TEPNEL LIFECODES MOLECULAR DIAGNOSTICS
550 West Avenue ?Stamford, CT 06902
203.328.9500 ?888.329.0255 ?203.328.9598 fax
MATERIAL SAFETY DATA SHEET
(In compliance with 29 CFR 1910.1200 and based on Directive 91/155 EEC et seq.)
Product Name: LIFECODES HLA-SSO DNA Typing Kits. For Use With Luminex
(HLA-A, HLA-B, HLA-C, HLA-DRB, HLA-DRB1, HLA-DQB, HLA-DQA, HLA-DPB,
KIR, CYTOKINE)
1. INFORMATION OF THE SUBSTANCE/PREPARATION AND COMPANY
1.1 Date: April 20, 2009
Product Name: LIFECODES HLA-SSO DNA Typing Kits. For Use With Luminex
Intended Use: DNA Typing of Class I and Class II HLA alleles
Catalog Numbers: HLA-A: 628410-50
HLA-B: 628510-50
HLA-C: 628810-50
HLA-DRB: 628710-50
HLA-DRB1: 628751-50
HLA-DQB: 628610-50, 628610M
HLA-DQA: 628061
HLA-DPB: 628910
KIR: 545110
CYTOKINE: 345110
Kit Components:
Kit Primer Mix/Master Mix Probe Mix Dilution Solution
HLA-A HLA-A Probe Mix
HLA-A Master Mix (870礚) 9.9mL
(810礚)
HLA-B HLA-B Probe Mix
HLA-B Master Mix (870礚) 9.9mL
(810礚)
HLA-C HLA-C Probe Mix
HLA-C Master Mix (870礚) 9.9mL
(810礚)
HLA-DRB DRB Generic Master Mix
(870 礚) HLA-DRB Probe Mix
19.7mL
DR52 Master Mix (810礚 x2)
(870 礚)
HLA-DRB1 HLA-DRB1 Master Mix HLA-DRB1 Probe Mix
9.9 mL
(870uL) (810 礚)
HLA-DQB HLA-DQB Master Mix HLA-DQB Probe Mix
9.9mL
(870礚) (810礚)
HLA-DQB HLA-DQB Master Mix HLA-DQB Probe Mix
23.8mL
(Mega Kit) (1050 礚 x 2 vials) (1050 礚 x 2 vials)
HLA-DQA HLA-DQA Master Mix HLA-DQA Probe Mix
9.9mL
(420礚) (360礚)
HLA-DPB HLA-DPB Master Mix HLA-DPB Probe Mix
9.9mL
(420礚) (360礚)
KIR KIR 1 Master Mix (170礚) KIR Probe Mix
9.9mL
KIR 2 Master Mix (170礚) (720礚)
CYTOKINE Cytokine I Master Mix Cytokine I Probe Mix
(225礚) (360礚)
Cytokine II Master Mix Cytokine II Probe Mix
19.7mL
(225礚) (360礚)
TGFb Master Mix TGFb Probe Mix
(225礚) (360礚)
LC802.11
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TEPNEL LIFECODES MOLECULAR DIAGNOSTICS
550 West Avenue ?Stamford, CT 06902
203.328.9500 ?888.329.0255 ?203.328.9598 fax
1.2 Company Tepnel Lifecodes
550 West Avenue
Stamford, CT 06902 USA
(203) 328-9500
technicalsupport@tepnellifecodes.com
1.3 In emergencies: CHEMTREC 1-800-424-9300 (24 Hours, 7 days/week)
2. HAZARDS IDENTIFICATION
The hazards identified with this product are those associated with tetramethylammonium chloride.
Tetramethylammonium chloride is a slight irritant and poison by ingestion. Potential Health Effects include:
Inhalation may cause irritation, Eye Contact may cause irritation, Skin contact may cause irritation, Ingestion
may cause dizziness, nausea and shortness of breath (nicotine-like symptoms). Acts as a ganglionic blocking
agent and vasodilator. May be fatal is swallowed. Chronic effects may cause irritation.
Exposure Limits 8 hrs. TWA
OSHA PEL ACGIH TLV Supplier rec.
None established None established None established
NFPA HAZARD IDENTIFICATION RATING SYSTEM
HEALTH: 3 FIRE: 0 REACTIVITY: 0
EMERGENCY OVERVIEW:
Wear personal protective equipment to prevent contact with eyes. May cause skin, respiratory tract and eye
irritation. May be fatal if swallowed. Remove and wash all contaminated clothing before re-use. Thoroughly
wash effected area with water.
3. COMPONENTS/INFORMATION ON INGREDIENTS
Probe Mix
Tetramethylammonium chloride CAS Registry # 75-57-0
All other components are confidential as per the trade secret provision of 29 CFR 1910.1200.
4. FIRST AID MEASURES:
Probe Mix:
Inhalation: Remove to fresh air, if breathing has stopped, give artificial respiration and consult a physician.
Eye Contact: Immediately flush with water until no evidence of chemical remains (at least 15 minutes) and
consult a physician. Skin Contact: Immediately flush with water with sufficient volume until there is no
evidence of the chemical on the affected area. Ingestion: may cause dizziness, nausea and shortness of breath
(nicotine-like symptoms). Acts as a ganglionic blocking agent and vasodilator. May be fatal if swallowed.
5. FIRE FIGHTING MEASURES:
All Kit Components
Suitable extinguishing Media: Water Spray, Carbon Dioxide, dry chemicals.
Unsuitable extinguishing Media: No data available.
Special exposure hazards: No generation of hazardous or toxic gases in dangerous
quantities.
