Material Safety Data Sheet
Authorization date: 08/07/2007
Revision date: 09/14/2007
Print Date: 09/14/2007
Version: 1
1. IDENTIFICATION OF THE SUBSTANCE/PREPARATION AND THE COMPANY/UNDERTAKING
Product name: DIG Oligonucleotide Tailing Labeling Kit 2nd generation
Product number: 03353583910
Business area: Roche Applied Sciences
Product line: Not applicable
Instrument type: Not applicable
Supplier: Emergency telephone number:
Roche Diagnostics Corporation CHEMTREC:
9115 Hague Road 1-800-424-9300 (U.S. or Canada)
Indianapolis, IN 46250 1-703-527-3887 (International)
Site Phone Number: 1-800-428-5074
2. COMPOSITION/INFORMATION ON INGREDIENTS
Description 1: Reaction buffer 5x
Components CAS Number Weight % OSHA PEL: OSHA STEL: ACGIH TLV: ACGIH STEL:
Potassium cacodylate 21416-85-3 10 - 20 NA NA NA NA
Tris hydrochloride 1185-53-1 1-5 NA NA NA NA
Description 2: CoCl2 solution
Components CAS Number Weight % OSHA PEL: OSHA STEL: ACGIH TLV: ACGIH STEL:
0.1 mg/m3 dust 0.02 mg/m3
Cobalt (II) chloride, hexahydrate 7791-13-1 0.1-<1 NA NA
0.02 mg/m3 as Co
and fume
Description 3: DIG-ddUTP solution
Components CAS Number Weight % OSHA PEL: OSHA STEL: ACGIH TLV: ACGIH STEL:
Digoxigenin-11-ddUTP 127264-48-6 0.1-<1 NA NA NA NA
Description 4: 25 碌l terminal transferase, recombinant
Components CAS Number Weight % OSHA PEL: OSHA STEL: ACGIH TLV: ACGIH STEL:
15 mg/m3 total 10 mg/m3
Glycerol 56-81-5 50 - 60 NA NA
dust
5 mg/m3
respirable fraction
Potassium chloride 7447-40-7 1-5 NA NA NA NA
Description 5: All other bottles
Components CAS Number Weight % OSHA PEL: OSHA STEL: ACGIH TLV: ACGIH STEL:
No hazardous components >1%, NONE <0.01 NA NA NA NA
>0.1% carcinogen
3. HAZARDS IDENTIFICATION
Emergency Overview
NFPA Ratings:
Reaction buffer 5x & DIG-ddUTP solution: Health= 3 Flammability= 0 Reactivity= 0 Special= T
CoCl2 solution: Health= 3 Flammability= 0 Reactivity= 0 Special= CA
All other solutions noted in section 2: Health= 1 Flammability= 0 Reactivity= 0
Special Definitions: A=Allergen CA=Carcinogen CO=Corrosive F=Flammable H=Harmful I=Irritant Ox=Oxidizer PB=Potential Biohazard
R=Reproductive S=Sensitizer T=Toxic T+=Highly Toxic W=Water Reactive
Principle routes of exposure: Ingestion, inhalation, skin absorption, skin and/or eye contact.
Inhalation: Avoid breathing vapors or mists. Arsenic compounds (i.e. cacodylate compounds) are toxic by inhalation.
All solutions with NFPA rating of 1 or higher... May cause irritation of respiratory tract.
Ingestion: Arsenic compounds (i.e. cacodylate compounds) are toxic if swallowed. Components of the product may
be absorbed into the body by inhalation, ingestion and through the skin. All solutions with NFPA rating of 1
or higher... Ingestion may cause gastrointestinal irritation, nausea, vomiting and diarrhea.
Skin contact: Arsenic compounds (e.g. cacodylate compounds) - May be absorbed through the skin in harmful amounts.
Eye contact: May cause irritation.
Sensitization or Odor threshold: The following chemical(s) may cause sensitization, be absorbed via skin, and/or has an odor threshold for
detection:
Components ACGIH - Germany (DFG) - Switzerland - AIHA - Odor Threshold
Sensitizer Designation: Skin/Sensitizers: Sensitizers: Values:
Cobalt (II) chloride, hexahydrate respiratory and skin Sensitizer
sensitizer
Medical conditions aggravated by Persons with pre-existing skin disorders or impaired liver, kidney, and pulmonary function may be more
exposure: susceptible to the effects of this material.
Additional information: GLYCEROL - May cause kidney injury with chronic exposure.
4. FIRST AID MEASURES
Inhalation: Consult a physician. Move to fresh air. If not breathing, give artificial respiration. If breathing is difficult, give
oxygen.
Skin contact: Rinse thoroughly with plenty of water for at least 15 minutes and consult a physician.
