SIGMA-ALDRICH
MATERIAL SAFETY DATA SHEET
Date Printed: 02/01/2006
Date Updated: 04/06/2004
Version 1.4
Section 1 - Product and Company Information
Product Name BOTULINUM TOXIN B FROM CLOSTRIDIUM
BOTULINUM
Product Number B6403
Brand SIGMA
Company Sigma-Aldrich
Street Address 3050 Spruce Street
City, State, Zip, Country SAINT LOUIS MO 63103 US
Technical Phone: 800-325-5832
Emergency Phone: 314-776-6555
Fax: 800-325-5052
Section 2 - Composition/Information on Ingredient
Substance Name CAS # SARA 313
BOTULINUM TOXIN B FROM 93384-44-2 No
CLOSTRIDIUMBOTULINUM
Ingredient Name CAS # Percent SARA 313
BOTULINUM TOXIN SUBUNIT None 0.1
SODIUM CHLORIDE 7647-14-5 1.17 No
SODIUM ACETATE, ANHYDROUS 127-09-3 0.41 No
Chemical Family Etiological agent.
Synonyms Botulin toxin B * BOTULIN NEUROTOXIN B * BORALIN
TOXIN B * TOXIN BOTULIN B
RTECS Number: ED9250000
Section 3 - Hazards Identification
EMERGENCY OVERVIEW
Biohazard. Highly Toxic (USA) Very Toxic (EU).
Very toxic by inhalation, in contact with skin and if swallowed.
Irritating to eyes and skin.
Biomedical material. May cause human disease. Target organ(s):
Nerves.
HMIS RATING
HEALTH: 4
FLAMMABILITY: 0
REACTIVITY: 1
NFPA RATING
HEALTH: 4
FLAMMABILITY: 0
REACTIVITY: 1
For additional information on toxicity, please refer to Section 11.
Section 4 - First Aid Measures
ORAL EXPOSURE
If swallowed, wash out mouth with water provided person is
conscious. Call a physician immediately.
INHALATION EXPOSURE
If inhaled, remove to fresh air. If not breathing give
artificial respiration. If breathing is difficult, give oxygen.
DERMAL EXPOSURE
In case of skin contact, flush with copious amounts of water for
at least 15 minutes. Remove contaminated clothing and shoes.
Call a physician.
EYE EXPOSURE
In case of contact with eyes, flush with copious amounts of
water for at least 15 minutes. Assure adequate flushing by
separating the eyelids with fingers. Call a physician.
INFORMATION FOR PHYSICIAN
In certain emergency situations, the Center for Disease Control
can supply botulinum toxoid, pentavalent (abcde) an
investigational new drug. These biological products are
available through the Immunobiologics Bureau of Laboratories,
Center for Disease Control in Atlanta, Georgia USA. Phone
requests may be made to CDC at USA. 404-639-3311 from 8:00 am to
4:30 PM Monday through Friday. After working hours or on
weekends and holidays call USA 404-639-2888. Advise the
physician of the compound to which the person was exposed.
Section 5 - Fire Fighting Measures
FLASH POINT
N/A
AUTOIGNITION TEMP
N/A
FLAMMABILITY
N/A
EXTINGUISHING MEDIA
Suitable: Water spray. Carbon dioxide, dry chemical powder, or
appropriate foam.
FIREFIGHTING
Protective Equipment: Wear self-contained breathing apparatus
and protective clothing to prevent contact with skin and eyes.
Specific Hazard(s): Emits toxic fumes under fire conditions.
Section 6 - Accidental Release Measures
PROCEDURE TO BE FOLLOWED IN CASE OF LEAK OR SPILL
Evacuate area.
PROCEDURE(S) OF PERSONAL PRECAUTION(S)
Wear self-contained breathing apparatus, rubber boots, and heavy
rubber gloves.
METHODS FOR CLEANING UP
Sweep up, place in a bag and hold for waste disposal. Avoid
raising dust. Ventilate area and wash spill site after material
pickup is complete.
SIGMA - B6403 www.sigma-aldrich.com Page 2
Section 7 - Handling and Storage
HANDLING
User Exposure: Botulinum toxin a should be handled in a closed
system. All operations should be carried out in a glove bag of
similar enclosure to avoid accidental contact. Container should
be opened only by a technically qualified person. Do not use if
skin is cut or scratched. Wash thoroughly after handling. Handle
as if capable of transmitting infectious agents.
STORAGE
Suitable: Keep tightly closed.
Store at -20癈
SPECIAL REQUIREMENTS
Light sensitive. Heat sensitive.
Section 8 - Exposure Controls / PPE
ENGINEERING CONTROLS
Use only in a chemical fume hood. Safety shower and eye bath.
PERSONAL PROTECTIVE EQUIPMENT
Respiratory: Use respirators and components tested and approved
under appropriate government standards such as NIOSH (US) or CEN
(EU). Where risk assessment shows air-purifying respirators are
appropriate use a full-face particle respirator type N100 (US) or
type P3 (EN 143) respirator cartridges as a backup to engineering
controls. If the respirator is the sole means of protection, use a
full-face supplied air respirator.
Hand: Compatible chemical-resistant gloves.
Eye: Chemical safety goggles.
GENERAL HYGIENE MEASURES
Wash contaminated clothing before reuse. Wash thoroughly after
handling.
