SIGMA-ALDRICH
Material Safety Data Sheet
Date Printed: 25/NOV/2004
Date Updated: 12/MAR/2004
Version 1.5
According to 91/155/EEC
Classified as Hazardous according to the criteria of EU Annex 1 and
NOHSC.
1 - Product and Company Information
Product Name AURAMINE O, CERTIFIED
Product Number A9655
Company Sigma-Aldrich Pty, Ltd
Unit 2, 14 Anella Avenue
Castle Hill NSW 1765
Technical Phone # +61 2 9841 0555
Fax +61 2 9841 0500
Emergency Phone # +61 2 9841 0566
2 - Composition/Information on Ingredients
Product Name CAS # EC no Annex I
Index Number
AURAMINE O 2465-27-2 219-567-2 None
Formula C17H21N3.ClH
Molecular Weight 303.84 AMU
Synonyms Adc Auramine O * Aizen auramine * Aizen Auramine
Conc. SFA * Aizen auramine OH * Auramine A1 *
Auramine conc. specially soluble in Spirit *
Auramine Extra * Auramine Extra Conc. A *
Auramine FA * Auramine hydrochloride * Auramine
Lake Yellow O * Auramine N * Auramine O *
Auramine O (biological stain) * Auramine O Extra
Conc. A Export * Auramine ON * Auramine OO *
Auramine OOO * Auramine OS * Auramine Pure *
Auramine SP * Auramine Yellow * Benzenamine,
4,4'-carbonimidoylbis(N,N-dimethyl-,
monohydrochloride (9CI) *
4,4'-Bis(dimethylamino)-benzhydrylidenimine
hydrochloride *
4:4'-Bis(dimethylamino)benzophenone-imine
hydrochloride *
1,1-Bis(p-dimethylaminophenyl)methylenimine
hydrochloride * Calcozine Yellow OX * C.I. 41000
* C.I. Basic Yellow 2 * C.I. Basic Yellow 2,
monohydrochloride *
4,4'-(Imidocarbonyl)bis(N,N-dimethylamine),
monohydrochloride * Mitsui auramine O * Zlut
zasadita 2
3 - Hazards Identification
SPECIAL INDICATION OF HAZARDS TO HUMANS AND THE ENVIRONMENT
Harmful if swallowed. Toxic in contact with skin. Limited evidence
of a carcinogenic effect.
4 - First Aid Measures
AFTER INHALATION
If inhaled, remove to fresh air. If not breathing give
artificial respiration. If breathing is difficult, give oxygen.
AFTER SKIN CONTACT
In case of skin contact, flush with copious amounts of water for
at least 15 minutes. Remove contaminated clothing and shoes.
Call a physician.
AFTER EYE CONTACT
In case of contact with eyes, flush with copious amounts of
water for at least 15 minutes. Assure adequate flushing by
separating the eyelids with fingers. Call a physician.
AFTER INGESTION
If swallowed, wash out mouth with water provided person is
conscious. Call a physician immediately.
5 - Fire Fighting Measures
EXTINGUISHING MEDIA
Suitable: Water spray. Carbon dioxide, dry chemical powder, or
appropriate foam.
SPECIAL RISKS
Specific Hazard(s): Emits toxic fumes under fire conditions.
SPECIAL PROTECTIVE EQUIPMENT FOR FIREFIGHTERS
Wear self-contained breathing apparatus and protective clothing
to prevent contact with skin and eyes.
6 - Accidental Release Measures
PERSONAL PRECAUTION PROCEDURES TO BE FOLLOWED IN CASE OF LEAK OR SPILL
Evacuate area.
PROCEDURE(S) OF PERSONAL PRECAUTION(S)
Wear self-contained breathing apparatus, rubber boots, and heavy
rubber gloves.
METHODS FOR CLEANING UP
Sweep up, place in a bag and hold for waste disposal. Avoid
raising dust. Ventilate area and wash spill site after material
pickup is complete.
7 - Handling and Storage
HANDLING
Directions for Safe Handling: Do not breathe dust. Do not get in
eyes, on skin, on clothing. Avoid prolonged or repeated exposure.
STORAGE
Conditions of Storage: Keep tightly closed.
8 - Exposure Controls / Personal Protection
ENGINEERING CONTROLS
Use only in a chemical fume hood. Safety shower and eye bath.
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GENERAL HYGIENE MEASURES
Wash contaminated clothing before reuse. Wash thoroughly after
handling.
PERSONAL PROTECTIVE EQUIPMENT
Respiratory Protection: Government approved respirator.
Hand Protection: Compatible chemical-resistant gloves.
Eye Protection: Chemical safety goggles.
9 - Physical and Chemical Properties
Appearance Physical State: Solid
Color: Deep yellow
Form: Powder
Property Value At Temperature or Pressure
pH 6.5 20 藲C Concentration: 10
g/l
BP/BP Range N/A
MP/MP Range 250 藲C
Flash Point N/A
Flammability N/A
Autoignition Temp N/A
Oxidizing Properties N/A
Explosive Properties N/A
Explosion Limits N/A
Vapor Pressure N/A
SG/Density N/A
Partition Coefficient N/A
Viscosity N/A
Vapor Density N/A
Saturated Vapor Conc. N/A
Evaporation Rate N/A
Bulk Density N/A
Decomposition Temp. N/A
Solvent Content N/A
Water Content N/A
Surface Tension N/A
Conductivity N/A
Miscellaneous Data N/A
Solubility Solubility in Water:Soluble. 10 mg/ml H2O,
20藲C
10 - Stability and Reactivity
STABILITY
Stable: Stable.
