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MSDS Material Safety Data Sheet
CAS

75-60-5 7791-13-1

File Name: 75-60-5_7791-13.asp

06/24/2008                                           Kit ingredients
Product: DeadEndTM Colorimetric TUNEL System, 50 rxns
Product code: G7360
Substance number Description Count Symbols
G327 Equilibration Buffer 3 -
M828 Terminal deoxynucleotidy tranferase, 3 -
recombinant - Low Concentrate
G714 Streptavidin HRP 3 -
G715 Biotin Nucleotide Mix 3 -
V302 Proteinase K 3 Xi
G329 SSC, 20X 1 -
G716 DAB 20X Chromogen 1 -
G717 DAB 20X Buffer 1 -
G718 Hydrogen Peroxide 20X 1 -
Page 1/5
Safety Data Sheet
according to 1907/2006/EC, Article 31
Printing date 24.06.2008 Revision: 24.06.2008

* 1 Identification of substance
Trade name: Equilibration Buffer
Article number: G327
Application of the substance / the preparation: Laboratory chemicals
Manufacturer/Supplier:
Promega Corporation
2800 Woods Hollow Road
Madison, WI 53711
U.S.A.
1-608-274-4330
1-800-356-9526

MSDS author: Regulatory.Affairs@promega.com
Further information obtainable from:
Promega UK Ltd.
Delta House
Southampton Science Park
Southampton SO16 7NS
UNITED KINGDOM
Tel: (44) 23 8076 0225
Fax: (44) 23 8076 7014
FREEPHONE: 0800 378994
E-mail Address: ukcustserve@promega.com
Web Address: www.promega.com/uk/
Information in case of emergency:
CHEMTREC 1-800-424-9300
For call originating outside the United States dial 001-703-527-3887


* 2 Hazards identification
Hazard description: not applicable
Information concerning to particular hazards to man and environment
The product does not have to be labelled due to the calculation procedure of the "General Classification
guideline for preparations of the EU" in the latest valid version.
Classification system:
The classification is in line with current EC lists. It is extended, by information from technical literature and
company information.


* 3 Composition/information on ingredients
Chemical characterization
Description: Mixture of substances listed below with nonhazardous additions.
(Contd. on page 2)
GB




DR
Page 2/5
Safety Data Sheet
according to 1907/2006/EC, Article 31
Printing date 24.06.2008 Revision: 24.06.2008

Trade name: Equilibration Buffer

(Contd. of page 1)
Components:
CAS: 75-60-5 dimethylarsinic acid T; R 23/25 1.0-5.0%
EINECS: 200-883-4
Additional information: For the wording of the listed risk phrases refer to section 16.


* 4 First aid measures
General information: No special measures required.
After inhalation: Supply fresh air; consult doctor in case of complaints.
After skin contact: Generally the product does not irritate the skin.
After eye contact: Rinse opened eye for several minutes under running water.
After swallowing: If symptoms persist consult doctor.


* 5 Fire fighting measures
Suitable extinguishing agents:
CO2, powder or water spray. Fight larger fires with water spray or alcohol resistant foam.
Protective equipment: No special measures required.


* 6 Accidental release measures
Person-related safety precautions: Not required.
Measures for environmental protection: Do not allow to enter sewers/ surface or ground water.
Measures for cleaning/collecting:
Absorb with liquid-binding material (sand, diatomite, acid binders, universal binders, sawdust).
Additional information: No dangerous substances are released.


* 7 Handling and storage
Handling
Information for safe handling: No special measures required.
Information about fire - and explosion protection: No special measures required.
Storage
Requirements to be met by storerooms and receptacles: No special requirements.
Information about storage in one common storage facility: Not required.
Further information about storage conditions: None.


* 8 Exposure controls and personal protection gear
Additional information about design of technical facilities: No further data; see item 7.
Ingredients with limit values that require monitoring at the workplace:
The product does not contain any relevant quantities of materials with critical values that have to be monitored at
the workplace.
Additional information: The lists valid during the making were used as basis.
Personal protective equipment
General protective and hygienic measures
The usual precautionary measures are to be adhered to when handling chemicals.
(Contd. on page 3)
GB




DR
Page 3/5
Safety Data Sheet
according to 1907/2006/EC, Article 31
Printing date 24.06.2008 Revision: 24.06.2008

Trade name: Equilibration Buffer

(Contd. of page 2)
Respiratory protection: Not required.
Protection of hands:
Protective gloves.
Selection of the glove material on consideration of the penetration times, rates of diffusion and the degradation
Material of gloves:
The selection of the suitable gloves does not only depend on the material, but also on further marks of quality and
varies from manufacturer to manufacturer. As the product is a preparation of several substances, the resistance
of the glove material can not be calculated in advance and has therefore to be checked prior to the application.
Penetration time of glove material:
The exact break trough time has to be found out by the manufacturer of the protective gloves and has to be
observed.
Eye protection: Goggles recommended during refilling.


* 9 Physical and chemical properties
General Information
Fluid
Form:
Colourless
Colour:
Characteristic
Odour:
Change in condition
Melting point/Melting range: 0癈
Boiling point/Boiling range: 100癈
Not applicable
Flash point:
Product is not selfigniting.
Self-igniting:
Product does not present an explosion hazard.
Danger of explosion:
1 g/cm?br> Density at 20癈:
Solubility in / Miscibility with
Not miscible or difficult to mix
Water:
6.8
pH-value at 20癈:
Solvent content:
0.0 %
Organic solvents:
96.8 %
Water:
3.2 %
Solids content:



10 Stability and reactivity
Thermal decomposition / conditions to be avoided: No decomposition if used according to specifications.
Dangerous reactions: Reacts with strong acids
Dangerous decomposition products:
Arsenic compounds
Carbon monoxide and carbon dioxide
GB
(Contd. on page 4)




DR
Page 4/5
Safety Data Sheet
according to 1907/2006/EC, Article 31
Printing date 24.06.2008 Revision: 24.06.2008

Trade name: Equilibration Buffer

(Contd. of page 3)


* 11 Toxicological information
Acute toxicity:
LD/LC50 values relevant for classification:
7791-13-1 Cobalt Chloride Hexahydrate
Oral LD50 766 mg/kg (Rat)
Primary irritant effect:
on the skin: No irritant effect.
on the eye: Irritating effect.
Sensitization: No sensitizing effects known.
Additional toxicological information:
The product is not subject to classification according to the calculation method of the General EU Classification
Guidelines for Preparations as issued in the latest version.
When used and handled according to specifications, the product does not have any harmful effects to our
experience and the information provided to us.


12 Ecological information
General notes:
Water hazard class 2 (German Regulation) (Self-assessment): hazardous for water.
Do not allow product to reach ground water, water course or sewage system.
Danger to drinking water if even small quantities leak into the ground.


* 13 Disposal considerations
Product:
Recommendation
Smaller quantities can be disposed of with household waste.
Must not be disposed together with household garbage. Do not allow product to reach sewage system.
Uncleaned packaging:
Recommendation: Disposal must be made according to official regulations.


* 14 Transport information
Contact Promega Safety Department for additional transport information.
Land transport ADR/RID (cross-border)
ADR/RID class: -
Not regulated
Maritime transport IMDG:
-
IMDG Class:
Marine pollutant: No
Air transport ICAO-TI and IATA-DGR:
ICAO/IATA Class: -
GB
(Contd. on page 5)




DR
Page 5/5
Safety Data Sheet
according to 1907/2006/EC, Article 31
Printing date 24.06.2008 Revision: 24.06.2008

Trade name: Equilibration Buffer

(Contd. of page 4)


* 15 Regulations
Labelling according to EU guidelines:
Observe the general safety regulations when handling chemicals
The product is not subject to identification regulations under EU Directives and the Ordinance on Hazardous
Materials (German GefStoffV).
Special labelling of certain preparations:
Safety data sheet available for professional user on request.
National regulations
Waterhazard class: Water hazard class 2 (Self-assessment): hazardous for water.


