Page 1 of 16
@Beckman
CoulterTM Kit MSDS
Doc. ID: IM3614-75: Rev. AA
Product Information
ANNEXIN V FITC / 7-AAD KIT 150
Product Name
Test Doc. ID IM3614-75 AA
Issued (year/month/day) 2002/01/16
Part Number IM3614
Series Name:
Components
Description
Annexin V-FITC
10x Concentrated Binding Buffer
7-AAD Viability Dye
Transport Information
Transportation of this product is not regulated under ICAO, IATA, US DOT, European ADR or Canadian TDG.
?2002 Beckman Coulter, Inc.
Global MSDS ? English
Page 2 of 16
@Beckman
CoulterTM MATERIAL SAFETY DATA SHEET
Section 1 Company and Product Identification
Annexin V-FITC
Product Name ANNEXIN V FITC / 7-AAD KIT 150 Test
Doc. ID IM3614-75 AA
Issued (year/month/day) 2002/01/16
Part Number Component of P/N IM3614
Manufacturer Distributed by Beckman Coulter, Inc. for
IMMUNOTECH (A Beckman Coulter Company)
130, av.de Lattre de Tassigny—BP 177
13276 Marseille Cedex 09—France Tèl. (33) 4 91 17 27 00
¤
Distributor and Refer to attached list, Document ID: 472050, for local distributor and
Emergency Phone No. emergency phone numbers.
Section 2 Composition and Information on Ingredients
Hazardous Ingredients: None
Section 3 Hazards Identification
Emergency Overview
Colorless; Clear; Liquid; Odorless
Nonflammable aqueous solution.
Does not meet EU, OSHA or WHMIS criteria for hazardous materials.
Contains material of animal origin.
Physical Hazards No physical hazards were determined from a review of available literature.
Potential Health This product does not meet EU, OSHA or WHMIS criteria for hazardous materials.
Effects Summary However, it does contain material of animal origin and should be considered
potentially infectious.
Product Hazard EU: WHMIS: US OSHA:
Classifications Not applicable Exempt Not applicable
Beckman Coulter Safety Flammability (Section V): 0 Code
Rating Health (Section XI): 1 0=none
Reactivity with Water (Section X): 0 1=slight
Contact (Section VIII): 1 2=caution
3=severe
Section 4 First Aid Measures
Inhalation If product is inhaled, move exposed individual to fresh air. If individual is not
breathing, begin artificial respiration immediately and obtain medical attention.
Eye Contact If product enters eyes, wash eyes gently under running water for 15 minutes or
longer, making sure that the eyelids are held open. If pain or irritation occur, obtain
medical attention.
Skin Contact Remove any contaminated clothing. Wash affected area with plenty of soap and
water. Use disinfectant if possible.
ANNEXIN V FITC / 7-AAD KIT 150 Test Doc. ID: IM3614-75 AA Page 3 of 16
Annexin V-FITC
Section 4 First Aid Measures (Continued)
Ingestion If ingested, wash mouth out with water. If irritation or discomfort occurs, seek medical
attention.
Section 5 Fire Fighting Measures
Flash Point Not applicable
Flammable Limits Not applicable
Autoignition Temp. Not applicable
Extinguishing Media Use extinguishing media suitable for surrounding fire.
Special Fire and None identified.
Explosion Hazards
Hazardous Combustion No combustion products posing significant hazards are expected from this product (a
Products dilute aqueous solution).
Protective Equipment Self-contained breathing apparatus is recommended for firefighters.
for Firefighters
Section 6 Accidental Release Measures
Personal Precautions This product contains a material of biological origin. Use universal precautions during
clean up procedures.
Spill and Leak As a precautionary measure, treat spilled material with a 1:10 bleach/water solution.
Procedures Absorb liquid and place in container suitable for disposal. Avoid generation of
aerosols during clean up. Comply with applicable waste disposal regulations.
Environmental Contain spill to prevent migration.
Precautions
Section 7 Handling and Storage
Handling Precautions This product should be handled as though capable of transmitting infectious diseases.
Universal precautions should be followed when using this product.
Recommended Storage Keep away from incompatible material.
Conditions To maintain efficacy, store according to the instructions in the product labeling.
