Kit components
12/12/2002
Product code Description
349483 BD Cell Viabiliy Kit
Components:
91-0554 Thiazole Orange Solution (BD Cell Viability Kit)
91-0555 Propidium Iodide Solution (BD Cell Viability Kit)
Page 1/6
Material Safety Data Sheet
acc. to ISO/DIS 11014
Printing date 10/03/2002 Reviewed on 10/03/2002
1 Identification of substance:
路 Product details:
路 Product name: Thiazole Orange Solution
(BD Cell Viability Kit)
路 Catalog number: 349480, 349483, 91-0554, 91-0554-MSDS.0
路 Manufacturer/Supplier:
BD Biosciences
2350 Qume Drive
San Jose, CA 95131
Tel.: (877)232-8995
路 Information department: Technical Services
路 Emergency information:
In case of a chemical emergency, spill, fire, or exposure, call BD Biosciences at (877)232-8995, or
ChemTrec at (800)424-9300.
2 Composition/Data on components:
路 Chemical characterization
路 Description: Mixture consisting of the following components.
路 Dangerous components:
67-68-5 dimethyl sulfoxide 99.998%
3 Hazards identification
路 Hazard description:
Xn Harmful
路 Information pertaining to particular dangers for man and environment
Dimethylsulfoxide freely penetrates the skin and may carry dissolved chemicals into the body.
R 20/21/22 Harmful by inhalation, in contact with skin and if swallowed.
R 40 Limited evidence of a carcinogenic effect.
路 Classification system
The classification was made according to the latest editions of international substances lists, and
expanded upon from company and literature data.
路 NFPA ratings (scale 0-4)
Health = 2
1 Fire = 1
2 0 Reactivity = 0
4 First aid measures
路 General information
Symptoms of poisoning may even occur after several hours; therefore provide medical observation for at
least 48 hours after the accident.
(Contd. on page 2)
USA
DR
Page 2/6
Material Safety Data Sheet
acc. to ISO/DIS 11014
Printing date 10/03/2002 Reviewed on 10/03/2002
Product name: Thiazole Orange Solution
(BD Cell Viability Kit)
(Contd. of page 1)
路 After inhalation
Supply fresh air. If required, provide artificial respiration. Keep patient warm. Seek medical advice.
In case of unconsciousness place patient on side position for transportation.
路 After skin contact Immediately wash with water and soap and rinse thoroughly.
路 After eye contact Rinse opened eye for 15 minutes under running water. Then consult a doctor.
路 After swallowing If symptoms persist consult doctor.
路 Information for doctor Show this label.
5 Fire fighting measures
路 Suitable extinguishing agents
CO2, extinguishing powder or water spray. Fight larger fires with water spray or alcohol resistant foam.
路 Protective equipment: No special measures required.
6 Accidental release measures
路 Person-related safety precautions: Not required.
路 Measures for environmental protection: Wipe up with damp sponge or mop.
路 Measures for cleaning/collecting:
Absorb with liquid-binding material (sand, diatomite, acid binders, universal binders).
Dispose contaminated material as waste according to item 13.
Ensure adequate ventilation.
路 Additional information:
See Section 7 for information on safe handling.
See Section 8 for information on personal protection equipment.
See Section 13 for disposal information.
7 Handling and storage
路 Handling
路 Information for safe handling: Ensure good ventilation/exhaustion at the workplace.
路 Information about protection against explosions and fires: No special measures required.
路 Storage
路 Requirements to be met by storerooms and receptacles: No special requirements.
路 Information about storage in one common storage facility: Store away from oxidizing agents.
路 Further information about storage conditions: Store in cool, dry conditions in well sealed containers.
8 Exposure controls and personal protection
路 Additional information about design of technical systems: No further data; see item 7.
路 Components with limit values that require monitoring at the workplace:
The product does not contain any relevant quantities of materials with critical values that have to be
monitored at the workplace.
路 Additional information: The lists that were valid during the creation were used as basis.
(Contd. on page 3)
USA
DR
Page 3/6
Material Safety Data Sheet
acc. to ISO/DIS 11014
Printing date 10/03/2002 Reviewed on 10/03/2002
Product name: Thiazole Orange Solution
(BD Cell Viability Kit)
(Contd. of page 2)
路 Personal Protective Equipment
路 General protective and hygienic measures
Keep away from foodstuffs, beverages and feed.
Wash hands before breaks and at the end of work.
Avoid contact with the eyes and skin.
路 Breathing equipment: Not required.
路 Protection of hands:
Chemical resistant gloves.
路 Eye protection: Safety glasses
路 Body protection: Protective work clothing (lab coat).