16 Other information
This information is based on our present knowledge. However, this shall not constitute a guarantee for any
specific product features and shall not establish a legally valid contractual relationship.
Relevant R-phrases
23/25 Toxic by inhalation and if swallowed.
Department issuing MSDS:
Promega Corporation
Safety Department
2800 Woods Hollow Road
Madison, WI 53711
U.S.A.
Contact:
Promega UK
Delta House, Enterprise Road
Chilworth Science Park
Southampton SO16 7NS
UNITED KINGDOM
Free Phone +44 800 559900
Free Fax +44 800 181037
* Data compared to the previous version altered.
GB




DR
Page 1/5
Safety Data Sheet
according to 1907/2006/EC, Article 31
Printing date 24.06.2008 Revision: 24.06.2008

* 1 Identification of substance
Trade name: Terminal deoxynucleotidy tranferase,
Article number: M828
Application of the substance / the preparation: Laboratory chemicals
Manufacturer/Supplier:
Promega Corporation
2800 Woods Hollow Road
Madison, WI 53711
U.S.A.
1-608-274-4330
1-800-356-9526

MSDS author: Regulatory.Affairs@promega.com
Further information obtainable from:
Promega UK Ltd.
Delta House
Southampton Science Park
Southampton SO16 7NS
UNITED KINGDOM
Tel: (44) 23 8076 0225
Fax: (44) 23 8076 7014
FREEPHONE: 0800 378994
E-mail Address: ukcustserve@promega.com
Web Address: www.promega.com/uk/
Information in case of emergency:
CHEMTREC 1-800-424-9300
For call originating outside the United States dial 001-703-527-3887


2 Hazards identification
Hazard description: not applicable
Information concerning to particular hazards to man and environment
The product does not have to be labelled due to the calculation procedure of the "General Classification
guideline for preparations of the EU" in the latest valid version.
Classification system:
The classification is in line with current EC lists. It is extended, by information from technical literature and
company information.


3 Composition/information on ingredients
Chemical characterization
Description: Mixture of substances listed below with nonhazardous additions.
(Contd. on page 2)
GB




DR
Page 2/5
Safety Data Sheet
according to 1907/2006/EC, Article 31
Printing date 24.06.2008 Revision: 24.06.2008

Trade name: Terminal deoxynucleotidy tranferase,

(Contd. of page 1)
Components:
CAS: 56-81-5 glycerol 25-50%
EINECS: 200-289-5
Additional information: For the wording of the listed risk phrases refer to section 16.


4 First aid measures
General information: No special measures required.
After inhalation: Supply fresh air; consult doctor in case of complaints.
After skin contact: Generally the product does not irritate the skin.
After eye contact: Rinse opened eye for several minutes under running water.
After swallowing: If symptoms persist consult doctor.


5 Fire fighting measures
Suitable extinguishing agents: Use fire extinguishing methods suitable to surrounding conditions.
Protective equipment: No special measures required.


6 Accidental release measures
Person-related safety precautions: Not required.
Measures for environmental protection:
Dilute with plenty of water.
Do not allow to enter sewers/ surface or ground water.
Measures for cleaning/collecting:
Absorb with liquid-binding material (sand, diatomite, acid binders, universal binders, sawdust).
Additional information: No dangerous substances are released.


7 Handling and storage
Handling
Information for safe handling: No special measures required.
Information about fire - and explosion protection: No special measures required.
Storage
Requirements to be met by storerooms and receptacles: No special requirements.
Information about storage in one common storage facility: Not required.
Further information about storage conditions: None.


8 Exposure controls and personal protection gear
Additional information about design of technical facilities: No further data; see item 7.
Ingredients with limit values that require monitoring at the workplace:
56-81-5 glycerol
WEL Long-term value: 10 mg/m?br> Additional information: The lists valid during the making were used as basis.
(Contd. on page 3)
GB




DR
Page 3/5
Safety Data Sheet
according to 1907/2006/EC, Article 31
Printing date 24.06.2008 Revision: 24.06.2008

Trade name: Terminal deoxynucleotidy tranferase,

(Contd. of page 2)

Personal protective equipment
General protective and hygienic measures
The usual precautionary measures are to be adhered to when handling chemicals.
Respiratory protection: Not required.
Protection of hands:
Protective gloves.
Selection of the glove material on consideration of the penetration times, rates of diffusion and the degradation
Material of gloves:
The selection of the suitable gloves does not only depend on the material, but also on further marks of quality and
varies from manufacturer to manufacturer. As the product is a preparation of several substances, the resistance
of the glove material can not be calculated in advance and has therefore to be checked prior to the application.
Penetration time of glove material:
The exact break trough time has to be found out by the manufacturer of the protective gloves and has to be
observed.
Eye protection: Goggles recommended during refilling.


9 Physical and chemical properties
General Information
Fluid
Form:
Colourless
Colour:
Characteristic
Odour:
Change in condition
Melting point/Melting range: undetermined
Boiling point/Boiling range: undetermined
> 100癈
Flash point:
400癈
Ignition temperature:
Product is not selfigniting.
Self-igniting:
Product does not present an explosion hazard.
Danger of explosion:
Explosion limits:
0.9 Vol %
Lower:
0.1 hPa
Vapour pressure at 20癈:
1.145 g/cm?br> Density at 20癈:
Solubility in / Miscibility with
Fully miscible
Water:
6.4
pH-value at 20癈:
Solvent content:
50.0 %
Organic solvents:
48.5 %
Water:



10 Stability and reactivity
Thermal decomposition / conditions to be avoided: No decomposition if used according to specifications.
Dangerous reactions: No dangerous reactions known
(Contd. on page 4)
GB




DR
Page 4/5
Safety Data Sheet
according to 1907/2006/EC, Article 31
Printing date 24.06.2008 Revision: 24.06.2008

Trade name: Terminal deoxynucleotidy tranferase,

(Contd. of page 3)
Dangerous decomposition products: No dangerous decomposition products known


11 Toxicological information
Acute toxicity:
Primary irritant effect:
on the skin: No irritant effect.
on the eye: Irritating effect.
Sensitization: No sensitizing effects known.
Additional toxicological information:
The product is not subject to classification according to the calculation method of the General EU Classification
Guidelines for Preparations as issued in the latest version.
When used and handled according to specifications, the product does not have any harmful effects to our
experience and the information provided to us.


12 Ecological information
General notes:
Water hazard class 1 (German Regulation) (Self-assessment): slightly hazardous for water.
Do not allow undiluted product or large quantities of it to reach ground water, water course or sewage system.


13 Disposal considerations
Product:
Recommendation
Smaller quantities can be disposed of with household waste.
Must not be disposed together with household garbage. Do not allow product to reach sewage system.
Uncleaned packaging:
Recommendation: Disposal must be made according to official regulations.
Recommended cleansing agents: Water, if necessary together with cleansing agents.


* 14 Transport information
Contact Promega Safety Department for additional transport information.
Land transport ADR/RID (cross-border)
ADR/RID class: -
Not regulated
Maritime transport IMDG:
-
IMDG Class:
Marine pollutant: No
Air transport ICAO-TI and IATA-DGR:
ICAO/IATA Class: -
GB
(Contd. on page 5)




DR
Page 5/5
Safety Data Sheet
according to 1907/2006/EC, Article 31
Printing date 24.06.2008 Revision: 24.06.2008

Trade name: Terminal deoxynucleotidy tranferase,

(Contd. of page 4)


15 Regulations
Labelling according to EU guidelines:
Observe the general safety regulations when handling chemicals
The product is not subject to identification regulations under EU Directives and the Ordinance on Hazardous
Materials (German GefStoffV).
Special labelling of certain preparations:
Safety data sheet available for professional user on request.
National regulations
Technical instructions (air):
Class Share in %
Water 48.5
NK 50.0

Waterhazard class: Water hazard class 1 (Self-assessment): slightly hazardous for water.