Section 8 Exposure Controls and Personal Protection
Exposure Limits
US OSHA: None established
ACGIH: None established
DFG MAK: None established
Engineering Controls No special engineering controls are required. Use with good general ventilation.
Respiratory Protection Under normal conditions, the use of this product should not require respiratory
protection.
Eye Protection Safety glasses or chemical goggles should be worn to prevent eye contact.
ANNEXIN V FITC / 7-AAD KIT 150 Test Doc. ID: IM3614-75 AA Page 4 of 16
Annexin V-FITC
Section 8 Exposure Controls and Personal Protection (Continued)
Skin Protection Impervious gloves, such as latex or equivalent, should be worn to prevent skin
contact.
Section 9 Physical and Chemical Properties
Physical State Liquid
Color Colorless
Transparency Clear
Odor Odorless
Odor Threshold Not applicable
pH 7.4
Boiling Point Not available
Melting Point Not available
Specific Gravity 1.01 @20°C
Vapor Pressure Not available
Vapor Density Not available
Evaporation Rate Not available
Solubility
Water Miscible
Organic Not available
Section 10 Stability and Reactivity
Stability Stable in closed containers under normal temperatures and pressures.
Hazardous Strong acids
Incompatibilities Strong bases
Hazardous No decomposition products posing significant hazards would be expected from this
Decomposition Products product (a dilute aqueous solution).
Conditions to Avoid Avoid contact with incompatible materials.
Section 11 Toxicological Information
Toxicity Data Not applicable
for Hazardous Ingredients
Primary Routes Common routes of entry include inhalation, ingestion and eye/skin contact. Specific
of Exposure paths of concern for potentially infectious materials are skin puncture, contact with
broken skin, contact with mucous membranes and inhalation of aerosolized material.
Potential Effects of This product contains materials of animal origin and should be considered potentially
Acute Exposure infectious. No other health hazards were identified from a review of available
literature.
Potential Effects of Effects are similar to those for acute exposure.
Chronic Exposure
ANNEXIN V FITC / 7-AAD KIT 150 Test Doc. ID: IM3614-75 AA Page 5 of 16
Annexin V-FITC
Section 11 Toxicological Information (Continued)
Symptoms of No specific symptoms identified.
Overexposure
Carcinogenicity No ingredients in this product are listed as carcinogens by ACGIH, IARC, NTP, OSHA
or 67/548/EEC Annex I.
Other Effects None identified.
Conditions Aggravated None identified.
by Exposure
Section 12 Ecological Information
Ecotoxicity No information available.
Biodegradability No information available.
Mobility No information available.
Section 13 Disposal Considerations
Waste Disposal Dispose of waste product, unused product and contaminated packaging in compliance
with federal, state and local regulations. If unsure of the applicable requirements,
contact the authorities for information.
Section 14 Transport Information
Transportation of this product is not regulated under ICAO, IATA, US DOT, European ADR or Canadian TDG.
Section 15 Regulatory Information
US Federal and State Regulations
SARA 313 No ingredients listed
CERCLA RG’s, Edetic Acid is listed.
40 CFR 302.4 Sodium Hydroxide is listed.
California Proposition 65 No ingredients listed
Massachusetts MSL Edetic Acid is listed.
Sodium Hydroxide is listed.
New Jersey Dept. of Edetic Acid is listed.
Health RTK List Sodium Hydroxide is listed.
Pennsylvania RTK Edetic Acid is listed.
Sodium Hydroxide is listed.
Florida Substance List Sodium Hydroxide is listed.
EU Labeling Classification
Preparation not classified.
ANNEXIN V FITC / 7-AAD KIT 150 Test Doc. ID: IM3614-75 AA Page 6 of 16
Annexin V-FITC
Section 15 Regulatory Information (Continued)
Canada
This product is exempt from WHMIS label and MSDS requirements.
PIN: Not applicable
Ingredients on Ingredient Sodium Hydroxide
Disclosure List:
Ingredients with unknown Product is exempt
toxicological properties:
Section 16 Other Information
For further information, please contact your local Beckman Coulter representative.