9 Physical and chemical properties:
路 General Information
Liquid
Form:
Light Red
Color:
Odorless
Odor:
路 Change in condition
Melting point/Melting range: 18.45掳C (65掳F)
Boiling point/Boiling range: 189掳C (372掳F)
95掳C (203掳F)
路 Flash point:
270掳C (518掳F)
路 Ignition temperature:
Product is not self igniting.
路 Auto igniting:
Product does not present an explosion hazard.
路 Danger of explosion:
路 Explosion limits:
1.8 Vol %
Lower:
Zers. Vol %
Upper:
路 Vapor pressure at 20掳C (68掳F): 2.5 hPa (2 mm Hg)
Not applicable
1.1 g/cm鲁
路 Density at 20掳C (68掳F):
路 Solubility in / Miscibility with
Fully Miscible
Water:
Not applicable
路 pH-value:
路 Solvent content:
99.9 %
Organic solvents:
0.1 %
路 Solids content:
USA
(Contd. on page 4)
DR
Page 4/6
Material Safety Data Sheet
acc. to ISO/DIS 11014
Printing date 10/03/2002 Reviewed on 10/03/2002
Product name: Thiazole Orange Solution
(BD Cell Viability Kit)
(Contd. of page 3)
10 Stability and reactivity
路 Thermal decomposition / conditions to be avoided:
No decomposition if used according to specifications.
路 Materials to be avoided: Incompatible material: strong oxidizers.
路 Dangerous reactions No dangerous reactions known
路 Dangerous products of decomposition: No dangerous decomposition products known
11 Toxicological information
路 Acute toxicity:
路 LD/LC50 values that are relevant for classification:
67-68-5 dimethyl sulfoxide
Oral LD50 14500 mg/kg (rat)
路 Primary irritant effect:
路 on the skin: No irritant effect.
路 on the eye: No irritating effect.
路 Sensitization: No sensitizing effects known.
路 Additional toxicological information:
The product shows the following dangers according to internally approved calculation methods for
preparations:
Harmful
Possible Mutagen
Possible Carcinogen
When used and handled according to specifications, the product does not have any harmful effects
according to our experience and the information provided to us.
12 Ecological information:
路 Ecotoxical effects:
路 Other information:
The ecological effects have not been thoroughly investigated, but currently none have been identified.
路 General notes: Water hazard class 1 (Self-assessment): slightly hazardous for water.
13 Disposal considerations
路 Product:
路 Recommendation
Must adhere to state and federal regulations.
Must not be disposed of with solid waste.
Disposal must be made according to the regulations found in 40 CFR 261.
This product is not considered a RCRA hazardous waste.
路 Uncleaned packagings:
路 Recommendation: Disposal must be made according to state and federal regulations.
路 Recommended cleansing agent: Water, if necessary with cleansing agents.
USA
(Contd. on page 5)
DR
Page 5/6
Material Safety Data Sheet
acc. to ISO/DIS 11014
Printing date 10/03/2002 Reviewed on 10/03/2002
Product name: Thiazole Orange Solution
(BD Cell Viability Kit)
(Contd. of page 4)
14 Transport information
路 DOT regulations:
-
路 Hazard class:
路 Land transport ADR/RID (cross-border)
路 ADR/RID class: -
路 Maritime transport IMDG:
-
路 IMDG Class:
路 Marine pollutant: No
路 Air transport ICAO-TI and IATA-DGR:
路 ICAO/IATA Class: -
15 Regulations
路 TSCA (Toxic Substances Control Act)
67-68-5 dimethyl sulfoxide
路 California Proposition 65
Mercury and mercury compounds are chemicals known to the State of
California to cause reproductive toxicity. Note: Contains less than 鈮? 0.02% of an organic mercury
compound. Foreseeable use of this product does not pose a known threat reproductive toxicity.
California Proposition 65 - Warning: This product contains a chemical known to the State of California
to cause birth defects or other reproductive harm.
路 Cancerogenity categories
路 IARC (International Agency for Research on Cancer)
None of the ingredients is listed.
路 NTP (National Toxicology Program)
None of the ingredients is listed.
路 TLV (Threshold Limit Value established by ACGIH)
None of the ingredients is listed.
路 MAK (German Maximum Workplace Concentration)
None of the ingredients is listed.
路 Product related hazard informations:
The product has been classified and marked in accordance with regulations on hazardous materials.
路 Hazard symbols:
Xn Harmful
路 Hazard-determining components of labelling:
dimethyl sulfoxide
thiazole orange
路 Risk phrases:
Dimethylsulfoxide freely penetrates the skin and may carry dissolved chemicals into the body.
20/21/22 Harmful by inhalation, in contact with skin and if swallowed.