16 Other information
This information is based on our present knowledge. However, this shall not constitute a guarantee for any
specific product features and shall not establish a legally valid contractual relationship.
Department issuing MSDS:
Promega Corporation
Safety Department
2800 Woods Hollow Road
Madison, WI 53711
U.S.A.
Contact:
Promega UK
Delta House, Enterprise Road
Chilworth Science Park
Southampton SO16 7NS
UNITED KINGDOM
Free Phone +44 800 559900
Free Fax +44 800 181037
* Data compared to the previous version altered.
GB




DR
Page 1/5
Safety Data Sheet
according to 1907/2006/EC, Article 31
Printing date 24.06.2008 Revision: 24.06.2008

* 1 Identification of substance
Trade name: Streptavidin HRP
Article number: G714
Application of the substance / the preparation: Laboratory chemicals
Manufacturer/Supplier:
Promega Corporation
2800 Woods Hollow Road
Madison, WI 53711
U.S.A.
1-608-274-4330
1-800-356-9526

MSDS author: Regulatory.Affairs@promega.com
Further information obtainable from:
Promega UK Ltd.
Delta House
Southampton Science Park
Southampton SO16 7NS
UNITED KINGDOM
Tel: (44) 23 8076 0225
Fax: (44) 23 8076 7014
FREEPHONE: 0800 378994
E-mail Address: ukcustserve@promega.com
Web Address: www.promega.com/uk/
Information in case of emergency:
CHEMTREC 1-800-424-9300
For call originating outside the United States dial 001-703-527-3887


2 Hazards identification
Hazard description: not applicable
Information concerning to particular hazards to man and environment
The product does not have to be labelled due to the calculation procedure of the "General Classification
guideline for preparations of the EU" in the latest valid version.
Classification system:
The classification is in line with current EC lists. It is extended, by information from technical literature and
company information.


3 Composition/information on ingredients
Chemical characterization
Description: Mixture of substances listed below with nonhazardous additions.
Components: Void
(Contd. on page 2)
GB




DR
Page 2/5
Safety Data Sheet
according to 1907/2006/EC, Article 31
Printing date 24.06.2008 Revision: 24.06.2008

Trade name: Streptavidin HRP

(Contd. of page 1)
Additional information: For the wording of the listed risk phrases refer to section 16.


4 First aid measures
General information: No special measures required.
After inhalation: Supply fresh air; consult doctor in case of complaints.
After skin contact: Generally the product does not irritate the skin.
After eye contact: Rinse opened eye for several minutes under running water.
After swallowing: If symptoms persist consult doctor.


5 Fire fighting measures
Suitable extinguishing agents:
CO2, powder or water spray. Fight larger fires with water spray or alcohol resistant foam.
Protective equipment: No special measures required.


6 Accidental release measures
Person-related safety precautions: Not required.
Measures for environmental protection: No special measures required.
Measures for cleaning/collecting:
Absorb with liquid-binding material (sand, diatomite, acid binders, universal binders, sawdust).
Additional information: No dangerous substances are released.


7 Handling and storage
Handling
Information for safe handling: No special measures required.
Information about fire - and explosion protection: No special measures required.
Storage
Requirements to be met by storerooms and receptacles: No special requirements.
Information about storage in one common storage facility: Not required.
Further information about storage conditions: None.


8 Exposure controls and personal protection gear
Additional information about design of technical facilities: No further data; see item 7.
Ingredients with limit values that require monitoring at the workplace:
The product does not contain any relevant quantities of materials with critical values that have to be monitored at
the workplace.
Additional information: The lists valid during the making were used as basis.
Personal protective equipment
General protective and hygienic measures
The usual precautionary measures are to be adhered to when handling chemicals.
Respiratory protection: Not required.
Protection of hands:
Protective gloves.
Selection of the glove material on consideration of the penetration times, rates of diffusion and the degradation
(Contd. on page 3)
GB




DR
Page 3/5
Safety Data Sheet
according to 1907/2006/EC, Article 31
Printing date 24.06.2008 Revision: 24.06.2008

Trade name: Streptavidin HRP

(Contd. of page 2)
Material of gloves:
The selection of the suitable gloves does not only depend on the material, but also on further marks of quality and
varies from manufacturer to manufacturer. As the product is a preparation of several substances, the resistance
of the glove material can not be calculated in advance and has therefore to be checked prior to the application.
Penetration time of glove material:
The exact break trough time has to be found out by the manufacturer of the protective gloves and has to be
observed.
Eye protection: Goggles recommended during refilling.


9 Physical and chemical properties
General Information
Fluid
Form:
Colourless
Colour:
Characteristic
Odour:
Change in condition
Melting point/Melting range: 0癈
Boiling point/Boiling range: 100癈
Not applicable
Flash point:
Product is not selfigniting.
Self-igniting:
Product does not present an explosion hazard.
Danger of explosion:
1 g/cm?br> Density at 20癈:
Solubility in / Miscibility with
Not miscible or difficult to mix
Water:
Solvent content:
0.0 %
Organic solvents:
99.9 %
Water:
0.1 %
Solids content:



10 Stability and reactivity
Thermal decomposition / conditions to be avoided: No decomposition if used according to specifications.
Dangerous reactions: No dangerous reactions known
Dangerous decomposition products: No dangerous decomposition products known


11 Toxicological information
Acute toxicity:
Primary irritant effect:
on the skin: No irritant effect.
on the eye: Irritating effect.
Sensitization: No sensitizing effects known.
Additional toxicological information:
The product is not subject to classification according to the calculation method of the General EU Classification
Guidelines for Preparations as issued in the latest version.
(Contd. on page 4)
GB




DR
Page 4/5
Safety Data Sheet
according to 1907/2006/EC, Article 31
Printing date 24.06.2008 Revision: 24.06.2008

Trade name: Streptavidin HRP

(Contd. of page 3)
When used and handled according to specifications, the product does not have any harmful effects to our
experience and the information provided to us.


12 Ecological information
General notes: Generally not hazardous for water.


13 Disposal considerations
Product:
Recommendation
Smaller quantities can be disposed of with household waste.
Must not be disposed together with household garbage. Do not allow product to reach sewage system.
Uncleaned packaging:
Recommendation: Disposal must be made according to official regulations.


* 14 Transport information
Contact Promega Safety Department for additional transport information.
Land transport ADR/RID (cross-border)
ADR/RID class: Not regulated
Maritime transport IMDG:
Marine pollutant: No


15 Regulations
Labelling according to EU guidelines:
Observe the general safety regulations when handling chemicals
The product is not subject to identification regulations under EU Directives and the Ordinance on Hazardous
Materials (German GefStoffV).
National regulations
Waterhazard class: Generally not hazardous for water.


16 Other information
This information is based on our present knowledge. However, this shall not constitute a guarantee for any
specific product features and shall not establish a legally valid contractual relationship.
Department issuing MSDS:
Promega Corporation
Safety Department
2800 Woods Hollow Road
Madison, WI 53711
U.S.A.
(Contd. on page 5)
GB




DR
Page 5/5
Safety Data Sheet
according to 1907/2006/EC, Article 31
Printing date 24.06.2008 Revision: 24.06.2008

Trade name: Streptavidin HRP

(Contd. of page 4)
Contact:
Promega UK
Delta House, Enterprise Road
Chilworth Science Park
Southampton SO16 7NS
UNITED KINGDOM
Free Phone +44 800 559900
Free Fax +44 800 181037
* Data compared to the previous version altered.
GB




DR
Page 1/5
Safety Data Sheet
according to 1907/2006/EC, Article 31
Printing date 24.06.2008 Revision: 24.06.2008

* 1 Identification of substance
Trade name: Biotin Nucleotide Mix
Article number: G715
Application of the substance / the preparation: Laboratory chemicals
Manufacturer/Supplier:
Promega Corporation
2800 Woods Hollow Road
Madison, WI 53711
U.S.A.
1-608-274-4330
1-800-356-9526

MSDS author: Regulatory.Affairs@promega.com
Further information obtainable from:
Promega UK Ltd.
Delta House
Southampton Science Park
Southampton SO16 7NS
UNITED KINGDOM
Tel: (44) 23 8076 0225
Fax: (44) 23 8076 7014
FREEPHONE: 0800 378994
E-mail Address: ukcustserve@promega.com
Web Address: www.promega.com/uk/
Information in case of emergency:
CHEMTREC 1-800-424-9300
For call originating outside the United States dial 001-703-527-3887


2 Hazards identification
Hazard description: not applicable
Information concerning to particular hazards to man and environment
The product does not have to be labelled due to the calculation procedure of the "General Classification
guideline for preparations of the EU" in the latest valid version.
Classification system:
The classification is in line with current EC lists. It is extended, by information from technical literature and
company information.


3 Composition/information on ingredients
Chemical characterization
Description: Mixture of substances listed below with nonhazardous additions.
Components: Void
(Contd. on page 2)
GB




DR
Page 2/5
Safety Data Sheet
according to 1907/2006/EC, Article 31
Printing date 24.06.2008 Revision: 24.06.2008

Trade name: Biotin Nucleotide Mix

(Contd. of page 1)
Additional information: For the wording of the listed risk phrases refer to section 16.