Instructions: Due to small quantities, no special instructions apply.
LC802.11
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TEPNEL LIFECODES MOLECULAR DIAGNOSTICS
550 West Avenue ?Stamford, CT 06902
203.328.9500 ?888.329.0255 ?203.328.9598 fax
Special protective equipment for Firefighters: Due to small quantities, no special instructions apply.
6. ACCIDENTAL RELEASE MEASURES:
All Kit Components
Land Spill: Contain spill. Use personal protective equipment to prevent contact with eyes, skin and clothing.
Avoid routing to water bodies or streams. Pick up and place in containers for recovery and disposal.
Water Spill: Contain spill. Prevent eye contact. Prevent flow to water supplies. Isolate and take up for
disposal.
7. HANDLING AND STORAGE
All Kit Components
Handling: Observe normal hygiene standards. Discharge waste according to local regulations. Remove and
clean contaminated clothing. Handle open container with care.
Storage: Follow storage conditions on label. Keep container tightly closed. Keep away from heat sources,
LIGHT, combustible materials, acids and metals. Storage temperature: Consult component label.
8. EXPOSURE CONTROLS/PERSONAL PROTECTION
All Kit Components
Eye Protection: Safety glasses or face shield.
Hand Protection: Protective gloves.
Skin Protection: Protective clothing.
Eyewash and safety shower readily available in the immediate area.
9. PHYSICAL AND CHEMICAL PROPERTIES
All Kit Components
Liquid
Probe Mix
Gray
10. STABILITY AND REACTIVITY
All Kit Components
General: Stable when stored at specified conditions (see label) until expiry date noted on vial.
Reactivity/Hazardous Decomposition products:
Probe Mix pertains to Tetramethylammonium chloride, In fire conditions can emit carbon monoxide,
hydrochloric acid and nitrogen oxides.
11. TOXICOLOGICAL INFORMATION:
See section "2. Composition/Information on Ingredients" to identify kit components that contain the substances
mentioned in this section.
LC802.11
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TEPNEL LIFECODES MOLECULAR DIAGNOSTICS
550 West Avenue ?Stamford, CT 06902
203.328.9500 ?888.329.0255 ?203.328.9598 fax
Acute Toxicity:
Tetramethylammonium chloride LD50 for 50% solution = 94 mg/kg (oral-rat)
Not identified as an OSHA, NTP or IARC carcinogen. No data
available indicating any carcinogenic activity.
Chronic toxicity: Carcinogenicity (TLV) :A4
Routes of exposure:
Ingestion, inhalation, eyes and skin
Caution! Most components contain (a) substance(s) that are absorbed through the skin.
Acute effects/Symptoms:
Probe Mix contains tetramethylammonium chloride which may cause dizziness, nausea and shortness of breath
(nicotine-like symptoms) upon ingestion. All other components may be Harmful if swallowed.
Chronic Effects: See also chronic toxicity.
12. ECOLOGICAL INFORMATION
Aquatic toxicity:
Tetramethylammonium chloride: This material may be biodegradable in an acclimated biological treatment
facility. Reportedly: ~50% inhibition of nitrification at 2200 mg/L; Reportedly for dapnia magna: LOEC
(lowest observed effect concentration) = 0.05 mg/L; NOEC(no observed effect concentration) 0.03 mg/L.
Acute aquatic toxicity testing has shown this compound to be highly toxic to ceriodaphnia dubia (water flea).
13. WASTE DISPOSAL CONSIDERATIONS
Provision relating to waste: Hazardous waste (91/689/EC)
Packaging/container: Waste material code packaging (91/689/EEC, Council Decision 2001/118/EC, O/J. L47
of 16/2/2001: 15 01 10 packaging containing residues of contaminated substances)
Disposal methods:
Incinerate and/or dispose of according to all federal, state and local regulations, do not discharge directly into
surface waters.
14. TRANSPORT INFORMATION
No restrictions.
15. REGULATORY INFORMATION
Classifications according to directives 67/548/EEC and 1999/45/EC
Probe Mix contains tetramethylammonium chloride, which is classified as Harmful.
R25 Toxic if swallowed
R36/37/38 Irritating to eyes, respiratory system and skin
R21 Harmful in contact with skin
S26 Irritating to eyes, respiratory system and skin
S36/37 In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
S45 Wear suitable protective clothing and gloves
LC802.11
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TEPNEL LIFECODES MOLECULAR DIAGNOSTICS
550 West Avenue ?Stamford, CT 06902
203.328.9500 ?888.329.0255 ?203.328.9598 fax
16. OTHER INFORMATION
Tetramethylammonium chloride: RETCS Number: BS7700000
______________________________________________________________________
This information is believed to be accurate and represents the best information currently available to Tepnel
Lifecodes.
The use of the information contained herein is intended for persons having technical skill and at their own
discretion and risk. The information has been developed from sources considered to be reliable but has not
been independently verified by Tepnel Lifecodes. Therefore, Tepnel Lifecodes cannot guarantee the accuracy
of the information in these sources nor should statements contained herein, be considered an official
expression. NO REPRESENTATION OR WARRANTY, EXPRESSED OR IMPLIED, INCLUDING
WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE is made
with respect to the information contained herein. It is the user's obligation to determine the conditions of safe
use of this material. Tepnel Lifecodes urges the users of this product to study this MSDS and become aware of
any hazards associated with this material. In the interests of safety, the information herein should be made
available to your employees, agents and contractors who handle this material.
MSDS established: July 22, 2003
Revision number: 11
Revision date: 04/20/09
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