MATERIAL SAFETY DATA SHEET
1. Product Identification
Chemical Name: Aqueous solution
Trade Name: Acrylamide / Bis-acrylamide Solution
Synonyms: Electrophoresis gel solution. For Acrylamide, acrylic amide,
ethylenecarboxamide, propenamide, vinylamide. For
Formaldehyde, formalin.
2. Composition/Information of Ingredients
Ingredient Name CAS# % Ingredients EC Classifications
Water 7732-18-5 Not classified as dangerous
Acrylamide 79-06-1 1 - 30% T; R24/25 R48/23/24/25 Carc 2; R45 Mut2; R46
Crosslinker 110-26-9 < 10% T; R45-48/22/24/25
3. Hazards Identification
Emergency Overview: Clear, colourless aqueous solution
Potential Health Effects: Carcinogenic - acrylamide is a probable human carcinogen and human neurotoxin. Another minor component has been shown to cause cancer in laboratory animals and is also neurotoxic. May be irritating to eyes, skin and respiratory tract. Exposure to formaldehyde may produce allergic respiratory effects in sensitized individuals, including asthma, coughing, wheezing, shortness of breath. Formaldehyde is also considered a probable human carcinogen.
4. First Aid Measures
Eyes: Flush with water for at least fifteen minutes. If irritation occurs and persists, contact a medical doctor.
Skin: Remove contaminated clothing, and shoes, and wash thoroughly with soap and water. If irritation occurs and persists, contact a medical doctor.
Inhalation: Remove to fresh air. If breathing difficulty or discomfort occurs and persists, contact a medical doctor.
Ingestion: Drink 1 or 2 glasses of water and induce vomiting by touching the back of the throat with a finger or by giving syrup of ipecac. Never induce vomiting or give anything by mouth to an unconscious person. See a medical doctor.
Notes to Acrylamide/Bis-acrylamide Solutions are anticipated to be moderately irritating medical to the eyes and respiratory tract. Treatment is controlled removal of exposure doctor: with symptomatic and supportive care.
5. Fire Fighting Measures
Extinguishing Media: Use carbon dioxide, dry chemical powder, alcohol or polymer foram, or water spray.
Unusual Fire/Explosion Hazard: Negligible fire hazard when exposed to heat or flame.
Special Fire Fighting Procedures: Wear self-contained breathing apparatus and full protective clothing to prevent contact with skin and eyes.
Hazardous Decomposition Products: Burning may produce carbon monoxide and/or carbon dioxide, ammonia and nitrous oxides.
6. Accidental Release Measures
Absorb on absorbent material and place into closed containers for later disposal. Flush area with water. Material sublimes; therefore, clean up all spills before they dry. For personal precautions, see section 8.
7. Handling & Storage
Store in a cool (<25癈) dry area way from combustibles.
Long term storage at 4癈, preferably refrigerated.
8. Exposure Controls/Personal Protection
Prevention of work exposure: Avoid all contact. There shall be access to water and eye wash fountain. Careful personal hygiene is a necessity.
Respiratory protection: Wear NIOSH approved mask, or a mask with a gas/particle filter: A2/P2 in the absence of adequate ventilation.
Gloves: Chemical-resistant gloves should be worn.
Special Clothing & Equipment: Not required. Lab coat or equivalent recommended.
Eye Protection: Wear standard safety goggles or glasses.
Exposure Limits: None established for the formulation. Acrylamide: OSHA PEL = 0.3 mg/m?(skin) 1989; ACGIH TLV = 0.03 mg/m?(skin) 1994- 95, Australia (skin) 1990. 0.30 mg/m? Sweden (skin) 1984, United Kingdom (skin) 1991. Formaldehyde: 0.75 ppm PSHA PEL CFR 1900.1048, 0.3 ppm ceiling limit ACGOH TLV (1994- 95), 1 ppm TWA; 2ppm STEL Australia (sensitizer) 1990. 0.5ppm; 1 ppm for 5 minutes/8x shift Germany (sensitizer) 1989, 2ppm, 2ppm 10 minute STEL United Kingdom (sensitizer) 1991.
9. Physical/Chemical Properties
Appearance: Clear or colourless aqueous solution pH: 6.8
Odour: None Specify Gravity Not available
Melting Point: Not applicable % Volatile: 50-70% Water
Boiling Point: about 100 degrees C Vapour Density: Not determined
Flammable Limits in Air: Not available Vapour Pressure: Not determined
Explosive Properties: Not applicable Molecular Weight: Not applicable
Flash Point: Not measured Evaporation Rate: As water
Autoignition Temps: Not applicable Partition Coefficient: No data available
Oxidising Properties: Not applicable
Solubility (H20): Aqueous solution
10. Stability & Reactivity
Stability: Stable
Hazardous Decomposition Products: Under fire it can release toxic fumes of carbon monoxide, carbon dioxide, ammonia & nitrogen oxides.
Conditions/Materials to avoid May undergo polymerisation if exposed to heat or (incompatibility): ultraviolet light. If polymerisation occurs in a closed container, sufficient heat and pressure may be generated to rupture the container. Avoid strong oxidisers, acids, bases and vinyl polymerisation initiators. Also avoid contamination with iron, copper, aluminium, brass and bronze.