4 First aid measures
General information: No special measures required.
After inhalation: Supply fresh air; consult doctor in case of complaints.
After skin contact: Generally the product does not irritate the skin.
After eye contact: Rinse opened eye for several minutes under running water.
After swallowing: If symptoms persist consult doctor.


5 Fire fighting measures
Suitable extinguishing agents:
CO2, powder or water spray. Fight larger fires with water spray or alcohol resistant foam.
Protective equipment: No special measures required.


6 Accidental release measures
Person-related safety precautions: Not required.
Measures for environmental protection: No special measures required.
Measures for cleaning/collecting:
Absorb with liquid-binding material (sand, diatomite, acid binders, universal binders, sawdust).
Additional information: No dangerous substances are released.


7 Handling and storage
Handling
Information for safe handling: No special measures required.
Information about fire - and explosion protection: No special measures required.
Storage
Requirements to be met by storerooms and receptacles: No special requirements.
Information about storage in one common storage facility: Not required.
Further information about storage conditions: None.


8 Exposure controls and personal protection gear
Additional information about design of technical facilities: No further data; see item 7.
Ingredients with limit values that require monitoring at the workplace:
The product does not contain any relevant quantities of materials with critical values that have to be monitored at
the workplace.
Additional information: The lists valid during the making were used as basis.
Personal protective equipment
General protective and hygienic measures
The usual precautionary measures are to be adhered to when handling chemicals.
Respiratory protection: Not required.
Protection of hands:
Protective gloves.
Selection of the glove material on consideration of the penetration times, rates of diffusion and the degradation
(Contd. on page 3)
GB




DR
Page 3/5
Safety Data Sheet
according to 1907/2006/EC, Article 31
Printing date 24.06.2008 Revision: 24.06.2008

Trade name: Biotin Nucleotide Mix

(Contd. of page 2)
Material of gloves:
The selection of the suitable gloves does not only depend on the material, but also on further marks of quality and
varies from manufacturer to manufacturer. As the product is a preparation of several substances, the resistance
of the glove material can not be calculated in advance and has therefore to be checked prior to the application.
Penetration time of glove material:
The exact break trough time has to be found out by the manufacturer of the protective gloves and has to be
observed.
Eye protection: Goggles recommended during refilling.


9 Physical and chemical properties
General Information
Fluid
Form:
Colourless
Colour:
Characteristic
Odour:
Change in condition
Melting point/Melting range: 0癈
Boiling point/Boiling range: 100癈
Not applicable
Flash point:
Product is not selfigniting.
Self-igniting:
Product does not present an explosion hazard.
Danger of explosion:
1 g/cm?br> Density at 20癈:
Solubility in / Miscibility with
Not miscible or difficult to mix
Water:
7.6
pH-value at 20癈:
Solvent content:
0.0 %
Organic solvents:
99.8 %
Water:
0.2 %
Solids content:



10 Stability and reactivity
Thermal decomposition / conditions to be avoided: No decomposition if used according to specifications.
Dangerous reactions: No dangerous reactions known
Dangerous decomposition products: No dangerous decomposition products known


11 Toxicological information
Acute toxicity:
Primary irritant effect:
on the skin: No irritant effect.
on the eye: Irritating effect.
Sensitization: No sensitizing effects known.
(Contd. on page 4)
GB




DR
Page 4/5
Safety Data Sheet
according to 1907/2006/EC, Article 31
Printing date 24.06.2008 Revision: 24.06.2008

Trade name: Biotin Nucleotide Mix

(Contd. of page 3)
Additional toxicological information:
The product is not subject to classification according to the calculation method of the General EU Classification
Guidelines for Preparations as issued in the latest version.
When used and handled according to specifications, the product does not have any harmful effects to our
experience and the information provided to us.


12 Ecological information
General notes: Generally not hazardous for water.


13 Disposal considerations
Product:
Recommendation
Smaller quantities can be disposed of with household waste.
Must not be disposed together with household garbage. Do not allow product to reach sewage system.
Uncleaned packaging:
Recommendation: Disposal must be made according to official regulations.


* 14 Transport information
Contact Promega Safety Department for additional transport information.
Land transport ADR/RID (cross-border)
ADR/RID class: Not regulated
Maritime transport IMDG:
Marine pollutant: No


15 Regulations
Labelling according to EU guidelines:
Observe the general safety regulations when handling chemicals
The product is not subject to identification regulations under EU Directives and the Ordinance on Hazardous
Materials (German GefStoffV).
National regulations
Waterhazard class: Generally not hazardous for water.


16 Other information
This information is based on our present knowledge. However, this shall not constitute a guarantee for any
specific product features and shall not establish a legally valid contractual relationship.
Department issuing MSDS:
Promega Corporation
Safety Department
2800 Woods Hollow Road
Madison, WI 53711
U.S.A.
(Contd. on page 5)
GB




DR
Page 5/5
Safety Data Sheet
according to 1907/2006/EC, Article 31
Printing date 24.06.2008 Revision: 24.06.2008

Trade name: Biotin Nucleotide Mix

(Contd. of page 4)
Contact:
Promega UK
Delta House, Enterprise Road
Chilworth Science Park
Southampton SO16 7NS
UNITED KINGDOM
Free Phone +44 800 559900
Free Fax +44 800 181037
* Data compared to the previous version altered.
GB




DR
Page 1/5
Safety Data Sheet
according to 1907/2006/EC, Article 31
Printing date 24.06.2008 Revision: 24.06.2008

* 1 Identification of substance
Trade name: Proteinase K
Article number: V302
Application of the substance / the preparation: Laboratory chemicals
Manufacturer/Supplier:
Promega Corporation
2800 Woods Hollow Road
Madison, WI 53711
U.S.A.
1-608-274-4330
1-800-356-9526

MSDS author: Regulatory.Affairs@promega.com
Further information obtainable from:
Promega UK Ltd.
Delta House
Southampton Science Park
Southampton SO16 7NS
UNITED KINGDOM
Tel: (44) 23 8076 0225
Fax: (44) 23 8076 7014
FREEPHONE: 0800 378994
E-mail Address: ukcustserve@promega.com
Web Address: www.promega.com/uk/
Information in case of emergency:
CHEMTREC 1-800-424-9300
For call originating outside the United States dial 001-703-527-3887


* 2 Hazards identification
Hazard description:


Xi Irritant


Information concerning to particular hazards to man and environment
R 36/37/38 Irritating to eyes, respiratory system and skin.
Classification system:
The classification is in line with current EC lists. It is extended, by information from technical literature and
company information.
GB
(Contd. on page 2)




DR
Page 2/5
Safety Data Sheet
according to 1907/2006/EC, Article 31
Printing date 24.06.2008 Revision: 24.06.2008

Trade name: Proteinase K

(Contd. of page 1)


* 3 Composition/information on ingredients
Chemical characterization:
CAS No. Description:
39450-01-6 Proteinase, Tritirachium album serine
Identification number(s):
EEC Number: 254-457-8


4 First aid measures
After inhalation: In case of unconsciousness place patient stably in side position for transportation.
After skin contact: Immediately wash with soap, water and rinse thoroughly.
After eye contact:
Rinse opened eye for several minutes under running water. If symptoms persist, consult a doctor.
After swallowing: If symptoms persist consult doctor.


5 Fire fighting measures
Suitable extinguishing agents:
CO2, powder or water spray. Fight larger fires with water spray or alcohol resistant foam.


* 6 Accidental release measures
Person-related safety precautions: Not required.
Measures for environmental protection: No special measures required.
Measures for cleaning/collecting: Ensure adequate ventilation.


7 Handling and storage
Handling
Information for safe handling: No special precautions are necessary if used correctly.
Information about fire - and explosion protection: No special measures required.
Storage
Requirements to be met by storerooms and receptacles: No special requirements.
Information about storage in one common storage facility: Not required.
Further information about storage conditions: Keep receptacle tightly sealed.