WHILE BECKMAN COULTER, INC. BELIEVES THE INFORMATION CONTAINED HEREIN IS VALID AND ACCURATE, BECKMAN COULTER MAKES NO WARRANTY OR
REPRESENTATION AS TO ITS VALIDITY, ACCURACY, OR CURRENCY. BECKMAN COULTER SHALL NOT BE LIABLE OR OTHERWISE RESPONSIBLE IN ANY WAY FOR USE
OF EITHER THIS INFORMATION OR MATERIALS TO WHICH IT APPLIES. DISPOSAL OF HAZARDOUS MATERIALS MAY BE SUBJECT TO LOCAL LAWS OR REGULATIONS.
Printed in U.S.A.
Page 7 of 16
@Beckman
CoulterTM MATERIAL SAFETY DATA SHEET
Section 1 Company and Product Identification
10x Concentrated
Product Name
Binding Buffer ANNEXIN V FITC / 7-AAD KIT 150 Test
Doc. ID IM3614-75 AA
Issued (year/month/day) 2002/01/16
Part Number Component of P/N IM3614
Manufacturer Distributed by Beckman Coulter, Inc. for
IMMUNOTECH (A Beckman Coulter Company)
130, av.de Lattre de Tassigny—BP 177
13276 Marseille Cedex 09—France Tèl. (33) 4 91 17 27 00
¤
Distributor and Refer to attached list, Document ID: 472050, for local distributor and
Emergency Phone No. emergency phone numbers.
Section 2 Composition and Information on Ingredients
Hazardous Ingredients: None
Section 3 Hazards Identification
Emergency Overview
Colorless; Clear; Liquid; Odorless
Nonflammable aqueous solution.
Does not meet EU, OSHA or WHMIS criteria for hazardous materials.
Physical Hazards No physical hazards were determined from a review of available literature.
Potential Health This product does not meet EU, OSHA or WHMIS criteria for hazardous materials.
Effects Summary
Product Hazard EU: WHMIS: US OSHA:
Classifications Not applicable Exempt Not applicable
Beckman Coulter Safety Flammability (Section V): 0 Code
Rating Health (Section XI): 0 0=none
Reactivity with Water (Section X): 0 1=slight
Contact (Section VIII): 1 2=caution
3=severe
Section 4 First Aid Measures
Inhalation If product is inhaled, move exposed individual to fresh air. If individual is not
breathing, begin artificial respiration immediately and obtain medical attention.
Eye Contact If product enters eyes, wash eyes gently under running water for 15 minutes or
longer, making sure that the eyelids are held open. If pain or irritation occur, obtain
medical attention.
Skin Contact In case of skin contact, flush with copious amounts of water for at least 15 minutes. If
pain or irritation occur, obtain medical attention.
ANNEXIN V FITC / 7-AAD KIT 150 Test Doc. ID: IM3614-75 AA Page 8 of 16
10x Concentrated Binding Buffer
Section 4 First Aid Measures (Continued)
Ingestion If ingested, wash mouth out with water. If irritation or discomfort occurs, seek medical
attention.
Section 5 Fire Fighting Measures
Flash Point Not applicable
Flammable Limits Not applicable
Autoignition Temp. Not applicable
Extinguishing Media Use extinguishing media suitable for surrounding fire.
Special Fire and No special hazards determined.
Explosion Hazards
Hazardous Combustion No combustion products posing significant hazards are expected from this product (a
Products dilute aqueous solution).
Protective Equipment Self-contained breathing apparatus is recommended for firefighters in all chemical fire
for Firefighters situations.
Section 6 Accidental Release Measures
Personal Precautions No special precautions are necessary. Use good laboratory procedures.
Spill and Leak Wipe up spill and place in container suitable for disposal.
Procedures
Environmental Contain spill to prevent migration.
Precautions
Section 7 Handling and Storage
Handling Precautions No special precautions are necessary; use good laboratory procedures.
Recommended Storage Keep in the original container at room temperature.
Conditions
Section 8 Exposure Controls and Personal Protection
Exposure Limits
US OSHA: None established
ACGIH: None established
DFG MAK: None established
Engineering Controls No special engineering controls are required. Use with good general ventilation.
Respiratory Protection Under normal conditions, the use of this product should not require respiratory
protection.
Eye Protection Safety glasses are recommended to prevent eye contact.
Skin Protection Impervious gloves, such as latex or equivalent, should be worn to prevent skin
contact.