Section 9 - Physical/Chemical Properties
Appearance Physical State: Liquid
Property Value At Temperature or Pressure
Molecular Weight 500,000 AMU
pH N/A
BP/BP Range N/A
MP/MP Range N/A
Freezing Point N/A
Vapor Pressure N/A
Vapor Density N/A
Saturated Vapor Conc. N/A
SG/Density N/A
Bulk Density N/A
Odor Threshold N/A
Volatile% N/A
VOC Content N/A
Water Content N/A
Solvent Content N/A
Evaporation Rate N/A
Viscosity N/A
Surface Tension N/A
SIGMA - B6403 www.sigma-aldrich.com Page 3
Partition Coefficient N/A
Decomposition Temp. N/A
Flash Point N/A
Explosion Limits N/A
Flammability N/A
Autoignition Temp N/A
Refractive Index N/A
Optical Rotation N/A
Miscellaneous Data N/A
Solubility N/A
N/A = not available
Section 10 - Stability and Reactivity
STABILITY
Stable: Stable.
Conditions to Avoid: Direct sunlight Heat.
Materials to Avoid: Bases.
HAZARDOUS DECOMPOSITION PRODUCTS
Hazardous Decomposition Products: Carbon monoxide, Carbon dioxide.
HAZARDOUS POLYMERIZATION
Hazardous Polymerization: Will not occur
Section 11 - Toxicological Information
ROUTE OF EXPOSURE
Skin Contact: Causes skin irritation.
Skin Absorption: May be fatal if absorbed through skin.
Eye Contact: Causes eye irritation.
Inhalation: Material may be irritating to mucous membranes and
upper respiratory tract. May be fatal if inhaled.
Ingestion: May be fatal if swallowed.
SIGNS AND SYMPTOMS OF EXPOSURE
This toxin is among the most powerful paralytic poisons known,
having irreversible effects. Considered a lethal neurotoxin, 1ug
may be fatal if swallowed or inhaled. Orl-man LD50:1ug/man
(Microbial Toxins, Vol. IIa,1971). Botulinum toxin acts
principally by paralyzing the synapses (junctions) of the
peripheral nerves leading to muscles. Physiological changes
include nausea, vertigo, pharyngeal pain, blurred vision,
constipation, and respiratory paralysis. Type E toxin acts more
slowly than type A, which has a rapid time of onset. After
ingestion of a sufficient dose of type E toxin, humans show
symptoms in 4 to 6 hours, and death occurs in several days.
(Frontier 26, (2), 17 ,1965).
Section 12 - Ecological Information
No data available.
Section 13 - Disposal Considerations
APPROPRIATE METHOD OF DISPOSAL OF SUBSTANCE OR PREPARATION
After use, utensils and the toxin solution should be soaked
overnight or boiled for 20 minutes in an excess amount of 2%
sodium hydroxide solution or sodium hypochlorite (1% available
chlorine) solution in a hood or well ventilated area. Dissolve or
mix the material with a combustible solvent and burn in a chemical
SIGMA - B6403 www.sigma-aldrich.com Page 4
incinerator equipped with an afterburner and scrubber. Observe all
federal, state, and local environmental regulations.
Section 14 - Transport Information
DOT
Proper Shipping Name: Toxins, from living sources,
liquid, n.o.s.
UN#: 3172
Class: 6.1
Packing Group: Packing Group I
Hazard Label: Toxic substances.
PIH: Not PIH
IATA
Proper Shipping Name: Toxins, extracted from living
sources, liquid, n.o.s.
IATA UN Number: 3172
Hazard Class: 6.1
Packing Group: I
Section 15 - Regulatory Information
EU ADDITIONAL CLASSIFICATION
Symbol of Danger: B-T+
Indication of Danger: Biohazard. Very toxic.
R: 26/27/28-36/38
Risk Statements: Very toxic by inhalation, in contact with skin
and if swallowed. Irritating to eyes and skin.
S: 53-45
Safety Statements: Restricted to professional users. Attention -
Avoid exposure - obtain special instructions before use. In case
of accident or if you feel unwell, seek medical advice
immediately (show the label where possible).
US CLASSIFICATION AND LABEL TEXT
Indication of Danger: Biohazard. Highly Toxic (USA) Very Toxic
(EU).
Risk Statements: Very toxic by inhalation, in contact with skin
and if swallowed. Irritating to eyes and skin.
Safety Statements: Restricted to professional users. Attention -
Avoid exposure - obtain special instructions before use. In case
of accident or if you feel unwell, seek medical advice
immediately (show the label where possible).
US Statements: Biomedical material. May cause human disease.
Target organ(s): Nerves.
UNITED STATES REGULATORY INFORMATION
SARA LISTED: No
CANADA REGULATORY INFORMATION
WHMIS Classification: This product has been classified in
accordance with the hazard criteria of the CPR, and the MSDS
contains all the information required by the CPR.
DSL: No
NDSL: No
Section 16 - Other Information
DISCLAIMER
For R&D use only. Not for drug, household or other uses.
SIGMA - B6403 www.sigma-aldrich.com Page 5
WARRANTY
The above information is believed to be correct but does not
purport to be all inclusive and shall be used only as a guide. The
information in this document is based on the present state of our
knowledge and is applicable to the product with regard to
appropriate safety precautions. It does not represent any
guarantee of the properties of the product. Sigma-Aldrich Inc.,
shall not be held liable for any damage resulting from handling or
from contact with the above product. See reverse side of invoice
or packing slip for additional terms and conditions of sale.
Copyright 2006 Sigma-Aldrich Co. License granted to make unlimited
paper copies for internal use only.
SIGMA - B6403 www.sigma-aldrich.com Page 6
|