* 8 Exposure controls and personal protection gear
Additional information about design of technical facilities: No further data; see item 7.
Ingredients with limit values that require monitoring at the workplace: Not required.
Additional information: The lists valid during the making were used as basis.
Personal protective equipment
General protective and hygienic measures
Keep away from foodstuffs, beverages and feed.
Immediately remove all soiled and contaminated clothing
Wash hands before breaks and at the end of work.
(Contd. on page 3)
GB




DR
Page 3/5
Safety Data Sheet
according to 1907/2006/EC, Article 31
Printing date 24.06.2008 Revision: 24.06.2008

Trade name: Proteinase K

(Contd. of page 2)
Avoid contact with the eyes and skin.
Respiratory protection:
In case of brief exposure or low pollution use respiratory filter device. In case of intensive or longer exposure use
self-contained respiratory protective device.
Protection of hands:
Protective gloves.
Selection of the glove material on consideration of the penetration times, rates of diffusion and the degradation
Material of gloves:
The selection of the suitable gloves does not only depend on the material, but also on further marks of quality and
varies from manufacturer to manufacturer.
Penetration time of glove material:
The exact break trough time has to be found out by the manufacturer of the protective gloves and has to be
observed.
Eye protection: Tightly sealed goggles.


* 9 Physical and chemical properties
General Information
Solid.
Form:
Colourless
Colour:
Characteristic
Odour:
Change in condition
Melting point/Melting range: undetermined
Boiling point/Boiling range: undetermined
Not applicable
Flash point:
Flammability (solid, gaseous) Product is not flammable.
Product does not present an explosion hazard.
Danger of explosion:
1.1 g/cm?br> Density at 20癈:
Solubility in / Miscibility with
Slightly soluble
Water:
0.0 %
Organic solvents:



10 Stability and reactivity
Thermal decomposition / conditions to be avoided: No decomposition if used according to specifications.
Dangerous reactions: Reacts with strong oxidizing agents
Dangerous decomposition products: Carbon monoxide and carbon dioxide


* 11 Toxicological information
Acute toxicity:
Primary irritant effect:
on the skin: Irritant to skin and mucous membranes.
on the eye: Irritating effect.
(Contd. on page 4)
GB




DR
Page 4/5
Safety Data Sheet
according to 1907/2006/EC, Article 31
Printing date 24.06.2008 Revision: 24.06.2008

Trade name: Proteinase K

(Contd. of page 3)
Sensitization: No sensitizing effects known.


12 Ecological information
General notes: Generally not hazardous for water.


13 Disposal considerations
Product:
Recommendation
Must not be disposed together with household garbage. Do not allow product to reach sewage system.
Uncleaned packaging:
Recommendation: Disposal must be made according to official regulations.
Recommended cleansing agents: Water, if necessary together with cleansing agents.


* 14 Transport information
Contact Promega Safety Department for additional transport information.
Land transport ADR/RID (cross-border)
ADR/RID class: -
Not regulated
Maritime transport IMDG:
-
IMDG Class:
Marine pollutant: No
Air transport ICAO-TI and IATA-DGR:
ICAO/IATA Class: -


* 15 Regulations
Labelling according to EU guidelines:
The product has been classified and marked in accordance with EU Directives / Ordinance on Hazardous
Materials
Risk phrases:
36/37/38 Irritating to eyes, respiratory system and skin.
Safety phrases:
26 In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
National regulations
Technical instructions (air):
Class Share in %
I 100.0

Waterhazard class: Generally not hazardous for water.
GB
(Contd. on page 5)




DR
Page 5/5
Safety Data Sheet
according to 1907/2006/EC, Article 31
Printing date 24.06.2008 Revision: 24.06.2008

Trade name: Proteinase K

(Contd. of page 4)


16 Other information
This information is based on our present knowledge. However, this shall not constitute a guarantee for any
specific product features and shall not establish a legally valid contractual relationship.
Department issuing MSDS:
Promega Corporation
Safety Department
2800 Woods Hollow Road
Madison, WI 53711
U.S.A.
Contact:
Promega UK
Delta House, Enterprise Road
Chilworth Science Park
Southampton SO16 7NS
UNITED KINGDOM
Free Phone +44 800 559900
Free Fax +44 800 181037
* Data compared to the previous version altered.
GB




DR
Page 1/5
Safety Data Sheet
according to 1907/2006/EC, Article 31
Printing date 24.06.2008 Revision: 24.06.2008

* 1 Identification of substance
Trade name: SSC, 20X
Article number: G329
Application of the substance / the preparation: Laboratory chemicals
Manufacturer/Supplier:
Promega Corporation
2800 Woods Hollow Road
Madison, WI 53711
U.S.A.
1-608-274-4330
1-800-356-9526

MSDS author: Regulatory.Affairs@promega.com
Further information obtainable from:
Promega UK Ltd.
Delta House
Southampton Science Park
Southampton SO16 7NS
UNITED KINGDOM
Tel: (44) 23 8076 0225
Fax: (44) 23 8076 7014
FREEPHONE: 0800 378994
E-mail Address: ukcustserve@promega.com
Web Address: www.promega.com/uk/
Information in case of emergency:
CHEMTREC 1-800-424-9300
For call originating outside the United States dial 001-703-527-3887


2 Hazards identification
Hazard description: not applicable
Information concerning to particular hazards to man and environment
The product does not have to be labelled due to the calculation procedure of the "General Classification
guideline for preparations of the EU" in the latest valid version.
Classification system:
The classification is in line with current EC lists. It is extended, by information from technical literature and
company information.


3 Composition/information on ingredients
Chemical characterization
Description: Mixture of substances listed below with nonhazardous additions.
Components: Void
(Contd. on page 2)
GB




DR
Page 2/5
Safety Data Sheet
according to 1907/2006/EC, Article 31
Printing date 24.06.2008 Revision: 24.06.2008

Trade name: SSC, 20X

(Contd. of page 1)
Additional information: For the wording of the listed risk phrases refer to section 16.


4 First aid measures
General information: No special measures required.
After inhalation: Supply fresh air; consult doctor in case of complaints.
After skin contact: Generally the product does not irritate the skin.
After eye contact: Rinse opened eye for several minutes under running water.
After swallowing: If symptoms persist consult doctor.


5 Fire fighting measures
Suitable extinguishing agents:
CO2, powder or water spray. Fight larger fires with water spray or alcohol resistant foam.
Protective equipment: No special measures required.


6 Accidental release measures
Person-related safety precautions: Not required.
Measures for environmental protection:
Dilute with plenty of water.
Do not allow to enter sewers/ surface or ground water.
Measures for cleaning/collecting:
Absorb with liquid-binding material (sand, diatomite, acid binders, universal binders, sawdust).
Additional information: No dangerous substances are released.


7 Handling and storage
Handling
Information for safe handling: No special measures required.
Information about fire - and explosion protection: No special measures required.
Storage
Requirements to be met by storerooms and receptacles: No special requirements.
Information about storage in one common storage facility: Not required.
Further information about storage conditions: None.


8 Exposure controls and personal protection gear
Additional information about design of technical facilities: No further data; see item 7.
Ingredients with limit values that require monitoring at the workplace:
The product does not contain any relevant quantities of materials with critical values that have to be monitored at
the workplace.
Additional information: The lists valid during the making were used as basis.
Personal protective equipment
General protective and hygienic measures
The usual precautionary measures are to be adhered to when handling chemicals.
Respiratory protection: Not required.
(Contd. on page 3)
GB




DR
Page 3/5
Safety Data Sheet
according to 1907/2006/EC, Article 31
Printing date 24.06.2008 Revision: 24.06.2008

Trade name: SSC, 20X

(Contd. of page 2)
Protection of hands:
Protective gloves.
Selection of the glove material on consideration of the penetration times, rates of diffusion and the degradation
Material of gloves:
The selection of the suitable gloves does not only depend on the material, but also on further marks of quality and
varies from manufacturer to manufacturer. As the product is a preparation of several substances, the resistance
of the glove material can not be calculated in advance and has therefore to be checked prior to the application.
Penetration time of glove material:
The exact break trough time has to be found out by the manufacturer of the protective gloves and has to be
observed.
Eye protection: Goggles recommended during refilling.