ANNEXIN V FITC / 7-AAD KIT 150 Test Doc. ID: IM3614-75 AA Page 9 of 16
10x Concentrated Binding Buffer
Section 9 Physical and Chemical Properties
Physical State Liquid
Color Colorless
Transparency Clear
Odor Odorless
Odor Threshold Not applicable
pH 7.4
Boiling Point Not available
Melting Point Not available
Specific Gravity 1.01 @20°C
Vapor Pressure Not available
Vapor Density Not available
Evaporation Rate Not available
Solubility
Water Miscible
Organic Not available
Section 10 Stability and Reactivity
Stability Stable under normal temperatures and pressures.
Hazardous No hazardous incompatibilities were determined from a review of literature available.
Incompatibilities
Hazardous No decomposition products posing significant hazards would be expected from this
Decomposition Products product (a dilute aqueous solution).
Conditions to Avoid None determined.
Section 11 Toxicological Information
Toxicity Data Not applicable
for Hazardous Ingredients
Primary Routes The most likely routes of exposure are skin and eye contact.
of Exposure
Potential Effects of None identified.
Acute Exposure
Potential Effects of None identified.
Chronic Exposure
Symptoms of None identified.
Overexposure
Carcinogenicity No ingredients in this product are listed as carcinogens by ACGIH, IARC, NTP, OSHA
or 67/548/EEC Annex I.
Other Effects None identified.
ANNEXIN V FITC / 7-AAD KIT 150 Test Doc. ID: IM3614-75 AA Page 10 of 16
10x Concentrated Binding Buffer
Section 11 Toxicological Information (Continued)
Conditions Aggravated None identified.
by Exposure
Section 12 Ecological Information
Ecotoxicity No information available.
Biodegradability No information available.
Mobility No information available.
Section 13 Disposal Considerations
Waste Disposal Dispose of waste product, unused product and contaminated packaging in compliance
with federal, state and local regulations. If unsure of the applicable requirements,
contact the authorities for information.
Section 14 Transport Information
Transportation of this product is not regulated under ICAO, IATA, US DOT, European ADR or Canadian TDG.
Section 15 Regulatory Information
US Federal and State Regulations
SARA 313 No ingredients listed
CERCLA RG’s, No ingredients listed
40 CFR 302.4
California Proposition 65 No ingredients listed
Massachusetts MSL Magnesium Chloride is listed.
New Jersey Dept. of Magnesium Chloride is listed.
Health RTK List
Pennsylvania RTK Magnesium Chloride is listed.
Florida Substance List Magnesium Chloride is listed.
EU Labeling Classification
Preparation not classified.
Canada
This product is exempt from WHMIS label and MSDS requirements.
PIN: Not applicable
Ingredients on Ingredient None
Disclosure List:
Ingredients with unknown Product is exempt
toxicological properties:
ANNEXIN V FITC / 7-AAD KIT 150 Test Doc. ID: IM3614-75 AA Page 11 of 16
10x Concentrated Binding Buffer
Section 16 Other Information
For further information, please contact your local Beckman Coulter representative.
WHILE BECKMAN COULTER, INC. BELIEVES THE INFORMATION CONTAINED HEREIN IS VALID AND ACCURATE, BECKMAN COULTER MAKES NO WARRANTY OR
REPRESENTATION AS TO ITS VALIDITY, ACCURACY, OR CURRENCY. BECKMAN COULTER SHALL NOT BE LIABLE OR OTHERWISE RESPONSIBLE IN ANY WAY FOR USE
OF EITHER THIS INFORMATION OR MATERIALS TO WHICH IT APPLIES. DISPOSAL OF HAZARDOUS MATERIALS MAY BE SUBJECT TO LOCAL LAWS OR REGULATIONS.
Printed in U.S.A.
Page 12 of 16
@Beckman
CoulterTM MATERIAL SAFETY DATA SHEET
Section 1 Company and Product Identification
7-AAD Viability Dye
Product Name
ANNEXIN V FITC / 7-AAD KIT 150 Test
Doc. ID IM3614-75 AA
Issued (year/month/day) 2002/01/16
Part Number Component of P/N IM3614
Manufacturer Distributed by Beckman Coulter, Inc. for
IMMUNOTECH (A Beckman Coulter Company)
130, av.de Lattre de Tassigny—BP 177
13276 Marseille Cedex 09—France Tèl. (33) 4 91 17 27 00
¤
Distributor and Refer to attached list, Document ID: 472050, for local distributor and
Emergency Phone No. emergency phone numbers.
