY: Skin or eye contact

POTENTIAL HEALTH EFFECTS

EYES: May cause irritation of the eye

SKIN: May cause irritation of the skin.

INGESTION: May cause nausea or vomiting.

INHALATION: No toxicity expected from inhalation.


CARCINOGENICITY Not listed by OSHA, IARC, or NTP


SECTION 4: FIRST AID MEASURES

EYES: Rinse opened eyes for at least 15 minutes. If eyes become irritated, get medical
attention.

SKIN: Wash skin with soap and water.

INGESTION: Rinse mouth. If irritation or symptoms occur, get medical attention.

INHALATION: Find fresh air. If symptoms occur, get medical attention.


SECTION 5: FIRE-FIGHTING MEASURES

EXTINGUISHING MEDIA: Water, carbon dioxide, dry chemical powder, or foam

SPECIAL PROCEDURES: Wear self-contained breathing apparatus and protective clothing to prevent
contact with skin and eyes.


Page 1 of 3
SECTION 6: ACCIDENTAL RELEASE MEASURES

ENVIRONMENTAL PRECAUTIONS: Keep out of sewers and waterways.

CLEAN-UP METHODS: Clean spills with chlorine bleach or 70% ethanol. Autoclave materials and
dispose of waste in accordance with federal, state, and local safety regulations.


SECTION 7: HANDLING AND STORAGE

HANDLING: Avoid inhalation and contact with eyes, skin, and clothing.

STORAGE: Store at 4掳C. Create frozen stocks for long-term storage.


SECTION 8: EXPOSURE CONTROLS/PERSONAL PROTECTION

ENGINEERING CONTROLS: Safety shower and eye bath

VENTILATION : General ventilation

RESPIRATORY PROTECTION: Not required

EYE PROTECTION: Safety goggles

SKIN PROTECTION: Protective gloves

WORK HYGIENIC PRACTICES: Use safe laboratory practices. Handle as biohazard material.


SECTION 9: PHYSICAL AND CHEMICAL PROPERTIES

FORM: Bacteria supplied in glass vial with LB agar.

ODOR: Odorless

COLOR: Colorless


SECTION 10: STABILITY AND REACTIVITY

STABILITY: Stable

CONDITIONS TO AVOID: None known

MATERIALS TO AVOID: None known

HAZARDOUS DECOMPOSITION
OR BY-PRODUCTS: None known

HAZARDOUS POLYMERIZATION: None


SECTION 11: TOXICOLOGICAL INFORMATION

TOXICOLOGICAL INFORMATION: May be irritating to eyes and skin.


SECTION 12: ECOLOGICAL INFORMATION

ECOLOGICAL INFORMATION: No data.


SECTION 13: DISPOSAL CONSIDERATIONS



Page 2 of 3
WASTE DISPOSAL METHOD: Observe all federal, state, and local laws and regulations.

RCRA HAZARD CLASS: Not applicable.


SECTION 14: TRANSPORT INFORMATION

U.S. DEPARTMENT OF TRANSPORTATION
HAZARD CLASS: Non-hazardous

WATER TRANSPORTATION
HAZARD CLASS: Non-hazardous

AIR TRANSPORTATION
HAZARD CLASS: Non-hazardous


SECTION 15: REGULATORY INFORMATION

OSHA REGULATORY STATUS Irritant
(29 CFR 1910.1200)

TSCA: No specific regulations

CERCLA: None

SARA TITLE III: None

SARA 311/312 HAZARD CATEGORIES: None

SARA 313 REPORTABLE INGREDIENTS: None

STATE REGULATIONS: N/A


SECTION 16: OTHER INFORMATION

DISCLAIMER: For R&D use only. Not for drug, household or other uses.

The above information is correct to the best of our knowledge, but does not purport to be all inclusive and shall be used
only as a guide. Addgene shall not be held liable for any damage resulting from handling or from contact with the above
product.




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