11. Toxicological Information
Skin Contact: No data available for formulation. Acrylamide: Mild to moderate irritant (rabbit) Doc.TLVs 1986, Gosselin 1984, Formaldehyde: Moderate irritant (rabbit), Mild irritant (human) RTECS 1985, Skin sensitizer IARC 1982.
Skin Absorption: No data available for formulation. Acrylamide: Dermal LD50 = 500 mg/kg IARC 1986. Formaldehyde: LD50 = 270 mg/kg (rabbit) RTECS 1985.
Inhalation: No data available for formulation. Formaldehyde: LC50 = 0.59 mg/L (rat), respiratory sensitizer, RTECS 1985, Doc TLVs 1991.
Ingestion: No data available for formulation. Acrylamide: Oral LD50 = 170 mg/kg (rat), RTECS 1983, Formaldehyde: Oral LD50 = 800 mg/kg (rat) RTECS 1985.
Eye Contact: No data available for formulation. Acrylamide: Moderate to sever irritation (rabbit), Doc TLVs 1986, Gosselin 1984, RTECS 1983. Formaldehyde: Severe irritant, TECS 1985.
Acute Effects from No data available for the formulation. Acrylamide is toxic by the oral
Overexposure and dermal routes of exposure. It is readily absorbed through the skin and by inhalation. Acrylamide is a human neurotoxin causing damage to both the central and peripheral nervous system. Symptoms include muscular weakness (particularly in the lower limbs), ataxia, tremors, numbness and tingling. Neurotoxicity produced by dermal exposure
may be preceded by peeling and redness of the skin on the hands and feet. Mild cases of poisoning appear to be reversible with time if exposure is terminated. Other adverse effects may include irritation to skin eyes and respiratory tract. Contact dermatitis has been reported with acrylamide. Formaldehyde causes irritation to the eyes, nose, throat and respiratory tract at low concentrations (<1 ppm). Difficulty in breathing may occur at 10 to 20 ppm, with serious injury to the respiratory tract at 50 ppm. Exposure to formaldehyde may produce allergic respiratory effects, including asthma to sensitised individuals.
Chronic Effects from No data available for the formulation. Testicular degeneration as well as Overexposure: reduced sperm motility, impaired fertility and dominant lethal effects with subchronic exposure to acrylamide have been reported in laboratory animals. Impairment of visual function was observed in female monkeys treated with acrylamide (10mg/kg bw). Acrylamide is classified as a suspect or probable human carcinogen by ACGIH, IARC and EPA. Acrylamide has produced chromosome damage as well as mutations in several in vitro and in vivo tests. Another component has also shown clear evidence of carcinogenicity in experimental animals in a 2 year NTP gavage study. Formaldehyde is listed as a probable human carcinogen by IARC, EPA, NTP, ACGIH, OSHA. It has caused mutations and chromosome damage in mammalian cells and in some in vivo tests. Repeated or long term exposure may produce allergic skin dermatitis as well as respiratory sensitisation. Workplace exposure to formaldehyde may cause disturbed sleep, unusual thirst; loss of smell, increased upper respiratory disease, chronic airway obstruction, breathing problems, menstrual disorders, pregnancy complications, low birth weight in addition to the irritant effects at level up to 4 ppm.
Exotoxicity: No data available for the formulation.
Carcinogenicity IARC NTP Other (OSHA) ACGIH
Yes, acrylamide Yes Yes No Yes
Yes, formaldehyde Yes Yes Yes Yes
Yes, proprietary component No Yes No No
12. Ecological Information
Environmental Fate: No data available
Environmental Effect: No data available
13. Disposal Considerations
Dispose in accordance with applicable local, state/provincial, and federal/national regulations.
14. Transportation Information
U.S. DOT/ ICAO/IATA/ Non Hazardous.
Canada (TDG).
15. Regulatory Information
U.S. TSCA Inventory: Formulation is not listed. Acrylamide and formaldehyde are listed.
U.S. ARA Title III: Not listed. Section 311/312: Not listed Section 313 (40 CFR 372): Not listed.
California Proposition 65: WARNING: This product contains chemicals known to the State of California to cause cancer.
Canada WHMIS: 2A, D. (Very toxic material, carcinogen).
EINECS No: (Acrylamide-201-173-7; Formaldehyde-200-001-8)
EC Symbols: T (Toxic)
EC Risk Phrases: R45 May cause cancer
R 46 May cause heritable genetic damage
R 24/25 Also toxic in contact with skin and if swallowed
R 48/23/24/25 Also toxic; danger of serious damage to health by prolonged exposure in contact with skin and if swallowed
R 36 Irritating to eyes
R 37 Irritating to respiratory system
R 38 Irritating to skin
EC Safety Advice Phrases: S 53 Avoid exposure - obtain special instructions before use
S 45 In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible)
Caution - this preparation contains substance(s) not yet fully tested.
16. Other Information
NFPA Designation Degree of Hazard Code
Fire 1 4 = Extreme
Health 2 3 = High
Reactivity 1 2 = Moderate
Special Hazard None 0 = Insignificant
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