Materials to Avoid: Strong oxidizing agents.
HAZARDOUS DECOMPOSITION PRODUCTS
Hazardous Decomposition Products: Carbon monoxide, Carbon dioxide,
Nitrogen oxides, Hydrogen chloride gas.
HAZARDOUS POLYMERIZATION
Hazardous Polymerization: Will not occur
11 - Toxicological Information
RTECS NUMBER: BY3675000
ACUTE TOXICITY
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LD50
Intraperitoneal
Rat
135 MG/KG
LD50
Oral
Mouse
480 mg/kg
LD50
Skin
Mouse
300 mg/kg
LD50
Subcutaneous
Mouse
300 MG/KG
LD50
Oral
Cat
150 mg/kg
LD50
Oral
Domestic Animals
150 mg/kg
ROUTE OF EXPOSURE
Skin Contact: May cause skin irritation.
Skin Absorption: Toxic if absorbed through skin.
Eye Contact: May cause eye irritation.
Inhalation: May be harmful if inhaled. Material may be
irritating to mucous membranes and upper respiratory tract.
Ingestion: Harmful if swallowed.
CHRONIC EXPOSURE - CARCINOGEN
Rat
Route of Application: Oral
Exposure Time: 87W
Result: Tumorigenic:Neoplastic by RTECS criteria. Liver:Tumors.
Kidney, Ureter, Bladder:Tumors.
Rat
Route of Application: Subcutaneous
Exposure Time: 21W
Result: Tumorigenic:Equivocal tumorigenic agent by RTECS
criteria. Liver:Tumors. Tumorigenic:Tumors at site or
application.
Mouse
Route of Application: Oral
Exposure Time: 52W
Result: Tumorigenic:Neoplastic by RTECS criteria. Liver:Tumors.
Rat
Route of Application: Oral
Exposure Time: 39W
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Result: Tumorigenic:Equivocal tumorigenic agent by RTECS
criteria. Liver:Tumors.
CHRONIC EXPOSURE - MUTAGEN
Human
18 UMOL/L
Cell Type: liver
DNA damage
Human
300 UMOL/L
Cell Type: fibroblast
DNA damage
Human
62 UMOL/L
Cell Type: HeLa cell
DNA inhibition
Rat
4914 MG/KG
Oral
Morphological transformation.
Rat
9 MG/KG
Intraperitoneal
DNA damage
Rat
3 UMOL/L
Cell Type: liver
DNA damage
Rat
125 MG/KG
Oral
DNA damage
Mouse
15 MG/KG
Intraperitoneal
DNA damage
Mouse
7500 UG/KG
Intraperitoneal
Sister chromatid exchange
Hamster
20 UMOL/L
5H
Cell Type: ovary
Cytogenetic analysis
12 - Ecological Information
No data available.
13 - Disposal Considerations
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SUBSTANCE DISPOSAL
Contact a licensed professional waste disposal service to dispose
of this material. Dissolve or mix the material with a combustible
solvent and burn in a chemical incinerator equipped with an
afterburner and scrubber. Observe all federal, state, and local
environmental regulations.
14 - Transport Information
RID/ADR
UN#: 2811
Class: 6.1
PG: III
Proper Shipping Name: Toxic solid, organic, n.o.s.
IMDG
UN#: 2811
Class: 6.1
PG: III
Proper Shipping Name: Toxic solid, organic, n.o.s.
Marine Pollutant: No
Severe Marine Pollutant: No
Technical Name: Required
IATA
UN#: 2811
Class: 6.1
PG: III
Proper Shipping Name: Toxic solid, organic, n.o.s.
Inhalation Packing Group I: No
Technical Name: Required
15 - Regulatory Information
CLASSIFICATION AND LABELING ACCORDING TO EU DIRECTIVES
INDICATION OF DANGER: T
Toxic.
R-PHRASES: 22 24 40
Harmful if swallowed. Toxic in contact with skin. Limited
evidence of a carcinogenic effect.
S-PHRASES: 36/37 45
Wear suitable protective clothing and gloves. In case of
accident or if you feel unwell, seek medical advice immediately
(show the label where possible).
SWITZERLAND
SWISS POISON CLASS: 1*
16 - Other Information
WARRANTY
The above information is believed to be correct but does not
purport to be all inclusive and shall be used only as a guide. The
information in this document is based on the present state of our
knowledge and is applicable to the product with regard to
appropriate safety precautions. It does not represent any
guarantee of the properties of the product. Sigma-Aldrich Inc.,
shall not be held liable for any damage resulting from handling or
from contact with the above product. See reverse side of invoice
or packing slip for additional terms and conditions of sale.
Copyright 2004 Sigma-Aldrich Co. License granted to make unlimited
paper copies for internal use only.
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DISCLAIMER
For R&D use only. Not for drug, household or other uses.
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