9 Physical and chemical properties
General Information
Fluid
Form:
Colourless
Colour:
Characteristic
Odour:
Change in condition
Melting point/Melting range: undetermined
Boiling point/Boiling range: 100癈
Not applicable
Flash point:
Product is not selfigniting.
Self-igniting:
Product does not present an explosion hazard.
Danger of explosion:
1.165 g/cm?br> Density at 20癈:
Solubility in / Miscibility with
Fully miscible
Water:
0
pH-value at 20癈:
Solvent content:
0.0 %
Organic solvents:
74.5 %
Water:
26.4 %
Solids content:



10 Stability and reactivity
Thermal decomposition / conditions to be avoided: No decomposition if used according to specifications.
Dangerous reactions: No dangerous reactions known
Dangerous decomposition products: No dangerous decomposition products known


11 Toxicological information
Acute toxicity:
Primary irritant effect:
on the skin: No irritant effect.
on the eye: Irritating effect.
(Contd. on page 4)
GB




DR
Page 4/5
Safety Data Sheet
according to 1907/2006/EC, Article 31
Printing date 24.06.2008 Revision: 24.06.2008

Trade name: SSC, 20X

(Contd. of page 3)
Sensitization: No sensitizing effects known.
Additional toxicological information:
The product is not subject to classification according to the calculation method of the General EU Classification
Guidelines for Preparations as issued in the latest version.
When used and handled according to specifications, the product does not have any harmful effects to our
experience and the information provided to us.


12 Ecological information
General notes:
Water hazard class 1 (German Regulation) (Self-assessment): slightly hazardous for water.
Do not allow undiluted product or large quantities of it to reach ground water, water course or sewage system.
Rinse off of bigger amounts into drains or the aquatic environment may lead to decreased pH-values. A low pH-
value harms aquatic organisms. In the dilution of the use-level the pH-value is considerably increased, so that
after the use of the product the aqueous waste, emptied into drains, is only low water-dangerous.


13 Disposal considerations
Product:
Recommendation
Smaller quantities can be disposed of with household waste.
Must not be disposed together with household garbage. Do not allow product to reach sewage system.
Uncleaned packaging:
Recommendation: Disposal must be made according to official regulations.
Recommended cleansing agents: Water, if necessary together with cleansing agents.


* 14 Transport information
Contact Promega Safety Department for additional transport information.
Land transport ADR/RID (cross-border)
ADR/RID class: -
Not regulated
Maritime transport IMDG:
-
IMDG Class:
Marine pollutant: No
Air transport ICAO-TI and IATA-DGR:
ICAO/IATA Class: -



15 Regulations
Labelling according to EU guidelines:
Observe the general safety regulations when handling chemicals
The product is not subject to identification regulations under EU Directives and the Ordinance on Hazardous
Materials (German GefStoffV).
(Contd. on page 5)
GB




DR
Page 5/5
Safety Data Sheet
according to 1907/2006/EC, Article 31
Printing date 24.06.2008 Revision: 24.06.2008

Trade name: SSC, 20X

(Contd. of page 4)

National regulations
Waterhazard class: Water hazard class 1 (Self-assessment): slightly hazardous for water.


16 Other information
This information is based on our present knowledge. However, this shall not constitute a guarantee for any
specific product features and shall not establish a legally valid contractual relationship.
Department issuing MSDS:
Promega Corporation
Safety Department
2800 Woods Hollow Road
Madison, WI 53711
U.S.A.
Contact:
Promega UK
Delta House, Enterprise Road
Chilworth Science Park
Southampton SO16 7NS
UNITED KINGDOM
Free Phone +44 800 559900
Free Fax +44 800 181037
* Data compared to the previous version altered.
GB




DR
Page 1/5
Safety Data Sheet
according to 1907/2006/EC, Article 31
Printing date 24.06.2008 Revision: 24.06.2008

* 1 Identification of substance
Trade name: DAB 20X Chromogen
Article number: G716
Application of the substance / the preparation: Laboratory chemicals
Manufacturer/Supplier:
Promega Corporation
2800 Woods Hollow Road
Madison, WI 53711
U.S.A.
1-608-274-4330
1-800-356-9526

MSDS author: Regulatory.Affairs@promega.com
Further information obtainable from:
Promega UK Ltd.
Delta House
Southampton Science Park
Southampton SO16 7NS
UNITED KINGDOM
Tel: (44) 23 8076 0225
Fax: (44) 23 8076 7014
FREEPHONE: 0800 378994
E-mail Address: ukcustserve@promega.com
Web Address: www.promega.com/uk/
Information in case of emergency:
CHEMTREC 1-800-424-9300
For call originating outside the United States dial 001-703-527-3887


2 Hazards identification
Hazard description: not applicable
Information concerning to particular hazards to man and environment
The product does not have to be labelled due to the calculation procedure of the "General Classification
guideline for preparations of the EU" in the latest valid version.
Classification system:
The classification is in line with current EC lists. It is extended, by information from technical literature and
company information.


3 Composition/information on ingredients
Chemical characterization
Description: Mixture of substances listed below with nonhazardous additions.
(Contd. on page 2)
GB




DR
Page 2/5
Safety Data Sheet
according to 1907/2006/EC, Article 31
Printing date 24.06.2008 Revision: 24.06.2008

Trade name: DAB 20X Chromogen

(Contd. of page 1)
Components:
CAS: 7411-49-6 biphenyl-3,3',4,4'-tetrayltetraammoniumtetrachloride Xn; R 20/22 <2.00%
EINECS: 231-018-9
Additional information: For the wording of the listed risk phrases refer to section 16.


4 First aid measures
General information: No special measures required.
After inhalation: Supply fresh air; consult doctor in case of complaints.
After skin contact: Generally the product does not irritate the skin.
After eye contact: Rinse opened eye for several minutes under running water.
After swallowing: If symptoms persist consult doctor.


5 Fire fighting measures
Suitable extinguishing agents:
CO2, powder or water spray. Fight larger fires with water spray or alcohol resistant foam.
Protective equipment: No special measures required.


6 Accidental release measures
Person-related safety precautions: Not required.
Measures for environmental protection: No special measures required.
Measures for cleaning/collecting:
Absorb with liquid-binding material (sand, diatomite, acid binders, universal binders, sawdust).
Additional information: No dangerous substances are released.


7 Handling and storage
Handling
Information for safe handling: No special measures required.
Information about fire - and explosion protection: No special measures required.
Storage
Requirements to be met by storerooms and receptacles: No special requirements.
Information about storage in one common storage facility: Not required.
Further information about storage conditions: None.


8 Exposure controls and personal protection gear
Additional information about design of technical facilities: No further data; see item 7.
Ingredients with limit values that require monitoring at the workplace:
The product does not contain any relevant quantities of materials with critical values that have to be monitored at
the workplace.
Additional information: The lists valid during the making were used as basis.
Personal protective equipment
General protective and hygienic measures
The usual precautionary measures are to be adhered to when handling chemicals.
(Contd. on page 3)
GB




DR
Page 3/5
Safety Data Sheet
according to 1907/2006/EC, Article 31
Printing date 24.06.2008 Revision: 24.06.2008

Trade name: DAB 20X Chromogen

(Contd. of page 2)
Respiratory protection: Not required.
Protection of hands:
Protective gloves.
Selection of the glove material on consideration of the penetration times, rates of diffusion and the degradation
Material of gloves:
The selection of the suitable gloves does not only depend on the material, but also on further marks of quality and
varies from manufacturer to manufacturer. As the product is a preparation of several substances, the resistance
of the glove material can not be calculated in advance and has therefore to be checked prior to the application.
Penetration time of glove material:
The exact break trough time has to be found out by the manufacturer of the protective gloves and has to be
observed.
Eye protection: Goggles recommended during refilling.


9 Physical and chemical properties
General Information
Fluid
Form:
Colourless
Colour:
Characteristic
Odour:
Change in condition
Melting point/Melting range: 0癈
Boiling point/Boiling range: 100癈
Not applicable
Flash point:
Product is not selfigniting.
Self-igniting:
Product does not present an explosion hazard.
Danger of explosion:
1 g/cm?br> Density at 20癈:
Solubility in / Miscibility with
Not miscible or difficult to mix
Water:
Solvent content:
0.0 %
Organic solvents:
98.0 %
Water:
2.0 %
Solids content:



10 Stability and reactivity
Thermal decomposition / conditions to be avoided: No decomposition if used according to specifications.
Dangerous reactions: No dangerous reactions known
Dangerous decomposition products: No dangerous decomposition products known


11 Toxicological information
Acute toxicity:
Primary irritant effect:
on the skin: No irritant effect.
on the eye: Irritating effect.
Sensitization: No sensitizing effects known.
(Contd. on page 4)
GB




DR
Page 4/5
Safety Data Sheet
according to 1907/2006/EC, Article 31
Printing date 24.06.2008 Revision: 24.06.2008

Trade name: DAB 20X Chromogen

(Contd. of page 3)
Additional toxicological information:
The product is not subject to classification according to the calculation method of the General EU Classification
Guidelines for Preparations as issued in the latest version.
When used and handled according to specifications, the product does not have any harmful effects to our
experience and the information provided to us.