Section 2 Composition and Information on Ingredients
Hazardous Ingredients: Meets Hazardous Criteria:
Chemical Name CAS # % by wt. EU US OSHA WHMIS
Dimethyl Sulfoxide 67-68-5 1.0 No Yes Exempt
See Section 15 Regulatory Information for additional information on hazard classifications.
Section 3 Hazards Identification
Emergency Overview
Violet; Clear; Liquid; Odorless
Nonflammable aqueous solution.
Skin, eye and respiratory tract irritant.
This product contains 0.005% 7-Aminoactinomycin (CAS# 7240-37-1). Although
not considered hazardous at this concentration, pure 7-AAD is classified as T+;
R26/27/28-45-46-61 within the meaning of European Directive 88/379/EEC.
Physical Hazards No physical hazards were determined from a review of available literature.
Potential Health Exposure may result in irritation of skin, eyes, and mucous membranes.
Effects Summary
See Section 11 Toxicological Information for more detailed health information.
Product Hazard EU: WHMIS: US OSHA:
Classifications Not applicable Exempt Hazardous
Beckman Coulter Safety Flammability (Section V): 0 Code
Rating Health (Section XI): 1 0=none
Reactivity with Water (Section X): 0 1=slight
Contact (Section VIII): 2 2=caution
3=severe
ANNEXIN V FITC / 7-AAD KIT 150 Test Doc. ID: IM3614-75 AA Page 13 of 16
7-AAD Viability Dye
Section 4 First Aid Measures
Inhalation If product is inhaled, move exposed individual to fresh air. If individual is not
breathing, begin artificial respiration immediately and obtain medical attention.
Eye Contact If product enters eyes, wash eyes gently under running water for 15 minutes or
longer, making sure that the eyelids are held open. If pain or irritation occur, obtain
medical attention.
Skin Contact In case of skin contact, flush with copious amounts of water for at least 15 minutes. If
pain or irritation occur, obtain medical attention.
Ingestion If ingested, wash mouth out with water. If irritation or discomfort occurs, seek medical
attention.
Section 5 Fire Fighting Measures
Flash Point Not applicable
Flammable Limits Not applicable
Autoignition Temp. Not applicable
Extinguishing Media Use extinguishing media suitable for surrounding fire.
Special Fire and No special hazards determined.
Explosion Hazards
Hazardous Combustion No combustion products posing significant hazards are expected from this product (a
Products dilute aqueous solution).
Protective Equipment Self-contained breathing apparatus is recommended for firefighters.
for Firefighters
Section 6 Accidental Release Measures
Personal Precautions No special precautions are necessary. Use good laboratory procedures.
Spill and Leak Absorb spilled material with an appropriate inert, non-flammable absorbent and
Procedures dispose according to local regulations.
Environmental Contain spill to prevent migration.
Precautions
Section 7 Handling and Storage
Handling Precautions Use good laboratory procedures; avoid eye and skin contact.
Recommended Storage Keep away from incompatible material.
Conditions To maintain efficacy, store according to the instructions in the product labeling.
Section 8 Exposure Controls and Personal Protection
Exposure Limits
US OSHA: None established
ACGIH: None established
ANNEXIN V FITC / 7-AAD KIT 150 Test Doc. ID: IM3614-75 AA Page 14 of 16
7-AAD Viability Dye
Section 8 Exposure Controls and Personal Protection (Continued)
DFG MAK:
Dimethyl Sulfoxide danger of cutaneous absorption
Engineering Controls No special engineering controls are required. Use with good general ventilation.
Respiratory Protection Under normal conditions, the use of this product should not require respiratory
protection. If overexposure should occur and ventilation is not adequate to maintain
airborne concentrations at acceptable levels, the use of respiratory protection should
be evaluated by a qualified professional.
Eye Protection Safety glasses or chemical goggles should be worn to prevent eye contact.