40 Limited evidence of a carcinogenic effect.
路 Safety phrases:
9 Keep container in a well-ventilated place.
25 Avoid contact with eyes.
(Contd. on page 6)
DR
Page 6/6
Material Safety Data Sheet
acc. to ISO/DIS 11014
Printing date 10/03/2002 Reviewed on 10/03/2002
Product name: Thiazole Orange Solution
(BD Cell Viability Kit)
(Contd. of page 5)
26 In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
28 After contact with skin, wash immediately with plenty of water
36/37 Wear suitable protective clothing and gloves.
60 This material and its container must be disposed of as hazardous waste.
路 National regulations
路 Technical instructions (air):
Class Share in %
NK 99.9
路 Water hazard class: Water hazard class 1 (Self-assessment): slightly hazardous for water.
16 Other information:
To the best of our knowledge, the information contained herein is accurate. However, neither Becton,
Dickinson and Company, nor any of its subsidiaries assumes any liabilities whatsoever for the accuracy
or completeness of the information contained herein. Final determination of suitability of any material is
the sole responsibility of the user. All materials may present unknown hazards and should be used with
caution. Although certain hazards are described herein, we cannot guarantee that these are the only
hazards that exist.
路 Department issuing MSDS: Safety & Environment Department
路 Contact: Technical Service Representative
USA
DR
Page 1/5
Material Safety Data Sheet
acc. to ISO/DIS 11014
Printing date 10/03/2002 Reviewed on 10/03/2002
1 Identification of substance:
路 Product details:
路 Product name: Propidium Iodide Solution
(BD Cell Viability Kit)
路 Catalog number: 349480, 349483, 91-0555, 91-0555-MSDS.0
路 Manufacturer/Supplier:
BD Biosciences
2350 Qume Drive
San Jose, CA 95131
Tel.: (877)232-8995
路 Information department: Technical Services
路 Emergency information:
In case of a chemical emergency, spill, fire, or exposure, call BD Biosciences at (877)232-8995, or
ChemTrec at (800)424-9300.
2 Composition/Data on components:
路 Chemical characterization
路 Description: Mixture consisting of the following components.
路 Dangerous components:
25535-16-4 propidium iodide 0.29%
3 Hazards identification
路 Hazard description:
This product contains no hazardous constituents, or the concentration of all chemical constituents are
below the regulatory threshold limits described by Occupational Safety Health Administration Hazard
Communication Standard 29 CFR 1910.1200 and the European Directive 91/155/EEC, and 93/112/EC.
路 Classification system
The classification was made according to the latest editions of international substances lists, and
expanded upon from company and literature data.
路 NFPA ratings (scale 0-4)
Health = 0
0 Fire = 0
0 0 Reactivity = 0
4 First aid measures
路 General information No special measures required.
路 After inhalation Seek medical treatment in case of complaints.
路 After skin contact Immediately wash with water and soap and rinse thoroughly.
路 After eye contact
Rinse opened eye for several minutes under running water. If symptoms persist, consult a doctor.
路 After swallowing If symptoms persist consult doctor.
(Contd. on page 2)
USA
DR
Page 2/5
Material Safety Data Sheet
acc. to ISO/DIS 11014
Printing date 10/03/2002 Reviewed on 10/03/2002
Product name: Propidium Iodide Solution
(BD Cell Viability Kit)
(Contd. of page 1)
路 Information for doctor Show this label.
5 Fire fighting measures
路 Suitable extinguishing agents
CO2, extinguishing powder or water spray. Fight larger fires with water spray or alcohol resistant foam.
路 Protective equipment: No special measures required.
6 Accidental release measures
路 Person-related safety precautions: Not required.
路 Measures for environmental protection: Wipe up with damp sponge or mop.
路 Measures for cleaning/collecting: No special measures required.
路 Additional information: No dangerous substances are released.
7 Handling and storage
路 Handling
路 Information for safe handling: No special measures required.
路 Information about protection against explosions and fires: No special measures required.
路 Storage
路 Requirements to be met by storerooms and receptacles: No special requirements.
路 Information about storage in one common storage facility: Store away from oxidizing agents.
路 Further information about storage conditions: Store in cool, dry conditions in well sealed containers.
路 Storage class
路 Class according to regulation on flammable liquids: Void
8 Exposure controls and personal protection
路 Additional information about design of technical systems: No further data; see item 7.
路 Components with limit values that require monitoring at the workplace:
The product does not contain any relevant quantities of materials with critical values that have to be
monitored at the workplace.
路 Additional information: The lists that were valid during the creation were used as basis.
路 Personal Protective Equipment
路 General protective and hygienic measures
The usual precautionary measures for handling chemicals should be followed.
路 Breathing equipment: Not required.
路 Protection of hands:
Chemical resistant gloves.
路 Eye protection: Safety glasses
(Contd. on page 3)
USA
DR
Page 3/5
Material Safety Data Sheet
acc. to ISO/DIS 11014
Printing date 10/03/2002 Reviewed on 10/03/2002
Product name: Propidium Iodide Solution
(BD Cell Viability Kit)
(Contd. of page 2)
路 Body protection: Protective work clothing (lab coat).