12 Ecological information
General notes: Generally not hazardous for water.


13 Disposal considerations
Product:
Recommendation
Smaller quantities can be disposed of with household waste.
Must not be disposed together with household garbage. Do not allow product to reach sewage system.
Uncleaned packaging:
Recommendation: Disposal must be made according to official regulations.


* 14 Transport information
Contact Promega Safety Department for additional transport information.
Land transport ADR/RID (cross-border)
ADR/RID class: -
Not regulated
Maritime transport IMDG:
-
IMDG Class:
Marine pollutant: No
Air transport ICAO-TI and IATA-DGR:
ICAO/IATA Class: -



15 Regulations
Labelling according to EU guidelines:
Observe the general safety regulations when handling chemicals
The product is not subject to identification regulations under EU Directives and the Ordinance on Hazardous
Materials (German GefStoffV).
Special labelling of certain preparations:
Safety data sheet available for professional user on request.
National regulations
Waterhazard class: Generally not hazardous for water.
GB
(Contd. on page 5)




DR
Page 5/5
Safety Data Sheet
according to 1907/2006/EC, Article 31
Printing date 24.06.2008 Revision: 24.06.2008

Trade name: DAB 20X Chromogen

(Contd. of page 4)


16 Other information
This information is based on our present knowledge. However, this shall not constitute a guarantee for any
specific product features and shall not establish a legally valid contractual relationship.
Relevant R-phrases
20/22 Harmful by inhalation and if swallowed.
Department issuing MSDS:
Promega Corporation
Safety Department
2800 Woods Hollow Road
Madison, WI 53711
U.S.A.
Contact:
Promega UK
Delta House, Enterprise Road
Chilworth Science Park
Southampton SO16 7NS
UNITED KINGDOM
Free Phone +44 800 559900
Free Fax +44 800 181037
* Data compared to the previous version altered.
GB




DR
Page 1/5
Safety Data Sheet
according to 1907/2006/EC, Article 31
Printing date 24.06.2008 Revision: 24.06.2008

* 1 Identification of substance
Trade name: DAB 20X Buffer
Article number: G717
Application of the substance / the preparation: Laboratory chemicals
Manufacturer/Supplier:
Promega Corporation
2800 Woods Hollow Road
Madison, WI 53711
U.S.A.
1-608-274-4330
1-800-356-9526

MSDS author: Regulatory.Affairs@promega.com
Further information obtainable from:
Promega UK Ltd.
Delta House
Southampton Science Park
Southampton SO16 7NS
UNITED KINGDOM
Tel: (44) 23 8076 0225
Fax: (44) 23 8076 7014
FREEPHONE: 0800 378994
E-mail Address: ukcustserve@promega.com
Web Address: www.promega.com/uk/
Information in case of emergency:
CHEMTREC 1-800-424-9300
For call originating outside the United States dial 001-703-527-3887


2 Hazards identification
Hazard description: not applicable
Information concerning to particular hazards to man and environment
The product does not have to be labelled due to the calculation procedure of the "General Classification
guideline for preparations of the EU" in the latest valid version.
Classification system:
The classification is in line with current EC lists. It is extended, by information from technical literature and
company information.


3 Composition/information on ingredients
Chemical characterization
Description: Mixture of substances listed below with nonhazardous additions.
(Contd. on page 2)
GB




DR
Page 2/5
Safety Data Sheet
according to 1907/2006/EC, Article 31
Printing date 24.06.2008 Revision: 24.06.2008

Trade name: DAB 20X Buffer

(Contd. of page 1)
Components:
CAS: 1185-53-1 Tris(hydroxymethyl)aminomethane hydrochloride Xi; R 36/37/38 10-15%
EINECS: 214-684-5
CAS: 77-86-1 2-Amino-2-(hydroxymethyl)-1,3-propanediol Xi; R 36/37/38 1.0-5.0%
EINECS: 201-064-4
Additional information: For the wording of the listed risk phrases refer to section 16.


4 First aid measures
General information: No special measures required.
After inhalation: Supply fresh air; consult doctor in case of complaints.
After skin contact: Generally the product does not irritate the skin.
After eye contact: Rinse opened eye for several minutes under running water.
After swallowing: If symptoms persist consult doctor.


5 Fire fighting measures
Suitable extinguishing agents:
CO2, powder or water spray. Fight larger fires with water spray or alcohol resistant foam.
Protective equipment: No special measures required.


6 Accidental release measures
Person-related safety precautions: Not required.
Measures for environmental protection: Dilute with plenty of water.
Measures for cleaning/collecting:
Absorb with liquid-binding material (sand, diatomite, acid binders, universal binders, sawdust).
Additional information: No dangerous substances are released.


7 Handling and storage
Handling
Information for safe handling: No special measures required.
Information about fire - and explosion protection: No special measures required.
Storage
Requirements to be met by storerooms and receptacles: No special requirements.
Information about storage in one common storage facility: Not required.
Further information about storage conditions: None.


8 Exposure controls and personal protection gear
Additional information about design of technical facilities: No further data; see item 7.
Ingredients with limit values that require monitoring at the workplace:
The product does not contain any relevant quantities of materials with critical values that have to be monitored at
the workplace.
Additional information: The lists valid during the making were used as basis.
(Contd. on page 3)
GB




DR
Page 3/5
Safety Data Sheet
according to 1907/2006/EC, Article 31
Printing date 24.06.2008 Revision: 24.06.2008

Trade name: DAB 20X Buffer

(Contd. of page 2)

Personal protective equipment
General protective and hygienic measures
The usual precautionary measures are to be adhered to when handling chemicals.
Respiratory protection: Not required.
Protection of hands:
Protective gloves.
Selection of the glove material on consideration of the penetration times, rates of diffusion and the degradation
Material of gloves:
The selection of the suitable gloves does not only depend on the material, but also on further marks of quality and
varies from manufacturer to manufacturer. As the product is a preparation of several substances, the resistance
of the glove material can not be calculated in advance and has therefore to be checked prior to the application.
Penetration time of glove material:
The exact break trough time has to be found out by the manufacturer of the protective gloves and has to be
observed.
Eye protection: Goggles recommended during refilling.


9 Physical and chemical properties
General Information
Fluid
Form:
Colourless
Colour:
Characteristic
Odour:
Change in condition
Melting point/Melting range: undetermined
Boiling point/Boiling range: 100癈
Not applicable
Flash point:
Product is not selfigniting.
Self-igniting:
Product does not present an explosion hazard.
Danger of explosion:
Not determined
Density:
Solubility in / Miscibility with
Fully miscible
Water:
Solvent content:
0.0 %
Organic solvents:
82.0 %
Water:
10.0 %
Solids content:



10 Stability and reactivity
Thermal decomposition / conditions to be avoided: No decomposition if used according to specifications.
Dangerous reactions: No dangerous reactions known
Dangerous decomposition products: No dangerous decomposition products known
GB
(Contd. on page 4)




DR
Page 4/5
Safety Data Sheet
according to 1907/2006/EC, Article 31
Printing date 24.06.2008 Revision: 24.06.2008

Trade name: DAB 20X Buffer

(Contd. of page 3)


11 Toxicological information
Acute toxicity:
Primary irritant effect:
on the skin: No irritant effect.
on the eye: Irritating effect.
Sensitization: No sensitizing effects known.
Additional toxicological information:
The product is not subject to classification according to the calculation method of the General EU Classification
Guidelines for Preparations as issued in the latest version.
When used and handled according to specifications, the product does not have any harmful effects to our
experience and the information provided to us.


12 Ecological information
General notes: Generally not hazardous for water.


13 Disposal considerations
Product:
Recommendation
Smaller quantities can be disposed of with household waste.
Must not be disposed together with household garbage. Do not allow product to reach sewage system.
Uncleaned packaging:
Recommendation: Disposal must be made according to official regulations.
Recommended cleansing agents: Water, if necessary together with cleansing agents.