Ingestion: Call a physician immediately. Do not induce vomiting without medical advice. Drink 1 or 2 glasses of water.
Eye contact: Rinse thoroughly with plenty of water for at least 15 minutes and consult a physician.
Notes to physician: None determined
5. FIRE-FIGHTING MEASURES
Suitable extinguishing media: Use dry chemical, CO2, water spray or "alcohol" foam.
Unusual hazards: None known.
Special protective equipment for In the event of fire, wear self-contained breathing apparatus.
firefighters:
6. ACCIDENTAL RELEASE MEASURES
Personal precautions: Use personal protective equipment. Evacuate personnel to safe areas. Remove all sources of ignition.
Avoid dust formation. Ensure adequate ventilation.
Environmental precautions: Prevent further leakage or spillage if safe to do so. Prevent product from entering drains. Do not flush into
surface water or sanitary sewer system. Do not let product enter drains.
Methods for cleaning up: Never return spills in original containers for re-use. Soak up with oil absorbent material. Sweep up or
vacuum (if powder) or soak up with inert absorbent material (if liquid), then place into a suitable clean, dry,
closed container, and label for disposal.
7. HANDLING AND STORAGE
Handling: Protect from contamination. Wear personal protective equipment.
Storage: Keep containers dry and tightly closed to avoid moisture absorption and contamination.
8. EXPOSURE CONTROLS / PERSONAL PROTECTION
Engineering measures: Ensure adequate ventilation.
Personal Protective Equipment
Respiratory protection: Respiratory protection is not required under normal use of this product. If respiratory protection is needed,
follow the OSHA regulation, 29CFR1910.134. Always use a NIOSH approved respirator when necessary.
Hand protection: Wear appropriate protective gloves to prevent skin contact. Replace torn or punctured gloves promptly.
Product number: 03353583910 Page 2 of 5
Product name: DIG Oligonucleotide Tailing Labeling Kit 2nd generation
Skin and body protection: Wear appropriate body protection to prevent skin contact.
Eye protection: Wear appropriate eye protection to prevent eye contact.
Hygiene measures: Wash hands before breaks and immediately after handling the product. Handle in accordance with good
industrial hygiene and safety practice for diagnostics. When using, do not eat, drink or smoke. Keep away
from food and drink. Keep away from tobacco products.
9. PHYSICAL AND CHEMICAL PROPERTIES
Boiling point/range: No information available
Melting point/range: Not determined
pH: 5-9
Physical state: Liquid.
Solubility in water: Soluble.
Flash point: No information available.
LEL (%): Not determined
UEL (%): Not determined
10. STABILITY AND REACTIVITY
Stability: Stable under recommended storage conditions.
Polymerization: None under normal processing.
Hazardous decomposition products: Carbon oxides. Cobalt oxides. Hydrogen chloride.
Materials to avoid: Incompatible with strong acids and bases. Oxidising agents (strong). Heavy metals.
11. TOXICOLOGICAL INFORMATION
Components NIOSH - NIOSH Pocket Guide -
Selected LD50s and LC50s: Target Organs:
Cobalt (II) chloride, hexahydrate =6170 mg/kg Oral LD50 Rat respiratory system, skin
=766 mg/kg Oral LD50 Rat
>10 mg/L Inhalation LC50 Rat 1h
Components NIOSH - NIOSH Pocket Guide -
Selected LD50s and LC50s: Target Organs:
Glycerol =12600 mg/kg Oral LD50 Rat respiratory system, skin, eyes, kidneys
>10 g/kg Dermal LD50 Rabbit
>21900 mg/kg Dermal LD50 Rat
>570 mg/m3 Inhalation LC50 Rat 1h
Potassium chloride =2600 mg/kg Oral LD50 Rat
Inhalation: No additional data available
Skin: No additional data available
LD50/oral/rat =2600mg/m3 for sodium cacodylate. Digoxigenin: LD50/dog/oral = 0.422 mg/kg.
Oral:
Additional information: Sodium or potassium cacodylate may cause nephrotoxicity, hepatotoxicity, and may produce abnormalities
of the hematopoietic system.