Skin Protection Impervious gloves, such as latex or equivalent, should be worn to prevent skin
contact.
Section 9 Physical and Chemical Properties
Physical State Liquid
Color Violet
Transparency Clear
Odor Odorless
Odor Threshold Not applicable
pH 7
Boiling Point Not available
Melting Point Not available
Specific Gravity 1 @20°C
Vapor Pressure Not available
Vapor Density Not available
Evaporation Rate Not available
Solubility
Water Miscible
Organic Not available
Section 10 Stability and Reactivity
Stability Stable under normal temperatures and pressures.
Hazardous Strong oxidizers
Incompatibilities Strong acids
Strong bases
Hazardous When stored as labeled, no known hazardous decomposition products are formed
Decomposition Products during the shelf-life of this product.
Conditions to Avoid Keep away from incompatible material.
ANNEXIN V FITC / 7-AAD KIT 150 Test Doc. ID: IM3614-75 AA Page 15 of 16
7-AAD Viability Dye
Section 11 Toxicological Information
Toxicity Data
for Hazardous Ingredients
Dimethyl Sulfoxide Oral LD50 Rat : 14500 mg/kg Oral LD50 Mouse : 7920 mg/kg
Primary Routes The most likely routes of exposure are skin, eye contact and inhalation; material may
of Exposure be absorbed through the skin.
Potential Effects of May cause irritation of the eyes, skin, mucous membrane, and upper respiratory tract.
Acute Exposure Dimethyl Sulfoxide (DMSO) is readily absorbed through skin and may act as a carrier
for toxic material.
Potential Effects of Effects are similar to those for acute exposure.
Chronic Exposure
Symptoms of No specific symptoms identified.
Overexposure
Carcinogenicity This product does not contain a reportable concentration (? 0.1%) of any ingredient
listed as carcinogen by ACGIH, IARC, NTP, OSHA or 67/548/EEC Annex I.
Other Effects None identified.
Conditions Aggravated Individuals with skin, eye, or respiratory tract disorders may find these conditions
by Exposure aggravated by exposure to this product.
Section 12 Ecological Information
Ecotoxicity Toxic to fish and other water organisms.
Biodegradability No information available.
Mobility No information available.
Section 13 Disposal Considerations
Waste Disposal Dispose of waste product, unused product and contaminated packaging in compliance
with federal, state and local regulations. If unsure of the applicable requirements,
contact the authorities for information.
Section 14 Transport Information
Transportation of this product is not regulated under ICAO, IATA, US DOT, European ADR or Canadian TDG.
Section 15 Regulatory Information
US Federal and State Regulations
SARA 313 No ingredients listed
CERCLA RG’s, No ingredients listed
40 CFR 302.4
California Proposition 65 No ingredients listed
Massachusetts MSL No ingredients listed
New Jersey Dept. of No ingredients listed
Health RTK List
Pennsylvania RTK No ingredients listed
ANNEXIN V FITC / 7-AAD KIT 150 Test Doc. ID: IM3614-75 AA Page 16 of 16
7-AAD Viability Dye
Section 15 Regulatory Information (Continued)
US Federal and State Regulations
Florida Substance List No ingredients listed
EU Labeling Classification
Preparation not classified.
Canada
This product is exempt from WHMIS label and MSDS requirements.
PIN: Not applicable
Ingredients on Ingredient Dimethyl Sulfoxide
Disclosure List:
Ingredients with unknown Product is exempt
toxicological properties:
Section 16 Other Information
For further information, please contact your local Beckman Coulter representative.
WHILE BECKMAN COULTER, INC. BELIEVES THE INFORMATION CONTAINED HEREIN IS VALID AND ACCURATE, BECKMAN COULTER MAKES NO WARRANTY OR
REPRESENTATION AS TO ITS VALIDITY, ACCURACY, OR CURRENCY. BECKMAN COULTER SHALL NOT BE LIABLE OR OTHERWISE RESPONSIBLE IN ANY WAY FOR USE
OF EITHER THIS INFORMATION OR MATERIALS TO WHICH IT APPLIES. DISPOSAL OF HAZARDOUS MATERIALS MAY BE SUBJECT TO LOCAL LAWS OR REGULATIONS.
Printed in U.S.A.
|