* 14 Transport information
Contact Promega Safety Department for additional transport information.
Land transport ADR/RID (cross-border)
ADR/RID class: Not regulated
Maritime transport IMDG:
Marine pollutant: No


15 Regulations
Labelling according to EU guidelines:
Observe the general safety regulations when handling chemicals
The product is not subject to identification regulations under EU Directives and the Ordinance on Hazardous
Materials (German GefStoffV).
Special labelling of certain preparations:
Safety data sheet available for professional user on request.
National regulations
Waterhazard class: Generally not hazardous for water.
GB
(Contd. on page 5)




DR
Page 5/5
Safety Data Sheet
according to 1907/2006/EC, Article 31
Printing date 24.06.2008 Revision: 24.06.2008

Trade name: DAB 20X Buffer

(Contd. of page 4)


16 Other information
This information is based on our present knowledge. However, this shall not constitute a guarantee for any
specific product features and shall not establish a legally valid contractual relationship.
Relevant R-phrases
36/37/38 Irritating to eyes, respiratory system and skin.
Department issuing MSDS:
Promega Corporation
Safety Department
2800 Woods Hollow Road
Madison, WI 53711
U.S.A.
Contact:
Promega UK
Delta House, Enterprise Road
Chilworth Science Park
Southampton SO16 7NS
UNITED KINGDOM
Free Phone +44 800 559900
Free Fax +44 800 181037
* Data compared to the previous version altered.
GB




DR
Page 1/5
Safety Data Sheet
according to 1907/2006/EC, Article 31
Printing date 24.06.2008 Revision: 24.06.2008

* 1 Identification of substance
Trade name: Hydrogen Peroxide 20X
Article number: G718
Application of the substance / the preparation: Laboratory chemicals
Manufacturer/Supplier:
Promega Corporation
2800 Woods Hollow Road
Madison, WI 53711
U.S.A.
1-608-274-4330
1-800-356-9526

MSDS author: Regulatory.Affairs@promega.com
Further information obtainable from:
Promega UK Ltd.
Delta House
Southampton Science Park
Southampton SO16 7NS
UNITED KINGDOM
Tel: (44) 23 8076 0225
Fax: (44) 23 8076 7014
FREEPHONE: 0800 378994
E-mail Address: ukcustserve@promega.com
Web Address: www.promega.com/uk/
Information in case of emergency:
CHEMTREC 1-800-424-9300
For call originating outside the United States dial 001-703-527-3887


2 Hazards identification
Hazard description: not applicable
Information concerning to particular hazards to man and environment
The product does not have to be labelled due to the calculation procedure of the "General Classification
guideline for preparations of the EU" in the latest valid version.
Classification system:
The classification is in line with current EC lists. It is extended, by information from technical literature and
company information.


3 Composition/information on ingredients
Chemical characterization
Description: Mixture of substances listed below with nonhazardous additions.
Components: Void
(Contd. on page 2)
GB




DR
Page 2/5
Safety Data Sheet
according to 1907/2006/EC, Article 31
Printing date 24.06.2008 Revision: 24.06.2008

Trade name: Hydrogen Peroxide 20X

(Contd. of page 1)
Additional information: For the wording of the listed risk phrases refer to section 16.


4 First aid measures
General information: No special measures required.
After inhalation: Supply fresh air; consult doctor in case of complaints.
After skin contact: Generally the product does not irritate the skin.
After eye contact: Rinse opened eye for several minutes under running water.
After swallowing: If symptoms persist consult doctor.


5 Fire fighting measures
Suitable extinguishing agents:
CO2, powder or water spray. Fight larger fires with water spray or alcohol resistant foam.
Protective equipment: No special measures required.


6 Accidental release measures
Person-related safety precautions: Not required.
Measures for environmental protection: Dilute with plenty of water.
Measures for cleaning/collecting:
Absorb with liquid-binding material (sand, diatomite, acid binders, universal binders, sawdust).
Additional information: No dangerous substances are released.


7 Handling and storage
Handling
Information for safe handling: No special measures required.
Information about fire - and explosion protection: No special measures required.
Storage
Requirements to be met by storerooms and receptacles: No special requirements.
Information about storage in one common storage facility: Not required.
Further information about storage conditions: None.


8 Exposure controls and personal protection gear
Additional information about design of technical facilities: No further data; see item 7.
Ingredients with limit values that require monitoring at the workplace:
The product does not contain any relevant quantities of materials with critical values that have to be monitored at
the workplace.
Additional information: The lists valid during the making were used as basis.
Personal protective equipment
General protective and hygienic measures
The usual precautionary measures are to be adhered to when handling chemicals.
Respiratory protection: Not required.
Protection of hands:
Protective gloves.
Selection of the glove material on consideration of the penetration times, rates of diffusion and the degradation
(Contd. on page 3)
GB




DR
Page 3/5
Safety Data Sheet
according to 1907/2006/EC, Article 31
Printing date 24.06.2008 Revision: 24.06.2008

Trade name: Hydrogen Peroxide 20X

(Contd. of page 2)
Material of gloves:
The selection of the suitable gloves does not only depend on the material, but also on further marks of quality and
varies from manufacturer to manufacturer. As the product is a preparation of several substances, the resistance
of the glove material can not be calculated in advance and has therefore to be checked prior to the application.
Penetration time of glove material:
The exact break trough time has to be found out by the manufacturer of the protective gloves and has to be
observed.
Eye protection: Goggles recommended during refilling.


9 Physical and chemical properties
General Information
Fluid
Form:
Colourless
Colour:
Characteristic
Odour:
Change in condition
Melting point/Melting range: 0癈
Boiling point/Boiling range: 100癈
Not applicable
Flash point:
Product is not selfigniting.
Self-igniting:
Product does not present an explosion hazard.
Danger of explosion:
1 g/cm?br> Density at 20癈:
Solubility in / Miscibility with
Fully miscible
Water:
Solvent content:
0.0 %
Organic solvents:
99.4 %
Water:



10 Stability and reactivity
Thermal decomposition / conditions to be avoided: No decomposition if used according to specifications.
Dangerous reactions: No dangerous reactions known
Dangerous decomposition products: No dangerous decomposition products known


11 Toxicological information
Acute toxicity:
Primary irritant effect:
on the skin: No irritant effect.
on the eye: Irritating effect.
Sensitization: No sensitizing effects known.
Additional toxicological information:
The product is not subject to classification according to the calculation method of the General EU Classification
Guidelines for Preparations as issued in the latest version.
(Contd. on page 4)
GB




DR
Page 4/5
Safety Data Sheet
according to 1907/2006/EC, Article 31
Printing date 24.06.2008 Revision: 24.06.2008

Trade name: Hydrogen Peroxide 20X

(Contd. of page 3)
When used and handled according to specifications, the product does not have any harmful effects to our
experience and the information provided to us.


12 Ecological information
General notes: Generally not hazardous for water.


13 Disposal considerations
Product:
Recommendation
Smaller quantities can be disposed of with household waste.
Must not be disposed together with household garbage. Do not allow product to reach sewage system.
Uncleaned packaging:
Recommendation: Disposal must be made according to official regulations.
Recommended cleansing agents: Water, if necessary together with cleansing agents.


* 14 Transport information
Contact Promega Safety Department for additional transport information.
Land transport ADR/RID (cross-border)
ADR/RID class: Not regulated
Maritime transport IMDG:
Marine pollutant: No


15 Regulations
Labelling according to EU guidelines:
Observe the general safety regulations when handling chemicals
The product is not subject to identification regulations under EU Directives and the Ordinance on Hazardous
Materials (German GefStoffV).
National regulations
Waterhazard class: Generally not hazardous for water.


16 Other information
This information is based on our present knowledge. However, this shall not constitute a guarantee for any
specific product features and shall not establish a legally valid contractual relationship.
Department issuing MSDS:
Promega Corporation
Safety Department
2800 Woods Hollow Road
Madison, WI 53711
U.S.A.
(Contd. on page 5)
GB




DR
Page 5/5
Safety Data Sheet
according to 1907/2006/EC, Article 31
Printing date 24.06.2008 Revision: 24.06.2008

Trade name: Hydrogen Peroxide 20X

(Contd. of page 4)
Contact:
Promega UK
Delta House, Enterprise Road
Chilworth Science Park
Southampton SO16 7NS
UNITED KINGDOM
Free Phone +44 800 559900
Free Fax +44 800 181037
* Data compared to the previous version altered.
GB




DR

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