Material Safety Data Sheet
Section 1: Product Information
Product Name: NWLSSTM Nitrotyrosine ELISA
Product Number: NWK-NTR01
Manufacturer's Information:
Company Name: Northwest Life Science Specialties, LLC
Address: 5131 NE 94th Avenue
Vancouver, WA 98683
Emergency Phone: 888-449-3091, 360-449-3091
Section 2: Components
Part Description Quantity
40X Concentrated Wash Buffer containing Tween-20: 20 mL
10X Concentrated Dilution Buffer. Protein stabilized 10 mL
phosphate buffered saline containing 2-chloroacetamide
preservative:
TMB Substrate: 22 mL
Stop Solution containing citric acid: 22 mL
Lyophilized Nitrotyrosine Standard. 1 mL
Concentration is lot specific and indicated on label. (reconstituted volume)
Lyophilized Biotinylated Anti-nitrotyrosine in protein 1 mL
stabilized buffer with 2-chloroacetamide preservative. (reconstituted volume)
Lyophilized concentrated Streptavidin-peroxidase 1 mL
conjugate with 2-chloroacetamide preservative. (reconstituted volume)
96 well microplates (12 X 8 well strips) pre-coated with 2 each
anti-nitrotyrosine antibody:
Section 3: Hazardous Ingredients
Known Hazardous Components: CAS Number Percent
2-chloroacetamide 79-07-2 0.5
Citric acid 77-92-9 41.0
Section 4: Fire and Explosion Data
None of the components of this product pose a significant risk in case of fire. Fire
fighting media should be selected as appropriate to surrounding fire conditions.
Firefighters should wear suitable protective gear to limit exposure possibilities including
inhalation.
Section 5: Stability and Reactivity
The unopened product is stable until the date indicated on the label if stored as specified.
Individual and reconstituted components are stable as specified in the product insert.
There are no known hazardous reactions between these components.
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Section 6: Toxicological Information
2-chloroacetamide: Test Result Route Species
LD50 122 mg/kg Oral Rabbit
LD50 31 mg/kg Oral Dog
Acute Effects:
Toxic if swallowed.
May cause sensitization by skin contact.
Moderately irritating to the skin.
Moderately irritating to the eyes.
Chronic Adverse Effects:
May include: sensitization burning in throat coughing wheezing - breathing
difficulties shortness of breath/breathing difficulty headache
nausea/vomiting. Repeated exposure to a highly toxic material may produce
general deterioration of health by an accumulation in one or many human
organs
Target Organs:
May cause damage to the following organs: lungs, digestive system, upper
respiratory tract, head.
Test Result Route Species
Citric Acid
LD50 >5000 mg/kg Oral Rat
Acute Affects:
No specific hazard if swallowed.
Moderately irritating to the skin.
Moderately irritating to the eyes.
Adverse Effects:
None noted
Section 7: Preventive Measures / Exposure Control
The physical, chemical and toxicological properties of these components have not been
fully investigated. It is recommended that laboratory personnel minimize direct skin or
eye contact by following standard laboratory safety procedures when handling this
product. Standard safety procedures should include wearing OSHA approved safety
glasses, gloves and protective clothing.
Section 8: First Aid Measures
Skin Exposure Remove any contaminated clothing. Flush affected area with copious
amounts of water for at least 15 minutes then wash thoroughly. If
irritation persists, contact a physician to seek medical advice.
Eye Exposure If wearing contact lenses, remove them. Flush with copious amounts
of water for at least 15 minutes.
Ingestion If swallowed and individual is conscious, wash out mouth with water.
Contact a physician to seek medical advice.
Inhalation Move to fresh air. If difficulty breathing, oxygen may be administered.
If breathing stops, administer artificial respiration and seek emergency
medical assistance.
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Section 9: Accidental Release / Spillage Measures
Ventilate area. Shut off all sources of ignition. Wear appropriate protective gear. Wipe or
pick-up spilled material, place in sealed container if necessary, dispose of in accordance
with federal, state, and local regulations. Wash spill site thoroughly after material pick-up
is complete.
Section 10: Handling and Storage
This product should be stored as recommended on the product label. All components
should be kept in tightly closed containers. Refer to the Storage section of the product
insert for additional information. This product should only be handled and used by
qualified, trained professional laboratory personnel.
Section 11: Other Information
This information is believed to be correct however does not claim to be all inclusive and
is intended as a general guide only. Northwest Life Science Specialties, LLC shall not be
held liable for any damage resulting from handling of or contact with this product or
components therein. The burden of safe use of this and other products used in connection
with this product rests solely with the user.
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