Immunochemistry Technologies, LLC
MATERIAL SAFETY DATA SHEET
www.immunochemistry.com
9401 James Avenue South, Suite 155 Document: F11-01-115-02-A
Bloomington, MN 55431-2500 Effective Date: 12/31/02
phone 1-800-829-3194, 952-888-8788 Supercedes: none
fax 952-888-8988 Page 1 of 1
EMERGENCY # FOR NATIONAL RESPONSE CENTER (800) 424-8802
CHEMICAL
Product Name: Acridine Orange Stain (AOS) at
IDENTIFICATION
266 ug/mL (1mM)
Catalog #: 599, and 6130
Synonyms of Active Reagent: AO; 3, 6-bis-diethylaminoacridine; euchrysine; rhoduline orange; waxoline orange;
basic orange 14; CI 46005; sumitomo acridine orange RK
CAS # of Active Reagent: 494-38-2, 65-61-2
Chemical Name of Active Reagent: N, N, N', N'-tetramethyl-3, 6-acridinediamine
Molecular Weight of Active Reagent: 265.36
Chemical Formula of Active Reagent: C17H19N3
HAZARDS AOS contains acridine orange reagent (AOR) at 266 ug/mL (equal to 1 millimolar) in DI H2O. AOR is not currently listed as a
IDENTIFICATION carcinogen by ACGIH, IARC, NIOSH, NTP, or OSHA; the available data are insufficient to evaluate carcinogenicity. This
staining reagent should always be handled using protective gloves, clothing, and eyewear. Incompatible with strong oxidizing
agents.
CHARACTERISTICS AOS is a clear, red-orange solution.
Boiling point 癈: No data available.
Vapor pressure (mm Hg): No data available.
Vapor density: No data available.
Solubility in water: In solution.
Specific gravity: Not applicable.
% Volatile by volume: Not applicable.
Evaporation rate: No data available.
pH: 3.8 ?4.2
STORAGE AND AOS, as supplied by ICT, is stable for 24 months at 2-8篊 when stored in tightly closed amber vials and protected from light.
STABILITY Vials may be hazardous when empty since they contain AOR residues. Hygroscopic.
HEALTH HAZARD AOS contains AOR, which is harmful if swallowed, inhaled, or absorbed through the skin. AOR has shown mutagenic effects in
laboratory experiments. Toxicity of AO has not been thoroughly investigated.
SAFETY CONTROL Personal protective equipment, such as lab coat, safety glasses, gloves should be worn at all times while handling this product.
MEASURES Maintain eye wash fountain and safety shower in work area. Safe laboratory practices should be followed. Always wash
thoroughly after handling. Avoid prolonged exposure. Never take internally. Do not breathe vapor or mist.
EXPOSURE No exposure guidelines are available.
GUIDELINES
FIRST AID AOS may cause irritation; avoid any contact with skin, eyes, or take internally. Do not breathe vapor or mist. Carefully remove
MEASURES and dispose of gloves after use. Remove contaminated clothing and shoes; wash before reuse.
Skin: Wash thoroughly with plenty of soap and water for at least 15 minutes. Remove contaminated clothing and
shoes. Launder contaminated clothing. Throw away contaminated shoes.
Eyes: Immediately flush with plenty of water for at least 15 minutes.
Ingestion: If solution is swallowed, induce vomiting and/or drink water or milk and seek medical attention. Wash out
mouth with water.
Inhalation: Remove to fresh air, seek medical advice.
FIRE/EXPLOSION The 266 ug/ml solution of acridine orange stain is not a potential fire or explosion hazard. However, AOR decomposes when
HAZARD burning and produces toxic gasses: hydrogen chloride; nitrogen oxides; carbon monoxide; and carbon dioxide. Hazardous
polymerization has not been reported.
ACCIDENTAL For simple counter top spills, use protective gloves and clothing when promptly cleaning up spills. Spilled solutions may be
RELEASE adsorbed into paper toweling and disposed of according to local waste management ordinances. For extremely large spills,
MEASURES ventilate area and evacuate all unnecessary employees. Wear appropriate personal protective equipment including: neoprene
(or equivalent) gloves, safety glasses with side shields, and lab coat. Contain the spill and dispose of in compliance with
federal, state, and local regulations. US regulations (CERCLA) require reporting spills and releases to soil, water, and air in
excess of reportable quantities. Toll free number to US Coast Guard national response center is (800) 424-8802.
THE ABOVE INFORMATION IS BELIEVED TO BE CORRECT BUT IS NOT NECESSARILY ALL-INCLUSIVE AND SHOULD BE USED
ONLY AS A GUIDE. IMMUNOCHEMISTRY TECHNOLOGIES, LLC SHALL NOT BE HELD LIABLE FOR ANY DAMAGE RESULTING FROM
HANDLING OR FROM CONTACT WITH THE ABOVE PRODUCT. INDIVIDUALS RECEIVING THE INFORMATION MUST EXERCISE THEIR
INDEPENDENT JUDGMENT IN DETERMINING ITS APPROPRIATENESS FOR A PARTICULAR PURPOSE.
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