9 Physical and chemical properties:
路 General Information
Liquid
Form:
Light red
Color:
Odorless
Odor:
Undetermined
路 Change in condition
0掳C (32掳F)
路 Melting point/Melting range:
100掳C (212掳F)
路 Boiling point/Boiling range:
Not applicable
路 Flash point:
Product is not self igniting.
路 Auto igniting:
Product does not present an explosion hazard.
路 Danger of explosion:
路 Vapor pressure at 20掳C (68掳F): 23 hPa (17 mm Hg)
Not applicable
1 g/cm鲁
路 Density at 20掳C (68掳F):
路 Solubility in / Miscibility with
Soluble
Water:
Not applicable
路 pH-value:
路 Solvent content:
0.0 %
Organic solvents:
99.7 %
Water:
0.3 %
路 Solids content:
10 Stability and reactivity
路 Thermal decomposition / conditions to be avoided:
No decomposition if used according to specifications.
路 Materials to be avoided: Incompatible material: strong oxidizers.
路 Dangerous reactions No dangerous reactions known
路 Dangerous products of decomposition: No dangerous decomposition products known
11 Toxicological information
路 Acute toxicity:
路 Primary irritant effect:
路 on the skin: No irritant effect.
路 on the eye: No irritating effect.
路 Sensitization: No sensitizing effects known.
(Contd. on page 4)
USA
DR
Page 4/5
Material Safety Data Sheet
acc. to ISO/DIS 11014
Printing date 10/03/2002 Reviewed on 10/03/2002
Product name: Propidium Iodide Solution
(BD Cell Viability Kit)
(Contd. of page 3)
路 Additional toxicological information:
The product is not subject to OSHA classification according to internally approved calculation methods
for preparations.
Possible Mutagen
Possible Carcinogen
When used and handled according to specifications, the product does not have any harmful effects
according to our experience and the information provided to us.
12 Ecological information:
路 Ecotoxical effects:
路 Other information:
The ecological effects have not been thoroughly investigated, but currently none have been identified.
路 General notes: Generally not hazardous for water.
13 Disposal considerations
路 Product:
路 Recommendation
Must adhere to state and federal regulations.
Smaller quantities can be disposed of with solid waste.
This product is not considered a RCRA hazardous waste.
Disposal must be made according to the regulations found in 40 CFR 261.
路 Uncleaned packagings:
路 Recommendation: Disposal must be made according to state and federal regulations.
路 Recommended cleansing agent: Water, if necessary with cleansing agents.
14 Transport information
路 DOT regulations:
-
路 Hazard class:
路 Land transport ADR/RID (cross-border)
路 ADR/RID class: -
路 Maritime transport IMDG:
-
路 IMDG Class:
路 Marine pollutant: No
路 Air transport ICAO-TI and IATA-DGR:
路 ICAO/IATA Class: -
15 Regulations
路 TSCA (Toxic Substances Control Act)
None of the ingredients is listed.
(Contd. on page 5)
USA
DR
Page 5/5
Material Safety Data Sheet
acc. to ISO/DIS 11014
Printing date 10/03/2002 Reviewed on 10/03/2002
Product name: Propidium Iodide Solution
(BD Cell Viability Kit)
(Contd. of page 4)
路 Cancerogenity categories
路 IARC (International Agency for Research on Cancer)
None of the ingredients is listed.
路 NTP (National Toxicology Program)
None of the ingredients is listed.
路 TLV (Threshold Limit Value established by ACGIH)
None of the ingredients is listed.
路 MAK (German Maximum Workplace Concentration)
None of the ingredients is listed.
路 Product related hazard informations:
Observe the general safety regulations when handling chemicals
The product is not subject to identification regulations pertaining to regulations on hazardous materials.
路 National regulations
路 Additional classification according to Decree on Hazardous Materials:
CERCLA: This product does not contain chemicals having a Reportable Quantity (RQ).
SARA 311/312: May be subject to reporting.
313: Not subject to reporting.
路 Classification according to VbF: Void
路 Water hazard class: Generally not hazardous for water.
16 Other information:
To the best of our knowledge, the information contained herein is accurate. However, neither Becton,
Dickinson and Company, nor any of its subsidiaries assumes any liabilities whatsoever for the accuracy
or completeness of the information contained herein. Final determination of suitability of any material is
the sole responsibility of the user. All materials may present unknown hazards and should be used with
caution. Although certain hazards are described herein, we cannot guarantee that these are the only
hazards that exist.
路 Department issuing MSDS: Safety & Environment Department
路 Contact: Technical Service Representative
USA
DR
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