Mutagenic effects: No data is available on the product itself
Reproductive toxicity: No data is available on the product itself
Carcinogenic effects:
Components IARC IARC IARC NTP OSHA EPA IRIS
Group 1 Group 2A or 2B Group 3 or 4 Select Carcinogen
Carcinogens Assessment
Cobalt (II) chloride, hexahydrate Monograph 52, Present
1991 (listed under
Cobalt and Cobalt
compounds)
12. ECOLOGICAL INFORMATION
Bioaccumulation: Not determined
Aquatic toxicity: No data is available on the product itself
Ecotoxicity effects: No data is available on the product itself
Product number: 03353583910 Page 3 of 5
Product name: DIG Oligonucleotide Tailing Labeling Kit 2nd generation
Components Ecotoxicity - Ecotoxicity - Ecotoxicity - Ecotoxicity -
Fish Species Data Microtox Data Water Flea Data Freshwater Algae Data
Cobalt (II) chloride, hexahydrate 16 mg/L EC50
Photobacterium
phosphoreum 15 min as
Co++
160 mg/L EC50
Photobacterium
phosphoreum 5 min as
Co++
2.8 mg/L EC50
Photobacterium
phosphoreum 30 min as
Co++
Components Ecotoxicity - Ecotoxicity - Ecotoxicity - Ecotoxicity -
Fish Species Data Microtox Data Water Flea Data Freshwater Algae Data
Glycerol 50 mg/L LC50 rainbow
trout 96 h
5000 mg/L LC50 goldfish
96 h
13. DISPOSAL CONSIDERATIONS
Waste from residues / unused products: Waste disposal must be in accordance with appropriate Federal, State, and local regulations. This product,
if unaltered by use, may be disposed of by treatment at a permitted facility or as advised by your local
hazardous waste regulatory authority. Residue from fires extinguished with this material may be
hazardous.
14. TRANSPORT INFORMATION
Is product hazardous to ship? Yes.
Component(s) hazardous to ship: Reaction buffer 5x
DOT
Proper shipping name: Arsenic compound, liquid, n.o.s. (Potassium cacodylate solution)
UN Number: UN 1556 Hazard class: 6.1 Subsidiary risk: Not applicable Packing group: III
ICAO/IATA
Proper shipping name: Arsenic compound, liquid, n.o.s. (Potassium cacodylate solution)
UN Number: UN 1556 Hazard class: 6.1 Subsidiary risk: Not applicable Packing group: III
15. REGULATORY INFORMATION
U.S. Regulations:
U.S. CERCLA/SARA/TSCA Regulatory Information: The following chemicals are listed under the following TSCA/SARA/CERCLA lists. Refer to
TSCA regulation if you need a definition for acronyms that may be shown in the TSCA Inventory field in the table below
Components CERCLA/SARA 302 CERCLA/SARA 304 SARA 313 TSCA Inventory
RQ and TPQ RQ Emission reporting
(40 CFR 355, App.A) (40 CFR Table 302.4)
Tris hydrochloride Listed
Components CERCLA/SARA 302 CERCLA/SARA 304 SARA 313 TSCA Inventory
RQ and TPQ RQ Emission reporting
(40 CFR 355, App.A) (40 CFR Table 302.4)
Glycerol Listed
Potassium chloride Listed
U.S. Clean Water Act (CWA)/ California The following chemicals are listed under the CWA and/or California Proposition 65:
Proposition 65:
Product number: 03353583910 Page 4 of 5
Product name: DIG Oligonucleotide Tailing Labeling Kit 2nd generation
Components Clean Water Act Clean Water Act California Proposition 65
Hazardous Substances Priority Pollutants
Cobalt (II) chloride, hexahydrate carcinogen, initial date 7/1/92
(powder)
Canadian Regulations:
Canada DSL Inventory List - This product does not comply with DSL
Canada NDSL Inventory List - This product does not comply with NDSL
Components Canada - WHMIS: Classifications of Substances:
Cobalt (II) chloride, hexahydrate D2A
Components Canada - WHMIS: Classifications of Substances:
Glycerol Uncontrolled product according to WHMIS classification criteria
Potassium chloride Uncontrolled product according to WHMIS classification criteria (including 23.8%)
16. OTHER INFORMATION
Reason for revision: Not applicable
References: None.
Additional advice: None
Prepared by: Roche Diagnostics, Health & Safety Department, MSDS Contact: 317-521-7425 or 317-521-7505
The information, data and recommendations contained herein are based upon information believed by Roche Diagnostics Corporation, after reasonable investigation and
research, to be accurate; however, Roche Diagnostics Corporation does not warrant the accuracy of this information. All materials and mixtures may present unknown
hazards and should be used with caution. When necessary or appropriate, independent opinions regarding the risk of handling or exposure should be obtained from trained
professionals. Roche Diagnostics Corporation disclaims any warranty against patent infringement and the implied warranties of merchantability and fitness for a particular
purpose. Customer's sole and exclusive remedy shall be replacement of the product or return of the product and refund of the purchase price, at Roche Diagnostics
Corporation's option. In no case shall Roche Diagnostics Corporation be liable for incidental or consequential damages, including lost profits.
End of Safety Data Sheet
Product number: 03353583910 Page 5 of 5
Product name: DIG Oligonucleotide Tailing Labeling Kit 2nd generation
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